Rmu_sc0000982.1_g000066

AMMECR1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000982.1
OG0009734
Physical Location & Seq
Forward (+)
275392 .. 277354
1963 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 861 bp
atgtccctctctctccctcccgttatccaatcccgtctcggctctctctctctctgcctcgcctccgtccgcaccttcaaagagcacacctaccgcgtctactccgccctcttcgcctcagtctccagcgactgcactgcacgcgtcggggacgtgcatgagcctagatccaccatgatcatcccggcgcatgagtttgagatcctctcctgtgattggaacagtataacaactgcgtcatcgtcattgcctccgtcgacaaatcgatcaaggtttgggacgccaggtccatcggagcttcgatttgcttctcaacggccacagctacgccgtcagaaaagccaaattctcgccgcattggcaaagcttgatgacgtcgtgctcgtaggacttgtcagttccgttgcttcaatactagtaaggactagggattggcgtcagaaaatgatggacatactgaagagatttaattctgaagaagaagctgatggaatggatgaggatgttgggaagcatggtataatcattgagtttactgatctgggctatggtacaaggcgcaatgcaacatacttgcctgaggttgctgcgcatgaaggttggactcaaatagaagcaattgactcattgatgcggaaagcagggtatactgggaccatcaccgagtcacttcggaagcgtatacgattgacccgctggatttttcgtggtcgttcccgatttctcttttatggcgaccctgtgaatcctgataatcctcaattcgaccgagagtcctggtttaaagctcatagcgaggtgatagcgattaacagtgacttgattagtgacatggtcagttacttagttagtgacatgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006950 GO:0006970 GO:0008150 GO:0009628 GO:0009651 GO:0044424 GO:0044444 GO:0044464 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMMECR1

Protein Analysis

286

Amino Acids

32.24

Weight (kDa)

7.76

Isoelectric Point (pI)

55.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMMECR1 PF01871 166 - 232 1.7e-11 AMMECR1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 378
Acc16I TGCGCA 1 cut(s) 591
AccI GTMKAC 4 cut(s) 99, 257, 647, 682
AccII CGCG 2 cut(s) 96, 144
AciI CCGC 6 cut(s) 70, 94, 105, 354, 634, 694
AclWI GGATC 2 cut(s) 162, 196
AcoI YGGCCR 1 cut(s) 317
AcsI RAATTY 1 cut(s) 345
AcuI CTGAAG 2 cut(s) 479, 495
AcyI GRCGYC 3 cut(s) 281, 375, 436
AfaI GTAC 1 cut(s) 553
AfiI CCNNNNNNNGG 1 cut(s) 216
AflIII ACRYGT 2 cut(s) 142, 855
AgsI TTSAA 2 cut(s) 79, 411
AhdI GACNNNNNGTC 2 cut(s) 285, 772
AhlI ACTAGT 1 cut(s) 415
AjiI CACGTC 1 cut(s) 154
AjnI CCWGG 2 cut(s) 283, 776
AluBI AGCT 5 cut(s) 298, 325, 367, 485, 788
AluI AGCT 5 cut(s) 298, 325, 367, 485, 788
Alw21I GWGCWC 2 cut(s) 87, 384
Alw26I GTCTC 2 cut(s) 41, 127
AlwI GGATC 2 cut(s) 162, 196
AlwNI CAGNNNCTG 1 cut(s) 132
AoxI GGCC 1 cut(s) 317
ApeKI GCWGC 1 cut(s) 587
ApoI RAATTY 1 cut(s) 345
AspLEI GCGC 3 cut(s) 190, 561, 592
AspS9I GGNCC 2 cut(s) 287, 654
AsuC2I CCSGG 1 cut(s) 185
AsuHPI GGTGA 2 cut(s) 652, 811
AvaII GGWCC 2 cut(s) 287, 654
AxyI CCTNAGG 1 cut(s) 579
Bbv12I GWGCWC 2 cut(s) 87, 384
BbvI GCAGC 1 cut(s) 574
BccI CCATC 4 cut(s) 298, 442, 482, 665
BceAI ACGGC 2 cut(s) 315, 332
BcgI CGANNNNNNTGC 2 cut(s) 119, 153
BciT130I CCWGG 2 cut(s) 285, 778
BclI TGATCA 1 cut(s) 177
BcnI CCSGG 1 cut(s) 185
BcoDI GTCTC 2 cut(s) 41, 127
BcuI ACTAGT 1 cut(s) 415
BfaI CTAG 3 cut(s) 165, 416, 426
BglI GCCNNNNNGGC 1 cut(s) 359
BisI GCNGC 2 cut(s) 354, 588
BlsI GCNGC 2 cut(s) 355, 589
Bme1390I CCNGG 3 cut(s) 185, 285, 778
Bme18I GGWCC 2 cut(s) 287, 654
BmeRI GACNNNNNGTC 2 cut(s) 285, 772
BmgBI CACGTC 1 cut(s) 154
BmgT120I GGNCC 2 cut(s) 287, 654
BmiI GGNNCC 1 cut(s) 655
BmrFI CCNGG 3 cut(s) 185, 285, 778
BmrI ACTGGG 1 cut(s) 660
BmsI GCATC 1 cut(s) 621
BmuI ACTGGG 1 cut(s) 660
BplI GAGNNNNNCTC 2 cut(s) 191, 223
BpmI CTGGAG 1 cut(s) 109
BpuMI CCSGG 1 cut(s) 185
Bsa29I ATCGAT 1 cut(s) 265
BsaHI GRCGYC 3 cut(s) 281, 375, 436
Bsc4I CCNNNNNNNGG 1 cut(s) 216
Bse1I ACTGG 1 cut(s) 655
Bse21I CCTNAGG 1 cut(s) 579
Bse3DI GCAATG 2 cut(s) 245, 568
BseBI CCWGG 2 cut(s) 285, 778
BseCI ATCGAT 1 cut(s) 265
BseGI GGATG 3 cut(s) 180, 502, 508
BseLI CCNNNNNNNGG 1 cut(s) 216
BseMI GCAATG 2 cut(s) 245, 568
BseMII CTCAG 2 cut(s) 132, 570
BseNI ACTGG 1 cut(s) 655
BseXI GCAGC 1 cut(s) 574
BsgI GTGCAG 2 cut(s) 118, 123
Bsh1236I CGCG 2 cut(s) 96, 144
Bsh1285I CGRYCG 1 cut(s) 769
BshFI GGCC 1 cut(s) 319
BshVI ATCGAT 1 cut(s) 265
BsiEI CGRYCG 1 cut(s) 769
BsiHKAI GWGCWC 2 cut(s) 87, 384
BsiSI CCGG 1 cut(s) 185
BslFI GGGAC 3 cut(s) 164, 292, 667
BslI CCNNNNNNNGG 1 cut(s) 216
BsmAI GTCTC 2 cut(s) 41, 127
BsmBI CGTCTC 1 cut(s) 41
BsmFI GGGAC 3 cut(s) 164, 292, 667
BsnI GGCC 1 cut(s) 319
Bsp1286I GDGCHC 2 cut(s) 87, 384
Bsp143I GATC 5 cut(s) 167, 177, 201, 266, 538
BspACI CCGC 6 cut(s) 70, 94, 105, 354, 634, 694
BspANI GGCC 1 cut(s) 319
BspCNI CTCAG 2 cut(s) 131, 571
BspDI ATCGAT 1 cut(s) 265
BspFNI CGCG 2 cut(s) 96, 144
BspLI GGNNCC 1 cut(s) 655
BspPI GGATC 2 cut(s) 162, 196
BsrDI GCAATG 2 cut(s) 245, 568
BsrI ACTGG 1 cut(s) 655
BssMI GATC 5 cut(s) 167, 177, 201, 266, 538
BssNAI GTATAC 2 cut(s) 648, 683
BssNI GRCGYC 3 cut(s) 281, 375, 436
Bst1107I GTATAC 2 cut(s) 648, 683
Bst2UI CCWGG 2 cut(s) 285, 778
Bst4CI ACNGT 2 cut(s) 224, 815
Bst6I CTCTTC 2 cut(s) 116, 455
BstACI GRCGYC 3 cut(s) 281, 375, 436
BstC8I GCNNGC 1 cut(s) 142
BstDEI CTNAG 3 cut(s) 118, 579, 844
BstF5I GGATG 3 cut(s) 180, 502, 508
BstFNI CGCG 2 cut(s) 96, 144
BstHHI GCGC 3 cut(s) 190, 561, 592
BstKTI GATC 5 cut(s) 170, 180, 204, 269, 541
BstMAI GTCTC 2 cut(s) 41, 127
BstMBI GATC 5 cut(s) 167, 177, 201, 266, 538
BstMCI CGRYCG 1 cut(s) 769
BstMWI GCNNNNNNNGC 3 cut(s) 113, 141, 359
BstNI CCWGG 2 cut(s) 285, 778
BstNSI RCATGY 1 cut(s) 859
BstSCI CCNGG 3 cut(s) 183, 283, 776
BstUI CGCG 2 cut(s) 96, 144
BstV1I GCAGC 1 cut(s) 574
BstX2I RGATCY 2 cut(s) 167, 201
BstYI RGATCY 2 cut(s) 167, 201
BstZ17I GTATAC 2 cut(s) 648, 683
Bsu15I ATCGAT 1 cut(s) 265
Bsu36I CCTNAGG 1 cut(s) 579
BsuRI GGCC 1 cut(s) 319
BsuTUI ATCGAT 1 cut(s) 265
BtrI CACGTC 1 cut(s) 154
BtsCI GGATG 3 cut(s) 180, 502, 508
BtsI GCAGTG 1 cut(s) 135
BtsIMutI CAGTG 2 cut(s) 135, 820
Cac8I GCNNGC 1 cut(s) 142
CaiI CAGNNNCTG 1 cut(s) 132
CfoI GCGC 3 cut(s) 190, 561, 592
Cfr13I GGNCC 2 cut(s) 287, 654
ClaI ATCGAT 1 cut(s) 265
CseI GACGC 5 cut(s) 85, 133, 225, 289, 425
Csp6I GTAC 1 cut(s) 552
CviAII CATG 7 cut(s) 158, 175, 191, 515, 593, 832, 856
CviQI GTAC 1 cut(s) 552
DdeI CTNAG 3 cut(s) 118, 579, 844
DpnI GATC 5 cut(s) 169, 179, 203, 268, 540
DpnII GATC 5 cut(s) 167, 177, 201, 266, 538
DraI TTTAAA 1 cut(s) 784
DriI GACNNNNNGTC 2 cut(s) 285, 772
EaeI YGGCCR 1 cut(s) 317
Eam1104I CTCTTC 2 cut(s) 116, 455
Eam1105I GACNNNNNGTC 2 cut(s) 285, 772
EarI CTCTTC 2 cut(s) 116, 455
EciI GGCGGA 1 cut(s) 94
Eco47I GGWCC 2 cut(s) 287, 654
Eco57I CTGAAG 2 cut(s) 479, 495
Eco81I CCTNAGG 1 cut(s) 579
EcoRII CCWGG 2 cut(s) 283, 776
Esp3I CGTCTC 1 cut(s) 41
FaeI CATG 7 cut(s) 161, 178, 194, 518, 596, 835, 859
FaqI GGGAC 3 cut(s) 164, 292, 667
FatI CATG 7 cut(s) 157, 174, 190, 514, 592, 831, 855
FauI CCCGC 1 cut(s) 701
FbaI TGATCA 1 cut(s) 177
FblI GTMKAC 4 cut(s) 99, 257, 647, 682
Fnu4HI GCNGC 2 cut(s) 354, 588
FokI GGATG 3 cut(s) 167, 509, 515
Fsp4HI GCNGC 2 cut(s) 354, 588
FspBI CTAG 3 cut(s) 165, 416, 426
FspI TGCGCA 1 cut(s) 591
GlaI GCGC 3 cut(s) 189, 560, 591
GluI GCNGC 2 cut(s) 354, 588
GsuI CTGGAG 1 cut(s) 109
HaeIII GGCC 1 cut(s) 319
HapII CCGG 1 cut(s) 185
HgaI GACGC 5 cut(s) 85, 133, 225, 289, 425
HhaI GCGC 3 cut(s) 190, 561, 592
Hin1I GRCGYC 3 cut(s) 281, 375, 436
Hin1II CATG 7 cut(s) 161, 178, 194, 518, 596, 835, 859
Hin6I GCGC 3 cut(s) 188, 559, 590
HinP1I GCGC 3 cut(s) 188, 559, 590
HincII GTYRAC 1 cut(s) 258
HindII GTYRAC 1 cut(s) 258
HindIII AAGCTT 1 cut(s) 365
HinfI GANTC 5 cut(s) 604, 623, 665, 745, 773
HpaII CCGG 1 cut(s) 185
HphI GGTGA 2 cut(s) 652, 811
Hpy166II GTNNAC 5 cut(s) 100, 258, 534, 648, 683
Hpy188I TCNGA 5 cut(s) 295, 336, 441, 475, 675
Hpy188III TCNNGA 2 cut(s) 717, 749
Hpy8I GTNNAC 5 cut(s) 100, 258, 534, 648, 683
Hpy99I CGWCG 3 cut(s) 149, 259, 380
HpyAV CCTTC 2 cut(s) 85, 590
HpyCH4III ACNGT 2 cut(s) 224, 815
HpyCH4IV ACGT 2 cut(s) 153, 375
HpyCH4V TGCA 4 cut(s) 135, 140, 157, 566
HpyF10VI GCNNNNNNNGC 3 cut(s) 113, 141, 359
HpyF3I CTNAG 3 cut(s) 118, 579, 844
HpySE526I ACGT 2 cut(s) 153, 375
Hsp92I GRCGYC 3 cut(s) 281, 375, 436
Hsp92II CATG 7 cut(s) 161, 178, 194, 518, 596, 835, 859
HspAI GCGC 3 cut(s) 188, 559, 590
Ksp22I TGATCA 1 cut(s) 177
Kzo9I GATC 5 cut(s) 167, 177, 201, 266, 538
LmnI GCTCC 1 cut(s) 295
Lsp1109I GCAGC 1 cut(s) 574
LweI GCATC 1 cut(s) 621
MaeI CTAG 3 cut(s) 165, 416, 426
MaeII ACGT 2 cut(s) 153, 375
MaeIII GTNAC 5 cut(s) 666, 815, 827, 839, 851
MalI GATC 5 cut(s) 169, 179, 203, 268, 540
MboI GATC 5 cut(s) 167, 177, 201, 266, 538
MboII GAAGA 4 cut(s) 103, 472, 488, 491
MfeI CAATTG 1 cut(s) 618
MflI RGATCY 2 cut(s) 167, 201
MhlI GDGCHC 2 cut(s) 87, 384
MluCI AATT 4 cut(s) 345, 469, 618, 761
MluI ACGCGT 1 cut(s) 142
MlyI GAGTC 4 cut(s) 598, 617, 674, 782
MmeI TCCRAC 1 cut(s) 581
MseI TTAA 3 cut(s) 468, 783, 810
MspA1I CMGCKG 1 cut(s) 696
MspI CCGG 1 cut(s) 185
MspR9I CCNGG 3 cut(s) 185, 285, 778
MunI CAATTG 1 cut(s) 618
MvaI CCWGG 2 cut(s) 285, 778
MvnI CGCG 2 cut(s) 96, 144
MwoI GCNNNNNNNGC 3 cut(s) 113, 141, 359
NciI CCSGG 1 cut(s) 185
NdeII GATC 5 cut(s) 167, 177, 201, 266, 538
NlaIII CATG 7 cut(s) 161, 178, 194, 518, 596, 835, 859
NlaIV GGNNCC 1 cut(s) 655
NmeAIII GCCGAG 1 cut(s) 18
NmuCI GTSAC 4 cut(s) 666, 815, 827, 851
NsbI TGCGCA 1 cut(s) 591
NspI RCATGY 1 cut(s) 859
PciI ACATGT 1 cut(s) 855
PcsI WCGNNNNNNNCGW 1 cut(s) 381
PfeI GAWTC 1 cut(s) 745
PflFI GACNNNGTC 1 cut(s) 833
PkrI GCNGC 2 cut(s) 355, 589
PleI GAGTC 4 cut(s) 598, 617, 673, 781
PpsI GAGTC 4 cut(s) 598, 617, 673, 781
PscI ACATGT 1 cut(s) 855
Psp6I CCWGG 2 cut(s) 283, 776
PspGI CCWGG 2 cut(s) 283, 776
PspN4I GGNNCC 1 cut(s) 655
PspPI GGNCC 2 cut(s) 287, 654
PstNI CAGNNNCTG 1 cut(s) 132
PsuI RGATCY 2 cut(s) 167, 201
PsyI GACNNNGTC 1 cut(s) 833
RsaI GTAC 1 cut(s) 553
RsaNI GTAC 1 cut(s) 552
SalI GTCGAC 1 cut(s) 256
SaqAI TTAA 3 cut(s) 468, 783, 810
SatI GCNGC 2 cut(s) 354, 588
Sau3AI GATC 5 cut(s) 167, 177, 201, 266, 538
Sau96I GGNCC 2 cut(s) 287, 654
SchI GAGTC 4 cut(s) 598, 617, 674, 782
ScrFI CCNGG 3 cut(s) 185, 285, 778
SduI GDGCHC 2 cut(s) 87, 384
SfaNI GCATC 1 cut(s) 621
SinI GGWCC 2 cut(s) 287, 654
SpeI ACTAGT 1 cut(s) 415
Sse9I AATT 4 cut(s) 345, 469, 618, 761
SsiI CCGC 6 cut(s) 70, 94, 105, 354, 634, 694
SspMI CTAG 3 cut(s) 165, 416, 426
StyD4I CCNGG 3 cut(s) 183, 283, 776
TaaI ACNGT 2 cut(s) 224, 815
TaiI ACGT 2 cut(s) 156, 378
TaqI TCGA 4 cut(s) 257, 265, 301, 765
TaqII GACCGA 1 cut(s) 783
TasI AATT 4 cut(s) 345, 469, 618, 761
TauI GCSGC 1 cut(s) 356
TfiI GAWTC 1 cut(s) 745
Tru1I TTAA 3 cut(s) 468, 783, 810
Tru9I TTAA 3 cut(s) 468, 783, 810
TscAI CASTG 2 cut(s) 142, 820
TseFI GTSAC 4 cut(s) 666, 815, 827, 851
TseI GCWGC 1 cut(s) 587
Tsp45I GTSAC 4 cut(s) 666, 815, 827, 851
TspDTI ATGAA 1 cut(s) 609
TspGWI ACGGA 3 cut(s) 55, 243, 391
TspRI CASTG 2 cut(s) 142, 820
Tth111I GACNNNGTC 1 cut(s) 833
VpaK11BI GGWCC 2 cut(s) 287, 654
XapI RAATTY 1 cut(s) 345
XceI RCATGY 1 cut(s) 859
XmiI GTMKAC 4 cut(s) 99, 257, 647, 682
XspI CTAG 3 cut(s) 165, 416, 426
ZraI GACGTC 1 cut(s) 376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.