Rmu_sc0002938.1_g000011

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002938.1
OG0008889
Physical Location & Seq
Forward (+)
58391 .. 64903
6513 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4269 bp
atgcttgatacaatgacccctcaacctggacttccatcatcttcatctacttcttactcttcttcttcgtcttccaccgataaccatgagaggatacacgatgtctttttgagttttagaggcgaggacacgcgcgataattttacagaccatttgtacagcgctctgtgtcgaaagggaattaaaaccttcatagataataaacttgacaggggagaagaaatatcagacgcacttctgaaagcaattgaagagtcaaggatctctattgtcgtattctctgaaacttatgcctattcgaagtggtgtttggatgaactcgtgaagattcttcaatgtaaaaagtcaaagaatcagatggttagacccgttttctacaaggtggatccctcaaatgtacggaaccaaagtggtagttttggtgaagcgcttgctcaacatgagaggagaatcaacaataaaatggagagggtcttgtcgtggataaggaatttgctgaactggtcgttgagttatgatgacagcgaattcaagcataacatgaagaagctgaataaatggaccaaagctctcaaggaagcggcggacttgtctggatggacttacttgaatggtttcaggggtaaattggtctctacactatcttactctagccacccatctctctctctctctctcacctggttgcacaatggccggagagaagtcccagcagccgggcaaggtcatctgcaaccatgcatggagctctgcaacaacctcctctcctgccactaccacaccaccattgtcgttccctccactccatcctccgtcatcccctccttttttcccctaaacccacttgccccacagctcctcttccggtcctccctgacccaaacccgacttgctccaccaccaagcagtccaagagctcaaccgccacctcaccaactgcctagccactgctcctcggcctctatgcactgtaatcgacttcaagatcgtaatccacctcgcagctcatcatccaaaaccccaaatattccaattccaattgaatctttcttcttcatcatcaagccaggccgccgagttggccctcttccgcctcgcttaaccctaatttcagagctgcaagaaagagagagtcacagggagttttctgggtttgggtctgaatcaaaatttatcgataacattgttgaagaaatttcagaacaagttttacgtcatagcaattttaatgtggcagatcatccagttggaatacattctcgagtacgagatatgcacaatgttttaggtcttggggtgaaagatgttcgaatggtaggaatatggggtatgggaggaataggcaagacaacaattgctagggctgtttataattcccttgcctataagttcgatggcagttgttttctagaaaatgttggcaacgaatcaatgcagcatggaggcctagtcagactacaaagttaccttctgtacaagattctaggggggcaaaaattagagctgacagatgtgggtgaagggatgactgcgatacaggacaggctacttaaaaaaagggttctcatagtattggatgatgtgaaccaattagaccagttaaaaaaattatgtggaagttttgaatggtttggtattggcagcagaatcatcataacaacaagaaataaacatttgctaactgctcatcaagtcaaacggatacacacgatcaaggagttgaaccatcaggaagctttggagctcttcattgcaaatgccttcccgatagcaaggcttccaagacttccagtcgattattcacaacttgcaagtgatgcagtacattatgctcaaggtctcccatcagctctgatagctttaggttcagttttgtgtggcagacctataatggaatggcaagaggaattaaacactccattttactctcttcaatcaggtcattttgaaactctccgaataagttacaataacttggaagattcggtaaagaatgttttccttgacattgcatttttcttcaacggtcaagatgagaaccatgtgttacagatcctagaaggtggtgacaagcctaacaaaagcattagtgagctcaaagaaaaggcattcataaggattaacgaaaaaaattatatcttgatgcacaatatactagaagtaatggctaaagagatagttcacattgagtcaccaaagaacccagctagacgtagccgattgtggttttctgatgatgtttacgatgttctcacaaaatgcacgatcctagaagatggtgacaagcctaacaaaagcattagtgagctcaaagaaaaggcattcataaggattaacgaaaaaaatcatatcttgatgcacaatatactagaagtaatggctaaagagatagttcacattgagtcaccaaagaacccagctagacgtagccgattgtggttttctgatgatgtttacgatgttctcacaaaatgcacgggaacagacaatatcaaaggcgtcatggtaatgtttggcggaccacgtgagatagcactgcaaaataacagcttcgatgggatgacaaatcttcagttctttataaacaccgatgcacacttttctggagacattgaatatctccccgacaagttaagacttattgattggcctgaatttccctttggatcatttccaatcaattttaatccaactaagcttgttaagcttagtatacctcgtagcctcatctcccaactgggagagacattcaagtctgttggacttcttgataagcttgttattttgagtctttctggatgtgacagactcactaggtttccaagaagaatcgccttgatttcagtaaaatatatcaatctggggggttgcagattgctcgagacattctgcgacattctggaaaatgtggtatccttgacaaggctggatctatccggcacggccattgaagaattgcctgaaacagttatatatctcgtcagcctgaaacatttaattctaagacaatgtgataagcttatccatctgccacatagcatttatgatctgcatagtttatcacgtcttgatctgcagaactgctcaaagcttgtgactctcccaaggtggaatgatgagcagtcagtttcgactaacttgaggtttcttgacctgcgtggttgcacagagcttccagaaattcctgaacttccaccgagcgtggaatggataaatgccgatggctgtgagtcattggtccgagttgcaaaactttcaaacatcttggaacgtagagcttcgcaaatgattgaatcaatttcattgtataattgtcagagattgtgtgacaatctacctcattgcggggcaggtaataagagtagtctcttctctctcattcacttggctaagcagtctgaattcgagattgtatttccagcaagtgaagttcctagttggttcaggaatcgcaagtatttaatggagctttttgatgaacgtgaattttcttttgaaattcctctaaatttcaacaaaggattggctatctgtgcttcagcaactgctgtgggtaatcttgatcagtgctctttcacagccagtattcacatcaatggagaaagcagatgtacttattcattttgtttcgaagcaagcaagatgaaggactctgctcatgtatggctgctctatattccatttgttaagtttgcgtttcatgagccaccttatagctgcctagttaatttgaaacaaactagtaaagattcggtgcgctgtagaagctatggggttcacctggtaatgctacaggatgaatatgaagatttcgtagatgacaccgacttggaagatgagtactcagctggtgaaaatgatgaagacatgatagatcaaattgaggatgtcggccggagcattccgccgggatctgaagaattgcatatctccgatcctcgatttccacgccaaagtcgaatctccattcctgatcaacgcctacccttcacctacaaatcgaaccctaaacaagtgcaatctggactatgtcctcttccagcccaacgccggttcgccggatctggcgatcaatctctactacgacaacatgctcttcgcctaaatcgacgccatctacgctaccctcggatctctaggctacaagaagctttcggttcaccgaggtcgtcgaccgtcccctcgctctcactgatctcacccataccgaataataagaacaccgccactgggtcggattcaggtttggtatcagaaatggagttgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1422

Amino Acids

161.11

Weight (kDa)

6.94

Isoelectric Point (pI)

57.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 33 - 193 1.5e-49 TIR domain
TIR_2 PF13676 35 - 129 2.1e-14 TIR domain
NB-ARC PF00931 434 - 589 3.6e-20 NB-ARC domain
WHD_ROQ1 PF23282 664 - 728 2.6e-06 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 926 - 1068 3.1e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1371, 2582
AasI GACNNNNNNGTC 1 cut(s) 2752
Acc36I ACCTGC 2 cut(s) 3162, 3341
AccI GTMKAC 2 cut(s) 2713, 4172
AccII CGCG 2 cut(s) 133, 135
AciI CCGC 9 cut(s) 581, 584, 923, 1072, 1091, 2517, 3345, 3905, 4224
AcoI YGGCCR 3 cut(s) 694, 2943, 3892
AcuI CTGAAG 3 cut(s) 2555, 3522, 3936
AcvI CACGTG 1 cut(s) 2525
AcyI GRCGYC 2 cut(s) 2499, 4111
AfaI GTAC 7 cut(s) 158, 399, 1266, 1475, 1824, 3613, 3842
AfeI AGCGCT 2 cut(s) 163, 429
AfiI CCNNNNNNNGG 6 cut(s) 26, 716, 2922, 3344, 4050, 4230
AhdI GACNNNNNGTC 1 cut(s) 1790
AhlI ACTAGT 1 cut(s) 3740
AjiI CACGTC 1 cut(s) 3065
AjnI CCWGG 4 cut(s) 25, 680, 1066, 3780
AjuI GAANNNNNNNTTGG 4 cut(s) 293, 325, 2646, 2678
Alw21I GWGCWC 6 cut(s) 750, 919, 1746, 2097, 2310, 3572
Alw26I GTCTC 6 cut(s) 637, 1844, 2601, 2738, 2876, 3371
AlwNI CAGNNNCTG 1 cut(s) 3545
Ama87I CYCGRG 2 cut(s) 1260, 2879
Aor51HI AGCGCT 2 cut(s) 163, 429
AoxI GGCC 8 cut(s) 694, 957, 1069, 1081, 1444, 2648, 2943, 3892
ApeKI GCWGC 7 cut(s) 713, 1002, 1117, 1435, 1641, 3667, 3717
Asp700I GAANNNNTTC 3 cut(s) 614, 1255, 1997
AspLEI GCGC 4 cut(s) 135, 164, 430, 3759
AspS9I GGNCC 5 cut(s) 561, 867, 1082, 2519, 3238
AsuC2I CCSGG 2 cut(s) 718, 3909
AsuII TTCGAA 3 cut(s) 299, 1309, 3630
AvaI CYCGRG 2 cut(s) 1260, 2879
AvaII GGWCC 4 cut(s) 561, 867, 2519, 3238
BamHI GGATCC 1 cut(s) 385
BanII GRGCYC 5 cut(s) 750, 919, 1746, 2097, 2310
BauI CACGAG 1 cut(s) 320
BbrPI CACGTG 1 cut(s) 2525
BbsI GAAGAC 2 cut(s) 63, 3870
Bbv12I GWGCWC 6 cut(s) 750, 919, 1746, 2097, 2310, 3572
BbvI GCAGC 7 cut(s) 725, 1014, 1104, 1447, 1653, 3654, 3704
BceAI ACGGC 1 cut(s) 2958
BcgI CGANNNNNNTGC 4 cut(s) 956, 990, 2860, 2894
BciT130I CCWGG 4 cut(s) 27, 682, 1068, 3782
BciVI GTATCC 3 cut(s) 87, 1695, 2923
BclI TGATCA 2 cut(s) 3562, 3973
BcnI CCSGG 2 cut(s) 718, 3909
BcoDI GTCTC 6 cut(s) 637, 1844, 2601, 2738, 2876, 3371
BcuI ACTAGT 1 cut(s) 3740
BfmI CTRYAG 3 cut(s) 3074, 3760, 3791
BfoI RGCGCY 2 cut(s) 165, 431
BfuAI ACCTGC 2 cut(s) 3162, 3341
BfuI GTATCC 3 cut(s) 87, 1695, 2923
BglI GCCNNNNNGGC 1 cut(s) 1080
BisI GCNGC 9 cut(s) 582, 714, 1003, 1072, 1118, 1436, 1642, 3668, 3718
BlpI GCTNAGC 1 cut(s) 3390
BlsI GCNGC 9 cut(s) 583, 715, 1004, 1073, 1119, 1437, 1643, 3669, 3719
BmcAI AGTACT 1 cut(s) 3842
Bme1390I CCNGG 6 cut(s) 27, 682, 718, 1068, 3782, 3909
Bme18I GGWCC 4 cut(s) 561, 867, 2519, 3238
BmeRI GACNNNNNGTC 1 cut(s) 1790
BmeT110I CYCGRG 2 cut(s) 1260, 2879
BmgBI CACGTC 1 cut(s) 3065
BmgT120I GGNCC 5 cut(s) 561, 867, 1082, 2519, 3238
BmiI GGNNCC 2 cut(s) 387, 404
BmrFI CCNGG 6 cut(s) 27, 682, 718, 1068, 3782, 3909
BmrI ACTGGG 2 cut(s) 2747, 4239
BmsI GCATC 4 cut(s) 1807, 2133, 2346, 2581
BmuI ACTGGG 2 cut(s) 2747, 4239
BpiI GAAGAC 2 cut(s) 63, 3870
BpmI CTGGAG 1 cut(s) 2625
Bpu1102I GCTNAGC 1 cut(s) 3390
Bpu14I TTCGAA 3 cut(s) 299, 1309, 3630
BpuEI CTTGAG 3 cut(s) 557, 1818, 3160
BpuMI CCSGG 2 cut(s) 718, 3909
Bsa29I ATCGAT 1 cut(s) 1176
BsaAI YACGTR 1 cut(s) 2525
BsaBI GATNNNNATC 1 cut(s) 990
BsaHI GRCGYC 2 cut(s) 2499, 4111
BsaI GGTCTC 2 cut(s) 637, 1844
BsaJI CCNNGG 4 cut(s) 954, 3104, 4127, 4163
BsaWI WCCGGW 1 cut(s) 864
BsaXI ACNNNNNCTCC 4 cut(s) 749, 779, 1434, 1464
Bsc4I CCNNNNNNNGG 6 cut(s) 26, 716, 2922, 3344, 4050, 4230
Bse118I RCCGGY 1 cut(s) 4050
Bse1I ACTGG 7 cut(s) 506, 1244, 1597, 1790, 2742, 3582, 4234
Bse3DI GCAATG 3 cut(s) 1749, 2007, 3340
Bse8I GATNNNNATC 1 cut(s) 990
BseBI CCWGG 4 cut(s) 27, 682, 1068, 3782
BseCI ATCGAT 1 cut(s) 1176
BseDI CCNNGG 4 cut(s) 954, 3104, 4127, 4163
BseJI GATNNNNATC 1 cut(s) 990
BseLI CCNNNNNNNGG 6 cut(s) 26, 716, 2922, 3344, 4050, 4230
BseMI GCAATG 3 cut(s) 1749, 2007, 3340
BseMII CTCAG 1 cut(s) 3858
BseNI ACTGG 7 cut(s) 506, 1244, 1597, 1790, 2742, 3582, 4234
BseRI GAGGAG 4 cut(s) 460, 752, 848, 943
BseX3I CGGCCG 1 cut(s) 3892
BseXI GCAGC 7 cut(s) 725, 1014, 1104, 1447, 1653, 3654, 3704
BseYI CCCAGC 3 cut(s) 709, 2203, 2416
Bsh1236I CGCG 2 cut(s) 133, 135
Bsh1285I CGRYCG 2 cut(s) 3895, 4176
BshFI GGCC 8 cut(s) 696, 959, 1071, 1083, 1446, 2650, 2945, 3894
BshVI ATCGAT 1 cut(s) 1176
BsiEI CGRYCG 2 cut(s) 3895, 4176
BsiHKAI GWGCWC 6 cut(s) 750, 919, 1746, 2097, 2310, 3572
BsiHKCI CYCGRG 2 cut(s) 1260, 2879
BsiSI CCGG 8 cut(s) 697, 717, 865, 2937, 3895, 3908, 4051, 4059
BslFI GGGAC 2 cut(s) 692, 4163
BslI CCNNNNNNNGG 6 cut(s) 26, 716, 2922, 3344, 4050, 4230
BsmAI GTCTC 6 cut(s) 637, 1844, 2601, 2738, 2876, 3371
BsmFI GGGAC 2 cut(s) 692, 4163
BsmI GAATGC 3 cut(s) 2108, 2321, 3900
BsnI GGCC 8 cut(s) 696, 959, 1071, 1083, 1446, 2650, 2945, 3894
Bso31I GGTCTC 2 cut(s) 637, 1844
BsoBI CYCGRG 2 cut(s) 1260, 2879
Bsp119I TTCGAA 3 cut(s) 299, 1309, 3630
Bsp1286I GDGCHC 6 cut(s) 750, 919, 1746, 2097, 2310, 3572
Bsp1407I TGTACA 2 cut(s) 156, 1473
Bsp1720I GCTNAGC 1 cut(s) 3390
BspACI CCGC 9 cut(s) 581, 584, 923, 1072, 1091, 2517, 3345, 3905, 4224
BspANI GGCC 8 cut(s) 696, 959, 1071, 1083, 1446, 2650, 2945, 3894
BspCNI CTCAG 1 cut(s) 3857
BspDI ATCGAT 1 cut(s) 1176
BspFNI CGCG 2 cut(s) 133, 135
BspHI TCATGA 1 cut(s) 3700
BspLI GGNNCC 2 cut(s) 387, 404
BspMAI CTGCAG 1 cut(s) 3078
BspMI ACCTGC 2 cut(s) 3162, 3341
BspQI GCTCTTC 2 cut(s) 1751, 4101
BspT104I TTCGAA 3 cut(s) 299, 1309, 3630
BspTNI GGTCTC 2 cut(s) 637, 1844
BsrDI GCAATG 3 cut(s) 1749, 2007, 3340
BsrFI RCCGGY 1 cut(s) 4050
BsrGI TGTACA 2 cut(s) 156, 1473
BsrI ACTGG 7 cut(s) 506, 1244, 1597, 1790, 2742, 3582, 4234
BssAI RCCGGY 1 cut(s) 4050
BssECI CCNNGG 4 cut(s) 954, 3104, 4127, 4163
BssNAI GTATAC 1 cut(s) 2714
BssNI GRCGYC 2 cut(s) 2499, 4111
BssSI CACGAG 1 cut(s) 320
BssT1I CCWWGG 1 cut(s) 3104
Bst1107I GTATAC 1 cut(s) 2714
Bst2BI CACGAG 1 cut(s) 320
Bst2UI CCWGG 4 cut(s) 27, 682, 1068, 3782
Bst4CI ACNGT 4 cut(s) 971, 2027, 2968, 4177
Bst6I CTCTTC 9 cut(s) 64, 246, 866, 1092, 1751, 1935, 3374, 4041, 4101
BstACI GRCGYC 2 cut(s) 2499, 4111
BstAPI GCANNNNNTGC 1 cut(s) 1817
BstAUI TGTACA 2 cut(s) 156, 1473
BstBAI YACGTR 1 cut(s) 2525
BstBI TTCGAA 3 cut(s) 299, 1309, 3630
BstC8I GCNNGC 2 cut(s) 432, 3637
BstDEI CTNAG 5 cut(s) 2694, 2708, 3002, 3390, 3844
BstENI CCTNNNNNAGG 1 cut(s) 2920
BstFNI CGCG 2 cut(s) 133, 135
BstH2I RGCGCY 2 cut(s) 165, 431
BstHHI GCGC 4 cut(s) 135, 164, 430, 3759
BstMAI GTCTC 6 cut(s) 637, 1844, 2601, 2738, 2876, 3371
BstMCI CGRYCG 2 cut(s) 3895, 4176
BstMWI GCNNNNNNNGC 7 cut(s) 1080, 1817, 1856, 2704, 3533, 3765, 4119
BstNI CCWGG 4 cut(s) 27, 682, 1068, 3782
BstNSI RCATGY 1 cut(s) 4094
BstSCI CCNGG 6 cut(s) 25, 680, 716, 1066, 3780, 3907
BstSFI CTRYAG 3 cut(s) 3074, 3760, 3791
BstUI CGCG 2 cut(s) 133, 135
BstV1I GCAGC 7 cut(s) 725, 1014, 1104, 1447, 1653, 3654, 3704
BstV2I GAAGAC 2 cut(s) 63, 3870
BstX2I RGATCY 7 cut(s) 261, 385, 2052, 2929, 3911, 4061, 4131
BstYI RGATCY 7 cut(s) 261, 385, 2052, 2929, 3911, 4061, 4131
BstZ17I GTATAC 1 cut(s) 2714
BstZI CGGCCG 1 cut(s) 3892
Bsu15I ATCGAT 1 cut(s) 1176
BsuI GTATCC 3 cut(s) 87, 1695, 2923
BsuRI GGCC 8 cut(s) 696, 959, 1071, 1083, 1446, 2650, 2945, 3894
BsuTUI ATCGAT 1 cut(s) 1176
BtrI CACGTC 1 cut(s) 3065
BtsI GCAGTG 2 cut(s) 946, 2534
BtsIMutI CAGTG 6 cut(s) 946, 967, 2534, 3572, 4190, 4227
BveI ACCTGC 2 cut(s) 3162, 3341
Cac8I GCNNGC 2 cut(s) 432, 3637
CaiI CAGNNNCTG 1 cut(s) 3545
CciI TCATGA 1 cut(s) 3700
CfoI GCGC 4 cut(s) 135, 164, 430, 3759
Cfr10I RCCGGY 1 cut(s) 4050
Cfr13I GGNCC 5 cut(s) 561, 867, 1082, 2519, 3238
ClaI ATCGAT 1 cut(s) 1176
CseI GACGC 3 cut(s) 239, 2488, 4119
CsiI ACCWGGT 2 cut(s) 680, 3780
Csp6I GTAC 7 cut(s) 157, 398, 1265, 1474, 1823, 3612, 3841
CspCI CAANNNNNGTGG 2 cut(s) 3531, 3566
CviQI GTAC 7 cut(s) 157, 398, 1265, 1474, 1823, 3612, 3841
DdeI CTNAG 5 cut(s) 2694, 2708, 3002, 3390, 3844
DrdI GACNNNNNNGTC 1 cut(s) 2752
DriI GACNNNNNGTC 1 cut(s) 1790
DseDI GACNNNNNNGTC 1 cut(s) 2752
EaeI YGGCCR 3 cut(s) 694, 2943, 3892
EagI CGGCCG 1 cut(s) 3892
Eam1104I CTCTTC 9 cut(s) 64, 246, 866, 1092, 1751, 1935, 3374, 4041, 4101
Eam1105I GACNNNNNGTC 1 cut(s) 1790
EarI CTCTTC 9 cut(s) 64, 246, 866, 1092, 1751, 1935, 3374, 4041, 4101
EciI GGCGGA 4 cut(s) 599, 1080, 2532, 3894
Ecl136II GAGCTC 5 cut(s) 748, 917, 1744, 2095, 2308
EclXI CGGCCG 1 cut(s) 3892
Eco130I CCWWGG 1 cut(s) 3104
Eco147I AGGCCT 1 cut(s) 1446
Eco24I GRGCYC 5 cut(s) 750, 919, 1746, 2097, 2310
Eco31I GGTCTC 2 cut(s) 637, 1844
Eco47I GGWCC 4 cut(s) 561, 867, 2519, 3238
Eco47III AGCGCT 2 cut(s) 163, 429
Eco52I CGGCCG 1 cut(s) 3892
Eco53kI GAGCTC 5 cut(s) 748, 917, 1744, 2095, 2308
Eco57I CTGAAG 3 cut(s) 2555, 3522, 3936
Eco72I CACGTG 1 cut(s) 2525
Eco88I CYCGRG 2 cut(s) 1260, 2879
EcoICRI GAGCTC 5 cut(s) 748, 917, 1744, 2095, 2308
EcoNI CCTNNNNNAGG 1 cut(s) 2920
EcoRI GAATTC 2 cut(s) 527, 3401
EcoRII CCWGG 4 cut(s) 25, 680, 1066, 3780
EcoT14I CCWWGG 1 cut(s) 3104
EcoT22I ATGCAT 1 cut(s) 743
EcoT38I GRGCYC 5 cut(s) 750, 919, 1746, 2097, 2310
ErhI CCWWGG 1 cut(s) 3104
FaqI GGGAC 2 cut(s) 692, 4163
FauI CCCGC 1 cut(s) 3338
FbaI TGATCA 2 cut(s) 3562, 3973
FblI GTMKAC 2 cut(s) 2713, 4172
Fnu4HI GCNGC 9 cut(s) 582, 714, 1003, 1072, 1118, 1436, 1642, 3668, 3718
FriOI GRGCYC 5 cut(s) 750, 919, 1746, 2097, 2310
Fsp4HI GCNGC 9 cut(s) 582, 714, 1003, 1072, 1118, 1436, 1642, 3668, 3718
GlaI GCGC 4 cut(s) 134, 163, 429, 3758
GluI GCNGC 9 cut(s) 582, 714, 1003, 1072, 1118, 1436, 1642, 3668, 3718
GsaI CCCAGC 3 cut(s) 713, 2207, 2420
GsuI CTGGAG 1 cut(s) 2625
HaeII RGCGCY 2 cut(s) 165, 431
HaeIII GGCC 8 cut(s) 696, 959, 1071, 1083, 1446, 2650, 2945, 3894
HapII CCGG 8 cut(s) 697, 717, 865, 2937, 3895, 3908, 4051, 4059
HgaI GACGC 3 cut(s) 239, 2488, 4119
HhaI GCGC 4 cut(s) 135, 164, 430, 3759
Hin1I GRCGYC 2 cut(s) 2499, 4111
Hin6I GCGC 4 cut(s) 133, 162, 428, 3757
HinP1I GCGC 4 cut(s) 133, 162, 428, 3757
HincII GTYRAC 1 cut(s) 4173
HindII GTYRAC 1 cut(s) 4173
HindIII AAGCTT 7 cut(s) 1734, 2696, 2705, 2774, 3017, 3089, 4149
HpaII CCGG 8 cut(s) 697, 717, 865, 2937, 3895, 3908, 4051, 4059
Hpy166II GTNNAC 9 cut(s) 1585, 2182, 2242, 2395, 2455, 2714, 3778, 4160, 4173
Hpy8I GTNNAC 9 cut(s) 1585, 2182, 2242, 2395, 2455, 2714, 3778, 4160, 4173
Hpy99I CGWCG 2 cut(s) 4113, 4174
HpyAV CCTTC 7 cut(s) 199, 1478, 1514, 1771, 2054, 3640, 3997
HpyCH4III ACNGT 4 cut(s) 971, 2027, 2968, 4177
HpyCH4IV ACGT 7 cut(s) 1213, 2212, 2425, 2524, 3064, 3271, 3481
HpyF10VI GCNNNNNNNGC 7 cut(s) 1080, 1817, 1856, 2704, 3533, 3765, 4119
HpyF3I CTNAG 5 cut(s) 2694, 2708, 3002, 3390, 3844
HpySE526I ACGT 7 cut(s) 1213, 2212, 2425, 2524, 3064, 3271, 3481
Hsp92I GRCGYC 2 cut(s) 2499, 4111
HspAI GCGC 4 cut(s) 133, 162, 428, 3757
Ksp22I TGATCA 2 cut(s) 3562, 3973
LguI GCTCTTC 2 cut(s) 1751, 4101
LmnI GCTCC 7 cut(s) 745, 861, 898, 956, 1741, 3466, 3897
Lsp1109I GCAGC 7 cut(s) 725, 1014, 1104, 1447, 1653, 3654, 3704
LweI GCATC 4 cut(s) 1807, 2133, 2346, 2581
MabI ACCWGGT 2 cut(s) 680, 3780
MaeII ACGT 7 cut(s) 1213, 2212, 2425, 2524, 3064, 3271, 3481
MfeI CAATTG 3 cut(s) 246, 1038, 1353
MflI RGATCY 7 cut(s) 261, 385, 2052, 2929, 3911, 4061, 4131
MhlI GDGCHC 6 cut(s) 750, 919, 1746, 2097, 2310, 3572
MlyI GAGTC 9 cut(s) 263, 1141, 2198, 2411, 2797, 2802, 3091, 3239, 3644
MmeI TCCRAC 4 cut(s) 1228, 2714, 2740, 4215
Mph1103I ATGCAT 1 cut(s) 743
MroXI GAANNNNTTC 3 cut(s) 614, 1255, 1997
MslI CAYNNNNRTG 2 cut(s) 2507, 3594
MspA1I CMGCKG 1 cut(s) 3848
MspI CCGG 8 cut(s) 697, 717, 865, 2937, 3895, 3908, 4051, 4059
MspR9I CCNGG 6 cut(s) 27, 682, 718, 1068, 3782, 3909
MunI CAATTG 3 cut(s) 246, 1038, 1353
Mva1269I GAATGC 3 cut(s) 2108, 2321, 3900
MvaI CCWGG 4 cut(s) 27, 682, 1068, 3782
MvnI CGCG 2 cut(s) 133, 135
MwoI GCNNNNNNNGC 7 cut(s) 1080, 1817, 1856, 2704, 3533, 3765, 4119
NciI CCSGG 2 cut(s) 718, 3909
NlaIV GGNNCC 2 cut(s) 387, 404
NmeAIII GCCGAG 2 cut(s) 935, 1100
NmuCI GTSAC 8 cut(s) 1133, 2066, 2190, 2279, 2403, 2801, 3094, 3326
NsiI ATGCAT 1 cut(s) 743
NspI RCATGY 1 cut(s) 4094
NspV TTCGAA 3 cut(s) 299, 1309, 3630
PaeR7I CTCGAG 2 cut(s) 1260, 2879
PagI TCATGA 1 cut(s) 3700
PceI AGGCCT 1 cut(s) 1446
PciSI GCTCTTC 2 cut(s) 1751, 4101
PcsI WCGNNNNNNNCGW 2 cut(s) 3955, 4105
PctI GAATGC 3 cut(s) 2108, 2321, 3900
PdmI GAANNNNTTC 3 cut(s) 614, 1255, 1997
PflFI GACNNNGTC 1 cut(s) 4029
PkrI GCNGC 9 cut(s) 583, 715, 1004, 1073, 1119, 1437, 1643, 3669, 3719
PleI GAGTC 9 cut(s) 262, 1140, 2197, 2410, 2796, 2802, 3091, 3238, 3644
PmaCI CACGTG 1 cut(s) 2525
PmlI CACGTG 1 cut(s) 2525
PpsI GAGTC 9 cut(s) 262, 1140, 2197, 2410, 2796, 2802, 3091, 3238, 3644
Ppu21I YACGTR 1 cut(s) 2525
PsiI TTATAA 2 cut(s) 1371, 2582
Psp124BI GAGCTC 5 cut(s) 750, 919, 1746, 2097, 2310
Psp6I CCWGG 4 cut(s) 25, 680, 1066, 3780
PspCI CACGTG 1 cut(s) 2525
PspFI CCCAGC 3 cut(s) 709, 2203, 2416
PspGI CCWGG 4 cut(s) 25, 680, 1066, 3780
PspN4I GGNNCC 2 cut(s) 387, 404
PspPI GGNCC 5 cut(s) 561, 867, 1082, 2519, 3238
PsrI GAACNNNNNNTAC 2 cut(s) 1194, 1226
PstI CTGCAG 1 cut(s) 3078
PstNI CAGNNNCTG 1 cut(s) 3545
PsuI RGATCY 7 cut(s) 261, 385, 2052, 2929, 3911, 4061, 4131
PsyI GACNNNGTC 1 cut(s) 4029
PvuII CAGCTG 1 cut(s) 3848
RsaI GTAC 7 cut(s) 158, 399, 1266, 1475, 1824, 3613, 3842
RsaNI GTAC 7 cut(s) 157, 398, 1265, 1474, 1823, 3612, 3841
RseI CAYNNNNRTG 2 cut(s) 2507, 3594
SacI GAGCTC 5 cut(s) 750, 919, 1746, 2097, 2310
SalI GTCGAC 1 cut(s) 4171
SapI GCTCTTC 2 cut(s) 1751, 4101
SatI GCNGC 9 cut(s) 582, 714, 1003, 1072, 1118, 1436, 1642, 3668, 3718
Sau96I GGNCC 5 cut(s) 561, 867, 1082, 2519, 3238
ScaI AGTACT 1 cut(s) 3842
SchI GAGTC 9 cut(s) 263, 1141, 2198, 2411, 2797, 2802, 3091, 3239, 3644
ScrFI CCNGG 6 cut(s) 27, 682, 718, 1068, 3782, 3909
SduI GDGCHC 6 cut(s) 750, 919, 1746, 2097, 2310, 3572
SexAI ACCWGGT 2 cut(s) 680, 3780
SfaNI GCATC 4 cut(s) 1807, 2133, 2346, 2581
SfcI CTRYAG 3 cut(s) 3074, 3760, 3791
Sfr274I CTCGAG 2 cut(s) 1260, 2879
SfuI TTCGAA 3 cut(s) 299, 1309, 3630
SinI GGWCC 4 cut(s) 561, 867, 2519, 3238
SlaI CTCGAG 2 cut(s) 1260, 2879
SmiMI CAYNNNNRTG 2 cut(s) 2507, 3594
SmlI CTYRAG 5 cut(s) 572, 1260, 1833, 2879, 3139
SmoI CTYRAG 5 cut(s) 572, 1260, 1833, 2879, 3139
SpeI ACTAGT 1 cut(s) 3740
SseBI AGGCCT 1 cut(s) 1446
SsiI CCGC 9 cut(s) 581, 584, 923, 1072, 1091, 2517, 3345, 3905, 4224
SspI AATATT 1 cut(s) 1027
SstI GAGCTC 5 cut(s) 750, 919, 1746, 2097, 2310
StuI AGGCCT 1 cut(s) 1446
StyD4I CCNGG 6 cut(s) 25, 680, 716, 1066, 3780, 3907
StyI CCWWGG 1 cut(s) 3104
TaaI ACNGT 4 cut(s) 971, 2027, 2968, 4177
TaiI ACGT 7 cut(s) 1216, 2215, 2428, 2527, 3067, 3274, 3484
TatI WGTACW 5 cut(s) 156, 1473, 1822, 3611, 3840
TauI GCSGC 2 cut(s) 584, 1074
TscAI CASTG 6 cut(s) 953, 974, 2541, 3572, 4197, 4234
TseFI GTSAC 8 cut(s) 1133, 2066, 2190, 2279, 2403, 2801, 3094, 3326
TseI GCWGC 7 cut(s) 713, 1002, 1117, 1435, 1641, 3667, 3717
Tsp45I GTSAC 8 cut(s) 1133, 2066, 2190, 2279, 2403, 2801, 3094, 3326
TspGWI ACGGA 3 cut(s) 415, 802, 1714
TspRI CASTG 6 cut(s) 953, 974, 2541, 3572, 4197, 4234
Tth111I GACNNNGTC 1 cut(s) 4029
VpaK11BI GGWCC 4 cut(s) 561, 867, 2519, 3238
XagI CCTNNNNNAGG 1 cut(s) 2920
XbaI TCTAGA 1 cut(s) 1408
XceI RCATGY 1 cut(s) 4094
XhoI CTCGAG 2 cut(s) 1260, 2879
XmiI GTMKAC 2 cut(s) 2713, 4172
XmnI GAANNNNTTC 3 cut(s) 614, 1255, 1997
ZrmI AGTACT 1 cut(s) 3842
Zsp2I ATGCAT 1 cut(s) 743
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.