Rmu_sc0001644.1_g000007

Necessary for protein translocation in the endoplasmic reticulum

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001644.1
OG0005902
Physical Location & Seq
Forward (+)
41030 .. 42067
1038 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 615 bp
atggctcaaggctcatctcagtcccaagcgacgtcgtcttccacgacctcaagcctcggcgtcatggctcctcgtggttcagctgctgccacagctggaatgcacagccgtcgtatcggtggaggcgacaactccagctccagaggcatgggctgcagcggcagtggctctggtgccggaaacaacatactcaggttctacaccgacgacactcttggcttgaagatctcccccaccgtcatgctcgtcatgagcctatgcttcatcggcttcgtcactgccctccacgttttttgcaagctctacctctgggggtccagtgatgagctattgatgccaaggaggaaagttgggtcggagactattcgggtttgtaatggtttggacaagggcattgtggaagggggcggaatggttccgaagagggagagacaggagcatggtaggcggaatgaggatggtatgattggtacaaattatttggatgatggaggagaaggagaactaatttggaggcgtttgagttcaatgagtatgatgggattaagggacaaacgatcagaaagagtcggtttggtgatggagttatcgaagttgagtttggggagagaatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03060 ko04141 ko04145 ko05110 map03060 map04141 map04145 map05110
Pfam Domains
Protein Families
Sec61_beta

Protein Analysis

204

Amino Acids

21.78

Weight (kDa)

9.23

Isoelectric Point (pI)

57.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec61_beta PF03911 60 - 98 8.2e-14 Sec61beta family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 35
AccB1I GGYRCC 1 cut(s) 173
AciI CCGC 3 cut(s) 159, 408, 448
AcyI GRCGYC 2 cut(s) 32, 60
AfaI GTAC 1 cut(s) 472
AgsI TTSAA 2 cut(s) 223, 528
AjuI GAANNNNNNNTTGG 1 cut(s) 584
AluBI AGCT 5 cut(s) 83, 95, 138, 301, 328
AluI AGCT 5 cut(s) 83, 95, 138, 301, 328
Alw26I GTCTC 2 cut(s) 353, 424
AlwNI CAGNNNCTG 1 cut(s) 86
ApeKI GCWGC 4 cut(s) 83, 86, 153, 156
AspS9I GGNCC 1 cut(s) 315
AsuHPI GGTGA 1 cut(s) 589
AvaII GGWCC 1 cut(s) 315
BanI GGYRCC 1 cut(s) 173
BauI CACGAG 1 cut(s) 72
BbsI GAAGAC 1 cut(s) 30
BbvI GCAGC 4 cut(s) 70, 73, 140, 168
BccI CCATC 4 cut(s) 452, 482, 532, 574
BceAI ACGGC 1 cut(s) 93
BcgI CGANNNNNNTGC 2 cut(s) 92, 126
BcoDI GTCTC 2 cut(s) 353, 424
BfmI CTRYAG 1 cut(s) 154
BglII AGATCT 1 cut(s) 225
BisI GCNGC 5 cut(s) 84, 87, 154, 157, 160
BlsI GCNGC 5 cut(s) 85, 88, 155, 158, 161
Bme18I GGWCC 1 cut(s) 315
BmgT120I GGNCC 1 cut(s) 315
BmiI GGNNCC 4 cut(s) 69, 175, 316, 417
BmsI GCATC 1 cut(s) 324
BpiI GAAGAC 1 cut(s) 30
BpmI CTGGAG 2 cut(s) 118, 124
BpuEI CTTGAG 1 cut(s) 34
BsaHI GRCGYC 2 cut(s) 32, 60
BsaJI CCNNGG 2 cut(s) 55, 338
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bse1I ACTGG 1 cut(s) 318
BseDI CCNNGG 2 cut(s) 55, 338
BseGI GGATG 2 cut(s) 463, 490
BseMII CTCAG 2 cut(s) 32, 205
BseNI ACTGG 1 cut(s) 318
BseRI GAGGAG 2 cut(s) 60, 507
BseXI GCAGC 4 cut(s) 70, 73, 140, 168
BshNI GGYRCC 1 cut(s) 173
BsiSI CCGG 1 cut(s) 177
BslFI GGGAC 2 cut(s) 7, 563
BsmAI GTCTC 2 cut(s) 353, 424
BsmFI GGGAC 2 cut(s) 7, 563
BsmI GAATGC 1 cut(s) 105
Bsp143I GATC 2 cut(s) 225, 557
BspACI CCGC 3 cut(s) 159, 408, 448
BspCNI CTCAG 2 cut(s) 31, 204
BspHI TCATGA 1 cut(s) 249
BspLI GGNNCC 4 cut(s) 69, 175, 316, 417
BspMAI CTGCAG 1 cut(s) 158
BspT107I GGYRCC 1 cut(s) 173
BsrI ACTGG 1 cut(s) 318
BssECI CCNNGG 2 cut(s) 55, 338
BssMI GATC 2 cut(s) 225, 557
BssNI GRCGYC 2 cut(s) 32, 60
BssSI CACGAG 1 cut(s) 72
BssT1I CCWWGG 1 cut(s) 338
Bst2BI CACGAG 1 cut(s) 72
Bst4CI ACNGT 1 cut(s) 238
Bst6I CTCTTC 1 cut(s) 416
BstACI GRCGYC 2 cut(s) 32, 60
BstAPI GCANNNNNTGC 1 cut(s) 153
BstC8I GCNNGC 1 cut(s) 299
BstDEI CTNAG 2 cut(s) 18, 191
BstF5I GGATG 2 cut(s) 463, 490
BstKTI GATC 2 cut(s) 228, 560
BstMAI GTCTC 2 cut(s) 353, 424
BstMBI GATC 2 cut(s) 225, 557
BstMWI GCNNNNNNNGC 8 cut(s) 92, 144, 153, 159, 165, 267, 334, 445
BstSFI CTRYAG 1 cut(s) 154
BstV1I GCAGC 4 cut(s) 70, 73, 140, 168
BstV2I GAAGAC 1 cut(s) 30
BstX2I RGATCY 1 cut(s) 225
BstXI CCANNNNNNTGG 1 cut(s) 148
BstYI RGATCY 1 cut(s) 225
BtsCI GGATG 2 cut(s) 463, 490
BtsI GCAGTG 2 cut(s) 169, 276
BtsIMutI CAGTG 3 cut(s) 169, 276, 325
Cac8I GCNNGC 1 cut(s) 299
CaiI CAGNNNCTG 1 cut(s) 86
CciI TCATGA 1 cut(s) 249
Cfr13I GGNCC 1 cut(s) 315
CseI GACGC 1 cut(s) 49
Csp6I GTAC 1 cut(s) 471
CspCI CAANNNNNGTGG 2 cut(s) 275, 310
CviAII CATG 5 cut(s) 64, 148, 241, 250, 440
CviQI GTAC 1 cut(s) 471
DdeI CTNAG 2 cut(s) 18, 191
DpnI GATC 2 cut(s) 227, 559
DpnII GATC 2 cut(s) 225, 557
Eam1104I CTCTTC 1 cut(s) 416
EarI CTCTTC 1 cut(s) 416
EciI GGCGGA 2 cut(s) 423, 463
Eco130I CCWWGG 1 cut(s) 338
Eco47I GGWCC 1 cut(s) 315
EcoT14I CCWWGG 1 cut(s) 338
ErhI CCWWGG 1 cut(s) 338
FaeI CATG 5 cut(s) 67, 151, 244, 253, 443
FaiI YATR 9 cut(s) 65, 149, 188, 242, 251, 259, 441, 464, 536
FaqI GGGAC 2 cut(s) 7, 563
FatI CATG 5 cut(s) 63, 147, 240, 249, 439
Fnu4HI GCNGC 5 cut(s) 84, 87, 154, 157, 160
FokI GGATG 2 cut(s) 470, 497
Fsp4HI GCNGC 5 cut(s) 84, 87, 154, 157, 160
GluI GCNGC 5 cut(s) 84, 87, 154, 157, 160
GsuI CTGGAG 2 cut(s) 118, 124
HapII CCGG 1 cut(s) 177
HgaI GACGC 1 cut(s) 49
Hin1I GRCGYC 2 cut(s) 32, 60
Hin1II CATG 5 cut(s) 67, 151, 244, 253, 443
HinfI GANTC 1 cut(s) 567
HpaII CCGG 1 cut(s) 177
HphI GGTGA 1 cut(s) 589
Hpy188I TCNGA 3 cut(s) 358, 420, 562
Hpy188III TCNNGA 2 cut(s) 141, 250
Hpy99I CGWCG 4 cut(s) 34, 37, 114, 209
HpyAV CCTTC 2 cut(s) 395, 491
HpyCH4III ACNGT 1 cut(s) 238
HpyCH4IV ACGT 2 cut(s) 32, 288
HpyCH4V TGCA 3 cut(s) 103, 156, 297
HpyF10VI GCNNNNNNNGC 8 cut(s) 92, 144, 153, 159, 165, 267, 334, 445
HpyF3I CTNAG 2 cut(s) 18, 191
HpySE526I ACGT 2 cut(s) 32, 288
Hsp92I GRCGYC 2 cut(s) 32, 60
Hsp92II CATG 5 cut(s) 67, 151, 244, 253, 443
Kzo9I GATC 2 cut(s) 225, 557
LmnI GCTCC 3 cut(s) 73, 143, 436
LpnPI CCDG 9 cut(s) 81, 148, 154, 156, 178, 190, 295, 331, 419
Lsp1109I GCAGC 4 cut(s) 70, 73, 140, 168
LweI GCATC 1 cut(s) 324
MaeII ACGT 2 cut(s) 32, 288
MaeIII GTNAC 1 cut(s) 274
MalI GATC 2 cut(s) 227, 559
MboI GATC 2 cut(s) 225, 557
MboII GAAGA 3 cut(s) 30, 235, 433
MflI RGATCY 1 cut(s) 225
MluCI AATT 2 cut(s) 475, 507
MlyI GAGTC 1 cut(s) 576
MmeI TCCRAC 1 cut(s) 336
MseI TTAA 1 cut(s) 545
MslI CAYNNNNRTG 1 cut(s) 239
MspA1I CMGCKG 3 cut(s) 83, 95, 159
MspI CCGG 1 cut(s) 177
Mva1269I GAATGC 1 cut(s) 105
MwoI GCNNNNNNNGC 8 cut(s) 92, 144, 153, 159, 165, 267, 334, 445
NdeII GATC 2 cut(s) 225, 557
NlaIII CATG 5 cut(s) 67, 151, 244, 253, 443
NlaIV GGNNCC 4 cut(s) 69, 175, 316, 417
NmeAIII GCCGAG 1 cut(s) 36
NmuCI GTSAC 1 cut(s) 274
PagI TCATGA 1 cut(s) 249
PcsI WCGNNNNNNNCGW 2 cut(s) 41, 123
PctI GAATGC 1 cut(s) 105
PflFI GACNNNGTC 1 cut(s) 34
PkrI GCNGC 5 cut(s) 85, 88, 155, 158, 161
PleI GAGTC 1 cut(s) 575
PpsI GAGTC 1 cut(s) 575
PspN4I GGNNCC 4 cut(s) 69, 175, 316, 417
PspPI GGNCC 1 cut(s) 315
PstI CTGCAG 1 cut(s) 158
PstNI CAGNNNCTG 1 cut(s) 86
PsuI RGATCY 1 cut(s) 225
PsyI GACNNNGTC 1 cut(s) 34
PvuII CAGCTG 2 cut(s) 83, 95
RsaI GTAC 1 cut(s) 472
RsaNI GTAC 1 cut(s) 471
RseI CAYNNNNRTG 1 cut(s) 239
SaqAI TTAA 1 cut(s) 545
SatI GCNGC 5 cut(s) 84, 87, 154, 157, 160
Sau3AI GATC 2 cut(s) 225, 557
Sau96I GGNCC 1 cut(s) 315
SchI GAGTC 1 cut(s) 576
SfaNI GCATC 1 cut(s) 324
SfcI CTRYAG 1 cut(s) 154
SinI GGWCC 1 cut(s) 315
SmiMI CAYNNNNRTG 1 cut(s) 239
SmlI CTYRAG 2 cut(s) 6, 49
SmoI CTYRAG 2 cut(s) 6, 49
Sse9I AATT 2 cut(s) 475, 507
SsiI CCGC 3 cut(s) 159, 408, 448
StyI CCWWGG 1 cut(s) 338
TaaI ACNGT 1 cut(s) 238
TaiI ACGT 2 cut(s) 35, 291
TaqI TCGA 1 cut(s) 590
TasI AATT 2 cut(s) 475, 507
TauI GCSGC 1 cut(s) 162
Tru1I TTAA 1 cut(s) 545
Tru9I TTAA 1 cut(s) 545
TscAI CASTG 3 cut(s) 169, 283, 325
TseFI GTSAC 1 cut(s) 274
TseI GCWGC 4 cut(s) 83, 86, 153, 156
Tsp45I GTSAC 1 cut(s) 274
TspDTI ATGAA 1 cut(s) 253
TspRI CASTG 3 cut(s) 169, 283, 325
Tth111I GACNNNGTC 1 cut(s) 34
VpaK11BI GGWCC 1 cut(s) 315
ZraI GACGTC 1 cut(s) 33
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.