Rh2DG220500

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0010602
Physical Location & Seq
Forward (+)
21165235 .. 21166124
890 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG220500.1

Sequence Viewer

Length: 390 bp
ATGCTACGATCAGATTTCTTGGCAATCGAAGAAGTAGGAGGTGAGAGTCGGTTTGTTCATTACAGAGTTGATGTAGTGTTTGCTGGCCATGTCCATGCATATGAAAGATCATATAGAATCTCAAATATACACTACAACGTAACAAGTGGTTACCAGTACCCCATACCAGACAAGTCGGCTCCTGTCTACATAACTGTTGAAGATGGAGGGAACCAAGAAGGTCTTGCTGGAAGGTTTAGAGATCCACAACCAGAATATTCTGCATTCCGAGAAGCGAGATATGGGCATTCGACACTGGAGATTCATAACCGGACACATGCACTTTACCACTGGAACCGCAATGATGACGGGAAGAAAATGGCAACTTACGCATTTGTATTGCATAACTAG

Protein Analysis

129

Amino Acids

14.95

Weight (kDa)

6.49

Isoelectric Point (pI)

52.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos_C PF14008 60 - 118 4.4e-19 Iron/zinc purple acid phosphatase-like protein C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 186
AciI CCGC 1 cut(s) 337
AclWI GGATC 1 cut(s) 236
AcoI YGGCCR 1 cut(s) 85
AfaI GTAC 1 cut(s) 158
AgsI TTSAA 1 cut(s) 200
AlwI GGATC 1 cut(s) 236
AoxI GGCC 1 cut(s) 85
AsuHPI GGTGA 1 cut(s) 53
BalI TGGCCA 1 cut(s) 87
BccI CCATC 1 cut(s) 197
BfaI CTAG 1 cut(s) 388
BmiI GGNNCC 3 cut(s) 180, 212, 335
BpmI CTGGAG 1 cut(s) 317
BsaWI WCCGGW 1 cut(s) 309
Bse1I ACTGG 3 cut(s) 154, 300, 335
Bse3DI GCAATG 1 cut(s) 346
BseMI GCAATG 1 cut(s) 346
BseNI ACTGG 3 cut(s) 154, 300, 335
BshFI GGCC 1 cut(s) 87
BsiSI CCGG 1 cut(s) 310
BsmI GAATGC 2 cut(s) 263, 286
BsnI GGCC 1 cut(s) 87
Bsp143I GATC 3 cut(s) 8, 107, 241
BspACI CCGC 1 cut(s) 337
BspANI GGCC 1 cut(s) 87
BspLI GGNNCC 3 cut(s) 180, 212, 335
BspPI GGATC 1 cut(s) 236
BsrDI GCAATG 1 cut(s) 346
BsrI ACTGG 3 cut(s) 154, 300, 335
BssMI GATC 3 cut(s) 8, 107, 241
Bst4CI ACNGT 1 cut(s) 196
BstC8I GCNNGC 1 cut(s) 85
BstEII GGTNACC 1 cut(s) 149
BstKTI GATC 3 cut(s) 11, 110, 244
BstMBI GATC 3 cut(s) 8, 107, 241
BstMWI GCNNNNNNNGC 1 cut(s) 368
BstNSI RCATGY 1 cut(s) 320
BstPI GGTNACC 1 cut(s) 149
BstX2I RGATCY 1 cut(s) 241
BstYI RGATCY 1 cut(s) 241
BsuRI GGCC 1 cut(s) 87
BtsIMutI CAGTG 2 cut(s) 293, 328
Cac8I GCNNGC 1 cut(s) 85
Csp6I GTAC 1 cut(s) 157
CviAII CATG 3 cut(s) 89, 95, 317
CviJI RGCY 2 cut(s) 87, 179
CviKI_1 RGCY 2 cut(s) 87, 179
CviQI GTAC 1 cut(s) 157
DpnI GATC 3 cut(s) 10, 109, 243
DpnII GATC 3 cut(s) 8, 107, 241
EaeI YGGCCR 1 cut(s) 85
Eco91I GGTNACC 1 cut(s) 149
EcoO65I GGTNACC 1 cut(s) 149
EcoT22I ATGCAT 1 cut(s) 100
FaeI CATG 3 cut(s) 92, 98, 320
FalI AAGNNNNNCTT 2 cut(s) 207, 239
FatI CATG 3 cut(s) 88, 94, 316
FauNDI CATATG 1 cut(s) 100
FblI GTMKAC 1 cut(s) 186
FspBI CTAG 1 cut(s) 388
GsuI CTGGAG 1 cut(s) 317
HaeIII GGCC 1 cut(s) 87
HapII CCGG 1 cut(s) 310
Hin1II CATG 3 cut(s) 92, 98, 320
HinfI GANTC 3 cut(s) 46, 117, 301
HpaII CCGG 1 cut(s) 310
HphI GGTGA 1 cut(s) 53
Hpy166II GTNNAC 1 cut(s) 187
Hpy188I TCNGA 2 cut(s) 13, 269
Hpy8I GTNNAC 1 cut(s) 187
HpyAV CCTTC 2 cut(s) 212, 225
HpyCH4III ACNGT 1 cut(s) 196
HpyCH4IV ACGT 1 cut(s) 138
HpyCH4V TGCA 4 cut(s) 98, 263, 320, 382
HpyF10VI GCNNNNNNNGC 1 cut(s) 368
HpySE526I ACGT 1 cut(s) 138
Hsp92II CATG 3 cut(s) 92, 98, 320
Kzo9I GATC 3 cut(s) 8, 107, 241
LmnI GCTCC 1 cut(s) 184
LpnPI CCDG 9 cut(s) 69, 167, 180, 195, 213, 264, 281, 316, 323
MaeI CTAG 1 cut(s) 388
MaeII ACGT 1 cut(s) 138
MaeIII GTNAC 2 cut(s) 139, 149
MalI GATC 3 cut(s) 10, 109, 243
MboI GATC 3 cut(s) 8, 107, 241
MboII GAAGA 3 cut(s) 41, 212, 364
MflI RGATCY 1 cut(s) 241
MlsI TGGCCA 1 cut(s) 87
MluNI TGGCCA 1 cut(s) 87
MlyI GAGTC 1 cut(s) 55
MnlI CCTC 2 cut(s) 32, 200
Mox20I TGGCCA 1 cut(s) 87
Mph1103I ATGCAT 1 cut(s) 100
MscI TGGCCA 1 cut(s) 87
MslI CAYNNNNRTG 2 cut(s) 93, 99
Msp20I TGGCCA 1 cut(s) 87
MspI CCGG 1 cut(s) 310
Mva1269I GAATGC 2 cut(s) 263, 286
MwoI GCNNNNNNNGC 1 cut(s) 368
NdeI CATATG 1 cut(s) 100
NdeII GATC 3 cut(s) 8, 107, 241
NlaIII CATG 3 cut(s) 92, 98, 320
NlaIV GGNNCC 3 cut(s) 180, 212, 335
NsiI ATGCAT 1 cut(s) 100
NspI RCATGY 1 cut(s) 320
PctI GAATGC 2 cut(s) 263, 286
PfeI GAWTC 2 cut(s) 117, 301
PleI GAGTC 1 cut(s) 54
PpsI GAGTC 1 cut(s) 54
PspEI GGTNACC 1 cut(s) 149
PspN4I GGNNCC 3 cut(s) 180, 212, 335
PsuI RGATCY 1 cut(s) 241
RsaI GTAC 1 cut(s) 158
RsaNI GTAC 1 cut(s) 157
RseI CAYNNNNRTG 2 cut(s) 93, 99
Sau3AI GATC 3 cut(s) 8, 107, 241
SchI GAGTC 1 cut(s) 55
SetI ASST 4 cut(s) 43, 141, 223, 236
SmiMI CAYNNNNRTG 2 cut(s) 93, 99
SsiI CCGC 1 cut(s) 337
SspI AATATT 1 cut(s) 257
SspMI CTAG 1 cut(s) 388
TaaI ACNGT 1 cut(s) 196
TaiI ACGT 1 cut(s) 141
TaqI TCGA 2 cut(s) 27, 290
TfiI GAWTC 2 cut(s) 117, 301
TscAI CASTG 2 cut(s) 300, 335
TspDTI ATGAA 3 cut(s) 47, 117, 293
TspRI CASTG 2 cut(s) 300, 335
XceI RCATGY 1 cut(s) 320
XmiI GTMKAC 1 cut(s) 186
XspI CTAG 1 cut(s) 388
Zsp2I ATGCAT 1 cut(s) 100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.