Rmu_sc0003058.1_g000007

Domain of unknown function (DUF4283)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003058.1
OG0011603
Physical Location & Seq
Forward (+)
34004 .. 34753
750 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 750 bp
atggctggaggcatgaatagggatcacgaggtgattgtcaattcagattcgacgctggtttccttgggggacgcgagagctgctgcgttccaggacggcttctggtatatggctggtaggctgctggcaccaaaagtcaagttcccgggtttcaaagggaccattgccactatttggcgtgtcagatcgggtctcactatacaagatgccggagagcgttttgtttttcagttcgacagtgaagcggtgcgtaatagcatcttgcatgggggtccttggttctaccacaataccatgtctcggaaggaggttgtgaagttctcctcgatgcaagtggtgccactggggaggaaaaggaaggctgttcccaagttagatcggttcgggaaaaagtacttggcttttcctgattcgatacttgaggttgaagataacgtctcgctggtgctgcaacaaaagcatggcaagatatgggtctcgcctaagaaaaagaaagttgggcatcccaagggaagtaagaatagagtgaagtctgctgtggaacaggatgacaaaactatacgtaagtataaacgtcggggtaaagctttgtgctttgactctaatctggaagataatgtccatgtcaaagttggcaaaccagtgacggtgttggaggagacgaatgtgactactcctgcattggaacttgcagctgtggaggtaccggtggaatgtgagagctcacattctaccactattttagtttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 zf-CCHC_4

Protein Analysis

249

Amino Acids

27.79

Weight (kDa)

9.74

Isoelectric Point (pI)

42.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 52 - 99 1e-05 Domain of unknown function (DUF4283)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 703
AccB1I GGYRCC 3 cut(s) 127, 337, 703
AccB7I CCANNNNNTGG 1 cut(s) 174
AccII CGCG 1 cut(s) 74
AciI CCGC 1 cut(s) 245
AclWI GGATC 1 cut(s) 30
AdeI CACNNNGTG 1 cut(s) 31
AfaI GTAC 2 cut(s) 395, 705
AfiI CCNNNNNNNGG 2 cut(s) 174, 300
AgeI ACCGGT 1 cut(s) 706
AgsI TTSAA 2 cut(s) 154, 428
AjnI CCWGG 1 cut(s) 90
AjuI GAANNNNNNNTTGG 2 cut(s) 380, 412
AluBI AGCT 4 cut(s) 80, 587, 695, 723
AluI AGCT 4 cut(s) 80, 587, 695, 723
Alw21I GWGCWC 1 cut(s) 725
Alw26I GTCTC 5 cut(s) 197, 303, 442, 481, 653
AlwI GGATC 1 cut(s) 30
Ama87I CYCGRG 1 cut(s) 145
ApeKI GCWGC 5 cut(s) 80, 83, 121, 448, 692
AsiGI ACCGGT 1 cut(s) 706
Asp718I GGTACC 1 cut(s) 703
AspS9I GGNCC 2 cut(s) 159, 272
AsuC2I CCSGG 2 cut(s) 146, 147
AsuHPI GGTGA 1 cut(s) 43
AvaI CYCGRG 1 cut(s) 145
AvaII GGWCC 2 cut(s) 159, 272
BanI GGYRCC 3 cut(s) 127, 337, 703
BanII GRGCYC 1 cut(s) 725
BauI CACGAG 1 cut(s) 26
Bbv12I GWGCWC 1 cut(s) 725
BbvI GCAGC 5 cut(s) 67, 70, 108, 435, 704
BceAI ACGGC 1 cut(s) 112
BciT130I CCWGG 1 cut(s) 92
BcnI CCSGG 2 cut(s) 146, 147
BcoDI GTCTC 5 cut(s) 197, 303, 442, 481, 653
BisI GCNGC 5 cut(s) 81, 84, 122, 449, 693
BlsI GCNGC 5 cut(s) 82, 85, 123, 450, 694
BmcAI AGTACT 1 cut(s) 395
Bme1390I CCNGG 3 cut(s) 92, 146, 147
Bme18I GGWCC 2 cut(s) 159, 272
BmeT110I CYCGRG 1 cut(s) 145
BmgT120I GGNCC 2 cut(s) 159, 272
BmiI GGNNCC 5 cut(s) 129, 160, 273, 339, 705
BmrFI CCNGG 3 cut(s) 92, 146, 147
BmrI ACTGGG 1 cut(s) 353
BmsI GCATC 4 cut(s) 196, 267, 318, 511
BmuI ACTGGG 1 cut(s) 353
BpmI CTGGAG 1 cut(s) 27
BpuEI CTTGAG 1 cut(s) 440
BpuMI CCSGG 2 cut(s) 146, 147
BsaAI YACGTR 1 cut(s) 563
BsaI GGTCTC 2 cut(s) 197, 481
BsaJI CCNNGG 4 cut(s) 63, 145, 275, 507
BsaWI WCCGGW 1 cut(s) 706
Bsc4I CCNNNNNNNGG 2 cut(s) 174, 300
Bse118I RCCGGY 1 cut(s) 706
Bse1I ACTGG 2 cut(s) 348, 641
Bse3DI GCAATG 1 cut(s) 162
BseBI CCWGG 1 cut(s) 92
BseDI CCNNGG 4 cut(s) 63, 145, 275, 507
BseGI GGATG 2 cut(s) 502, 553
BseLI CCNNNNNNNGG 2 cut(s) 174, 300
BseMI GCAATG 1 cut(s) 162
BseNI ACTGG 2 cut(s) 348, 641
BseRI GAGGAG 2 cut(s) 313, 671
BseXI GCAGC 5 cut(s) 67, 70, 108, 435, 704
Bsh1236I CGCG 1 cut(s) 74
BshNI GGYRCC 3 cut(s) 127, 337, 703
BshTI ACCGGT 1 cut(s) 706
BsiHKAI GWGCWC 1 cut(s) 725
BsiHKCI CYCGRG 1 cut(s) 145
BsiSI CCGG 3 cut(s) 146, 210, 707
BslFI GGGAC 2 cut(s) 83, 172
BslI CCNNNNNNNGG 2 cut(s) 174, 300
BsmAI GTCTC 5 cut(s) 197, 303, 442, 481, 653
BsmBI CGTCTC 2 cut(s) 442, 653
BsmFI GGGAC 2 cut(s) 83, 172
Bso31I GGTCTC 2 cut(s) 197, 481
BsoBI CYCGRG 1 cut(s) 145
Bsp1286I GDGCHC 1 cut(s) 725
Bsp143I GATC 3 cut(s) 22, 185, 376
BspACI CCGC 1 cut(s) 245
BspFNI CGCG 1 cut(s) 74
BspLI GGNNCC 5 cut(s) 129, 160, 273, 339, 705
BspPI GGATC 1 cut(s) 30
BspT107I GGYRCC 3 cut(s) 127, 337, 703
BspTNI GGTCTC 2 cut(s) 197, 481
BsrDI GCAATG 1 cut(s) 162
BsrFI RCCGGY 1 cut(s) 706
BsrI ACTGG 2 cut(s) 348, 641
BssAI RCCGGY 1 cut(s) 706
BssECI CCNNGG 4 cut(s) 63, 145, 275, 507
BssMI GATC 3 cut(s) 22, 185, 376
BssSI CACGAG 1 cut(s) 26
BssT1I CCWWGG 3 cut(s) 63, 275, 507
Bst2BI CACGAG 1 cut(s) 26
Bst2UI CCWGG 1 cut(s) 92
Bst4CI ACNGT 2 cut(s) 239, 649
BstAPI GCANNNNNTGC 1 cut(s) 337
BstBAI YACGTR 1 cut(s) 563
BstC8I GCNNGC 1 cut(s) 126
BstDEI CTNAG 1 cut(s) 483
BstF5I GGATG 2 cut(s) 502, 553
BstFNI CGCG 1 cut(s) 74
BstKTI GATC 3 cut(s) 25, 188, 379
BstMAI GTCTC 5 cut(s) 197, 303, 442, 481, 653
BstMBI GATC 3 cut(s) 22, 185, 376
BstMWI GCNNNNNNNGC 4 cut(s) 80, 337, 448, 457
BstNI CCWGG 1 cut(s) 92
BstSCI CCNGG 3 cut(s) 90, 144, 145
BstSNI TACGTA 1 cut(s) 563
BstUI CGCG 1 cut(s) 74
BstV1I GCAGC 5 cut(s) 67, 70, 108, 435, 704
BtsCI GGATG 2 cut(s) 502, 553
BtsIMutI CAGTG 3 cut(s) 244, 341, 648
Cac8I GCNNGC 1 cut(s) 126
Cfr10I RCCGGY 1 cut(s) 706
Cfr13I GGNCC 2 cut(s) 159, 272
Cfr9I CCCGGG 1 cut(s) 145
CseI GACGC 2 cut(s) 61, 80
Csp6I GTAC 2 cut(s) 394, 704
CspAI ACCGGT 1 cut(s) 706
CviAII CATG 5 cut(s) 13, 266, 295, 461, 623
CviQI GTAC 2 cut(s) 394, 704
DdeI CTNAG 1 cut(s) 483
DpnI GATC 3 cut(s) 24, 187, 378
DpnII GATC 3 cut(s) 22, 185, 376
DraIII CACNNNGTG 1 cut(s) 31
Ecl136II GAGCTC 1 cut(s) 723
Eco105I TACGTA 1 cut(s) 563
Eco130I CCWWGG 3 cut(s) 63, 275, 507
Eco24I GRGCYC 1 cut(s) 725
Eco31I GGTCTC 2 cut(s) 197, 481
Eco47I GGWCC 2 cut(s) 159, 272
Eco53kI GAGCTC 1 cut(s) 723
Eco88I CYCGRG 1 cut(s) 145
EcoICRI GAGCTC 1 cut(s) 723
EcoO109I RGGNCCY 1 cut(s) 272
EcoRII CCWGG 1 cut(s) 90
EcoT14I CCWWGG 3 cut(s) 63, 275, 507
EcoT38I GRGCYC 1 cut(s) 725
ErhI CCWWGG 3 cut(s) 63, 275, 507
Esp3I CGTCTC 2 cut(s) 442, 653
FaeI CATG 5 cut(s) 16, 269, 298, 464, 626
FaqI GGGAC 2 cut(s) 83, 172
FatI CATG 5 cut(s) 12, 265, 294, 460, 622
Fnu4HI GCNGC 5 cut(s) 81, 84, 122, 449, 693
FokI GGATG 2 cut(s) 489, 560
FriOI GRGCYC 1 cut(s) 725
Fsp4HI GCNGC 5 cut(s) 81, 84, 122, 449, 693
GluI GCNGC 5 cut(s) 81, 84, 122, 449, 693
GsuI CTGGAG 1 cut(s) 27
HapII CCGG 3 cut(s) 146, 210, 707
HgaI GACGC 2 cut(s) 61, 80
Hin1II CATG 5 cut(s) 16, 269, 298, 464, 626
HindIII AAGCTT 1 cut(s) 585
HinfI GANTC 3 cut(s) 47, 410, 599
HpaII CCGG 3 cut(s) 146, 210, 707
HphI GGTGA 1 cut(s) 43
Hpy188I TCNGA 3 cut(s) 46, 185, 303
Hpy188III TCNNGA 4 cut(s) 26, 385, 407, 608
Hpy99I CGWCG 2 cut(s) 55, 579
HpyAV CCTTC 2 cut(s) 298, 352
HpyCH4III ACNGT 2 cut(s) 239, 649
HpyCH4IV ACGT 3 cut(s) 435, 562, 574
HpyCH4V TGCA 5 cut(s) 265, 331, 451, 680, 692
HpyF10VI GCNNNNNNNGC 4 cut(s) 80, 337, 448, 457
HpyF3I CTNAG 1 cut(s) 483
HpySE526I ACGT 3 cut(s) 435, 562, 574
Hsp92II CATG 5 cut(s) 16, 269, 298, 464, 626
KpnI GGTACC 1 cut(s) 707
Kzo9I GATC 3 cut(s) 22, 185, 376
Lsp1109I GCAGC 5 cut(s) 67, 70, 108, 435, 704
LweI GCATC 4 cut(s) 196, 267, 318, 511
MaeII ACGT 3 cut(s) 435, 562, 574
MaeIII GTNAC 2 cut(s) 643, 667
MalI GATC 3 cut(s) 24, 187, 378
MboI GATC 3 cut(s) 22, 185, 376
MboII GAAGA 2 cut(s) 440, 623
MhlI GDGCHC 1 cut(s) 725
MluCI AATT 1 cut(s) 40
MlyI GAGTC 1 cut(s) 593
MmeI TCCRAC 1 cut(s) 633
MnlI CCTC 7 cut(s) 22, 301, 334, 342, 415, 649, 694
MseI TTAA 1 cut(s) 748
MspA1I CMGCKG 1 cut(s) 695
MspI CCGG 3 cut(s) 146, 210, 707
MspR9I CCNGG 3 cut(s) 92, 146, 147
MvaI CCWGG 1 cut(s) 92
MvnI CGCG 1 cut(s) 74
MwoI GCNNNNNNNGC 4 cut(s) 80, 337, 448, 457
NciI CCSGG 2 cut(s) 146, 147
NdeII GATC 3 cut(s) 22, 185, 376
NlaIII CATG 5 cut(s) 16, 269, 298, 464, 626
NlaIV GGNNCC 5 cut(s) 129, 160, 273, 339, 705
NmuCI GTSAC 2 cut(s) 643, 667
PfeI GAWTC 2 cut(s) 47, 410
PflMI CCANNNNNTGG 1 cut(s) 174
PfoI TCCNGGA 1 cut(s) 90
PinAI ACCGGT 1 cut(s) 706
PkrI GCNGC 5 cut(s) 82, 85, 123, 450, 694
PleI GAGTC 1 cut(s) 593
PpsI GAGTC 1 cut(s) 593
Ppu21I YACGTR 1 cut(s) 563
PpuMI RGGWCCY 1 cut(s) 272
Psp124BI GAGCTC 1 cut(s) 725
Psp5II RGGWCCY 1 cut(s) 272
Psp6I CCWGG 1 cut(s) 90
PspGI CCWGG 1 cut(s) 90
PspN4I GGNNCC 5 cut(s) 129, 160, 273, 339, 705
PspPI GGNCC 2 cut(s) 159, 272
PspPPI RGGWCCY 1 cut(s) 272
PvuII CAGCTG 1 cut(s) 695
RsaI GTAC 2 cut(s) 395, 705
RsaNI GTAC 2 cut(s) 394, 704
SacI GAGCTC 1 cut(s) 725
SaqAI TTAA 1 cut(s) 748
SatI GCNGC 5 cut(s) 81, 84, 122, 449, 693
Sau3AI GATC 3 cut(s) 22, 185, 376
Sau96I GGNCC 2 cut(s) 159, 272
ScaI AGTACT 1 cut(s) 395
SchI GAGTC 1 cut(s) 593
ScrFI CCNGG 3 cut(s) 92, 146, 147
SduI GDGCHC 1 cut(s) 725
SfaNI GCATC 4 cut(s) 196, 267, 318, 511
SinI GGWCC 2 cut(s) 159, 272
SmaI CCCGGG 1 cut(s) 147
SmlI CTYRAG 1 cut(s) 419
SmoI CTYRAG 1 cut(s) 419
SnaBI TACGTA 1 cut(s) 563
Sse9I AATT 1 cut(s) 40
SsiI CCGC 1 cut(s) 245
SstI GAGCTC 1 cut(s) 725
StyD4I CCNGG 3 cut(s) 90, 144, 145
StyI CCWWGG 3 cut(s) 63, 275, 507
TaaI ACNGT 2 cut(s) 239, 649
TaiI ACGT 3 cut(s) 438, 565, 577
TaqI TCGA 4 cut(s) 50, 234, 326, 413
TasI AATT 1 cut(s) 40
TatI WGTACW 1 cut(s) 393
TfiI GAWTC 2 cut(s) 47, 410
Tru1I TTAA 1 cut(s) 748
Tru9I TTAA 1 cut(s) 748
TscAI CASTG 3 cut(s) 244, 348, 648
TseFI GTSAC 2 cut(s) 643, 667
TseI GCWGC 5 cut(s) 80, 83, 121, 448, 692
Tsp45I GTSAC 2 cut(s) 643, 667
TspDTI ATGAA 1 cut(s) 29
TspMI CCCGGG 1 cut(s) 145
TspRI CASTG 3 cut(s) 244, 348, 648
Van91I CCANNNNNTGG 1 cut(s) 174
VpaK11BI GGWCC 2 cut(s) 159, 272
XcmI CCANNNNNNNNNTGG 1 cut(s) 629
XmaI CCCGGG 1 cut(s) 145
ZrmI AGTACT 1 cut(s) 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.