Rmu_sc0001474.1_g000002

prefoldin subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001474.1
OG0003635
Physical Location & Seq
Reverse (-)
7288 .. 16330
9043 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 660 bp
atgaggtattttgacggatcagttgaaactattagctcgccacctccagcagcagaagcaccagttcccatcactcagaatgaggttgaagacacaactgcccctcctgccatcgcttctacccctgacccacagcttcctggtactgaagatcacccatttgaggtactgttgcaagcttcgattcactgccaaattaccgcacacagagacggaagagtcatgtctagtagtaaagctgaagaacctgtaaatgagcaagcagttgcaaataagtttgctgccatgaggtctgaactcaaccaaatttactctaaaatcactgagctagagatggacgtgagtgagcactcattggtgatcaatgccatccagccactcgacccatccaggcggtgcttccgaatgattggaggtgtgctggtggagagaaccatcaaggaggtcctgcctgctgtgcagcgtaacaaagaagggattgaggaggttattactcggctgaatgaggctttggaaaggaagaaaaaagaaatttctgactttgaggccaagtacaagatccggataagaaagaacgacagtgaggagaaggatgatggtgctcgcaaagaaggaactgctcaaggagtcctggtcggccctgctggtggaagcgaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006457 GO:0008150 GO:0009987 GO:0044183 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prefoldin_2

Protein Analysis

219

Amino Acids

24.12

Weight (kDa)

5.06

Isoelectric Point (pI)

54.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prefoldin_2 PF01920 87 - 191 5.1e-26 Prefoldin subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 218
AccIII TCCGGA 1 cut(s) 561
AciI CCGC 2 cut(s) 201, 394
AclWI GGATC 2 cut(s) 25, 553
AcsI RAATTY 2 cut(s) 306, 531
AcuI CTGAAG 2 cut(s) 168, 261
AfaI GTAC 3 cut(s) 145, 168, 554
AfiI CCNNNNNNNGG 2 cut(s) 163, 647
AgsI TTSAA 2 cut(s) 26, 89
AjiI CACGTC 1 cut(s) 340
AjnI CCWGG 3 cut(s) 139, 389, 630
AjuI GAANNNNNNNTTGG 2 cut(s) 494, 526
AluBI AGCT 5 cut(s) 36, 136, 179, 239, 328
AluI AGCT 5 cut(s) 36, 136, 179, 239, 328
Alw21I GWGCWC 2 cut(s) 351, 604
Alw26I GTCTC 1 cut(s) 204
AlwI GGATC 2 cut(s) 25, 553
Aor13HI TCCGGA 1 cut(s) 561
AoxI GGCC 2 cut(s) 546, 637
ApeKI GCWGC 3 cut(s) 50, 281, 460
ApoI RAATTY 2 cut(s) 306, 531
AspS9I GGNCC 2 cut(s) 445, 638
AsuHPI GGTGA 2 cut(s) 146, 370
AvaII GGWCC 1 cut(s) 445
BbsI GAAGAC 1 cut(s) 96
Bbv12I GWGCWC 2 cut(s) 351, 604
BbvI GCAGC 3 cut(s) 62, 268, 472
BccI CCATC 7 cut(s) 77, 119, 328, 377, 394, 443, 590
BciT130I CCWGG 3 cut(s) 141, 391, 632
BclI TGATCA 1 cut(s) 360
BcoDI GTCTC 1 cut(s) 204
BfaI CTAG 2 cut(s) 228, 329
BisI GCNGC 3 cut(s) 51, 282, 461
BlsI GCNGC 3 cut(s) 52, 283, 462
Bme1390I CCNGG 3 cut(s) 141, 391, 632
Bme18I GGWCC 1 cut(s) 445
BmgBI CACGTC 1 cut(s) 340
BmgT120I GGNCC 2 cut(s) 445, 638
BmrFI CCNGG 3 cut(s) 141, 391, 632
BpiI GAAGAC 1 cut(s) 96
BpmI CTGGAG 1 cut(s) 30
BpuEI CTTGAG 1 cut(s) 606
BsaWI WCCGGW 1 cut(s) 561
BsaXI ACNNNNNCTCC 4 cut(s) 88, 118, 618, 648
Bsc4I CCNNNNNNNGG 2 cut(s) 163, 647
Bse1I ACTGG 1 cut(s) 62
BseAI TCCGGA 1 cut(s) 561
BseBI CCWGG 3 cut(s) 141, 391, 632
BseGI GGATG 3 cut(s) 369, 386, 598
BseLI CCNNNNNNNGG 2 cut(s) 163, 647
BseMII CTCAG 2 cut(s) 89, 315
BseNI ACTGG 1 cut(s) 62
BseRI GAGGAG 2 cut(s) 497, 599
BseXI GCAGC 3 cut(s) 62, 268, 472
BsgI GTGCAG 1 cut(s) 479
BshFI GGCC 2 cut(s) 548, 639
BsiHKAI GWGCWC 2 cut(s) 351, 604
BsiSI CCGG 1 cut(s) 562
BslI CCNNNNNNNGG 2 cut(s) 163, 647
BsmAI GTCTC 1 cut(s) 204
BsmBI CGTCTC 1 cut(s) 204
BsnI GGCC 2 cut(s) 548, 639
Bsp1286I GDGCHC 2 cut(s) 351, 604
Bsp13I TCCGGA 1 cut(s) 561
Bsp143I GATC 4 cut(s) 17, 151, 360, 558
BspACI CCGC 2 cut(s) 201, 394
BspANI GGCC 2 cut(s) 548, 639
BspCNI CTCAG 2 cut(s) 88, 316
BspEI TCCGGA 1 cut(s) 561
BspPI GGATC 2 cut(s) 25, 553
BsrI ACTGG 1 cut(s) 62
BssMI GATC 4 cut(s) 17, 151, 360, 558
Bst2UI CCWGG 3 cut(s) 141, 391, 632
Bst4CI ACNGT 2 cut(s) 171, 581
Bst6I CTCTTC 1 cut(s) 211
BstC8I GCNNGC 5 cut(s) 38, 177, 261, 453, 604
BstDEI CTNAG 2 cut(s) 75, 324
BstF5I GGATG 3 cut(s) 369, 386, 598
BstKTI GATC 4 cut(s) 20, 154, 363, 561
BstMAI GTCTC 1 cut(s) 204
BstMBI GATC 4 cut(s) 17, 151, 360, 558
BstMWI GCNNNNNNNGC 3 cut(s) 56, 107, 457
BstNI CCWGG 3 cut(s) 141, 391, 632
BstSCI CCNGG 3 cut(s) 139, 389, 630
BstV1I GCAGC 3 cut(s) 62, 268, 472
BstV2I GAAGAC 1 cut(s) 96
BstX2I RGATCY 1 cut(s) 558
BstYI RGATCY 1 cut(s) 558
BsuRI GGCC 2 cut(s) 548, 639
BtgZI GCGATG 1 cut(s) 97
BtrI CACGTC 1 cut(s) 340
BtsCI GGATG 3 cut(s) 369, 386, 598
BtsI GCAGTG 1 cut(s) 187
BtsIMutI CAGTG 3 cut(s) 187, 321, 586
Cac8I GCNNGC 5 cut(s) 38, 177, 261, 453, 604
Cfr13I GGNCC 2 cut(s) 445, 638
Csp6I GTAC 3 cut(s) 144, 167, 553
CviAII CATG 2 cut(s) 223, 286
CviQI GTAC 3 cut(s) 144, 167, 553
DdeI CTNAG 2 cut(s) 75, 324
DpnI GATC 4 cut(s) 19, 153, 362, 560
DpnII GATC 4 cut(s) 17, 151, 360, 558
DrdI GACNNNNNNGTC 1 cut(s) 218
DseDI GACNNNNNNGTC 1 cut(s) 218
Eam1104I CTCTTC 1 cut(s) 211
EarI CTCTTC 1 cut(s) 211
Eco47I GGWCC 1 cut(s) 445
Eco57I CTGAAG 2 cut(s) 168, 261
EcoO109I RGGNCCY 1 cut(s) 445
EcoRII CCWGG 3 cut(s) 139, 389, 630
Esp3I CGTCTC 1 cut(s) 204
FaeI CATG 2 cut(s) 226, 289
FaiI YATR 2 cut(s) 224, 287
FatI CATG 2 cut(s) 222, 285
FbaI TGATCA 1 cut(s) 360
Fnu4HI GCNGC 3 cut(s) 51, 282, 461
FokI GGATG 3 cut(s) 356, 373, 605
Fsp4HI GCNGC 3 cut(s) 51, 282, 461
FspBI CTAG 2 cut(s) 228, 329
GluI GCNGC 3 cut(s) 51, 282, 461
GsuI CTGGAG 1 cut(s) 30
HaeIII GGCC 2 cut(s) 548, 639
HapII CCGG 1 cut(s) 562
Hin1II CATG 2 cut(s) 226, 289
HindIII AAGCTT 1 cut(s) 177
HinfI GANTC 3 cut(s) 184, 219, 627
HpaII CCGG 1 cut(s) 562
HphI GGTGA 2 cut(s) 146, 370
Hpy188I TCNGA 4 cut(s) 78, 295, 404, 538
Hpy188III TCNNGA 1 cut(s) 562
HpyAV CCTTC 3 cut(s) 467, 583, 605
HpyCH4III ACNGT 2 cut(s) 171, 581
HpyCH4IV ACGT 1 cut(s) 339
HpyCH4V TGCA 3 cut(s) 175, 269, 460
HpyF10VI GCNNNNNNNGC 3 cut(s) 56, 107, 457
HpyF3I CTNAG 2 cut(s) 75, 324
HpySE526I ACGT 1 cut(s) 339
Hsp92II CATG 2 cut(s) 226, 289
Kpn2I TCCGGA 1 cut(s) 561
Ksp22I TGATCA 1 cut(s) 360
Kzo9I GATC 4 cut(s) 17, 151, 360, 558
Lsp1109I GCAGC 3 cut(s) 62, 268, 472
MaeI CTAG 2 cut(s) 228, 329
MaeII ACGT 1 cut(s) 339
MaeIII GTNAC 1 cut(s) 464
MalI GATC 4 cut(s) 19, 153, 362, 560
MboI GATC 4 cut(s) 17, 151, 360, 558
MboII GAAGA 5 cut(s) 101, 161, 228, 254, 532
MflI RGATCY 1 cut(s) 558
MhlI GDGCHC 2 cut(s) 351, 604
MluCI AATT 3 cut(s) 195, 306, 531
MlyI GAGTC 2 cut(s) 228, 636
MroI TCCGGA 1 cut(s) 561
MspI CCGG 1 cut(s) 562
MspR9I CCNGG 3 cut(s) 141, 391, 632
MvaI CCWGG 3 cut(s) 141, 391, 632
MwoI GCNNNNNNNGC 3 cut(s) 56, 107, 457
NdeII GATC 4 cut(s) 17, 151, 360, 558
NlaIII CATG 2 cut(s) 226, 289
NmeAIII GCCGAG 1 cut(s) 475
PfeI GAWTC 1 cut(s) 184
PkrI GCNGC 3 cut(s) 52, 283, 462
PleI GAGTC 2 cut(s) 227, 635
PpsI GAGTC 2 cut(s) 227, 635
PpuMI RGGWCCY 1 cut(s) 445
Psp5II RGGWCCY 1 cut(s) 445
Psp6I CCWGG 3 cut(s) 139, 389, 630
PspGI CCWGG 3 cut(s) 139, 389, 630
PspPI GGNCC 2 cut(s) 445, 638
PspPPI RGGWCCY 1 cut(s) 445
PsuI RGATCY 1 cut(s) 558
RsaI GTAC 3 cut(s) 145, 168, 554
RsaNI GTAC 3 cut(s) 144, 167, 553
SatI GCNGC 3 cut(s) 51, 282, 461
Sau3AI GATC 4 cut(s) 17, 151, 360, 558
Sau96I GGNCC 2 cut(s) 445, 638
SchI GAGTC 2 cut(s) 228, 636
ScrFI CCNGG 3 cut(s) 141, 391, 632
SduI GDGCHC 2 cut(s) 351, 604
SinI GGWCC 1 cut(s) 445
SmlI CTYRAG 1 cut(s) 621
SmoI CTYRAG 1 cut(s) 621
Sse9I AATT 3 cut(s) 195, 306, 531
SsiI CCGC 2 cut(s) 201, 394
SspMI CTAG 2 cut(s) 228, 329
StyD4I CCNGG 3 cut(s) 139, 389, 630
TaaI ACNGT 2 cut(s) 171, 581
TaiI ACGT 1 cut(s) 342
TaqI TCGA 2 cut(s) 182, 381
TasI AATT 3 cut(s) 195, 306, 531
TatI WGTACW 1 cut(s) 552
TfiI GAWTC 1 cut(s) 184
TscAI CASTG 3 cut(s) 194, 328, 586
TseI GCWGC 3 cut(s) 50, 281, 460
TspGWI ACGGA 2 cut(s) 30, 228
TspRI CASTG 3 cut(s) 194, 328, 586
VpaK11BI GGWCC 1 cut(s) 445
XapI RAATTY 2 cut(s) 306, 531
XspI CTAG 2 cut(s) 228, 329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.