Rmu_sc0000744.1_g000156

TITAN-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000744.1
OG0007112
Physical Location & Seq
Forward (+)
580324 .. 580824
501 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 501 bp
atggaggagaaggagaaacgatcaaaggctcattcgtggaaccctaaccctaaatccaacgccacagcaatgaagaagaagcacgacttcgagtattgcacggtttgcaagctgaatcacgaccaaggtccgagccacaagtacatccccagccacaccaagtccctctctactttcctctctcgcttccaggccaagctctccgacgtcgttttcttcctcaaaaaccctaccttcctccgcctcgaacacgccgctcgcaaccgcctctggtgcgtcttctgcgatttcgacattgacgagagagatagctccttcgcttgtggcaatgcaattaatcaccttgcgagtgccgaacatctgaagaatttgaagcacttcttgtggaaatacggtggaggaatggattctgtcgataaattcaattccttccaagggtccgaaagaggccagtggaaatgtgtatactggagcacttcctccttggtttgcagcagatga

Protein Analysis

166

Amino Acids

19.23

Weight (kDa)

9.18

Isoelectric Point (pI)

31.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCDC84 PF14968 32 - 142 5.8e-42 Coiled coil protein 84
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 210
AccBSI CCGCTC 1 cut(s) 257
AccI GTMKAC 1 cut(s) 465
AciI CCGC 3 cut(s) 241, 255, 265
AcsI RAATTY 2 cut(s) 367, 419
AcuI CTGAAG 1 cut(s) 383
AcyI GRCGYC 1 cut(s) 207
AfaI GTAC 1 cut(s) 143
AfiI CCNNNNNNNGG 1 cut(s) 435
AgsI TTSAA 2 cut(s) 373, 424
AjnI CCWGG 1 cut(s) 189
AluBI AGCT 3 cut(s) 112, 199, 312
AluI AGCT 3 cut(s) 112, 199, 312
Alw21I GWGCWC 1 cut(s) 476
AoxI GGCC 2 cut(s) 192, 448
ApeKI GCWGC 1 cut(s) 492
ApoI RAATTY 2 cut(s) 367, 419
AseI ATTAAT 1 cut(s) 336
Asp700I GAANNNNTTC 2 cut(s) 377, 406
AspS9I GGNCC 2 cut(s) 128, 438
AsuHPI GGTGA 1 cut(s) 332
AvaII GGWCC 2 cut(s) 128, 438
BbsI GAAGAC 1 cut(s) 271
Bbv12I GWGCWC 1 cut(s) 476
BciT130I CCWGG 1 cut(s) 191
BisI GCNGC 2 cut(s) 255, 493
BlsI GCNGC 2 cut(s) 256, 494
Bme1390I CCNGG 1 cut(s) 191
Bme18I GGWCC 2 cut(s) 128, 438
BmgT120I GGNCC 2 cut(s) 128, 438
BmiI GGNNCC 2 cut(s) 41, 439
BmrFI CCNGG 1 cut(s) 191
BoxI GACNNNNGTC 1 cut(s) 126
BpiI GAAGAC 1 cut(s) 271
BpmI CTGGAG 1 cut(s) 490
BsaHI GRCGYC 1 cut(s) 207
BsaJI CCNNGG 3 cut(s) 124, 433, 483
Bsc4I CCNNNNNNNGG 1 cut(s) 435
Bse1I ACTGG 2 cut(s) 451, 473
Bse3DI GCAATG 2 cut(s) 75, 334
BseBI CCWGG 1 cut(s) 191
BseDI CCNNGG 3 cut(s) 124, 433, 483
BseGI GGATG 1 cut(s) 144
BseLI CCNNNNNNNGG 1 cut(s) 435
BseMI GCAATG 2 cut(s) 75, 334
BseNI ACTGG 2 cut(s) 451, 473
BseRI GAGGAG 1 cut(s) 20
BseYI CCCAGC 1 cut(s) 149
BshFI GGCC 2 cut(s) 194, 450
BsiHKAI GWGCWC 1 cut(s) 476
BslFI GGGAC 1 cut(s) 148
BslI CCNNNNNNNGG 1 cut(s) 435
BsmFI GGGAC 1 cut(s) 148
BsnI GGCC 2 cut(s) 194, 450
Bsp1286I GDGCHC 1 cut(s) 476
Bsp143I GATC 1 cut(s) 20
BspACI CCGC 3 cut(s) 241, 255, 265
BspANI GGCC 2 cut(s) 194, 450
BspLI GGNNCC 2 cut(s) 41, 439
BsrBI CCGCTC 1 cut(s) 257
BsrDI GCAATG 2 cut(s) 75, 334
BsrI ACTGG 2 cut(s) 451, 473
BssECI CCNNGG 3 cut(s) 124, 433, 483
BssMI GATC 1 cut(s) 20
BssNAI GTATAC 1 cut(s) 466
BssNI GRCGYC 1 cut(s) 207
BssT1I CCWWGG 3 cut(s) 124, 433, 483
Bst1107I GTATAC 1 cut(s) 466
Bst2UI CCWGG 1 cut(s) 191
Bst4CI ACNGT 2 cut(s) 103, 395
BstACI GRCGYC 1 cut(s) 207
BstAPI GCANNNNNTGC 1 cut(s) 105
BstC8I GCNNGC 2 cut(s) 110, 259
BstF5I GGATG 1 cut(s) 144
BstKTI GATC 1 cut(s) 23
BstMBI GATC 1 cut(s) 20
BstMWI GCNNNNNNNGC 3 cut(s) 105, 273, 282
BstNI CCWGG 1 cut(s) 191
BstPAI GACNNNNGTC 1 cut(s) 126
BstSCI CCNGG 1 cut(s) 189
BstV2I GAAGAC 1 cut(s) 271
BstZ17I GTATAC 1 cut(s) 466
BsuRI GGCC 2 cut(s) 194, 450
BtsCI GGATG 1 cut(s) 144
BtsIMutI CAGTG 1 cut(s) 458
Cac8I GCNNGC 2 cut(s) 110, 259
Cfr13I GGNCC 2 cut(s) 128, 438
CseI GACGC 1 cut(s) 265
Csp6I GTAC 1 cut(s) 142
CviJI RGCY 8 cut(s) 29, 112, 135, 153, 194, 199, 312, 450
CviKI_1 RGCY 8 cut(s) 29, 112, 135, 153, 194, 199, 312, 450
CviQI GTAC 1 cut(s) 142
DpnI GATC 1 cut(s) 22
DpnII GATC 1 cut(s) 20
EciI GGCGGA 1 cut(s) 230
Eco130I CCWWGG 3 cut(s) 124, 433, 483
Eco47I GGWCC 2 cut(s) 128, 438
Eco57I CTGAAG 1 cut(s) 383
EcoRII CCWGG 1 cut(s) 189
EcoT14I CCWWGG 3 cut(s) 124, 433, 483
ErhI CCWWGG 3 cut(s) 124, 433, 483
FaiI YATR 1 cut(s) 466
FalI AAGNNNNNCTT 4 cut(s) 71, 103, 365, 397
FaqI GGGAC 1 cut(s) 148
FblI GTMKAC 1 cut(s) 465
Fnu4HI GCNGC 2 cut(s) 255, 493
FokI GGATG 1 cut(s) 131
Fsp4HI GCNGC 2 cut(s) 255, 493
GluI GCNGC 2 cut(s) 255, 493
GsaI CCCAGC 1 cut(s) 153
GsuI CTGGAG 1 cut(s) 490
HaeIII GGCC 2 cut(s) 194, 450
HgaI GACGC 1 cut(s) 265
Hin1I GRCGYC 1 cut(s) 207
HinfI GANTC 2 cut(s) 115, 407
HphI GGTGA 1 cut(s) 332
Hpy166II GTNNAC 1 cut(s) 466
Hpy188I TCNGA 4 cut(s) 132, 205, 363, 442
Hpy188III TCNNGA 1 cut(s) 119
Hpy8I GTNNAC 1 cut(s) 466
Hpy99I CGWCG 2 cut(s) 209, 212
HpyAV CCTTC 4 cut(s) 4, 244, 325, 439
HpyCH4III ACNGT 2 cut(s) 103, 395
HpyCH4IV ACGT 1 cut(s) 207
HpyCH4V TGCA 4 cut(s) 99, 108, 332, 492
HpyF10VI GCNNNNNNNGC 3 cut(s) 105, 273, 282
HpySE526I ACGT 1 cut(s) 207
Hsp92I GRCGYC 1 cut(s) 207
Kzo9I GATC 1 cut(s) 20
LmnI GCTCC 2 cut(s) 317, 471
LpnPI CCDG 6 cut(s) 163, 176, 203, 256, 454, 464
MaeII ACGT 1 cut(s) 207
MalI GATC 1 cut(s) 22
MbiI CCGCTC 1 cut(s) 257
MboI GATC 1 cut(s) 20
MboII GAAGA 5 cut(s) 85, 88, 208, 271, 376
MhlI GDGCHC 1 cut(s) 476
MluCI AATT 4 cut(s) 333, 367, 419, 424
MmeI TCCRAC 2 cut(s) 81, 228
MnlI CCTC 9 cut(s) 176, 188, 230, 248, 254, 278, 392, 440, 490
MroXI GAANNNNTTC 2 cut(s) 377, 406
MseI TTAA 1 cut(s) 336
MslI CAYNNNNRTG 1 cut(s) 68
MspR9I CCNGG 1 cut(s) 191
MvaI CCWGG 1 cut(s) 191
MwoI GCNNNNNNNGC 3 cut(s) 105, 273, 282
NdeII GATC 1 cut(s) 20
NlaIV GGNNCC 2 cut(s) 41, 439
PcsI WCGNNNNNNNCGW 1 cut(s) 297
PdmI GAANNNNTTC 2 cut(s) 377, 406
PfeI GAWTC 2 cut(s) 115, 407
PkrI GCNGC 2 cut(s) 256, 494
PshAI GACNNNNGTC 1 cut(s) 126
PshBI ATTAAT 1 cut(s) 336
Psp6I CCWGG 1 cut(s) 189
PspFI CCCAGC 1 cut(s) 149
PspGI CCWGG 1 cut(s) 189
PspN4I GGNNCC 2 cut(s) 41, 439
PspPI GGNCC 2 cut(s) 128, 438
RsaI GTAC 1 cut(s) 143
RsaNI GTAC 1 cut(s) 142
RseI CAYNNNNRTG 1 cut(s) 68
SaqAI TTAA 1 cut(s) 336
SatI GCNGC 2 cut(s) 255, 493
Sau3AI GATC 1 cut(s) 20
Sau96I GGNCC 2 cut(s) 128, 438
ScrFI CCNGG 1 cut(s) 191
SduI GDGCHC 1 cut(s) 476
SetI ASST 7 cut(s) 114, 130, 201, 210, 236, 314, 345
SinI GGWCC 2 cut(s) 128, 438
SmiMI CAYNNNNRTG 1 cut(s) 68
Sse9I AATT 4 cut(s) 333, 367, 419, 424
SsiI CCGC 3 cut(s) 241, 255, 265
StyD4I CCNGG 1 cut(s) 189
StyI CCWWGG 3 cut(s) 124, 433, 483
TaaI ACNGT 2 cut(s) 103, 395
TaiI ACGT 1 cut(s) 210
TaqI TCGA 4 cut(s) 90, 246, 291, 414
TasI AATT 4 cut(s) 333, 367, 419, 424
TatI WGTACW 1 cut(s) 141
TauI GCSGC 1 cut(s) 257
TfiI GAWTC 2 cut(s) 115, 407
Tru1I TTAA 1 cut(s) 336
Tru9I TTAA 1 cut(s) 336
TscAI CASTG 1 cut(s) 458
TseI GCWGC 1 cut(s) 492
TspDTI ATGAA 1 cut(s) 86
TspRI CASTG 1 cut(s) 458
VpaK11BI GGWCC 2 cut(s) 128, 438
VspI ATTAAT 1 cut(s) 336
XapI RAATTY 2 cut(s) 367, 419
XmiI GTMKAC 1 cut(s) 465
XmnI GAANNNNTTC 2 cut(s) 377, 406
ZraI GACGTC 1 cut(s) 208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.