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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 226 |
| AgsI | TTSAA | 2 cut(s) | 200, 241 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 153, 185 |
| AluBI | AGCT | 2 cut(s) | 20, 26 |
| AluI | AGCT | 2 cut(s) | 20, 26 |
| Alw26I | GTCTC | 1 cut(s) | 157 |
| BbsI | GAAGAC | 1 cut(s) | 138 |
| BccI | CCATC | 1 cut(s) | 80 |
| BcoDI | GTCTC | 1 cut(s) | 157 |
| BfaI | CTAG | 1 cut(s) | 203 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 53 |
| BmsI | GCATC | 1 cut(s) | 299 |
| BpiI | GAAGAC | 1 cut(s) | 138 |
| BpmI | CTGGAG | 1 cut(s) | 92 |
| BpuEI | CTTGAG | 1 cut(s) | 6 |
| BsaI | GGTCTC | 1 cut(s) | 157 |
| BsaJI | CCNNGG | 1 cut(s) | 48 |
| Bse1I | ACTGG | 1 cut(s) | 109 |
| BseDI | CCNNGG | 1 cut(s) | 48 |
| BseGI | GGATG | 2 cut(s) | 177, 290 |
| BseNI | ACTGG | 1 cut(s) | 109 |
| BsmAI | GTCTC | 1 cut(s) | 157 |
| Bso31I | GGTCTC | 1 cut(s) | 157 |
| BspACI | CCGC | 1 cut(s) | 226 |
| BspTNI | GGTCTC | 1 cut(s) | 157 |
| BsrI | ACTGG | 1 cut(s) | 109 |
| BssECI | CCNNGG | 1 cut(s) | 48 |
| BssT1I | CCWWGG | 1 cut(s) | 48 |
| Bst4CI | ACNGT | 1 cut(s) | 10 |
| BstC8I | GCNNGC | 1 cut(s) | 18 |
| BstF5I | GGATG | 2 cut(s) | 177, 290 |
| BstMAI | GTCTC | 1 cut(s) | 157 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 32, 53, 62 |
| BstNSI | RCATGY | 1 cut(s) | 280 |
| BstV2I | GAAGAC | 1 cut(s) | 138 |
| BtsCI | GGATG | 2 cut(s) | 177, 290 |
| Cac8I | GCNNGC | 1 cut(s) | 18 |
| CviAII | CATG | 1 cut(s) | 277 |
| CviJI | RGCY | 6 cut(s) | 20, 26, 47, 71, 145, 222 |
| CviKI_1 | RGCY | 6 cut(s) | 20, 26, 47, 71, 145, 222 |
| Eco130I | CCWWGG | 1 cut(s) | 48 |
| Eco31I | GGTCTC | 1 cut(s) | 157 |
| EcoT14I | CCWWGG | 1 cut(s) | 48 |
| ErhI | CCWWGG | 1 cut(s) | 48 |
| FaeI | CATG | 1 cut(s) | 280 |
| FaiI | YATR | 8 cut(s) | 93, 95, 114, 120, 122, 219, 278, 307 |
| FatI | CATG | 1 cut(s) | 276 |
| FauNDI | CATATG | 1 cut(s) | 93 |
| FokI | GGATG | 2 cut(s) | 164, 277 |
| FspBI | CTAG | 1 cut(s) | 203 |
| GsuI | CTGGAG | 1 cut(s) | 92 |
| Hin1II | CATG | 1 cut(s) | 280 |
| HindIII | AAGCTT | 1 cut(s) | 24 |
| HinfI | GANTC | 2 cut(s) | 85, 185 |
| HpyAV | CCTTC | 1 cut(s) | 135 |
| HpyCH4III | ACNGT | 1 cut(s) | 10 |
| HpyCH4V | TGCA | 1 cut(s) | 217 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 32, 53, 62 |
| Hsp92II | CATG | 1 cut(s) | 280 |
| LmnI | GCTCC | 1 cut(s) | 111 |
| LpnPI | CCDG | 3 cut(s) | 122, 151, 203 |
| LweI | GCATC | 1 cut(s) | 299 |
| MaeI | CTAG | 1 cut(s) | 203 |
| MaeIII | GTNAC | 1 cut(s) | 182 |
| MboII | GAAGA | 3 cut(s) | 140, 143, 173 |
| MluCI | AATT | 1 cut(s) | 100 |
| MlyI | GAGTC | 2 cut(s) | 79, 179 |
| MnlI | CCTC | 2 cut(s) | 46, 233 |
| MseI | TTAA | 1 cut(s) | 299 |
| MslI | CAYNNNNRTG | 1 cut(s) | 80 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 32, 53, 62 |
| NdeI | CATATG | 1 cut(s) | 93 |
| NlaIII | CATG | 1 cut(s) | 280 |
| NmuCI | GTSAC | 1 cut(s) | 182 |
| NspI | RCATGY | 1 cut(s) | 280 |
| PleI | GAGTC | 2 cut(s) | 79, 179 |
| PpsI | GAGTC | 2 cut(s) | 79, 179 |
| RseI | CAYNNNNRTG | 1 cut(s) | 80 |
| SaqAI | TTAA | 1 cut(s) | 299 |
| SchI | GAGTC | 2 cut(s) | 79, 179 |
| SetI | ASST | 2 cut(s) | 22, 28 |
| SfaNI | GCATC | 1 cut(s) | 299 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 80 |
| SmlI | CTYRAG | 1 cut(s) | 21 |
| SmoI | CTYRAG | 1 cut(s) | 21 |
| Sse9I | AATT | 1 cut(s) | 100 |
| SsiI | CCGC | 1 cut(s) | 226 |
| SspMI | CTAG | 1 cut(s) | 203 |
| StyI | CCWWGG | 1 cut(s) | 48 |
| TaaI | ACNGT | 1 cut(s) | 10 |
| TaqI | TCGA | 1 cut(s) | 159 |
| TaqII | GACCGA | 1 cut(s) | 139 |
| TasI | AATT | 1 cut(s) | 100 |
| Tru1I | TTAA | 1 cut(s) | 299 |
| Tru9I | TTAA | 1 cut(s) | 299 |
| TseFI | GTSAC | 1 cut(s) | 182 |
| Tsp45I | GTSAC | 1 cut(s) | 182 |
| TspDTI | ATGAA | 2 cut(s) | 137, 141 |
| TspGWI | ACGGA | 1 cut(s) | 170 |
| XceI | RCATGY | 1 cut(s) | 280 |
| XspI | CTAG | 1 cut(s) | 203 |