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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 282 |
| AciI | CCGC | 1 cut(s) | 250 |
| AcsI | RAATTY | 1 cut(s) | 317 |
| AfaI | GTAC | 1 cut(s) | 137 |
| AgsI | TTSAA | 1 cut(s) | 265 |
| AjnI | CCWGG | 1 cut(s) | 18 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 177, 209 |
| Alw26I | GTCTC | 1 cut(s) | 181 |
| ApeKI | GCWGC | 2 cut(s) | 68, 169 |
| ApoI | RAATTY | 1 cut(s) | 317 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 294 |
| BbsI | GAAGAC | 1 cut(s) | 162 |
| BbvI | GCAGC | 2 cut(s) | 80, 156 |
| BccI | CCATC | 2 cut(s) | 104, 231 |
| BciT130I | CCWGG | 1 cut(s) | 20 |
| BcoDI | GTCTC | 1 cut(s) | 181 |
| BfaI | CTAG | 1 cut(s) | 15 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 77 |
| BisI | GCNGC | 2 cut(s) | 69, 170 |
| BlsI | GCNGC | 2 cut(s) | 70, 171 |
| Bme1390I | CCNGG | 1 cut(s) | 20 |
| BmrFI | CCNGG | 1 cut(s) | 20 |
| BpiI | GAAGAC | 1 cut(s) | 162 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 165, 197 |
| BpmI | CTGGAG | 1 cut(s) | 116 |
| BpuEI | CTTGAG | 1 cut(s) | 29 |
| Bse1I | ACTGG | 1 cut(s) | 133 |
| BseBI | CCWGG | 1 cut(s) | 20 |
| BseGI | GGATG | 2 cut(s) | 99, 127 |
| BseNI | ACTGG | 1 cut(s) | 133 |
| BseXI | GCAGC | 2 cut(s) | 80, 156 |
| BsmAI | GTCTC | 1 cut(s) | 181 |
| BspACI | CCGC | 1 cut(s) | 250 |
| BsrI | ACTGG | 1 cut(s) | 133 |
| Bst2UI | CCWGG | 1 cut(s) | 20 |
| Bst4CI | ACNGT | 1 cut(s) | 34 |
| BstDEI | CTNAG | 1 cut(s) | 73 |
| BstF5I | GGATG | 2 cut(s) | 99, 127 |
| BstMAI | GTCTC | 1 cut(s) | 181 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 65, 77, 86 |
| BstNI | CCWGG | 1 cut(s) | 20 |
| BstNSI | RCATGY | 1 cut(s) | 28 |
| BstSCI | CCNGG | 1 cut(s) | 18 |
| BstV1I | GCAGC | 2 cut(s) | 80, 156 |
| BstV2I | GAAGAC | 1 cut(s) | 162 |
| BtsCI | GGATG | 2 cut(s) | 99, 127 |
| Csp6I | GTAC | 1 cut(s) | 136 |
| CviAII | CATG | 1 cut(s) | 25 |
| CviJI | RGCY | 6 cut(s) | 13, 18, 71, 95, 169, 246 |
| CviKI_1 | RGCY | 6 cut(s) | 13, 18, 71, 95, 169, 246 |
| CviQI | GTAC | 1 cut(s) | 136 |
| DdeI | CTNAG | 1 cut(s) | 73 |
| EcoRII | CCWGG | 1 cut(s) | 18 |
| FaeI | CATG | 1 cut(s) | 28 |
| FaiI | YATR | 7 cut(s) | 26, 117, 119, 144, 146, 243, 282 |
| FatI | CATG | 1 cut(s) | 24 |
| FauNDI | CATATG | 1 cut(s) | 117 |
| Fnu4HI | GCNGC | 2 cut(s) | 69, 170 |
| FokI | GGATG | 2 cut(s) | 86, 134 |
| Fsp4HI | GCNGC | 2 cut(s) | 69, 170 |
| FspBI | CTAG | 1 cut(s) | 15 |
| GluI | GCNGC | 2 cut(s) | 69, 170 |
| GsuI | CTGGAG | 1 cut(s) | 116 |
| Hin1II | CATG | 1 cut(s) | 28 |
| HinfI | GANTC | 5 cut(s) | 4, 109, 125, 174, 209 |
| Hpy188I | TCNGA | 1 cut(s) | 289 |
| Hpy188III | TCNNGA | 2 cut(s) | 8, 275 |
| HpyAV | CCTTC | 1 cut(s) | 159 |
| HpyCH4III | ACNGT | 1 cut(s) | 34 |
| HpyCH4IV | ACGT | 1 cut(s) | 201 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 65, 77, 86 |
| HpyF3I | CTNAG | 1 cut(s) | 73 |
| HpySE526I | ACGT | 1 cut(s) | 201 |
| Hsp92II | CATG | 1 cut(s) | 28 |
| LmnI | GCTCC | 1 cut(s) | 135 |
| LpnPI | CCDG | 4 cut(s) | 5, 32, 146, 175 |
| Lsp1109I | GCAGC | 2 cut(s) | 80, 156 |
| MaeI | CTAG | 1 cut(s) | 15 |
| MaeII | ACGT | 1 cut(s) | 201 |
| MaeIII | GTNAC | 1 cut(s) | 206 |
| MboII | GAAGA | 3 cut(s) | 164, 167, 197 |
| MluCI | AATT | 1 cut(s) | 317 |
| MlyI | GAGTC | 3 cut(s) | 103, 183, 203 |
| MnlI | CCTC | 2 cut(s) | 70, 257 |
| MroXI | GAANNNNTTC | 1 cut(s) | 294 |
| MseI | TTAA | 2 cut(s) | 303, 311 |
| MslI | CAYNNNNRTG | 2 cut(s) | 104, 120 |
| MspR9I | CCNGG | 1 cut(s) | 20 |
| MvaI | CCWGG | 1 cut(s) | 20 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 65, 77, 86 |
| NdeI | CATATG | 1 cut(s) | 117 |
| NlaIII | CATG | 1 cut(s) | 28 |
| NmuCI | GTSAC | 1 cut(s) | 206 |
| NspI | RCATGY | 1 cut(s) | 28 |
| PdmI | GAANNNNTTC | 1 cut(s) | 294 |
| PfeI | GAWTC | 2 cut(s) | 4, 125 |
| PkrI | GCNGC | 2 cut(s) | 70, 171 |
| PleI | GAGTC | 3 cut(s) | 103, 182, 203 |
| PpsI | GAGTC | 3 cut(s) | 103, 182, 203 |
| PsiI | TTATAA | 1 cut(s) | 282 |
| Psp6I | CCWGG | 1 cut(s) | 18 |
| PspGI | CCWGG | 1 cut(s) | 18 |
| RsaI | GTAC | 1 cut(s) | 137 |
| RsaNI | GTAC | 1 cut(s) | 136 |
| RseI | CAYNNNNRTG | 2 cut(s) | 104, 120 |
| SaqAI | TTAA | 2 cut(s) | 303, 311 |
| SatI | GCNGC | 2 cut(s) | 69, 170 |
| SchI | GAGTC | 3 cut(s) | 103, 183, 203 |
| ScrFI | CCNGG | 1 cut(s) | 20 |
| SetI | ASST | 2 cut(s) | 204, 228 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 104, 120 |
| SmlI | CTYRAG | 1 cut(s) | 8 |
| SmoI | CTYRAG | 1 cut(s) | 8 |
| Sse9I | AATT | 1 cut(s) | 317 |
| SsiI | CCGC | 1 cut(s) | 250 |
| SspMI | CTAG | 1 cut(s) | 15 |
| StyD4I | CCNGG | 1 cut(s) | 18 |
| TaaI | ACNGT | 1 cut(s) | 34 |
| TaiI | ACGT | 1 cut(s) | 204 |
| TaqI | TCGA | 1 cut(s) | 183 |
| TasI | AATT | 1 cut(s) | 317 |
| TfiI | GAWTC | 2 cut(s) | 4, 125 |
| Tru1I | TTAA | 2 cut(s) | 303, 311 |
| Tru9I | TTAA | 2 cut(s) | 303, 311 |
| TseFI | GTSAC | 1 cut(s) | 206 |
| TseI | GCWGC | 2 cut(s) | 68, 169 |
| Tsp45I | GTSAC | 1 cut(s) | 206 |
| TspDTI | ATGAA | 1 cut(s) | 161 |
| TspGWI | ACGGA | 1 cut(s) | 194 |
| XapI | RAATTY | 1 cut(s) | 317 |
| XceI | RCATGY | 1 cut(s) | 28 |
| XmnI | GAANNNNTTC | 1 cut(s) | 294 |
| XspI | CTAG | 1 cut(s) | 15 |