Rmu_sc0003822.1_g000020

SH3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003822.1
OG0004649
Physical Location & Seq
Reverse (-)
55583 .. 75032
19450 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3612 bp
atggcggactcgtcagggacgaccctgatggatctgataacggcggacccttccacagtatcagcttccacctcctcaacgacgtcgtcttcgtcttcgtcttccgcgcctcctcctcctcctccttatgcggcttcacccccaccgcccagaggcaccagccccggctccgctctcggaaagcctgctgtcgagaagaggtccaagagggctgcgcttatgcagatccagaacgacaccatctctgccgccaaagctgccctcaatcccgttcgcaccaacatcatcatgccccagaagcacaggcacaagcagaaaaagcctgtttcatatgctcaacttgcaaggagcattcacgaactagctgctacctccgatcagaaaagttcccagaagcagttggtgaatcacgtctttcccaagctcgccgtctacaattctgttgacccctctgtcgccccttctcttctcatgctcaatcagcagtgtgaggataagagcgttctgcgatatgtctattattacttggccaggattttatcagatactggtgcacaaggtgtaaccacaggcggtgggatcccaactccaaactgggatgctctagctgacattgatgctattggaggggtaactagagctgatgttgtaccaagaatagtaaatcagctcactatagaagcttcaaatgctgatgcagaatttcatgcacgaagactgcaagctttgaaggctcttacatatgctccatcaacaaattctgaaattttgtccaaattgtatgaaattgtgtttggcattcttgagaaggttgctgatggcccgcaaaaacgcaagaaaggtgtgtttgggactaaaggtggcgataaagagtttatcatccgaagtaacttgcaatatgctgccctaagtgctcttagaagacttccattggatccaggaaacccagcttttttgtaccgtgctgttcaaggggtgtcatttgctgatcctgttgctgtaaggcatgctttggaaattctgtctgagctagccacaaaagacccgtatgcagttgcaatgggcttagggaaacatgcagagcctggaggggctttgcaggatgttctccatttgcatgatgttcttgctagagttgctctagctaggttgtgttatacaatatcaagagcacgagcattggatgacaggcctgatattagatctcagttcaactctgtactctatcaactcctcctggatcccagcgaaagagtctgttttgaggcaatcttatgtatattgggaaaacacgataacacagaaaggactgatgatcgagctgctggatggtatcgattaactagagagattctcaagttgccagaagcaccttctgtgaaggattcctccaaagataaagttcaaaagacaaggcgtccacaaccccttattaaacttagttctttcagaagtttttctaggccagtactccatgcagcatcaagagttgtgcaggagatgggaaagagtcgggctgccgcatttgctctaggcatacaggatatcgacgaatcagtgcatgttaacacattttctgagactcctgattcacgcgagatagatagtagtgaagcctcacatcctgagagtatccgaagaacctcttccttacctactggggtaggtggcaaggatacaatagctagtttgttagcttcattaatggaagttgtgcggacaactgtggcatgcgagtgtgtttatgttcgagcaatggttattaaggcattgatctggatgcaaagtccacatgattcgtttgatcaactagaatctataattgcatcagagctttctgatccggcttggccagccacgctcttgaatgatattttactcactttgcatgctcggtttaaggcaactccagatatggccgtcactcttcttgaaattgcaaggatctttgcaactaaagccccggggaagatcgatgctgatgtactacaactactgtggaagacttgtcttgttggagctggtcctgatgggaaacacactgcattagaagcggttaccatagttctcgatttaccaccaccacaacctgggtcaatgttggggcttacatcagttgacagggtatctgcatctgatccaaaggcagccctggctttacagagattagtacaagcagcagtatggtttcttggagaaaatgcaaactatgctgcttctgaatatgcttgggaatccgcaacccctcctggtaccgcattgatgatgctagatgcagataaaatggttgctgctgccagttcccgcaaccctactctagctggtgcattgactaggctacagaggtgtgcattcagtggcaactgggaggttcggatcattgctgctcaagctctcacaactatggcaataagatctggtgagccttttaggctacaaatctatgaatttttacacactatagcacagggcggagtgcagtctcagttttctgacatgcatcccagtaatggagaagaccaaggagctagtggcacaggccttggagttctaatcagtcccatgatagaggttcttgatgaaatgtatagggcacaagatgatttgatcaaggaaatgcgcaaccatgataatgctaataaggaatggacagatgaagagcttaagaaactctatgaaacccatgaaaggttgcttgatcttgtttctcttttttgttatgttccaagagcaaaatatctgcctttggggccaataagtgcgaaacttatcgacatctatcgcaccaggcataatattagtgcatcaactggtttgagtgatccagctgttgctactggaatttctgatcttatgtatgaatctaaaccagcagctgtagagcctgatgtgcttgatgatgaccttgtaaatgcttgggcagcaaaccttggtgatgacggactgttaggaaataatgcaccagcaatgagcagggttaatgaatttctagctggtgctggaactgatgctcctgatgttgatgaggaaaacatcatctctaggccgtcagttggttacgatgatatgtgggcaaagacccttttagagacttcagaactggaggaagaagacgcgaggtcatccgggacatcttcacctgaatcaacaggctcggtcgaaacttccatatcatcgcattttgggggaatgaattacccttcacttttcagttcacggccggagaggtctggaggaagcagatatagcaatccatctatggggggtccctcattcaatgagggtttaggatctccaattagagaagagcctccgccatactcatcacctgcaatgcaaagatttgagtcgtttgagaatcccttagctgggcatggatcccagagttttggatcccaagatgacgaacgcagttcttctggaaatcctcaacatggaacagcactttatgacttcactgctggtggagatgatgagttaaatttaacagctggagaggaggttgatatcgagtacgaagtggatggctggttttatgtaaagaagaaacgccccgggagggatgggaaaatggctggtctggtccctgtcctctatgttagtcagtcttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SH3_1 SH3_9

Protein Analysis

1203

Amino Acids

130.07

Weight (kDa)

5.44

Isoelectric Point (pI)

48.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SH3_1 PF00018 1147 - 1188 7.1e-06 SH3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3337
AasI GACNNNNNNGTC 1 cut(s) 452
AatII GACGTC 1 cut(s) 86
Acc16I TGCGCA 1 cut(s) 2621
Acc36I ACCTGC 1 cut(s) 3337
Acc65I GGTACC 1 cut(s) 2252
AccB1I GGYRCC 2 cut(s) 155, 2252
AccB7I CCANNNNNTGG 1 cut(s) 2090
AccBSI CCGCTC 1 cut(s) 173
AccI GTMKAC 1 cut(s) 432
AccII CGCG 3 cut(s) 107, 1596, 3116
AcoI YGGCCR 4 cut(s) 528, 1850, 1917, 3218
AcsI RAATTY 8 cut(s) 701, 757, 765, 1017, 2447, 2841, 2984, 3481
AcuI CTGAAG 1 cut(s) 3078
AcyI GRCGYC 2 cut(s) 83, 1417
AdeI CACNNNGTG 1 cut(s) 560
AfaI GTAC 8 cut(s) 651, 959, 1221, 1470, 1986, 2172, 2254, 3515
AflII CTTAAG 1 cut(s) 2663
AgsI TTSAA 8 cut(s) 687, 730, 971, 1213, 1406, 1867, 1934, 3277
AhdI GACNNNNNGTC 2 cut(s) 1416, 3118
AjiI CACGTC 1 cut(s) 412
AjnI CCWGG 8 cut(s) 530, 937, 1084, 1236, 2089, 2151, 2248, 2786
AjuI GAANNNNNNNTTGG 2 cut(s) 777, 809
AloI GAACNNNNNNTCC 2 cut(s) 2995, 3027
Alw21I GWGCWC 3 cut(s) 556, 916, 1174
Alw26I GTCTC 3 cut(s) 1574, 2487, 3083
Alw44I GTGCAC 1 cut(s) 552
AlwNI CAGNNNCTG 3 cut(s) 548, 1085, 2876
Ama87I CYCGRG 2 cut(s) 1963, 3554
ApaLI GTGCAC 1 cut(s) 552
ApoI RAATTY 8 cut(s) 701, 757, 765, 1017, 2447, 2841, 2984, 3481
ArsI GACNNNNNNTTYG 2 cut(s) 73, 105
AseI ATTAAT 1 cut(s) 1703
Asp700I GAANNNNTTC 2 cut(s) 1456, 1645
Asp718I GGTACC 1 cut(s) 2252
AspLEI GCGC 3 cut(s) 109, 217, 2622
AspS9I GGNCC 7 cut(s) 46, 201, 821, 2024, 2750, 3266, 3583
AsuC2I CCSGG 6 cut(s) 165, 1964, 1965, 3127, 3555, 3556
AsuHPI GGTGA 6 cut(s) 129, 415, 2432, 2945, 3129, 3318
AsuNHI GCTAGC 1 cut(s) 1030
AvaI CYCGRG 2 cut(s) 1963, 3554
AvaII GGWCC 5 cut(s) 46, 201, 2024, 3266, 3583
BaeGI GKGCMC 2 cut(s) 556, 2596
BalI TGGCCA 2 cut(s) 530, 1852
BamHI GGATCC 5 cut(s) 579, 934, 1240, 3377, 3392
BanI GGYRCC 2 cut(s) 155, 2252
BauI CACGAG 1 cut(s) 1173
BbsI GAAGAC 8 cut(s) 81, 87, 93, 721, 928, 2009, 2523, 3117
Bbv12I GWGCWC 3 cut(s) 556, 916, 1174
BceAI ACGGC 5 cut(s) 57, 413, 1904, 3031, 3233
BcgI CGANNNNNNTGC 2 cut(s) 347, 381
BciT130I CCWGG 8 cut(s) 532, 939, 1086, 1238, 2091, 2153, 2250, 2788
BciVI GTATCC 2 cut(s) 1643, 1669
BclI TGATCA 2 cut(s) 1804, 2607
BcnI CCSGG 6 cut(s) 165, 1964, 1965, 3127, 3555, 3556
BcoDI GTCTC 3 cut(s) 1574, 2487, 3083
BfmI CTRYAG 4 cut(s) 675, 2339, 2460, 2877
BfrI CTTAAG 1 cut(s) 2663
BfuAI ACCTGC 1 cut(s) 3337
BfuI GTATCC 2 cut(s) 1643, 1669
BglI GCCNNNNNGGC 2 cut(s) 2431, 2749
BglII AGATCT 2 cut(s) 1202, 2414
BmcAI AGTACT 1 cut(s) 1470
Bme18I GGWCC 5 cut(s) 46, 201, 2024, 3266, 3583
BmeRI GACNNNNNGTC 2 cut(s) 1416, 3118
BmeT110I CYCGRG 2 cut(s) 1963, 3554
BmgBI CACGTC 1 cut(s) 412
BmgT120I GGNCC 7 cut(s) 46, 201, 821, 2024, 2750, 3266, 3583
BmrI ACTGGG 4 cut(s) 604, 1668, 2374, 2499
BmtI GCTAGC 1 cut(s) 1034
BmuI ACTGGG 4 cut(s) 604, 1668, 2374, 2499
BpiI GAAGAC 8 cut(s) 81, 87, 93, 721, 928, 2009, 2523, 3117
BplI GAGNNNNNCTC 2 cut(s) 1338, 1370
BpmI CTGGAG 5 cut(s) 1107, 1893, 3122, 3252, 3513
Bpu10I CCTNAGC 2 cut(s) 1066, 3364
BpuEI CTTGAG 3 cut(s) 824, 1340, 2373
BpuMI CCSGG 6 cut(s) 165, 1964, 1965, 3127, 3555, 3556
Bsa29I ATCGAT 2 cut(s) 1336, 1974
BsaHI GRCGYC 2 cut(s) 83, 1417
BsaXI ACNNNNNCTCC 6 cut(s) 730, 760, 2995, 3025, 3486, 3516
Bse3DI GCAATG 5 cut(s) 1065, 1761, 2379, 2973, 3339
BseBI CCWGG 8 cut(s) 532, 939, 1086, 1238, 2091, 2153, 2250, 2788
BseCI ATCGAT 2 cut(s) 1336, 1974
BseMI GCAATG 5 cut(s) 1065, 1761, 2379, 2973, 3339
BseMII CTCAG 5 cut(s) 1017, 1220, 1569, 1617, 2498
BseRI GAGGAG 7 cut(s) 64, 102, 105, 108, 111, 1223, 3512
BseSI GKGCMC 2 cut(s) 556, 2596
BseX3I CGGCCG 1 cut(s) 3218
BseYI CCCAGC 3 cut(s) 946, 1244, 3368
BsgI GTGCAG 2 cut(s) 1514, 2498
Bsh1236I CGCG 3 cut(s) 107, 1596, 3116
Bsh1285I CGRYCG 2 cut(s) 3159, 3221
BshNI GGYRCC 2 cut(s) 155, 2252
BshVI ATCGAT 2 cut(s) 1336, 1974
BsiEI CGRYCG 2 cut(s) 3159, 3221
BsiHKAI GWGCWC 3 cut(s) 556, 916, 1174
BsiHKCI CYCGRG 2 cut(s) 1963, 3554
BsiSI CCGG 6 cut(s) 165, 1844, 1964, 3126, 3221, 3555
BslFI GGGAC 6 cut(s) 31, 865, 2544, 3142, 3252, 3569
BsmAI GTCTC 3 cut(s) 1574, 2487, 3083
BsmFI GGGAC 6 cut(s) 31, 865, 2544, 3142, 3252, 3569
BsmI GAATGC 3 cut(s) 351, 798, 2351
BsoBI CYCGRG 2 cut(s) 1963, 3554
Bsp1286I GDGCHC 4 cut(s) 556, 916, 1174, 2596
BspCNI CTCAG 5 cut(s) 1018, 1219, 1570, 1618, 2497
BspDI ATCGAT 2 cut(s) 1336, 1974
BspFNI CGCG 3 cut(s) 107, 1596, 3116
BspMI ACCTGC 1 cut(s) 3337
BspOI GCTAGC 1 cut(s) 1034
BspQI GCTCTTC 2 cut(s) 2652, 3300
BspT107I GGYRCC 2 cut(s) 155, 2252
BspTI CTTAAG 1 cut(s) 2663
BsrBI CCGCTC 1 cut(s) 173
BsrDI GCAATG 5 cut(s) 1065, 1761, 2379, 2973, 3339
BssNI GRCGYC 2 cut(s) 83, 1417
BssSI CACGAG 1 cut(s) 1173
BssT1I CCWWGG 3 cut(s) 2521, 2542, 2929
Bst2BI CACGAG 1 cut(s) 1173
Bst2UI CCWGG 8 cut(s) 532, 939, 1086, 1238, 2091, 2153, 2250, 2788
Bst4CI ACNGT 5 cut(s) 58, 962, 1726, 1998, 2946
Bst6I CTCTTC 6 cut(s) 191, 471, 1651, 1932, 2652, 3300
BstACI GRCGYC 2 cut(s) 83, 1417
BstAFI CTTAAG 1 cut(s) 2663
BstAPI GCANNNNNTGC 1 cut(s) 2210
BstC8I GCNNGC 9 cut(s) 186, 426, 723, 824, 1008, 1032, 1732, 1854, 1890
BstEII GGTNACC 1 cut(s) 2056
BstFNI CGCG 3 cut(s) 107, 1596, 3116
BstHHI GCGC 3 cut(s) 109, 217, 2622
BstMAI GTCTC 3 cut(s) 1574, 2487, 3083
BstMCI CGRYCG 2 cut(s) 3159, 3221
BstNI CCWGG 8 cut(s) 532, 939, 1086, 1238, 2091, 2153, 2250, 2788
BstNSI RCATGY 6 cut(s) 1010, 1079, 1565, 1734, 1892, 2500
BstPI GGTNACC 1 cut(s) 2056
BstSFI CTRYAG 4 cut(s) 675, 2339, 2460, 2877
BstSLI GKGCMC 2 cut(s) 556, 2596
BstUI CGCG 3 cut(s) 107, 1596, 3116
BstV2I GAAGAC 8 cut(s) 81, 87, 93, 721, 928, 2009, 2523, 3117
BstXI CCANNNNNNTGG 1 cut(s) 3389
BstZI CGGCCG 1 cut(s) 3218
Bsu15I ATCGAT 2 cut(s) 1336, 1974
BsuI GTATCC 2 cut(s) 1643, 1669
BsuTUI ATCGAT 2 cut(s) 1336, 1974
BtgZI GCGATG 1 cut(s) 3159
BtrI CACGTC 1 cut(s) 412
BtsI GCAGTG 3 cut(s) 491, 2040, 3456
BtsIMutI CAGTG 5 cut(s) 491, 1563, 2040, 2362, 3456
BveI ACCTGC 1 cut(s) 3337
Cac8I GCNNGC 9 cut(s) 186, 426, 723, 824, 1008, 1032, 1732, 1854, 1890
CaiI CAGNNNCTG 3 cut(s) 548, 1085, 2876
CfoI GCGC 3 cut(s) 109, 217, 2622
Cfr13I GGNCC 7 cut(s) 46, 201, 821, 2024, 2750, 3266, 3583
Cfr9I CCCGGG 2 cut(s) 1963, 3554
ClaI ATCGAT 2 cut(s) 1336, 1974
CseI GACGC 2 cut(s) 1406, 3122
Csp6I GTAC 8 cut(s) 650, 958, 1220, 1469, 1985, 2171, 2253, 3514
CspCI CAANNNNNGTGG 2 cut(s) 1979, 2014
CviQI GTAC 8 cut(s) 650, 958, 1220, 1469, 1985, 2171, 2253, 3514
DraIII CACNNNGTG 1 cut(s) 560
DrdI GACNNNNNNGTC 1 cut(s) 452
DriI GACNNNNNGTC 2 cut(s) 1416, 3118
DseDI GACNNNNNNGTC 1 cut(s) 452
EaeI YGGCCR 4 cut(s) 528, 1850, 1917, 3218
EagI CGGCCG 1 cut(s) 3218
Eam1104I CTCTTC 6 cut(s) 191, 471, 1651, 1932, 2652, 3300
Eam1105I GACNNNNNGTC 2 cut(s) 1416, 3118
EarI CTCTTC 6 cut(s) 191, 471, 1651, 1932, 2652, 3300
EciI GGCGGA 4 cut(s) 20, 59, 2487, 3303
EclXI CGGCCG 1 cut(s) 3218
Eco130I CCWWGG 3 cut(s) 2521, 2542, 2929
Eco147I AGGCCT 2 cut(s) 1192, 2541
Eco32I GATATC 2 cut(s) 1546, 3508
Eco47I GGWCC 5 cut(s) 46, 201, 2024, 3266, 3583
Eco52I CGGCCG 1 cut(s) 3218
Eco57I CTGAAG 1 cut(s) 3078
Eco88I CYCGRG 2 cut(s) 1963, 3554
Eco91I GGTNACC 1 cut(s) 2056
EcoO109I RGGNCCY 1 cut(s) 3266
EcoO65I GGTNACC 1 cut(s) 2056
EcoRII CCWGG 8 cut(s) 530, 937, 1084, 1236, 2089, 2151, 2248, 2786
EcoRV GATATC 2 cut(s) 1546, 3508
EcoT14I CCWWGG 3 cut(s) 2521, 2542, 2929
EcoT22I ATGCAT 1 cut(s) 2502
ErhI CCWWGG 3 cut(s) 2521, 2542, 2929
FalI AAGNNNNNCTT 4 cut(s) 994, 1026, 1630, 1662
FaqI GGGAC 6 cut(s) 31, 865, 2544, 3142, 3252, 3569
FauI CCCGC 2 cut(s) 831, 2312
FauNDI CATATG 2 cut(s) 331, 742
FbaI TGATCA 2 cut(s) 1804, 2607
FblI GTMKAC 1 cut(s) 432
FspI TGCGCA 1 cut(s) 2621
GlaI GCGC 3 cut(s) 108, 216, 2621
GsaI CCCAGC 3 cut(s) 950, 1248, 3372
GsuI CTGGAG 5 cut(s) 1107, 1893, 3122, 3252, 3513
HapII CCGG 6 cut(s) 165, 1844, 1964, 3126, 3221, 3555
HgaI GACGC 2 cut(s) 1406, 3122
HhaI GCGC 3 cut(s) 109, 217, 2622
Hin1I GRCGYC 2 cut(s) 83, 1417
Hin6I GCGC 3 cut(s) 107, 215, 2620
HinP1I GCGC 3 cut(s) 107, 215, 2620
HincII GTYRAC 3 cut(s) 445, 1567, 2119
HindII GTYRAC 3 cut(s) 445, 1567, 2119
HindIII AAGCTT 2 cut(s) 681, 723
HpaI GTTAAC 1 cut(s) 1567
HpaII CCGG 6 cut(s) 165, 1844, 1964, 3126, 3221, 3555
HphI GGTGA 6 cut(s) 129, 415, 2432, 2945, 3129, 3318
Hpy166II GTNNAC 8 cut(s) 433, 445, 554, 1421, 1567, 1790, 2119, 3215
Hpy8I GTNNAC 8 cut(s) 433, 445, 554, 1421, 1567, 1790, 2119, 3215
Hpy99I CGWCG 3 cut(s) 85, 88, 1553
HpyAV CCTTC 7 cut(s) 60, 471, 724, 802, 1375, 1383, 3210
HpyCH4III ACNGT 5 cut(s) 58, 962, 1726, 1998, 2946
HpyCH4IV ACGT 2 cut(s) 83, 411
HpySE526I ACGT 2 cut(s) 83, 411
Hsp92I GRCGYC 2 cut(s) 83, 1417
HspAI GCGC 3 cut(s) 107, 215, 2620
KflI GGGWCCC 1 cut(s) 3266
KpnI GGTACC 1 cut(s) 2256
Ksp22I TGATCA 2 cut(s) 1804, 2607
KspAI GTTAAC 1 cut(s) 1567
LguI GCTCTTC 2 cut(s) 2652, 3300
LmnI GCTCC 6 cut(s) 173, 348, 751, 2018, 2525, 3016
MaeII ACGT 2 cut(s) 83, 411
MaeIII GTNAC 6 cut(s) 562, 631, 887, 1921, 2056, 3056
MbiI CCGCTC 1 cut(s) 173
MhlI GDGCHC 4 cut(s) 556, 916, 1174, 2596
MlsI TGGCCA 2 cut(s) 530, 1852
MluNI TGGCCA 2 cut(s) 530, 1852
MlyI GAGTC 5 cut(s) 2, 1263, 1519, 1576, 3356
MmeI TCCRAC 1 cut(s) 1996
Mox20I TGGCCA 2 cut(s) 530, 1852
Mph1103I ATGCAT 1 cut(s) 2502
MroXI GAANNNNTTC 2 cut(s) 1456, 1645
MscI TGGCCA 2 cut(s) 530, 1852
MslI CAYNNNNRTG 5 cut(s) 287, 1116, 1735, 2402, 2631
Msp20I TGGCCA 2 cut(s) 530, 1852
MspA1I CMGCKG 3 cut(s) 2828, 2876, 3491
MspCI CTTAAG 1 cut(s) 2663
MspI CCGG 6 cut(s) 165, 1844, 1964, 3126, 3221, 3555
Mva1269I GAATGC 3 cut(s) 351, 798, 2351
MvaI CCWGG 8 cut(s) 532, 939, 1086, 1238, 2091, 2153, 2250, 2788
MvnI CGCG 3 cut(s) 107, 1596, 3116
NciI CCSGG 6 cut(s) 165, 1964, 1965, 3127, 3555, 3556
NdeI CATATG 2 cut(s) 331, 742
NheI GCTAGC 1 cut(s) 1030
NmuCI GTSAC 1 cut(s) 1921
NsbI TGCGCA 1 cut(s) 2621
NsiI ATGCAT 1 cut(s) 2502
NspI RCATGY 6 cut(s) 1010, 1079, 1565, 1734, 1892, 2500
PaeI GCATGC 3 cut(s) 1010, 1734, 1892
PaqCI CACCTGC 1 cut(s) 3337
PceI AGGCCT 2 cut(s) 1192, 2541
PciSI GCTCTTC 2 cut(s) 2652, 3300
PcsI WCGNNNNNNNCGW 2 cut(s) 17, 89
PctI GAATGC 3 cut(s) 351, 798, 2351
PdmI GAANNNNTTC 2 cut(s) 1456, 1645
PflFI GACNNNGTC 1 cut(s) 85
PflMI CCANNNNNTGG 1 cut(s) 2090
PfoI TCCNGGA 3 cut(s) 937, 1236, 3125
PleI GAGTC 5 cut(s) 2, 1262, 1518, 1576, 3355
PpsI GAGTC 5 cut(s) 2, 1262, 1518, 1576, 3355
PpuMI RGGWCCY 1 cut(s) 3266
PshBI ATTAAT 1 cut(s) 1703
Psp5II RGGWCCY 1 cut(s) 3266
Psp6I CCWGG 8 cut(s) 530, 937, 1084, 1236, 2089, 2151, 2248, 2786
PspEI GGTNACC 1 cut(s) 2056
PspFI CCCAGC 3 cut(s) 946, 1244, 3368
PspGI CCWGG 8 cut(s) 530, 937, 1084, 1236, 2089, 2151, 2248, 2786
PspPI GGNCC 7 cut(s) 46, 201, 821, 2024, 2750, 3266, 3583
PspPPI RGGWCCY 1 cut(s) 3266
PstNI CAGNNNCTG 3 cut(s) 548, 1085, 2876
PsyI GACNNNGTC 1 cut(s) 85
PvuII CAGCTG 3 cut(s) 2828, 2876, 3491
RsaI GTAC 8 cut(s) 651, 959, 1221, 1470, 1986, 2172, 2254, 3515
RsaNI GTAC 8 cut(s) 650, 958, 1220, 1469, 1985, 2171, 2253, 3514
RseI CAYNNNNRTG 5 cut(s) 287, 1116, 1735, 2402, 2631
SapI GCTCTTC 2 cut(s) 2652, 3300
Sau96I GGNCC 7 cut(s) 46, 201, 821, 2024, 2750, 3266, 3583
ScaI AGTACT 1 cut(s) 1470
SchI GAGTC 5 cut(s) 2, 1263, 1519, 1576, 3356
SduI GDGCHC 4 cut(s) 556, 916, 1174, 2596
SfcI CTRYAG 4 cut(s) 675, 2339, 2460, 2877
SinI GGWCC 5 cut(s) 46, 201, 2024, 3266, 3583
SmaI CCCGGG 2 cut(s) 1965, 3556
SmiMI CAYNNNNRTG 5 cut(s) 287, 1116, 1735, 2402, 2631
SmlI CTYRAG 4 cut(s) 803, 1355, 2388, 2663
SmoI CTYRAG 4 cut(s) 803, 1355, 2388, 2663
SphI GCATGC 3 cut(s) 1010, 1734, 1892
SseBI AGGCCT 2 cut(s) 1192, 2541
SspI AATATT 1 cut(s) 2797
StuI AGGCCT 2 cut(s) 1192, 2541
StyI CCWWGG 3 cut(s) 2521, 2542, 2929
TaaI ACNGT 5 cut(s) 58, 962, 1726, 1998, 2946
TaiI ACGT 2 cut(s) 86, 414
TaqII GACCGA 1 cut(s) 3145
TatI WGTACW 4 cut(s) 1219, 1468, 1984, 2170
TauI GCSGC 3 cut(s) 134, 251, 1523
TscAI CASTG 5 cut(s) 491, 1563, 2047, 2362, 3463
TseFI GTSAC 1 cut(s) 1921
Tsp45I GTSAC 1 cut(s) 1921
TspGWI ACGGA 1 cut(s) 2955
TspMI CCCGGG 2 cut(s) 1963, 3554
TspRI CASTG 5 cut(s) 491, 1563, 2047, 2362, 3463
Tth111I GACNNNGTC 1 cut(s) 85
Van91I CCANNNNNTGG 1 cut(s) 2090
Vha464I CTTAAG 1 cut(s) 2663
VneI GTGCAC 1 cut(s) 552
VpaK11BI GGWCC 5 cut(s) 46, 201, 2024, 3266, 3583
VspI ATTAAT 1 cut(s) 1703
XapI RAATTY 8 cut(s) 701, 757, 765, 1017, 2447, 2841, 2984, 3481
XceI RCATGY 6 cut(s) 1010, 1079, 1565, 1734, 1892, 2500
XcmI CCANNNNNNNNNTGG 2 cut(s) 591, 2528
XmaI CCCGGG 2 cut(s) 1963, 3554
XmiI GTMKAC 1 cut(s) 432
XmnI GAANNNNTTC 2 cut(s) 1456, 1645
ZraI GACGTC 1 cut(s) 84
ZrmI AGTACT 1 cut(s) 1470
Zsp2I ATGCAT 1 cut(s) 2502
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.