Rmu_sc0003825.1_g000002

NDH shuttles electrons from NAD(P)H plastoquinone, via FMN and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003825.1
OG0006041
Physical Location & Seq
Forward (+)
11584 .. 13696
2113 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 339 bp
atgctgggagcgtccggaataccatgggatcttcgtaaagttgatcagtatgagtgttatgacgaatttgattgggaaacctgcctgcaacaagccgctgagaagacagcctccggtcaccagtccggcggccagccctggtcctccaccattgccaatctggtaaggagcaactggacccgacccggaacaaacaccgcaagccacaaaaaaacgatcaacggagcccccttacctcagccgcctactgagaacctgcacccactcttgaagccgctcgactacacaccacagtcgatttctcagatgccaatctgcaccaacccagaggacgcctag

Protein Analysis

112

Amino Acids

12.38

Weight (kDa)

5.17

Isoelectric Point (pI)

52.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Complex1_49kDa PF00346 1 - 33 2.5e-07 Respiratory-chain NADH dehydrogenase, 49 Kd subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 89, 264
AccBSI CCGCTC 1 cut(s) 277
AccIII TCCGGA 1 cut(s) 14
AciI CCGC 5 cut(s) 96, 129, 198, 242, 275
AclWI GGATC 1 cut(s) 36
AcoI YGGCCR 1 cut(s) 130
AcsI RAATTY 1 cut(s) 65
AcyI GRCGYC 1 cut(s) 333
AgsI TTSAA 1 cut(s) 271
AjnI CCWGG 1 cut(s) 137
AlwI GGATC 1 cut(s) 36
Aor13HI TCCGGA 1 cut(s) 14
AoxI GGCC 1 cut(s) 130
ApoI RAATTY 1 cut(s) 65
AspS9I GGNCC 2 cut(s) 141, 177
AsuC2I CCSGG 1 cut(s) 186
AsuHPI GGTGA 1 cut(s) 110
AvaII GGWCC 2 cut(s) 141, 177
BanII GRGCYC 1 cut(s) 229
BbsI GAAGAC 1 cut(s) 110
BbvCI CCTCAGC 1 cut(s) 237
BciT130I CCWGG 1 cut(s) 139
BclI TGATCA 1 cut(s) 43
BcnI CCSGG 1 cut(s) 186
BfaI CTAG 1 cut(s) 337
BfuAI ACCTGC 2 cut(s) 89, 264
BisI GCNGC 4 cut(s) 96, 130, 242, 275
BlsI GCNGC 4 cut(s) 97, 131, 243, 276
Bme1390I CCNGG 2 cut(s) 139, 186
Bme18I GGWCC 2 cut(s) 141, 177
BmgT120I GGNCC 2 cut(s) 141, 177
BmiI GGNNCC 2 cut(s) 179, 226
BmrFI CCNGG 2 cut(s) 139, 186
BmsI GCATC 1 cut(s) 297
BpiI GAAGAC 1 cut(s) 110
Bpu10I CCTNAGC 1 cut(s) 237
BpuMI CCSGG 1 cut(s) 186
BsaBI GATNNNNATC 1 cut(s) 311
BsaHI GRCGYC 1 cut(s) 333
BsaJI CCNNGG 2 cut(s) 23, 137
BsaWI WCCGGW 2 cut(s) 14, 113
Bse1I ACTGG 2 cut(s) 121, 179
Bse3DI GCAATG 1 cut(s) 150
Bse8I GATNNNNATC 1 cut(s) 311
BseAI TCCGGA 1 cut(s) 14
BseBI CCWGG 1 cut(s) 139
BseDI CCNNGG 2 cut(s) 23, 137
BseJI GATNNNNATC 1 cut(s) 311
BseMI GCAATG 1 cut(s) 150
BseMII CTCAG 4 cut(s) 90, 240, 251, 317
BseNI ACTGG 2 cut(s) 121, 179
BseYI CCCAGC 1 cut(s) 4
BsgI GTGCAG 2 cut(s) 242, 301
BshFI GGCC 1 cut(s) 132
BsiSI CCGG 4 cut(s) 15, 114, 126, 186
BsnI GGCC 1 cut(s) 132
Bsp1286I GDGCHC 1 cut(s) 229
Bsp13I TCCGGA 1 cut(s) 14
Bsp143I GATC 3 cut(s) 28, 43, 216
Bsp19I CCATGG 1 cut(s) 23
BspACI CCGC 5 cut(s) 96, 129, 198, 242, 275
BspANI GGCC 1 cut(s) 132
BspCNI CTCAG 4 cut(s) 91, 241, 250, 316
BspEI TCCGGA 1 cut(s) 14
BspLI GGNNCC 2 cut(s) 179, 226
BspMI ACCTGC 2 cut(s) 89, 264
BspPI GGATC 1 cut(s) 36
BsrBI CCGCTC 1 cut(s) 277
BsrDI GCAATG 1 cut(s) 150
BsrI ACTGG 2 cut(s) 121, 179
BssECI CCNNGG 2 cut(s) 23, 137
BssMI GATC 3 cut(s) 28, 43, 216
BssNI GRCGYC 1 cut(s) 333
BssT1I CCWWGG 1 cut(s) 23
Bst2UI CCWGG 1 cut(s) 139
Bst4CI ACNGT 1 cut(s) 294
BstACI GRCGYC 1 cut(s) 333
BstC8I GCNNGC 3 cut(s) 86, 134, 202
BstDEI CTNAG 4 cut(s) 99, 237, 249, 303
BstDSI CCRYGG 1 cut(s) 23
BstEII GGTNACC 1 cut(s) 116
BstKTI GATC 3 cut(s) 31, 46, 219
BstMBI GATC 3 cut(s) 28, 43, 216
BstNI CCWGG 1 cut(s) 139
BstPI GGTNACC 1 cut(s) 116
BstSCI CCNGG 2 cut(s) 137, 184
BstV2I GAAGAC 1 cut(s) 110
BstX2I RGATCY 1 cut(s) 28
BstYI RGATCY 1 cut(s) 28
BsuRI GGCC 1 cut(s) 132
BtgI CCRYGG 1 cut(s) 23
BveI ACCTGC 2 cut(s) 89, 264
Cac8I GCNNGC 3 cut(s) 86, 134, 202
Cfr13I GGNCC 2 cut(s) 141, 177
CviAII CATG 1 cut(s) 24
CviJI RGCY 8 cut(s) 95, 110, 132, 136, 204, 227, 241, 274
CviKI_1 RGCY 8 cut(s) 95, 110, 132, 136, 204, 227, 241, 274
DdeI CTNAG 4 cut(s) 99, 237, 249, 303
DpnI GATC 3 cut(s) 30, 45, 218
DpnII GATC 3 cut(s) 28, 43, 216
EaeI YGGCCR 1 cut(s) 130
Eco130I CCWWGG 1 cut(s) 23
Eco24I GRGCYC 1 cut(s) 229
Eco47I GGWCC 2 cut(s) 141, 177
Eco91I GGTNACC 1 cut(s) 116
EcoO65I GGTNACC 1 cut(s) 116
EcoRII CCWGG 1 cut(s) 137
EcoT14I CCWWGG 1 cut(s) 23
EcoT38I GRGCYC 1 cut(s) 229
ErhI CCWWGG 1 cut(s) 23
FaeI CATG 1 cut(s) 27
FaiI YATR 3 cut(s) 25, 51, 60
FatI CATG 1 cut(s) 23
FbaI TGATCA 1 cut(s) 43
Fnu4HI GCNGC 4 cut(s) 96, 130, 242, 275
FriOI GRGCYC 1 cut(s) 229
Fsp4HI GCNGC 4 cut(s) 96, 130, 242, 275
FspBI CTAG 1 cut(s) 337
GluI GCNGC 4 cut(s) 96, 130, 242, 275
GsaI CCCAGC 1 cut(s) 8
HaeIII GGCC 1 cut(s) 132
HapII CCGG 4 cut(s) 15, 114, 126, 186
Hin1I GRCGYC 1 cut(s) 333
Hin1II CATG 1 cut(s) 27
HpaII CCGG 4 cut(s) 15, 114, 126, 186
HphI GGTGA 1 cut(s) 110
Hpy188I TCNGA 1 cut(s) 306
Hpy188III TCNNGA 2 cut(s) 15, 268
HpyCH4III ACNGT 1 cut(s) 294
HpyCH4V TGCA 3 cut(s) 88, 259, 318
HpyF3I CTNAG 4 cut(s) 99, 237, 249, 303
Hsp92I GRCGYC 1 cut(s) 333
Hsp92II CATG 1 cut(s) 27
Kpn2I TCCGGA 1 cut(s) 14
Ksp22I TGATCA 1 cut(s) 43
Kzo9I GATC 3 cut(s) 28, 43, 216
LmnI GCTCC 3 cut(s) 8, 168, 224
LweI GCATC 1 cut(s) 297
MaeI CTAG 1 cut(s) 337
MaeIII GTNAC 1 cut(s) 116
MalI GATC 3 cut(s) 30, 45, 218
MbiI CCGCTC 1 cut(s) 277
MboI GATC 3 cut(s) 28, 43, 216
MboII GAAGA 2 cut(s) 23, 115
MflI RGATCY 1 cut(s) 28
MhlI GDGCHC 1 cut(s) 229
MluCI AATT 1 cut(s) 65
MnlI CCTC 4 cut(s) 121, 154, 246, 322
MroI TCCGGA 1 cut(s) 14
MspA1I CMGCKG 1 cut(s) 98
MspI CCGG 4 cut(s) 15, 114, 126, 186
MspR9I CCNGG 2 cut(s) 139, 186
MvaI CCWGG 1 cut(s) 139
NciI CCSGG 1 cut(s) 186
NcoI CCATGG 1 cut(s) 23
NdeII GATC 3 cut(s) 28, 43, 216
NlaIII CATG 1 cut(s) 27
NlaIV GGNNCC 2 cut(s) 179, 226
NmuCI GTSAC 1 cut(s) 116
PkrI GCNGC 4 cut(s) 97, 131, 243, 276
Psp6I CCWGG 1 cut(s) 137
PspEI GGTNACC 1 cut(s) 116
PspFI CCCAGC 1 cut(s) 4
PspGI CCWGG 1 cut(s) 137
PspN4I GGNNCC 2 cut(s) 179, 226
PspPI GGNCC 2 cut(s) 141, 177
PsuI RGATCY 1 cut(s) 28
SatI GCNGC 4 cut(s) 96, 130, 242, 275
Sau3AI GATC 3 cut(s) 28, 43, 216
Sau96I GGNCC 2 cut(s) 141, 177
ScrFI CCNGG 2 cut(s) 139, 186
SduI GDGCHC 1 cut(s) 229
SetI ASST 3 cut(s) 83, 238, 258
SfaNI GCATC 1 cut(s) 297
SinI GGWCC 2 cut(s) 141, 177
Sse9I AATT 1 cut(s) 65
SsiI CCGC 5 cut(s) 96, 129, 198, 242, 275
SspMI CTAG 1 cut(s) 337
StyD4I CCNGG 2 cut(s) 137, 184
StyI CCWWGG 1 cut(s) 23
TaaI ACNGT 1 cut(s) 294
TaqI TCGA 2 cut(s) 279, 296
TasI AATT 1 cut(s) 65
TauI GCSGC 4 cut(s) 98, 132, 244, 277
TseFI GTSAC 1 cut(s) 116
Tsp45I GTSAC 1 cut(s) 116
TspGWI ACGGA 1 cut(s) 237
VpaK11BI GGWCC 2 cut(s) 141, 177
XapI RAATTY 1 cut(s) 65
XcmI CCANNNNNNNNNTGG 1 cut(s) 157
XspI CTAG 1 cut(s) 337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.