Rmu_sc0002321.1_g000020

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002321.1
OG0002478
Physical Location & Seq
Reverse (-)
151515 .. 158320
6806 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1512 bp
atgttgcttgtccagagtccaagtcatttgttttacttgataaattactcgacccaagaaggaaacagtgcagttgggattgggacccagcagcaacagcagctcccacatttaaaaaagttgaggcttgttggaatgtacaagctgatgcatctagggcaggatgatgaggaggataactcacaatcagccccccgcatcccaaattttccgaacctgcaaattctattcgtgaatgcttgtgacagcttaaggaatctgagatcatccgcaatatccttaaacaatctaacaagcttggaagtaagtttttgcgatggattgaaatacttgataacttactccatggccaaaagtttaatgcaactcacaacactggaagtcaagaagtgcccaagactggtggagatagtgggaagcgatggagacgacgattcaagacatgagattacattcaggcggttgaaacatttgaaactttctattctgccaagactgcaacgcttttgctccggaaattgcattgctaaattccctatctttcatgctgatattttgtccgatctcccatataacaggtataacataccagagttggctaaaaaatacaaggtttatgctatagacttgctgggcttcggttggagtgaaaaagcactggttgaatatgatgccatgatttggaaagatcaagtagtggacttcttgaaggagatagtgaaagaaccaacagttttggttggaaatagcctaggaggttttactgctttggttgcagcagttggattgcctgagcaagtagttggtgttgcactattgaattcggcaggacagtttggaaatgcaaatagtgaacctgagaagtctgacgaggaaacagtcctacagaagttcaacataaagccactgaaggaagttttccagcgagtagtacttggatttttattctggcaagcaaagcagcctgctcgcattgaatccgttttaaagagtgtgtacaaaaatacctccaatgtggatgactatctggtggattccataacaatgccagcagctgaccccaatgccggagaagtctactacagtatctggtatgcagacagctcaagtggaattgatgcttctgaagtttcgtcggagctaaaatcaaatggacaagttgtggatgaaagtctattcaaagatagcaagatggatgtgactgaggtacaaatagatggttcaatgcaaaaatctcagctgaggataaaggaacctcattgtgaaggagcacaagatttggtagttgccaacgattctaattatgttactattgttcaatctgcgttgccacataaaggaagcgaatccccatgtgctgtgaaaactgttgagcccgtttcattgactctgagttcaactctcactaagagacaggcaagcaagagttcaaatgtgaatgtggctgccactgtaatagagcctaaagaagcttcaaggctgaggcattgggtgattaagatgggaaattag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

503

Amino Acids

55.86

Weight (kDa)

5.93

Isoelectric Point (pI)

48.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_RPS2 PF23247 93 - 168 7e-09 Plant disease resistance protein RPS2-like, leucine-rich repeats
Abhydrolase_1 PF00561 188 - 298 4.1e-12 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 197 - 279 1.1e-09 Alpha/beta hydrolase family
Hydrolase_4 PF12146 201 - 283 2.3e-06 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 225
AccB7I CCANNNNNTGG 1 cut(s) 681
AccI GTMKAC 1 cut(s) 1077
AccIII TCCGGA 1 cut(s) 512
AciI CCGC 3 cut(s) 196, 270, 460
AcoI YGGCCR 1 cut(s) 348
AcsI RAATTY 4 cut(s) 205, 222, 530, 820
AcuI CTGAAG 2 cut(s) 929, 1146
AfaI GTAC 4 cut(s) 140, 933, 998, 1209
AfiI CCNNNNNNNGG 4 cut(s) 400, 681, 1067, 1337
AflII CTTAAG 1 cut(s) 250
AluBI AGCT 9 cut(s) 103, 145, 249, 297, 1055, 1104, 1141, 1240, 1472
AluI AGCT 9 cut(s) 103, 145, 249, 297, 1055, 1104, 1141, 1240, 1472
Alw21I GWGCWC 1 cut(s) 1273
Alw26I GTCTC 2 cut(s) 420, 1405
AlwNI CAGNNNCTG 2 cut(s) 1055, 1089
Aor13HI TCCGGA 1 cut(s) 512
AoxI GGCC 1 cut(s) 348
ApeKI GCWGC 6 cut(s) 91, 100, 776, 961, 1052, 1445
ApoI RAATTY 4 cut(s) 205, 222, 530, 820
AspA2I CCTAGG 1 cut(s) 751
AspS9I GGNCC 1 cut(s) 84
AsuHPI GGTGA 1 cut(s) 1504
AvaII GGWCC 1 cut(s) 84
AvrII CCTAGG 1 cut(s) 751
BaeGI GKGCMC 1 cut(s) 395
BaeI ACNNNNGTAYC 2 cut(s) 1069, 1102
BalI TGGCCA 1 cut(s) 350
BanII GRGCYC 1 cut(s) 1377
Bbv12I GWGCWC 1 cut(s) 1273
BbvCI CCTCAGC 2 cut(s) 1241, 1481
BbvI GCAGC 6 cut(s) 103, 112, 788, 973, 1064, 1432
BccI CCATC 5 cut(s) 311, 416, 1186, 1211, 1495
BcgI CGANNNNNNTGC 2 cut(s) 950, 984
BcoDI GTCTC 2 cut(s) 420, 1405
BfaI CTAG 2 cut(s) 155, 752
BfmI CTRYAG 3 cut(s) 621, 884, 1081
BfrI CTTAAG 1 cut(s) 250
BfuAI ACCTGC 1 cut(s) 225
BisI GCNGC 6 cut(s) 92, 101, 777, 962, 1053, 1446
BlnI CCTAGG 1 cut(s) 751
BlsI GCNGC 6 cut(s) 93, 102, 778, 963, 1054, 1447
BmcAI AGTACT 1 cut(s) 933
Bme18I GGWCC 1 cut(s) 84
BmgT120I GGNCC 1 cut(s) 84
BmiI GGNNCC 3 cut(s) 85, 86, 1254
BmsI GCATC 5 cut(s) 138, 160, 207, 661, 1108
BplI GAGNNNNNCTC 4 cut(s) 164, 196, 1384, 1416
Bpu10I CCTNAGC 3 cut(s) 792, 1241, 1481
BpuEI CTTGAG 1 cut(s) 1090
BsaBI GATNNNNATC 1 cut(s) 1023
BsaJI CCNNGG 2 cut(s) 345, 751
BsaWI WCCGGW 1 cut(s) 512
BsaXI ACNNNNNCTCC 2 cut(s) 87, 117
Bsc4I CCNNNNNNNGG 4 cut(s) 400, 681, 1067, 1337
Bse1I ACTGG 3 cut(s) 381, 405, 663
Bse3DI GCAATG 1 cut(s) 522
Bse8I GATNNNNATC 1 cut(s) 1023
BseAI TCCGGA 1 cut(s) 512
BseDI CCNNGG 2 cut(s) 345, 751
BseGI GGATG 6 cut(s) 169, 198, 266, 1024, 1171, 1201
BseJI GATNNNNATC 1 cut(s) 1023
BseLI CCNNNNNNNGG 4 cut(s) 400, 681, 1067, 1337
BseMI GCAATG 1 cut(s) 522
BseMII CTCAG 8 cut(s) 251, 783, 849, 1194, 1232, 1250, 1382, 1472
BseNI ACTGG 3 cut(s) 381, 405, 663
BseRI GAGGAG 1 cut(s) 185
BseSI GKGCMC 1 cut(s) 395
BseXI GCAGC 6 cut(s) 103, 112, 788, 973, 1064, 1432
BseYI CCCAGC 2 cut(s) 87, 631
BsgI GTGCAG 1 cut(s) 90
BshFI GGCC 1 cut(s) 350
BsiHKAI GWGCWC 1 cut(s) 1273
BsiSI CCGG 2 cut(s) 513, 1068
BslFI GGGAC 1 cut(s) 97
BslI CCNNNNNNNGG 4 cut(s) 400, 681, 1067, 1337
BsmAI GTCTC 2 cut(s) 420, 1405
BsmBI CGTCTC 1 cut(s) 420
BsmFI GGGAC 1 cut(s) 97
BsmI GAATGC 1 cut(s) 241
BsnI GGCC 1 cut(s) 350
Bsp1286I GDGCHC 3 cut(s) 395, 1273, 1377
Bsp13I TCCGGA 1 cut(s) 512
Bsp1407I TGTACA 2 cut(s) 138, 996
Bsp143I GATC 3 cut(s) 263, 562, 688
Bsp19I CCATGG 1 cut(s) 345
BspACI CCGC 3 cut(s) 196, 270, 460
BspANI GGCC 1 cut(s) 350
BspCNI CTCAG 8 cut(s) 252, 784, 850, 1195, 1233, 1249, 1383, 1473
BspEI TCCGGA 1 cut(s) 512
BspLI GGNNCC 3 cut(s) 85, 86, 1254
BspMI ACCTGC 1 cut(s) 225
BspTI CTTAAG 1 cut(s) 250
BsrDI GCAATG 1 cut(s) 522
BsrGI TGTACA 2 cut(s) 138, 996
BsrI ACTGG 3 cut(s) 381, 405, 663
BssECI CCNNGG 2 cut(s) 345, 751
BssMI GATC 3 cut(s) 263, 562, 688
BssT1I CCWWGG 2 cut(s) 345, 751
Bst4CI ACNGT 7 cut(s) 68, 733, 834, 880, 1085, 1369, 1453
BstAFI CTTAAG 1 cut(s) 250
BstAUI TGTACA 2 cut(s) 138, 996
BstC8I GCNNGC 5 cut(s) 954, 966, 970, 1050, 1420
BstDEI CTNAG 9 cut(s) 260, 792, 858, 1203, 1236, 1241, 1391, 1407, 1481
BstDSI CCRYGG 1 cut(s) 345
BstF5I GGATG 6 cut(s) 169, 198, 266, 1024, 1171, 1201
BstKTI GATC 3 cut(s) 266, 565, 691
BstMAI GTCTC 2 cut(s) 420, 1405
BstMBI GATC 3 cut(s) 263, 562, 688
BstMWI GCNNNNNNNGC 6 cut(s) 97, 100, 157, 496, 773, 958
BstSFI CTRYAG 3 cut(s) 621, 884, 1081
BstSLI GKGCMC 1 cut(s) 395
BstV1I GCAGC 6 cut(s) 103, 112, 788, 973, 1064, 1432
BsuRI GGCC 1 cut(s) 350
BtgI CCRYGG 1 cut(s) 345
BtgZI GCGATG 2 cut(s) 330, 435
BtsCI GGATG 6 cut(s) 169, 198, 266, 1024, 1171, 1201
BtsIMutI CAGTG 5 cut(s) 73, 374, 656, 905, 1449
BveI ACCTGC 1 cut(s) 225
Cac8I GCNNGC 5 cut(s) 954, 966, 970, 1050, 1420
CaiI CAGNNNCTG 2 cut(s) 1055, 1089
Cfr13I GGNCC 1 cut(s) 84
Csp6I GTAC 4 cut(s) 139, 932, 997, 1208
CspCI CAANNNNNGTGG 2 cut(s) 384, 419
CviAII CATG 5 cut(s) 346, 443, 545, 676, 1353
CviQI GTAC 4 cut(s) 139, 932, 997, 1208
DdeI CTNAG 9 cut(s) 260, 792, 858, 1203, 1236, 1241, 1391, 1407, 1481
DpnI GATC 3 cut(s) 265, 564, 690
DpnII GATC 3 cut(s) 263, 562, 688
DraI TTTAAA 2 cut(s) 114, 987
EaeI YGGCCR 1 cut(s) 348
Eco130I CCWWGG 2 cut(s) 345, 751
Eco24I GRGCYC 1 cut(s) 1377
Eco47I GGWCC 1 cut(s) 84
Eco57I CTGAAG 2 cut(s) 929, 1146
EcoO109I RGGNCCY 1 cut(s) 84
EcoRI GAATTC 1 cut(s) 820
EcoT14I CCWWGG 2 cut(s) 345, 751
EcoT22I ATGCAT 1 cut(s) 153
EcoT38I GRGCYC 1 cut(s) 1377
ErhI CCWWGG 2 cut(s) 345, 751
Esp3I CGTCTC 1 cut(s) 420
FaeI CATG 5 cut(s) 349, 446, 548, 679, 1356
FaqI GGGAC 1 cut(s) 97
FatI CATG 5 cut(s) 345, 442, 544, 675, 1352
FauI CCCGC 1 cut(s) 203
FblI GTMKAC 1 cut(s) 1077
Fnu4HI GCNGC 6 cut(s) 92, 101, 777, 962, 1053, 1446
FokI GGATG 6 cut(s) 176, 185, 253, 1031, 1178, 1208
FriOI GRGCYC 1 cut(s) 1377
Fsp4HI GCNGC 6 cut(s) 92, 101, 777, 962, 1053, 1446
FspBI CTAG 2 cut(s) 155, 752
GluI GCNGC 6 cut(s) 92, 101, 777, 962, 1053, 1446
GsaI CCCAGC 2 cut(s) 91, 635
HaeIII GGCC 1 cut(s) 350
HapII CCGG 2 cut(s) 513, 1068
Hin1II CATG 5 cut(s) 349, 446, 548, 679, 1356
HindIII AAGCTT 2 cut(s) 295, 1470
HinfI GANTC 8 cut(s) 16, 256, 434, 977, 1034, 1295, 1346, 1387
HpaII CCGG 2 cut(s) 513, 1068
HphI GGTGA 1 cut(s) 1504
Hpy166II GTNNAC 4 cut(s) 700, 854, 997, 1078
Hpy188I TCNGA 7 cut(s) 213, 261, 562, 868, 1126, 1138, 1392
Hpy188III TCNNGA 6 cut(s) 13, 232, 385, 438, 513, 706
Hpy8I GTNNAC 4 cut(s) 700, 854, 997, 1078
Hpy99I CGWCG 2 cut(s) 434, 1138
HpyAV CCTTC 4 cut(s) 53, 703, 904, 1259
HpyCH4III ACNGT 7 cut(s) 68, 733, 834, 880, 1085, 1369, 1453
HpyF10VI GCNNNNNNNGC 6 cut(s) 97, 100, 157, 496, 773, 958
HpyF3I CTNAG 9 cut(s) 260, 792, 858, 1203, 1236, 1241, 1391, 1407, 1481
Hsp92II CATG 5 cut(s) 349, 446, 548, 679, 1356
KflI GGGWCCC 1 cut(s) 84
Kpn2I TCCGGA 1 cut(s) 512
Kzo9I GATC 3 cut(s) 263, 562, 688
LmnI GCTCC 4 cut(s) 108, 515, 1138, 1268
Lsp1109I GCAGC 6 cut(s) 103, 112, 788, 973, 1064, 1432
LweI GCATC 5 cut(s) 138, 160, 207, 661, 1108
MaeI CTAG 2 cut(s) 155, 752
MaeIII GTNAC 3 cut(s) 242, 1198, 1306
MalI GATC 3 cut(s) 265, 564, 690
MboI GATC 3 cut(s) 263, 562, 688
MhlI GDGCHC 3 cut(s) 395, 1273, 1377
MlsI TGGCCA 1 cut(s) 350
MluCI AATT 9 cut(s) 43, 205, 222, 517, 530, 820, 1113, 1300, 1507
MluNI TGGCCA 1 cut(s) 350
MlyI GAGTC 2 cut(s) 25, 1381
MmeI TCCRAC 5 cut(s) 112, 623, 721, 763, 1116
Mox20I TGGCCA 1 cut(s) 350
Mph1103I ATGCAT 1 cut(s) 153
MroI TCCGGA 1 cut(s) 512
MscI TGGCCA 1 cut(s) 350
MseI TTAA 6 cut(s) 113, 251, 281, 359, 986, 1497
MslI CAYNNNNRTG 1 cut(s) 1043
Msp20I TGGCCA 1 cut(s) 350
MspA1I CMGCKG 2 cut(s) 1055, 1240
MspCI CTTAAG 1 cut(s) 250
MspI CCGG 2 cut(s) 513, 1068
Mva1269I GAATGC 1 cut(s) 241
MwoI GCNNNNNNNGC 6 cut(s) 97, 100, 157, 496, 773, 958
NcoI CCATGG 1 cut(s) 345
NdeII GATC 3 cut(s) 263, 562, 688
NlaIII CATG 5 cut(s) 349, 446, 548, 679, 1356
NlaIV GGNNCC 3 cut(s) 85, 86, 1254
NmuCI GTSAC 2 cut(s) 242, 1198
NsiI ATGCAT 1 cut(s) 153
PctI GAATGC 1 cut(s) 241
PfeI GAWTC 6 cut(s) 256, 434, 977, 1034, 1295, 1346
PflMI CCANNNNNTGG 1 cut(s) 681
PkrI GCNGC 6 cut(s) 93, 102, 778, 963, 1054, 1447
PleI GAGTC 2 cut(s) 24, 1381
PpsI GAGTC 2 cut(s) 24, 1381
PpuMI RGGWCCY 1 cut(s) 84
Psp5II RGGWCCY 1 cut(s) 84
PspFI CCCAGC 2 cut(s) 87, 631
PspN4I GGNNCC 3 cut(s) 85, 86, 1254
PspPI GGNCC 1 cut(s) 84
PspPPI RGGWCCY 1 cut(s) 84
PstNI CAGNNNCTG 2 cut(s) 1055, 1089
PvuII CAGCTG 2 cut(s) 1055, 1240
RsaI GTAC 4 cut(s) 140, 933, 998, 1209
RsaNI GTAC 4 cut(s) 139, 932, 997, 1208
RseI CAYNNNNRTG 1 cut(s) 1043
SaqAI TTAA 6 cut(s) 113, 251, 281, 359, 986, 1497
SatI GCNGC 6 cut(s) 92, 101, 777, 962, 1053, 1446
Sau3AI GATC 3 cut(s) 263, 562, 688
Sau96I GGNCC 1 cut(s) 84
ScaI AGTACT 1 cut(s) 933
SchI GAGTC 2 cut(s) 25, 1381
SduI GDGCHC 3 cut(s) 395, 1273, 1377
SfaNI GCATC 5 cut(s) 138, 160, 207, 661, 1108
SfcI CTRYAG 3 cut(s) 621, 884, 1081
SinI GGWCC 1 cut(s) 84
SmiMI CAYNNNNRTG 1 cut(s) 1043
SmlI CTYRAG 2 cut(s) 250, 1105
SmoI CTYRAG 2 cut(s) 250, 1105
Sse9I AATT 9 cut(s) 43, 205, 222, 517, 530, 820, 1113, 1300, 1507
SsiI CCGC 3 cut(s) 196, 270, 460
SspMI CTAG 2 cut(s) 155, 752
StyI CCWWGG 2 cut(s) 345, 751
TaaI ACNGT 7 cut(s) 68, 733, 834, 880, 1085, 1369, 1453
TaqI TCGA 1 cut(s) 50
TasI AATT 9 cut(s) 43, 205, 222, 517, 530, 820, 1113, 1300, 1507
TatI WGTACW 3 cut(s) 138, 931, 996
TfiI GAWTC 6 cut(s) 256, 434, 977, 1034, 1295, 1346
Tru1I TTAA 6 cut(s) 113, 251, 281, 359, 986, 1497
Tru9I TTAA 6 cut(s) 113, 251, 281, 359, 986, 1497
TscAI CASTG 5 cut(s) 73, 381, 663, 912, 1456
TseFI GTSAC 2 cut(s) 242, 1198
TseI GCWGC 6 cut(s) 91, 100, 776, 961, 1052, 1445
Tsp45I GTSAC 2 cut(s) 242, 1198
TspDTI ATGAA 3 cut(s) 533, 1182, 1371
TspGWI ACGGA 1 cut(s) 970
TspRI CASTG 5 cut(s) 73, 381, 663, 912, 1456
Van91I CCANNNNNTGG 1 cut(s) 681
Vha464I CTTAAG 1 cut(s) 250
VpaK11BI GGWCC 1 cut(s) 84
XapI RAATTY 4 cut(s) 205, 222, 530, 820
XmaJI CCTAGG 1 cut(s) 751
XmiI GTMKAC 1 cut(s) 1077
XspI CTAG 2 cut(s) 155, 752
ZrmI AGTACT 1 cut(s) 933
Zsp2I ATGCAT 1 cut(s) 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.