Rmu_sc0002539.1_g000114

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002539.1
OG0010602
Physical Location & Seq
Reverse (-)
469781 .. 473307
3527 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 729 bp
atggcttctcttgctacccttatgaagccatggtgtagagatggtggcctagataccggaaatgtgtgtgcctcctccttgtgcaagcttacatgtgaagaatgggggatgaagaagagaagaataggtgagaagggagaggagtcccaaaattcggccaaaaagcctagggtgaaggaatttggtcttaagcttgtgaaatgtgtgtgcaaaggtgcttacatacccaatcaagggccagctgtcaaactgactcaacttggaggcatcgcactcgtccacgtgtacggacacacgttcttctctcatggagtcattatccttcctatgtgggacccgtctataaccaccgcacgagctaaagttcgtacgctcaaggtgaccgagagatcaagccgaggtgaccataagatgatggctagtgagatcctaccgagtgagtatggaatctcaaatatacactacaacgtaacaagtggttaccagtaccccataccagacaagtcggctcctgtctacataactgttgaagatggagggaaccaagaaggtcttgctggaaggtctagagatccacaaccagaatattctgcattccgagaagcgagctatgggcattcgacactggagattcataaccggacacatgcactttaccactggaaccgcaatgatgacgggaagaaaatggctactgacgcatttgtattgcataaccagtactggtga

Protein Analysis

242

Amino Acids

27.0

Weight (kDa)

9.04

Isoelectric Point (pI)

38.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos_C PF14008 170 - 228 9.7e-19 Iron/zinc purple acid phosphatase-like protein C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 516
AciI CCGC 2 cut(s) 351, 667
AclWI GGATC 2 cut(s) 421, 566
AcoI YGGCCR 1 cut(s) 156
AcsI RAATTY 2 cut(s) 151, 179
AcvI CACGTG 1 cut(s) 283
AfaI GTAC 4 cut(s) 287, 370, 488, 722
AfiI CCNNNNNNNGG 3 cut(s) 154, 233, 234
AflII CTTAAG 1 cut(s) 188
AflIII ACRYGT 3 cut(s) 92, 282, 294
AgsI TTSAA 1 cut(s) 530
AluBI AGCT 5 cut(s) 88, 193, 242, 359, 609
AluI AGCT 5 cut(s) 88, 193, 242, 359, 609
AlwI GGATC 2 cut(s) 421, 566
AoxI GGCC 3 cut(s) 46, 156, 236
ApoI RAATTY 2 cut(s) 151, 179
AspA2I CCTAGG 1 cut(s) 167
AspS9I GGNCC 2 cut(s) 236, 334
AsuHPI GGTGA 4 cut(s) 140, 184, 391, 413
AvaII GGWCC 1 cut(s) 334
AvrII CCTAGG 1 cut(s) 167
BauI CACGAG 1 cut(s) 354
BbrPI CACGTG 1 cut(s) 283
BccI CCATC 3 cut(s) 35, 409, 527
BcgI CGANNNNNNTGC 2 cut(s) 256, 290
BfaI CTAG 4 cut(s) 50, 168, 420, 567
BfrI CTTAAG 1 cut(s) 188
BlnI CCTAGG 1 cut(s) 167
BmcAI AGTACT 1 cut(s) 722
Bme18I GGWCC 1 cut(s) 334
BmgT120I GGNCC 2 cut(s) 236, 334
BmiI GGNNCC 5 cut(s) 335, 336, 510, 542, 665
BmsI GCATC 1 cut(s) 276
BpmI CTGGAG 1 cut(s) 647
BpuEI CTTGAG 1 cut(s) 359
BsaAI YACGTR 1 cut(s) 283
BsaJI CCNNGG 3 cut(s) 29, 167, 397
BsaWI WCCGGW 2 cut(s) 56, 639
Bsc4I CCNNNNNNNGG 3 cut(s) 154, 233, 234
Bse1I ACTGG 5 cut(s) 484, 630, 665, 718, 728
Bse3DI GCAATG 1 cut(s) 676
BseDI CCNNGG 3 cut(s) 29, 167, 397
BseGI GGATG 1 cut(s) 114
BseLI CCNNNNNNNGG 3 cut(s) 154, 233, 234
BseMI GCAATG 1 cut(s) 676
BseNI ACTGG 5 cut(s) 484, 630, 665, 718, 728
BseRI GAGGAG 2 cut(s) 64, 155
BshFI GGCC 3 cut(s) 48, 158, 238
BsiSI CCGG 2 cut(s) 57, 640
BsiWI CGTACG 1 cut(s) 368
BslFI GGGAC 2 cut(s) 130, 347
BslI CCNNNNNNNGG 3 cut(s) 154, 233, 234
BsmFI GGGAC 2 cut(s) 130, 347
BsmI GAATGC 2 cut(s) 593, 616
BsnI GGCC 3 cut(s) 48, 158, 238
Bsp143I GATC 3 cut(s) 389, 426, 571
Bsp19I CCATGG 1 cut(s) 29
BspACI CCGC 2 cut(s) 351, 667
BspANI GGCC 3 cut(s) 48, 158, 238
BspLI GGNNCC 5 cut(s) 335, 336, 510, 542, 665
BspPI GGATC 2 cut(s) 421, 566
BspTI CTTAAG 1 cut(s) 188
BsrDI GCAATG 1 cut(s) 676
BsrI ACTGG 5 cut(s) 484, 630, 665, 718, 728
BssECI CCNNGG 3 cut(s) 29, 167, 397
BssMI GATC 3 cut(s) 389, 426, 571
BssSI CACGAG 1 cut(s) 354
BssT1I CCWWGG 2 cut(s) 29, 167
Bst2BI CACGAG 1 cut(s) 354
Bst4CI ACNGT 1 cut(s) 526
Bst6I CTCTTC 1 cut(s) 110
BstAFI CTTAAG 1 cut(s) 188
BstBAI YACGTR 1 cut(s) 283
BstC8I GCNNGC 3 cut(s) 86, 240, 607
BstDSI CCRYGG 1 cut(s) 29
BstEII GGTNACC 3 cut(s) 379, 401, 479
BstF5I GGATG 1 cut(s) 114
BstKTI GATC 3 cut(s) 392, 429, 574
BstMBI GATC 3 cut(s) 389, 426, 571
BstMWI GCNNNNNNNGC 2 cut(s) 11, 698
BstNSI RCATGY 2 cut(s) 96, 650
BstPI GGTNACC 3 cut(s) 379, 401, 479
BstX2I RGATCY 2 cut(s) 426, 571
BstYI RGATCY 2 cut(s) 426, 571
BsuRI GGCC 3 cut(s) 48, 158, 238
BtgI CCRYGG 1 cut(s) 29
BtgZI GCGATG 1 cut(s) 253
BtsCI GGATG 1 cut(s) 114
BtsIMutI CAGTG 2 cut(s) 623, 658
Cac8I GCNNGC 3 cut(s) 86, 240, 607
Cfr13I GGNCC 2 cut(s) 236, 334
CseI GACGC 1 cut(s) 707
Csp6I GTAC 4 cut(s) 286, 369, 487, 721
CviAII CATG 4 cut(s) 30, 93, 308, 647
CviQI GTAC 4 cut(s) 286, 369, 487, 721
DpnI GATC 3 cut(s) 391, 428, 573
DpnII GATC 3 cut(s) 389, 426, 571
EaeI YGGCCR 1 cut(s) 156
Eam1104I CTCTTC 1 cut(s) 110
EarI CTCTTC 1 cut(s) 110
Eco130I CCWWGG 2 cut(s) 29, 167
Eco47I GGWCC 1 cut(s) 334
Eco72I CACGTG 1 cut(s) 283
Eco91I GGTNACC 3 cut(s) 379, 401, 479
EcoO109I RGGNCCY 1 cut(s) 334
EcoO65I GGTNACC 3 cut(s) 379, 401, 479
EcoT14I CCWWGG 2 cut(s) 29, 167
ErhI CCWWGG 2 cut(s) 29, 167
FaeI CATG 4 cut(s) 33, 96, 311, 650
FalI AAGNNNNNCTT 2 cut(s) 537, 569
FaqI GGGAC 2 cut(s) 130, 347
FatI CATG 4 cut(s) 29, 92, 307, 646
FblI GTMKAC 1 cut(s) 516
FokI GGATG 1 cut(s) 121
FspBI CTAG 4 cut(s) 50, 168, 420, 567
GsuI CTGGAG 1 cut(s) 647
HaeIII GGCC 3 cut(s) 48, 158, 238
HapII CCGG 2 cut(s) 57, 640
HgaI GACGC 1 cut(s) 707
Hin1II CATG 4 cut(s) 33, 96, 311, 650
HindIII AAGCTT 2 cut(s) 86, 191
HinfI GANTC 5 cut(s) 143, 253, 312, 447, 631
HpaII CCGG 2 cut(s) 57, 640
HphI GGTGA 4 cut(s) 140, 184, 391, 413
Hpy166II GTNNAC 3 cut(s) 280, 286, 517
Hpy188I TCNGA 1 cut(s) 599
Hpy188III TCNNGA 1 cut(s) 567
Hpy8I GTNNAC 3 cut(s) 280, 286, 517
HpyAV CCTTC 5 cut(s) 127, 169, 332, 542, 555
HpyCH4III ACNGT 1 cut(s) 526
HpyCH4IV ACGT 3 cut(s) 282, 296, 468
HpyCH4V TGCA 5 cut(s) 84, 210, 593, 650, 712
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 698
HpySE526I ACGT 3 cut(s) 282, 296, 468
Hsp92II CATG 4 cut(s) 33, 96, 311, 650
KflI GGGWCCC 1 cut(s) 334
Kzo9I GATC 3 cut(s) 389, 426, 571
LmnI GCTCC 1 cut(s) 514
LweI GCATC 1 cut(s) 276
MaeI CTAG 4 cut(s) 50, 168, 420, 567
MaeII ACGT 3 cut(s) 282, 296, 468
MaeIII GTNAC 4 cut(s) 379, 401, 469, 479
MalI GATC 3 cut(s) 391, 428, 573
MboI GATC 3 cut(s) 389, 426, 571
MboII GAAGA 7 cut(s) 110, 124, 127, 132, 292, 542, 694
MflI RGATCY 2 cut(s) 426, 571
MluCI AATT 2 cut(s) 151, 179
MlyI GAGTC 3 cut(s) 152, 247, 321
MnlI CCTC 6 cut(s) 82, 85, 133, 257, 392, 530
MseI TTAA 1 cut(s) 189
MspA1I CMGCKG 1 cut(s) 242
MspCI CTTAAG 1 cut(s) 188
MspI CCGG 2 cut(s) 57, 640
Mva1269I GAATGC 2 cut(s) 593, 616
MwoI GCNNNNNNNGC 2 cut(s) 11, 698
NcoI CCATGG 1 cut(s) 29
NdeII GATC 3 cut(s) 389, 426, 571
NlaIII CATG 4 cut(s) 33, 96, 311, 650
NlaIV GGNNCC 5 cut(s) 335, 336, 510, 542, 665
NmeAIII GCCGAG 1 cut(s) 422
NmuCI GTSAC 2 cut(s) 379, 401
NspI RCATGY 2 cut(s) 96, 650
PciI ACATGT 1 cut(s) 92
PctI GAATGC 2 cut(s) 593, 616
PfeI GAWTC 2 cut(s) 447, 631
Pfl23II CGTACG 1 cut(s) 368
PleI GAGTC 3 cut(s) 151, 247, 320
PmaCI CACGTG 1 cut(s) 283
PmlI CACGTG 1 cut(s) 283
PpsI GAGTC 3 cut(s) 151, 247, 320
Ppu21I YACGTR 1 cut(s) 283
PpuMI RGGWCCY 1 cut(s) 334
PscI ACATGT 1 cut(s) 92
Psp5II RGGWCCY 1 cut(s) 334
PspCI CACGTG 1 cut(s) 283
PspEI GGTNACC 3 cut(s) 379, 401, 479
PspLI CGTACG 1 cut(s) 368
PspN4I GGNNCC 5 cut(s) 335, 336, 510, 542, 665
PspPI GGNCC 2 cut(s) 236, 334
PspPPI RGGWCCY 1 cut(s) 334
PsuI RGATCY 2 cut(s) 426, 571
PvuII CAGCTG 1 cut(s) 242
RsaI GTAC 4 cut(s) 287, 370, 488, 722
RsaNI GTAC 4 cut(s) 286, 369, 487, 721
SaqAI TTAA 1 cut(s) 189
Sau3AI GATC 3 cut(s) 389, 426, 571
Sau96I GGNCC 2 cut(s) 236, 334
ScaI AGTACT 1 cut(s) 722
SchI GAGTC 3 cut(s) 152, 247, 321
SfaNI GCATC 1 cut(s) 276
SinI GGWCC 1 cut(s) 334
SmlI CTYRAG 2 cut(s) 188, 374
SmoI CTYRAG 2 cut(s) 188, 374
Sse9I AATT 2 cut(s) 151, 179
SsiI CCGC 2 cut(s) 351, 667
SspI AATATT 1 cut(s) 587
SspMI CTAG 4 cut(s) 50, 168, 420, 567
StyI CCWWGG 2 cut(s) 29, 167
TaaI ACNGT 1 cut(s) 526
TaiI ACGT 3 cut(s) 285, 299, 471
TaqI TCGA 1 cut(s) 620
TaqII GACCGA 1 cut(s) 398
TasI AATT 2 cut(s) 151, 179
TatI WGTACW 1 cut(s) 720
TfiI GAWTC 2 cut(s) 447, 631
Tru1I TTAA 1 cut(s) 189
Tru9I TTAA 1 cut(s) 189
TscAI CASTG 2 cut(s) 630, 665
TseFI GTSAC 2 cut(s) 379, 401
Tsp45I GTSAC 2 cut(s) 379, 401
TspDTI ATGAA 3 cut(s) 38, 125, 623
TspGWI ACGGA 1 cut(s) 303
TspRI CASTG 2 cut(s) 630, 665
Vha464I CTTAAG 1 cut(s) 188
VpaK11BI GGWCC 1 cut(s) 334
XapI RAATTY 2 cut(s) 151, 179
XbaI TCTAGA 1 cut(s) 566
XceI RCATGY 2 cut(s) 96, 650
XmaJI CCTAGG 1 cut(s) 167
XmiI GTMKAC 1 cut(s) 516
XspI CTAG 4 cut(s) 50, 168, 420, 567
ZrmI AGTACT 1 cut(s) 722
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.