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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 226 |
| AcsI | RAATTY | 2 cut(s) | 281, 293 |
| AfaI | GTAC | 1 cut(s) | 113 |
| AgsI | TTSAA | 1 cut(s) | 241 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 153, 185 |
| AluBI | AGCT | 2 cut(s) | 20, 26 |
| AluI | AGCT | 2 cut(s) | 20, 26 |
| Alw26I | GTCTC | 1 cut(s) | 157 |
| ApeKI | GCWGC | 1 cut(s) | 44 |
| ApoI | RAATTY | 2 cut(s) | 281, 293 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 270 |
| AspS9I | GGNCC | 1 cut(s) | 178 |
| AvaII | GGWCC | 1 cut(s) | 178 |
| BbsI | GAAGAC | 2 cut(s) | 138, 341 |
| BbvI | GCAGC | 1 cut(s) | 56 |
| BccI | CCATC | 2 cut(s) | 207, 361 |
| BcoDI | GTCTC | 1 cut(s) | 157 |
| BfaI | CTAG | 1 cut(s) | 203 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 53 |
| BisI | GCNGC | 1 cut(s) | 45 |
| BlsI | GCNGC | 1 cut(s) | 46 |
| Bme18I | GGWCC | 1 cut(s) | 178 |
| BmgT120I | GGNCC | 1 cut(s) | 178 |
| BpiI | GAAGAC | 2 cut(s) | 138, 341 |
| BpmI | CTGGAG | 1 cut(s) | 92 |
| BpuEI | CTTGAG | 1 cut(s) | 6 |
| BsaI | GGTCTC | 1 cut(s) | 157 |
| Bse1I | ACTGG | 1 cut(s) | 109 |
| BseGI | GGATG | 2 cut(s) | 75, 103 |
| BseNI | ACTGG | 1 cut(s) | 109 |
| BseXI | GCAGC | 1 cut(s) | 56 |
| BsmAI | GTCTC | 1 cut(s) | 157 |
| Bso31I | GGTCTC | 1 cut(s) | 157 |
| Bsp143I | GATC | 1 cut(s) | 309 |
| BspACI | CCGC | 1 cut(s) | 226 |
| BspTNI | GGTCTC | 1 cut(s) | 157 |
| BsrI | ACTGG | 1 cut(s) | 109 |
| BssMI | GATC | 1 cut(s) | 309 |
| Bst4CI | ACNGT | 1 cut(s) | 10 |
| BstC8I | GCNNGC | 1 cut(s) | 18 |
| BstDEI | CTNAG | 1 cut(s) | 49 |
| BstF5I | GGATG | 2 cut(s) | 75, 103 |
| BstKTI | GATC | 1 cut(s) | 312 |
| BstMAI | GTCTC | 1 cut(s) | 157 |
| BstMBI | GATC | 1 cut(s) | 309 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 32, 41, 53, 62 |
| BstV1I | GCAGC | 1 cut(s) | 56 |
| BstV2I | GAAGAC | 2 cut(s) | 138, 341 |
| BtgZI | GCGATG | 1 cut(s) | 335 |
| BtsCI | GGATG | 2 cut(s) | 75, 103 |
| Cac8I | GCNNGC | 1 cut(s) | 18 |
| Cfr13I | GGNCC | 1 cut(s) | 178 |
| Csp6I | GTAC | 1 cut(s) | 112 |
| CviJI | RGCY | 5 cut(s) | 20, 26, 47, 145, 222 |
| CviKI_1 | RGCY | 5 cut(s) | 20, 26, 47, 145, 222 |
| CviQI | GTAC | 1 cut(s) | 112 |
| DdeI | CTNAG | 1 cut(s) | 49 |
| DpnI | GATC | 1 cut(s) | 311 |
| DpnII | GATC | 1 cut(s) | 309 |
| Eco31I | GGTCTC | 1 cut(s) | 157 |
| Eco47I | GGWCC | 1 cut(s) | 178 |
| EcoRI | GAATTC | 1 cut(s) | 293 |
| FaiI | YATR | 5 cut(s) | 93, 95, 122, 219, 300 |
| FauNDI | CATATG | 1 cut(s) | 93 |
| Fnu4HI | GCNGC | 1 cut(s) | 45 |
| FokI | GGATG | 2 cut(s) | 62, 110 |
| Fsp4HI | GCNGC | 1 cut(s) | 45 |
| FspBI | CTAG | 1 cut(s) | 203 |
| GluI | GCNGC | 1 cut(s) | 45 |
| GsuI | CTGGAG | 1 cut(s) | 92 |
| HindIII | AAGCTT | 1 cut(s) | 24 |
| HinfI | GANTC | 4 cut(s) | 85, 101, 185, 337 |
| Hpy188I | TCNGA | 1 cut(s) | 265 |
| Hpy188III | TCNNGA | 2 cut(s) | 197, 251 |
| HpyAV | CCTTC | 1 cut(s) | 135 |
| HpyCH4III | ACNGT | 1 cut(s) | 10 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 32, 41, 53, 62 |
| HpyF3I | CTNAG | 1 cut(s) | 49 |
| Kzo9I | GATC | 1 cut(s) | 309 |
| LmnI | GCTCC | 1 cut(s) | 111 |
| LpnPI | CCDG | 5 cut(s) | 47, 122, 151, 203, 210 |
| Lsp1109I | GCAGC | 1 cut(s) | 56 |
| MaeI | CTAG | 1 cut(s) | 203 |
| MaeIII | GTNAC | 1 cut(s) | 182 |
| MalI | GATC | 1 cut(s) | 311 |
| MboI | GATC | 1 cut(s) | 309 |
| MboII | GAAGA | 4 cut(s) | 140, 143, 173, 346 |
| MluCI | AATT | 2 cut(s) | 281, 293 |
| MlyI | GAGTC | 3 cut(s) | 79, 179, 331 |
| MnlI | CCTC | 2 cut(s) | 46, 233 |
| MroXI | GAANNNNTTC | 1 cut(s) | 270 |
| MseI | TTAA | 2 cut(s) | 279, 287 |
| MslI | CAYNNNNRTG | 2 cut(s) | 80, 96 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 32, 41, 53, 62 |
| NdeI | CATATG | 1 cut(s) | 93 |
| NdeII | GATC | 1 cut(s) | 309 |
| NmuCI | GTSAC | 1 cut(s) | 182 |
| PdmI | GAANNNNTTC | 1 cut(s) | 270 |
| PfeI | GAWTC | 1 cut(s) | 101 |
| PkrI | GCNGC | 1 cut(s) | 46 |
| PleI | GAGTC | 3 cut(s) | 79, 179, 331 |
| PpsI | GAGTC | 3 cut(s) | 79, 179, 331 |
| PspPI | GGNCC | 1 cut(s) | 178 |
| RsaI | GTAC | 1 cut(s) | 113 |
| RsaNI | GTAC | 1 cut(s) | 112 |
| RseI | CAYNNNNRTG | 2 cut(s) | 80, 96 |
| SaqAI | TTAA | 2 cut(s) | 279, 287 |
| SatI | GCNGC | 1 cut(s) | 45 |
| Sau3AI | GATC | 1 cut(s) | 309 |
| Sau96I | GGNCC | 1 cut(s) | 178 |
| SchI | GAGTC | 3 cut(s) | 79, 179, 331 |
| SetI | ASST | 4 cut(s) | 22, 28, 180, 204 |
| SinI | GGWCC | 1 cut(s) | 178 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 80, 96 |
| SmlI | CTYRAG | 1 cut(s) | 21 |
| SmoI | CTYRAG | 1 cut(s) | 21 |
| Sse9I | AATT | 2 cut(s) | 281, 293 |
| SsiI | CCGC | 1 cut(s) | 226 |
| SspMI | CTAG | 1 cut(s) | 203 |
| TaaI | ACNGT | 1 cut(s) | 10 |
| TaqI | TCGA | 1 cut(s) | 159 |
| TasI | AATT | 2 cut(s) | 281, 293 |
| TfiI | GAWTC | 1 cut(s) | 101 |
| Tru1I | TTAA | 2 cut(s) | 279, 287 |
| Tru9I | TTAA | 2 cut(s) | 279, 287 |
| TseFI | GTSAC | 1 cut(s) | 182 |
| TseI | GCWGC | 1 cut(s) | 44 |
| Tsp45I | GTSAC | 1 cut(s) | 182 |
| TspDTI | ATGAA | 2 cut(s) | 137, 141 |
| TspGWI | ACGGA | 1 cut(s) | 170 |
| VpaK11BI | GGWCC | 1 cut(s) | 178 |
| XapI | RAATTY | 2 cut(s) | 281, 293 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 145 |
| XmnI | GAANNNNTTC | 1 cut(s) | 270 |
| XspI | CTAG | 1 cut(s) | 203 |