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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 236 |
| AcsI | RAATTY | 2 cut(s) | 177, 291 |
| AfaI | GTAC | 1 cut(s) | 123 |
| AgsI | TTSAA | 1 cut(s) | 251 |
| AjiI | CACGTC | 1 cut(s) | 11 |
| AjnI | CCWGG | 1 cut(s) | 4 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 163, 195 |
| AluBI | AGCT | 3 cut(s) | 30, 36, 81 |
| AluI | AGCT | 3 cut(s) | 30, 36, 81 |
| Alw26I | GTCTC | 1 cut(s) | 167 |
| ApeKI | GCWGC | 1 cut(s) | 54 |
| ApoI | RAATTY | 2 cut(s) | 177, 291 |
| BbsI | GAAGAC | 1 cut(s) | 148 |
| BbvI | GCAGC | 1 cut(s) | 66 |
| BccI | CCATC | 1 cut(s) | 217 |
| BciT130I | CCWGG | 1 cut(s) | 6 |
| BcoDI | GTCTC | 1 cut(s) | 167 |
| BfaI | CTAG | 1 cut(s) | 213 |
| BfmI | CTRYAG | 1 cut(s) | 19 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 63 |
| BisI | GCNGC | 1 cut(s) | 55 |
| BlsI | GCNGC | 1 cut(s) | 56 |
| Bme1390I | CCNGG | 1 cut(s) | 6 |
| BmgBI | CACGTC | 1 cut(s) | 11 |
| BmrFI | CCNGG | 1 cut(s) | 6 |
| BpiI | GAAGAC | 1 cut(s) | 148 |
| BpmI | CTGGAG | 1 cut(s) | 102 |
| BpuEI | CTTGAG | 1 cut(s) | 16 |
| BsaI | GGTCTC | 1 cut(s) | 167 |
| Bse1I | ACTGG | 1 cut(s) | 119 |
| BseBI | CCWGG | 1 cut(s) | 6 |
| BseGI | GGATG | 3 cut(s) | 85, 113, 277 |
| BseNI | ACTGG | 1 cut(s) | 119 |
| BseXI | GCAGC | 1 cut(s) | 66 |
| BsmAI | GTCTC | 1 cut(s) | 167 |
| Bso31I | GGTCTC | 1 cut(s) | 167 |
| BspACI | CCGC | 1 cut(s) | 236 |
| BspTNI | GGTCTC | 1 cut(s) | 167 |
| BsrI | ACTGG | 1 cut(s) | 119 |
| Bst2UI | CCWGG | 1 cut(s) | 6 |
| Bst4CI | ACNGT | 1 cut(s) | 20 |
| BstC8I | GCNNGC | 1 cut(s) | 28 |
| BstDEI | CTNAG | 1 cut(s) | 59 |
| BstF5I | GGATG | 3 cut(s) | 85, 113, 277 |
| BstMAI | GTCTC | 1 cut(s) | 167 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 42, 51, 63, 72 |
| BstNI | CCWGG | 1 cut(s) | 6 |
| BstSCI | CCNGG | 1 cut(s) | 4 |
| BstSFI | CTRYAG | 1 cut(s) | 19 |
| BstV1I | GCAGC | 1 cut(s) | 66 |
| BstV2I | GAAGAC | 1 cut(s) | 148 |
| BtrI | CACGTC | 1 cut(s) | 11 |
| BtsCI | GGATG | 3 cut(s) | 85, 113, 277 |
| Cac8I | GCNNGC | 1 cut(s) | 28 |
| Csp6I | GTAC | 1 cut(s) | 122 |
| CviJI | RGCY | 7 cut(s) | 4, 30, 36, 57, 81, 155, 232 |
| CviKI_1 | RGCY | 7 cut(s) | 4, 30, 36, 57, 81, 155, 232 |
| CviQI | GTAC | 1 cut(s) | 122 |
| DdeI | CTNAG | 1 cut(s) | 59 |
| Eco31I | GGTCTC | 1 cut(s) | 167 |
| EcoRII | CCWGG | 1 cut(s) | 4 |
| FaiI | YATR | 6 cut(s) | 103, 105, 130, 132, 229, 268 |
| FauNDI | CATATG | 1 cut(s) | 103 |
| Fnu4HI | GCNGC | 1 cut(s) | 55 |
| FokI | GGATG | 3 cut(s) | 72, 120, 264 |
| Fsp4HI | GCNGC | 1 cut(s) | 55 |
| FspBI | CTAG | 1 cut(s) | 213 |
| GluI | GCNGC | 1 cut(s) | 55 |
| GsuI | CTGGAG | 1 cut(s) | 102 |
| HindIII | AAGCTT | 1 cut(s) | 34 |
| HinfI | GANTC | 3 cut(s) | 95, 111, 195 |
| Hpy188III | TCNNGA | 3 cut(s) | 207, 261, 281 |
| HpyAV | CCTTC | 1 cut(s) | 145 |
| HpyCH4III | ACNGT | 1 cut(s) | 20 |
| HpyCH4IV | ACGT | 2 cut(s) | 10, 187 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 42, 51, 63, 72 |
| HpyF3I | CTNAG | 1 cut(s) | 59 |
| HpySE526I | ACGT | 2 cut(s) | 10, 187 |
| LmnI | GCTCC | 1 cut(s) | 121 |
| LpnPI | CCDG | 5 cut(s) | 18, 132, 161, 220, 301 |
| Lsp1109I | GCAGC | 1 cut(s) | 66 |
| MaeI | CTAG | 1 cut(s) | 213 |
| MaeII | ACGT | 2 cut(s) | 10, 187 |
| MaeIII | GTNAC | 1 cut(s) | 192 |
| MboII | GAAGA | 2 cut(s) | 153, 183 |
| MluCI | AATT | 2 cut(s) | 177, 291 |
| MlyI | GAGTC | 2 cut(s) | 89, 189 |
| MnlI | CCTC | 2 cut(s) | 56, 243 |
| MslI | CAYNNNNRTG | 2 cut(s) | 90, 106 |
| MspR9I | CCNGG | 1 cut(s) | 6 |
| MvaI | CCWGG | 1 cut(s) | 6 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 42, 51, 63, 72 |
| NdeI | CATATG | 1 cut(s) | 103 |
| NmuCI | GTSAC | 1 cut(s) | 192 |
| PfeI | GAWTC | 1 cut(s) | 111 |
| PkrI | GCNGC | 1 cut(s) | 56 |
| PleI | GAGTC | 2 cut(s) | 89, 189 |
| PpsI | GAGTC | 2 cut(s) | 89, 189 |
| Psp6I | CCWGG | 1 cut(s) | 4 |
| PspGI | CCWGG | 1 cut(s) | 4 |
| RsaI | GTAC | 1 cut(s) | 123 |
| RsaNI | GTAC | 1 cut(s) | 122 |
| RseI | CAYNNNNRTG | 2 cut(s) | 90, 106 |
| SatI | GCNGC | 1 cut(s) | 55 |
| SchI | GAGTC | 2 cut(s) | 89, 189 |
| ScrFI | CCNGG | 1 cut(s) | 6 |
| SetI | ASST | 6 cut(s) | 13, 32, 38, 83, 190, 214 |
| SfcI | CTRYAG | 1 cut(s) | 19 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 90, 106 |
| SmlI | CTYRAG | 1 cut(s) | 31 |
| SmoI | CTYRAG | 1 cut(s) | 31 |
| Sse9I | AATT | 2 cut(s) | 177, 291 |
| SsiI | CCGC | 1 cut(s) | 236 |
| SspMI | CTAG | 1 cut(s) | 213 |
| StyD4I | CCNGG | 1 cut(s) | 4 |
| TaaI | ACNGT | 1 cut(s) | 20 |
| TaiI | ACGT | 2 cut(s) | 13, 190 |
| TaqI | TCGA | 1 cut(s) | 169 |
| TasI | AATT | 2 cut(s) | 177, 291 |
| TatI | WGTACW | 1 cut(s) | 121 |
| TfiI | GAWTC | 1 cut(s) | 111 |
| TseFI | GTSAC | 1 cut(s) | 192 |
| TseI | GCWGC | 1 cut(s) | 54 |
| Tsp45I | GTSAC | 1 cut(s) | 192 |
| TspDTI | ATGAA | 1 cut(s) | 147 |
| TspGWI | ACGGA | 1 cut(s) | 180 |
| XapI | RAATTY | 2 cut(s) | 177, 291 |
| XspI | CTAG | 1 cut(s) | 213 |