Rmu_sc0001251.1_g000002

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001251.1
OG0004305
Physical Location & Seq
Forward (+)
8218 .. 9068
851 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 528 bp
atgtctgcgtcccattaccactggtacacatcggagttcgccgacccacctcaaatcctccacgtatacgataccgttttccatggcttctcagcaactctcacctccgaccaagttgcctccctcagccaacaccctgccgttctccacgacttcgaagaccgccgccgccagctccacaccacccgcttgcagcaaatttggccacagcgtcggagcttttccgataggaatctcagtccggttccgaagcggtggagaaaaattgggcctcgacgaagaagcttatacagtcaatcgattccggtcgacgcgcttcctgggtccggtagccggattctggattccggtcactggtcgccggattccagtcatcagtcgccagtcgctgcagtccgcggccagccactggtcaccggtctggtagccggattcgggattccggtcactggtcgccggattaggtcattaatcgccagtcgctggagtccgcggccggccactggtcaccggagcatagcaaggtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

175

Amino Acids

19.73

Weight (kDa)

11.51

Isoelectric Point (pI)

89.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 4 - 60 6.8e-11 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 409, 483
AccI GTMKAC 2 cut(s) 66, 309
AccII CGCG 3 cut(s) 314, 399, 493
AciI CCGC 9 cut(s) 163, 166, 169, 187, 253, 397, 399, 491, 493
AcoI YGGCCR 4 cut(s) 203, 400, 494, 498
AcsI RAATTY 1 cut(s) 198
AfaI GTAC 1 cut(s) 26
AfiI CCNNNNNNNGG 9 cut(s) 326, 333, 340, 354, 409, 435, 449, 483, 503
AgeI ACCGGT 1 cut(s) 416
AjnI CCWGG 1 cut(s) 319
AluBI AGCT 3 cut(s) 175, 219, 285
AluI AGCT 3 cut(s) 175, 219, 285
AlwNI CAGNNNCTG 3 cut(s) 389, 409, 483
AoxI GGCC 5 cut(s) 203, 269, 400, 494, 498
ApeKI GCWGC 2 cut(s) 193, 389
ApoI RAATTY 1 cut(s) 198
AseI ATTAAT 1 cut(s) 470
AsiGI ACCGGT 1 cut(s) 416
AspLEI GCGC 1 cut(s) 316
AspS9I GGNCC 2 cut(s) 269, 324
AsuHPI GGTGA 3 cut(s) 94, 406, 500
AsuII TTCGAA 1 cut(s) 156
AvaII GGWCC 1 cut(s) 324
BalI TGGCCA 1 cut(s) 205
BbsI GAAGAC 1 cut(s) 165
BbvCI CCTCAGC 1 cut(s) 125
BbvI GCAGC 2 cut(s) 205, 376
BceAI ACGGC 1 cut(s) 125
BcgI CGANNNNNNTGC 2 cut(s) 98, 132
BciT130I CCWGG 1 cut(s) 321
BfmI CTRYAG 1 cut(s) 390
BisI GCNGC 6 cut(s) 166, 169, 194, 390, 400, 494
BlsI GCNGC 6 cut(s) 167, 170, 195, 391, 401, 495
Bme1390I CCNGG 1 cut(s) 321
Bme18I GGWCC 1 cut(s) 324
BmgT120I GGNCC 2 cut(s) 269, 324
BmiI GGNNCC 2 cut(s) 246, 325
BmrFI CCNGG 1 cut(s) 321
BpiI GAAGAC 1 cut(s) 165
BpmI CTGGAG 1 cut(s) 505
Bpu10I CCTNAGC 1 cut(s) 125
Bpu14I TTCGAA 1 cut(s) 156
Bsa29I ATCGAT 1 cut(s) 299
BsaAI YACGTR 1 cut(s) 64
BsaBI GATNNNNATC 1 cut(s) 231
BsaJI CCNNGG 4 cut(s) 82, 320, 397, 491
BsaWI WCCGGW 7 cut(s) 241, 304, 326, 347, 416, 442, 510
Bsc4I CCNNNNNNNGG 9 cut(s) 326, 333, 340, 354, 409, 435, 449, 483, 503
Bse118I RCCGGY 2 cut(s) 416, 496
Bse1I ACTGG 8 cut(s) 26, 359, 369, 383, 414, 454, 477, 508
Bse8I GATNNNNATC 1 cut(s) 231
BseBI CCWGG 1 cut(s) 321
BseCI ATCGAT 1 cut(s) 299
BseDI CCNNGG 4 cut(s) 82, 320, 397, 491
BseJI GATNNNNATC 1 cut(s) 231
BseLI CCNNNNNNNGG 9 cut(s) 326, 333, 340, 354, 409, 435, 449, 483, 503
BseMII CTCAG 3 cut(s) 105, 139, 250
BseNI ACTGG 8 cut(s) 26, 359, 369, 383, 414, 454, 477, 508
BseX3I CGGCCG 1 cut(s) 494
BseXI GCAGC 2 cut(s) 205, 376
Bsh1236I CGCG 3 cut(s) 314, 399, 493
Bsh1285I CGRYCG 2 cut(s) 309, 497
BshFI GGCC 5 cut(s) 205, 271, 402, 496, 500
BshTI ACCGGT 1 cut(s) 416
BshVI ATCGAT 1 cut(s) 299
BsiEI CGRYCG 2 cut(s) 309, 497
BslI CCNNNNNNNGG 9 cut(s) 326, 333, 340, 354, 409, 435, 449, 483, 503
BsnI GGCC 5 cut(s) 205, 271, 402, 496, 500
Bsp119I TTCGAA 1 cut(s) 156
Bsp19I CCATGG 1 cut(s) 82
BspACI CCGC 9 cut(s) 163, 166, 169, 187, 253, 397, 399, 491, 493
BspANI GGCC 5 cut(s) 205, 271, 402, 496, 500
BspCNI CTCAG 3 cut(s) 104, 138, 249
BspDI ATCGAT 1 cut(s) 299
BspFNI CGCG 3 cut(s) 314, 399, 493
BspLI GGNNCC 2 cut(s) 246, 325
BspMAI CTGCAG 1 cut(s) 394
BspT104I TTCGAA 1 cut(s) 156
BsrFI RCCGGY 2 cut(s) 416, 496
BsrI ACTGG 8 cut(s) 26, 359, 369, 383, 414, 454, 477, 508
BssAI RCCGGY 2 cut(s) 416, 496
BssECI CCNNGG 4 cut(s) 82, 320, 397, 491
BssNAI GTATAC 1 cut(s) 67
BssT1I CCWWGG 1 cut(s) 82
Bst1107I GTATAC 1 cut(s) 67
Bst2UI CCWGG 1 cut(s) 321
Bst4CI ACNGT 2 cut(s) 76, 293
BstBAI YACGTR 1 cut(s) 64
BstBI TTCGAA 1 cut(s) 156
BstC8I GCNNGC 4 cut(s) 173, 191, 404, 498
BstDEI CTNAG 3 cut(s) 91, 125, 236
BstDSI CCRYGG 3 cut(s) 82, 397, 491
BstEII GGTNACC 2 cut(s) 412, 506
BstFNI CGCG 3 cut(s) 314, 399, 493
BstHHI GCGC 1 cut(s) 316
BstMCI CGRYCG 2 cut(s) 309, 497
BstMWI GCNNNNNNNGC 1 cut(s) 202
BstNI CCWGG 1 cut(s) 321
BstPI GGTNACC 2 cut(s) 412, 506
BstSCI CCNGG 1 cut(s) 319
BstSFI CTRYAG 1 cut(s) 390
BstUI CGCG 3 cut(s) 314, 399, 493
BstV1I GCAGC 2 cut(s) 205, 376
BstV2I GAAGAC 1 cut(s) 165
BstZ17I GTATAC 1 cut(s) 67
BstZI CGGCCG 1 cut(s) 494
Bsu15I ATCGAT 1 cut(s) 299
BsuRI GGCC 5 cut(s) 205, 271, 402, 496, 500
BsuTUI ATCGAT 1 cut(s) 299
BtgI CCRYGG 3 cut(s) 82, 397, 491
BtsIMutI CAGTG 5 cut(s) 19, 352, 407, 447, 501
Cac8I GCNNGC 4 cut(s) 173, 191, 404, 498
CaiI CAGNNNCTG 3 cut(s) 389, 409, 483
CfoI GCGC 1 cut(s) 316
Cfr10I RCCGGY 2 cut(s) 416, 496
Cfr13I GGNCC 2 cut(s) 269, 324
Cfr42I CCGCGG 2 cut(s) 400, 494
ClaI ATCGAT 1 cut(s) 299
CseI GACGC 2 cut(s) 200, 320
Csp6I GTAC 1 cut(s) 25
CspAI ACCGGT 1 cut(s) 416
CviAII CATG 1 cut(s) 83
CviQI GTAC 1 cut(s) 25
DdeI CTNAG 3 cut(s) 91, 125, 236
EaeI YGGCCR 4 cut(s) 203, 400, 494, 498
EagI CGGCCG 1 cut(s) 494
EclXI CGGCCG 1 cut(s) 494
Eco130I CCWWGG 1 cut(s) 82
Eco47I GGWCC 1 cut(s) 324
Eco52I CGGCCG 1 cut(s) 494
Eco91I GGTNACC 2 cut(s) 412, 506
EcoO65I GGTNACC 2 cut(s) 412, 506
EcoRII CCWGG 1 cut(s) 319
EcoT14I CCWWGG 1 cut(s) 82
ErhI CCWWGG 1 cut(s) 82
FaeI CATG 1 cut(s) 86
FaiI YATR 4 cut(s) 67, 84, 289, 518
FatI CATG 1 cut(s) 82
FauI CCCGC 1 cut(s) 194
FblI GTMKAC 2 cut(s) 66, 309
Fnu4HI GCNGC 6 cut(s) 166, 169, 194, 390, 400, 494
FseI GGCCGGCC 1 cut(s) 500
Fsp4HI GCNGC 6 cut(s) 166, 169, 194, 390, 400, 494
GlaI GCGC 1 cut(s) 315
GluI GCNGC 6 cut(s) 166, 169, 194, 390, 400, 494
GsuI CTGGAG 1 cut(s) 505
HaeIII GGCC 5 cut(s) 205, 271, 402, 496, 500
HgaI GACGC 2 cut(s) 200, 320
HhaI GCGC 1 cut(s) 316
Hin1II CATG 1 cut(s) 86
Hin6I GCGC 1 cut(s) 314
HinP1I GCGC 1 cut(s) 314
HincII GTYRAC 1 cut(s) 310
HindII GTYRAC 1 cut(s) 310
HindIII AAGCTT 1 cut(s) 283
HinfI GANTC 8 cut(s) 232, 301, 337, 344, 365, 432, 439, 487
HphI GGTGA 3 cut(s) 94, 406, 500
Hpy166II GTNNAC 3 cut(s) 27, 67, 310
Hpy188I TCNGA 5 cut(s) 34, 109, 216, 226, 249
Hpy188III TCNNGA 2 cut(s) 341, 436
Hpy8I GTNNAC 3 cut(s) 27, 67, 310
Hpy99I CGWCG 3 cut(s) 216, 279, 314
HpyCH4III ACNGT 2 cut(s) 76, 293
HpyCH4IV ACGT 1 cut(s) 63
HpyCH4V TGCA 2 cut(s) 193, 392
HpyF10VI GCNNNNNNNGC 1 cut(s) 202
HpyF3I CTNAG 3 cut(s) 91, 125, 236
HpySE526I ACGT 1 cut(s) 63
Hsp92II CATG 1 cut(s) 86
HspAI GCGC 1 cut(s) 314
KroI GCCGGC 1 cut(s) 496
KroNI GCCGGC 1 cut(s) 498
KspI CCGCGG 2 cut(s) 400, 494
LmnI GCTCC 3 cut(s) 180, 216, 513
Lsp1109I GCAGC 2 cut(s) 205, 376
MaeII ACGT 1 cut(s) 63
MaeIII GTNAC 4 cut(s) 350, 412, 445, 506
MboII GAAGA 2 cut(s) 170, 291
MlsI TGGCCA 1 cut(s) 205
MluCI AATT 2 cut(s) 198, 264
MluNI TGGCCA 1 cut(s) 205
MlyI GAGTC 1 cut(s) 496
MmeI TCCRAC 2 cut(s) 132, 194
MnlI CCTC 6 cut(s) 60, 68, 115, 130, 134, 282
Mox20I TGGCCA 1 cut(s) 205
MroNI GCCGGC 1 cut(s) 496
MscI TGGCCA 1 cut(s) 205
MseI TTAA 1 cut(s) 470
Msp20I TGGCCA 1 cut(s) 205
MspA1I CMGCKG 2 cut(s) 399, 493
MspR9I CCNGG 1 cut(s) 321
MvaI CCWGG 1 cut(s) 321
MvnI CGCG 3 cut(s) 314, 399, 493
MwoI GCNNNNNNNGC 1 cut(s) 202
NaeI GCCGGC 1 cut(s) 498
NcoI CCATGG 1 cut(s) 82
NgoMIV GCCGGC 1 cut(s) 496
NlaIII CATG 1 cut(s) 86
NlaIV GGNNCC 2 cut(s) 246, 325
NmuCI GTSAC 4 cut(s) 350, 412, 445, 506
NspV TTCGAA 1 cut(s) 156
PdiI GCCGGC 1 cut(s) 498
PfeI GAWTC 7 cut(s) 232, 301, 337, 344, 365, 432, 439
PflMI CCANNNNNTGG 2 cut(s) 409, 483
PinAI ACCGGT 1 cut(s) 416
PkrI GCNGC 6 cut(s) 167, 170, 195, 391, 401, 495
PleI GAGTC 1 cut(s) 495
PpsI GAGTC 1 cut(s) 495
Ppu21I YACGTR 1 cut(s) 64
PshBI ATTAAT 1 cut(s) 470
Psp6I CCWGG 1 cut(s) 319
PspEI GGTNACC 2 cut(s) 412, 506
PspGI CCWGG 1 cut(s) 319
PspN4I GGNNCC 2 cut(s) 246, 325
PspPI GGNCC 2 cut(s) 269, 324
PstI CTGCAG 1 cut(s) 394
PstNI CAGNNNCTG 3 cut(s) 389, 409, 483
RigI GGCCGGCC 1 cut(s) 500
RsaI GTAC 1 cut(s) 26
RsaNI GTAC 1 cut(s) 25
SacII CCGCGG 2 cut(s) 400, 494
SalI GTCGAC 1 cut(s) 308
SaqAI TTAA 1 cut(s) 470
SatI GCNGC 6 cut(s) 166, 169, 194, 390, 400, 494
Sau96I GGNCC 2 cut(s) 269, 324
SchI GAGTC 1 cut(s) 496
ScrFI CCNGG 1 cut(s) 321
SetI ASST 8 cut(s) 52, 66, 107, 177, 221, 287, 467, 527
SfcI CTRYAG 1 cut(s) 390
Sfr303I CCGCGG 2 cut(s) 400, 494
SfuI TTCGAA 1 cut(s) 156
SgrBI CCGCGG 2 cut(s) 400, 494
SinI GGWCC 1 cut(s) 324
Sse9I AATT 2 cut(s) 198, 264
SsiI CCGC 9 cut(s) 163, 166, 169, 187, 253, 397, 399, 491, 493
StyD4I CCNGG 1 cut(s) 319
StyI CCWWGG 1 cut(s) 82
TaaI ACNGT 2 cut(s) 76, 293
TaiI ACGT 1 cut(s) 66
TaqI TCGA 4 cut(s) 156, 274, 299, 309
TasI AATT 2 cut(s) 198, 264
TauI GCSGC 4 cut(s) 168, 171, 402, 496
TfiI GAWTC 7 cut(s) 232, 301, 337, 344, 365, 432, 439
Tru1I TTAA 1 cut(s) 470
Tru9I TTAA 1 cut(s) 470
TscAI CASTG 5 cut(s) 26, 359, 414, 454, 508
TseFI GTSAC 4 cut(s) 350, 412, 445, 506
TseI GCWGC 2 cut(s) 193, 389
Tsp45I GTSAC 4 cut(s) 350, 412, 445, 506
TspRI CASTG 5 cut(s) 26, 359, 414, 454, 508
Van91I CCANNNNNTGG 2 cut(s) 409, 483
VpaK11BI GGWCC 1 cut(s) 324
VspI ATTAAT 1 cut(s) 470
XapI RAATTY 1 cut(s) 198
XmiI GTMKAC 2 cut(s) 66, 309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.