Rmu_sc0003594.1_g000022

Carotenoid 9,10(9',10')-cleavage dioxygenase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003594.1
OG0002862
Physical Location & Seq
Forward (+)
132805 .. 134828
2024 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1161 bp
atgagcaacaccaatgttttcgagcattcagggagaatttactcaattgcagagaactatctgcctcaagaggcggacatttcaaccttggaaaccatttgtgattgggatgtcaatggaacttgggatcgacctttcacaagtcatccaaagaaagctccaaacggagagttagtcgtgatggggacagatgcatggaaaccttactatgttttaggggtcatctctgctgatggaaagaaagtgcacaaggttgatctcaaatttaaaagatgtgtgttcagtcatgatataggagtcacgcaaaactacaatgtaatccttgatcatcccctcacattggatgtaaagagactcaccatgggaggccaaataatgcagtatgaaaaggaagagtacgctagaattggggtcatgcctcgttatggagatgcagagtctgtcaaatggtttgaggtacagaccaattgcacacttcactttctcaattgctttgaggatggtaatcaggttatagtcaggggatgtagggctctcacatctgttgtatcagatcctgacggcagcctaaacccttatgaatggttctccaaagggttcatctttgctggtgatcatgattctgcagaggttggatatctctttacgcatttatacgaatggagattgaacatgttatctgggaaagttgaggagagaaacttaactggatccaaatactccatggatttccctttcatcaatgaacaatttattggtttaaaacgcaagtatggatatactcaggtcattgattccggggcaagctctatatgtggcatggctaaatttggatctctagccaaattatatttggaagaatcagattctacaaaatctgtggagggaaggggtgagcacttgataaaggtggaataccataagtttgaggacaacaacttttgcagtggaagtgcttttgtccccagaaatggaggcatcgaggaagatgatggctggattatcacttttgtccacaatgaagaaactgatgtgatccaagttcatatcattgatgcacaggattttgaaggtgaagcgattgcgaaacttacattgccacaacgggtgccctatggttttcattcaacctttctgtcaatgccagcttgtcaatcttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RPE65

Protein Analysis

386

Amino Acids

43.69

Weight (kDa)

5.15

Isoelectric Point (pI)

26.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPE65 PF03055 2 - 379 7.5e-85 Retinal pigment epithelial membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1108
AciI CCGC 1 cut(s) 74
AclWI GGATC 6 cut(s) 135, 548, 705, 718, 841, 1030
AcsI RAATTY 3 cut(s) 36, 263, 827
AfaI GTAC 2 cut(s) 398, 459
AfiI CCNNNNNNNGG 3 cut(s) 340, 425, 971
AflIII ACRYGT 1 cut(s) 672
AgsI TTSAA 4 cut(s) 84, 670, 1070, 1128
AjuI GAANNNNNNNTTGG 2 cut(s) 738, 770
AluBI AGCT 3 cut(s) 158, 807, 1148
AluI AGCT 3 cut(s) 158, 807, 1148
Alw21I GWGCWC 2 cut(s) 249, 900
Alw26I GTCTC 1 cut(s) 346
Alw44I GTGCAC 1 cut(s) 245
AlwI GGATC 6 cut(s) 135, 548, 705, 718, 841, 1030
AlwNI CAGNNNCTG 2 cut(s) 440, 557
AoxI GGCC 1 cut(s) 367
ApaLI GTGCAC 1 cut(s) 245
ApeKI GCWGC 1 cut(s) 564
ApoI RAATTY 3 cut(s) 36, 263, 827
AsuC2I CCSGG 1 cut(s) 799
AsuHPI GGTGA 4 cut(s) 349, 623, 905, 1085
BaeGI GKGCMC 2 cut(s) 249, 1113
BamHI GGATCC 1 cut(s) 710
BanI GGYRCC 1 cut(s) 1108
BanII GRGCYC 1 cut(s) 535
Bbv12I GWGCWC 2 cut(s) 249, 900
BbvI GCAGC 1 cut(s) 576
BccI CCATC 4 cut(s) 175, 227, 494, 986
BceAI ACGGC 1 cut(s) 577
BclI TGATCA 2 cut(s) 325, 613
BcnI CCSGG 1 cut(s) 799
BcoDI GTCTC 1 cut(s) 346
BfaI CTAG 2 cut(s) 402, 839
BfmI CTRYAG 1 cut(s) 624
BisI GCNGC 1 cut(s) 565
BlsI GCNGC 1 cut(s) 566
Bme1390I CCNGG 1 cut(s) 799
BmiI GGNNCC 2 cut(s) 712, 1110
BmrFI CCNGG 1 cut(s) 799
BmsI GCATC 4 cut(s) 181, 421, 987, 1045
BpuEI CTTGAG 1 cut(s) 51
BpuMI CCSGG 1 cut(s) 799
BsaBI GATNNNNATC 1 cut(s) 504
BsaJI CCNNGG 4 cut(s) 87, 360, 723, 798
BsaXI ACNNNNNCTCC 2 cut(s) 159, 189
Bsc4I CCNNNNNNNGG 3 cut(s) 340, 425, 971
Bse1I ACTGG 1 cut(s) 712
Bse3DI GCAATG 1 cut(s) 1094
Bse8I GATNNNNATC 1 cut(s) 504
BseDI CCNNGG 4 cut(s) 87, 360, 723, 798
BseGI GGATG 6 cut(s) 115, 145, 328, 349, 505, 530
BseJI GATNNNNATC 1 cut(s) 504
BseLI CCNNNNNNNGG 3 cut(s) 340, 425, 971
BseMI GCAATG 1 cut(s) 1094
BseMII CTCAG 1 cut(s) 797
BseNI ACTGG 1 cut(s) 712
BseRI GAGGAG 1 cut(s) 707
BseSI GKGCMC 2 cut(s) 249, 1113
BseXI GCAGC 1 cut(s) 576
BshFI GGCC 1 cut(s) 369
BshNI GGYRCC 1 cut(s) 1108
BsiHKAI GWGCWC 2 cut(s) 249, 900
BsiSI CCGG 1 cut(s) 798
BslFI GGGAC 2 cut(s) 199, 947
BslI CCNNNNNNNGG 3 cut(s) 340, 425, 971
BsmAI GTCTC 1 cut(s) 346
BsmFI GGGAC 2 cut(s) 199, 947
BsmI GAATGC 1 cut(s) 25
BsnI GGCC 1 cut(s) 369
Bsp1286I GDGCHC 4 cut(s) 249, 535, 900, 1113
Bsp143I GATC 8 cut(s) 127, 256, 325, 553, 613, 710, 833, 1035
Bsp19I CCATGG 2 cut(s) 360, 723
BspACI CCGC 1 cut(s) 74
BspANI GGCC 1 cut(s) 369
BspCNI CTCAG 1 cut(s) 796
BspHI TCATGA 2 cut(s) 286, 616
BspLI GGNNCC 2 cut(s) 712, 1110
BspMAI CTGCAG 1 cut(s) 628
BspPI GGATC 6 cut(s) 135, 548, 705, 718, 841, 1030
BspT107I GGYRCC 1 cut(s) 1108
BsrDI GCAATG 1 cut(s) 1094
BsrI ACTGG 1 cut(s) 712
BssECI CCNNGG 4 cut(s) 87, 360, 723, 798
BssMI GATC 8 cut(s) 127, 256, 325, 553, 613, 710, 833, 1035
BssT1I CCWWGG 3 cut(s) 87, 360, 723
Bst6I CTCTTC 1 cut(s) 387
BstC8I GCNNGC 2 cut(s) 805, 1146
BstDEI CTNAG 2 cut(s) 783, 1158
BstDSI CCRYGG 2 cut(s) 360, 723
BstF5I GGATG 6 cut(s) 115, 145, 328, 349, 505, 530
BstKTI GATC 8 cut(s) 130, 259, 328, 556, 616, 713, 836, 1038
BstMAI GTCTC 1 cut(s) 346
BstMBI GATC 8 cut(s) 127, 256, 325, 553, 613, 710, 833, 1035
BstNSI RCATGY 1 cut(s) 676
BstSCI CCNGG 1 cut(s) 797
BstSFI CTRYAG 1 cut(s) 624
BstSLI GKGCMC 2 cut(s) 249, 1113
BstV1I GCAGC 1 cut(s) 576
BstX2I RGATCY 3 cut(s) 553, 710, 833
BstYI RGATCY 3 cut(s) 553, 710, 833
BsuRI GGCC 1 cut(s) 369
BtgI CCRYGG 2 cut(s) 360, 723
BtsCI GGATG 6 cut(s) 115, 145, 328, 349, 505, 530
BtsI GCAGTG 1 cut(s) 952
BtsIMutI CAGTG 1 cut(s) 952
Cac8I GCNNGC 2 cut(s) 805, 1146
CaiI CAGNNNCTG 2 cut(s) 440, 557
CciI TCATGA 2 cut(s) 286, 616
Csp6I GTAC 2 cut(s) 397, 458
CspCI CAANNNNNGTGG 2 cut(s) 861, 896
CviAII CATG 8 cut(s) 195, 287, 361, 415, 617, 673, 724, 820
CviJI RGCY 9 cut(s) 158, 369, 533, 567, 807, 824, 842, 996, 1148
CviKI_1 RGCY 9 cut(s) 158, 369, 533, 567, 807, 824, 842, 996, 1148
CviQI GTAC 2 cut(s) 397, 458
DdeI CTNAG 2 cut(s) 783, 1158
DpnI GATC 8 cut(s) 129, 258, 327, 555, 615, 712, 835, 1037
DpnII GATC 8 cut(s) 127, 256, 325, 553, 613, 710, 833, 1035
DraI TTTAAA 2 cut(s) 268, 762
Eam1104I CTCTTC 1 cut(s) 387
EarI CTCTTC 1 cut(s) 387
EciI GGCGGA 1 cut(s) 89
Eco130I CCWWGG 3 cut(s) 87, 360, 723
Eco24I GRGCYC 1 cut(s) 535
Eco32I GATATC 1 cut(s) 638
EcoRV GATATC 1 cut(s) 638
EcoT14I CCWWGG 3 cut(s) 87, 360, 723
EcoT22I ATGCAT 1 cut(s) 196
EcoT38I GRGCYC 1 cut(s) 535
ErhI CCWWGG 3 cut(s) 87, 360, 723
FaeI CATG 8 cut(s) 198, 290, 364, 418, 620, 676, 727, 823
FaqI GGGAC 2 cut(s) 199, 947
FatI CATG 8 cut(s) 194, 286, 360, 414, 616, 672, 723, 819
FbaI TGATCA 2 cut(s) 325, 613
Fnu4HI GCNGC 1 cut(s) 565
FokI GGATG 6 cut(s) 122, 132, 315, 356, 512, 537
FriOI GRGCYC 1 cut(s) 535
Fsp4HI GCNGC 1 cut(s) 565
FspBI CTAG 2 cut(s) 402, 839
GluI GCNGC 1 cut(s) 565
HaeIII GGCC 1 cut(s) 369
HapII CCGG 1 cut(s) 798
Hin1II CATG 8 cut(s) 198, 290, 364, 418, 620, 676, 727, 823
HinfI GANTC 7 cut(s) 297, 354, 437, 620, 794, 860, 866
HpaII CCGG 1 cut(s) 798
HphI GGTGA 4 cut(s) 349, 623, 905, 1085
Hpy166II GTNNAC 2 cut(s) 247, 1015
Hpy188I TCNGA 2 cut(s) 553, 865
Hpy188III TCNNGA 5 cut(s) 68, 178, 287, 557, 617
Hpy8I GTNNAC 2 cut(s) 247, 1015
HpyAV CCTTC 2 cut(s) 882, 1064
HpyCH4V TGCA 9 cut(s) 50, 194, 247, 379, 434, 471, 626, 945, 1058
HpyF3I CTNAG 2 cut(s) 783, 1158
Hsp92II CATG 8 cut(s) 198, 290, 364, 418, 620, 676, 727, 823
Ksp22I TGATCA 2 cut(s) 325, 613
Kzo9I GATC 8 cut(s) 127, 256, 325, 553, 613, 710, 833, 1035
LmnI GCTCC 1 cut(s) 163
Lsp1109I GCAGC 1 cut(s) 576
LweI GCATC 4 cut(s) 181, 421, 987, 1045
MaeI CTAG 2 cut(s) 402, 839
MaeIII GTNAC 1 cut(s) 298
MalI GATC 8 cut(s) 129, 258, 327, 555, 615, 712, 835, 1037
MboI GATC 8 cut(s) 127, 256, 325, 553, 613, 710, 833, 1035
MboII GAAGA 4 cut(s) 404, 869, 998, 1034
MfeI CAATTG 3 cut(s) 45, 466, 487
MflI RGATCY 3 cut(s) 553, 710, 833
MhlI GDGCHC 4 cut(s) 249, 535, 900, 1113
MluCI AATT 9 cut(s) 36, 45, 263, 405, 466, 487, 749, 827, 845
MlyI GAGTC 3 cut(s) 306, 348, 446
MmeI TCCRAC 1 cut(s) 613
Mph1103I ATGCAT 1 cut(s) 196
MseI TTAA 3 cut(s) 267, 704, 761
MspI CCGG 1 cut(s) 798
MspR9I CCNGG 1 cut(s) 799
MunI CAATTG 3 cut(s) 45, 466, 487
Mva1269I GAATGC 1 cut(s) 25
NciI CCSGG 1 cut(s) 799
NcoI CCATGG 2 cut(s) 360, 723
NdeII GATC 8 cut(s) 127, 256, 325, 553, 613, 710, 833, 1035
NlaIII CATG 8 cut(s) 198, 290, 364, 418, 620, 676, 727, 823
NlaIV GGNNCC 2 cut(s) 712, 1110
NmuCI GTSAC 1 cut(s) 298
NsiI ATGCAT 1 cut(s) 196
NspI RCATGY 1 cut(s) 676
PagI TCATGA 2 cut(s) 286, 616
PciI ACATGT 1 cut(s) 672
PctI GAATGC 1 cut(s) 25
PfeI GAWTC 4 cut(s) 620, 794, 860, 866
PkrI GCNGC 1 cut(s) 566
PleI GAGTC 3 cut(s) 305, 348, 445
PpsI GAGTC 3 cut(s) 305, 348, 445
PscI ACATGT 1 cut(s) 672
PspN4I GGNNCC 2 cut(s) 712, 1110
PstI CTGCAG 1 cut(s) 628
PstNI CAGNNNCTG 2 cut(s) 440, 557
PsuI RGATCY 3 cut(s) 553, 710, 833
RsaI GTAC 2 cut(s) 398, 459
RsaNI GTAC 2 cut(s) 397, 458
SaqAI TTAA 3 cut(s) 267, 704, 761
SatI GCNGC 1 cut(s) 565
Sau3AI GATC 8 cut(s) 127, 256, 325, 553, 613, 710, 833, 1035
SchI GAGTC 3 cut(s) 306, 348, 446
ScrFI CCNGG 1 cut(s) 799
SduI GDGCHC 4 cut(s) 249, 535, 900, 1113
SfaNI GCATC 4 cut(s) 181, 421, 987, 1045
SfcI CTRYAG 1 cut(s) 624
SmlI CTYRAG 1 cut(s) 66
SmoI CTYRAG 1 cut(s) 66
Sse9I AATT 9 cut(s) 36, 45, 263, 405, 466, 487, 749, 827, 845
SsiI CCGC 1 cut(s) 74
SspMI CTAG 2 cut(s) 402, 839
StyD4I CCNGG 1 cut(s) 797
StyI CCWWGG 3 cut(s) 87, 360, 723
TaqI TCGA 3 cut(s) 21, 130, 981
TasI AATT 9 cut(s) 36, 45, 263, 405, 466, 487, 749, 827, 845
TfiI GAWTC 4 cut(s) 620, 794, 860, 866
Tru1I TTAA 3 cut(s) 267, 704, 761
Tru9I TTAA 3 cut(s) 267, 704, 761
TscAI CASTG 1 cut(s) 952
TseFI GTSAC 1 cut(s) 298
TseI GCWGC 1 cut(s) 564
Tsp45I GTSAC 1 cut(s) 298
TspDTI ATGAA 8 cut(s) 399, 589, 594, 727, 759, 1034, 1035, 1112
TspGWI ACGGA 1 cut(s) 180
TspRI CASTG 1 cut(s) 952
VneI GTGCAC 1 cut(s) 245
XapI RAATTY 3 cut(s) 36, 263, 827
XceI RCATGY 1 cut(s) 676
XcmI CCANNNNNNNNNTGG 2 cut(s) 721, 850
XspI CTAG 2 cut(s) 402, 839
Zsp2I ATGCAT 1 cut(s) 196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.