Rmu_sc0003837.1_g000016

Arabidopsis phospholipase-like protein (PEARLI 4)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003837.1
OG0002091
Physical Location & Seq
Reverse (-)
87040 .. 89530
2491 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1473 bp
atggctggagagagaagagtggatgcaactagggcatctaatccttaccatcaatctggtcaatcttttccgaaaagaactgccggtggtgaagctcgtggggatataaaagaatcaggctccatacttgatgttcctatattgggtggacgaaaagagagatcccgaccaaagactcctcaagatcttgacaatgtatctgaccgaagtgcggtttatccttatgatgctcaatcatctccatctaaggacaaggtgaacgtagagaatgggtctgtccttgatgatcctatatttggaaaaagaaagccagtctcccaaacaaggtctgctgaagagcatgaaaatggtcaaggtgttgatccttcatcatttccagaaaagctgaaaatagagaacggctctgcccttgatgatcctatatttggcagaagaaagcaggtagcccagccaatgccccatgaagagcttgacaatggatccggtcaaggttcagttaatccttctggttctcccatgtctccatctaatgaaattgtgaaaatagggaatggctccactcttgatgatcttatatttggccgcaaaaagcagggatctgaaccaaagcctgaagaagagattgagaatgtacctgttgtgaaattagataatgatgagtataagtcttattctggaccgcttgctcaagtaaaagatccttctttgaacaagaagcaaatcaattcctctggggcagtgcctgcatctggaattatcaaaatgaccccctctctttctctccccacacacaatgttggagaaggtcccactgccacaccttctgaaaacagaagcttcagtttctcaggttctccctatgtttttgaagagagtgatgatgacgatgcacgatctgtcaattctgatgccagtgtttctgtgggaaagtaccgtgtgaaaggaagcttatgttccattctccagtcaatctttgacaagtatggtgatatagcagcaagctgtcaattggaatcaattgtcattcgctcttattacctcgagtgtgtctgttatgtggttcaagagttgaaacgcacttcggtgaagcagctgacaaagtcaaaagtcaaagaaatgtcagctatgctcaaggatgtagaatcttcaggaatggatgttggttggcttcgtctcatgctcaatgaatgtgcacaatccattgatctcgtgagtcagcatcgagcattcgaactagaaaaggccaactgtgatcgtggcattgaatcaataaggaaagaactagagtcccagatggaggctttggctctgaaagaaaaggaggttgctgatgccaagaagcaagtttctgaaaccagagcccatctgagggagcttgagctcaaatcatcttttttgagtgagactgttagtaatctgaagtccaaggttgaaaatcttcaaagcaaatcattgctggacaaggtcttgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PEARLI-4

Protein Analysis

490

Amino Acids

53.58

Weight (kDa)

5.59

Isoelectric Point (pI)

56.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PEARLI-4 PF05278 295 - 487 2e-64 Arabidopsis phospholipase-like protein (PEARLI 4)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 430
AciI CCGC 3 cut(s) 212, 583, 680
AclWI GGATC 8 cut(s) 156, 281, 356, 410, 474, 487, 604, 692
AcoI YGGCCR 1 cut(s) 580
AcuI CTGAAG 5 cut(s) 354, 633, 823, 1131, 1439
AfaI GTAC 2 cut(s) 633, 932
AfiI CCNNNNNNNGG 9 cut(s) 143, 211, 296, 324, 425, 749, 1297, 1368, 1369
AgsI TTSAA 7 cut(s) 709, 869, 1064, 1072, 1265, 1433, 1442
AleI CACNNNNGTG 1 cut(s) 1082
Alw21I GWGCWC 2 cut(s) 1195, 1383
Alw26I GTCTC 4 cut(s) 319, 525, 1178, 1397
Alw44I GTGCAC 1 cut(s) 1191
AlwI GGATC 8 cut(s) 156, 281, 356, 410, 474, 487, 604, 692
AlwNI CAGNNNCTG 1 cut(s) 743
Ama87I CYCGRG 1 cut(s) 1040
AoxI GGCC 2 cut(s) 580, 1242
ApaLI GTGCAC 1 cut(s) 1191
ApeKI GCWGC 2 cut(s) 995, 1090
Asp700I GAANNNNTTC 1 cut(s) 1437
AspS9I GGNCC 2 cut(s) 677, 806
AsuHPI GGTGA 4 cut(s) 101, 268, 998, 1096
AsuII TTCGAA 1 cut(s) 1230
AvaI CYCGRG 1 cut(s) 1040
AvaII GGWCC 2 cut(s) 677, 806
BaeGI GKGCMC 1 cut(s) 1195
BamHI GGATCC 1 cut(s) 479
BanII GRGCYC 2 cut(s) 1363, 1383
BauI CACGAG 2 cut(s) 96, 1208
Bbv12I GWGCWC 2 cut(s) 1195, 1383
BbvI GCAGC 2 cut(s) 1007, 1102
BccI CCATC 5 cut(s) 57, 250, 532, 1288, 1371
BceAI ACGGC 1 cut(s) 415
BcoDI GTCTC 4 cut(s) 319, 525, 1178, 1397
BfaI CTAG 3 cut(s) 30, 1235, 1283
BfuAI ACCTGC 1 cut(s) 430
BglII AGATCT 1 cut(s) 184
BisI GCNGC 3 cut(s) 583, 996, 1091
BlsI GCNGC 3 cut(s) 584, 997, 1092
Bme18I GGWCC 2 cut(s) 677, 806
BmeT110I CYCGRG 1 cut(s) 1040
BmgT120I GGNCC 2 cut(s) 677, 806
BmiI GGNNCC 4 cut(s) 121, 481, 556, 808
BmsI GCATC 8 cut(s) 13, 44, 217, 755, 877, 898, 1228, 1321
BplI GAGNNNNNCTC 2 cut(s) 386, 418
BpmI CTGGAG 2 cut(s) 27, 947
Bpu14I TTCGAA 1 cut(s) 1230
BpuEI CTTGAG 4 cut(s) 165, 672, 1115, 1397
BsaJI CCNNGG 1 cut(s) 1425
BsaWI WCCGGW 1 cut(s) 482
Bsc4I CCNNNNNNNGG 9 cut(s) 143, 211, 296, 324, 425, 749, 1297, 1368, 1369
Bse118I RCCGGY 1 cut(s) 83
Bse1I ACTGG 3 cut(s) 311, 912, 964
Bse3DI GCAATG 1 cut(s) 1451
BseDI CCNNGG 1 cut(s) 1425
BseGI GGATG 3 cut(s) 28, 1141, 1162
BseLI CCNNNNNNNGG 9 cut(s) 143, 211, 296, 324, 425, 749, 1297, 1368, 1369
BseMI GCAATG 1 cut(s) 1451
BseMII CTCAG 2 cut(s) 861, 1358
BseNI ACTGG 3 cut(s) 311, 912, 964
BseRI GAGGAG 1 cut(s) 168
BseSI GKGCMC 1 cut(s) 1195
BseXI GCAGC 2 cut(s) 1007, 1102
BseYI CCCAGC 1 cut(s) 447
BshFI GGCC 2 cut(s) 582, 1244
BsiHKAI GWGCWC 2 cut(s) 1195, 1383
BsiHKCI CYCGRG 1 cut(s) 1040
BsiSI CCGG 2 cut(s) 84, 483
BslFI GGGAC 2 cut(s) 792, 1273
BslI CCNNNNNNNGG 9 cut(s) 143, 211, 296, 324, 425, 749, 1297, 1368, 1369
BsmAI GTCTC 4 cut(s) 319, 525, 1178, 1397
BsmBI CGTCTC 1 cut(s) 1178
BsmFI GGGAC 2 cut(s) 792, 1273
BsmI GAATGC 1 cut(s) 1226
BsnI GGCC 2 cut(s) 582, 1244
BsoBI CYCGRG 1 cut(s) 1040
Bsp119I TTCGAA 1 cut(s) 1230
Bsp1286I GDGCHC 3 cut(s) 1195, 1363, 1383
BspACI CCGC 3 cut(s) 212, 583, 680
BspANI GGCC 2 cut(s) 582, 1244
BspCNI CTCAG 2 cut(s) 860, 1359
BspLI GGNNCC 4 cut(s) 121, 481, 556, 808
BspMI ACCTGC 1 cut(s) 430
BspPI GGATC 8 cut(s) 156, 281, 356, 410, 474, 487, 604, 692
BspQI GCTCTTC 2 cut(s) 330, 459
BspT104I TTCGAA 1 cut(s) 1230
BsrDI GCAATG 1 cut(s) 1451
BsrFI RCCGGY 1 cut(s) 83
BsrI ACTGG 3 cut(s) 311, 912, 964
BssAI RCCGGY 1 cut(s) 83
BssECI CCNNGG 1 cut(s) 1425
BssSI CACGAG 2 cut(s) 96, 1208
BssT1I CCWWGG 1 cut(s) 1425
Bst2BI CACGAG 2 cut(s) 96, 1208
Bst4CI ACNGT 3 cut(s) 935, 1250, 1408
Bst6I CTCTTC 5 cut(s) 10, 330, 459, 612, 864
BstAPI GCANNNNNTGC 1 cut(s) 743
BstBI TTCGAA 1 cut(s) 1230
BstC8I GCNNGC 3 cut(s) 684, 744, 1000
BstDEI CTNAG 3 cut(s) 246, 847, 1367
BstF5I GGATG 3 cut(s) 28, 1141, 1162
BstMAI GTCTC 4 cut(s) 319, 525, 1178, 1397
BstMWI GCNNNNNNNGC 2 cut(s) 32, 743
BstSLI GKGCMC 1 cut(s) 1195
BstV1I GCAGC 2 cut(s) 1007, 1102
BstX2I RGATCY 5 cut(s) 161, 184, 479, 596, 697
BstXI CCANNNNNNTGG 1 cut(s) 56
BstYI RGATCY 5 cut(s) 161, 184, 479, 596, 697
BsuRI GGCC 2 cut(s) 582, 1244
BtsCI GGATG 3 cut(s) 28, 1141, 1162
BtsI GCAGTG 2 cut(s) 744, 810
BtsIMutI CAGTG 3 cut(s) 744, 810, 919
BveI ACCTGC 1 cut(s) 430
Cac8I GCNNGC 3 cut(s) 684, 744, 1000
CaiI CAGNNNCTG 1 cut(s) 743
Cfr10I RCCGGY 1 cut(s) 83
Cfr13I GGNCC 2 cut(s) 677, 806
Csp6I GTAC 2 cut(s) 632, 931
CviAII CATG 4 cut(s) 341, 461, 517, 1177
CviQI GTAC 2 cut(s) 632, 931
DdeI CTNAG 3 cut(s) 246, 847, 1367
EaeI YGGCCR 1 cut(s) 580
Eam1104I CTCTTC 5 cut(s) 10, 330, 459, 612, 864
EarI CTCTTC 5 cut(s) 10, 330, 459, 612, 864
Ecl136II GAGCTC 1 cut(s) 1381
Eco130I CCWWGG 1 cut(s) 1425
Eco24I GRGCYC 2 cut(s) 1363, 1383
Eco47I GGWCC 2 cut(s) 677, 806
Eco53kI GAGCTC 1 cut(s) 1381
Eco57I CTGAAG 5 cut(s) 354, 633, 823, 1131, 1439
Eco88I CYCGRG 1 cut(s) 1040
EcoICRI GAGCTC 1 cut(s) 1381
EcoO109I RGGNCCY 1 cut(s) 806
EcoT14I CCWWGG 1 cut(s) 1425
EcoT38I GRGCYC 2 cut(s) 1363, 1383
ErhI CCWWGG 1 cut(s) 1425
Esp3I CGTCTC 1 cut(s) 1178
FaeI CATG 4 cut(s) 344, 464, 520, 1180
FaqI GGGAC 2 cut(s) 792, 1273
FatI CATG 4 cut(s) 340, 460, 516, 1176
Fnu4HI GCNGC 3 cut(s) 583, 996, 1091
FokI GGATG 3 cut(s) 35, 1148, 1169
FriOI GRGCYC 2 cut(s) 1363, 1383
Fsp4HI GCNGC 3 cut(s) 583, 996, 1091
FspBI CTAG 3 cut(s) 30, 1235, 1283
GluI GCNGC 3 cut(s) 583, 996, 1091
GsaI CCCAGC 1 cut(s) 451
GsuI CTGGAG 2 cut(s) 27, 947
HaeIII GGCC 2 cut(s) 582, 1244
HapII CCGG 2 cut(s) 84, 483
Hin1II CATG 4 cut(s) 344, 464, 520, 1180
HindIII AAGCTT 2 cut(s) 835, 946
HinfI GANTC 7 cut(s) 113, 175, 1013, 1142, 1213, 1265, 1286
HpaII CCGG 2 cut(s) 84, 483
HphI GGTGA 4 cut(s) 101, 268, 998, 1096
Hpy166II GTNNAC 3 cut(s) 149, 259, 1193
Hpy188I TCNGA 9 cut(s) 72, 202, 601, 826, 907, 1311, 1351, 1368, 1419
Hpy8I GTNNAC 3 cut(s) 149, 259, 1193
HpyAV CCTTC 5 cut(s) 375, 513, 711, 797, 831
HpyCH4III ACNGT 3 cut(s) 935, 1250, 1408
HpyCH4IV ACGT 1 cut(s) 261
HpyCH4V TGCA 4 cut(s) 26, 746, 890, 1193
HpyF10VI GCNNNNNNNGC 2 cut(s) 32, 743
HpyF3I CTNAG 3 cut(s) 246, 847, 1367
HpySE526I ACGT 1 cut(s) 261
Hsp92II CATG 4 cut(s) 344, 464, 520, 1180
LguI GCTCTTC 2 cut(s) 330, 459
LmnI GCTCC 3 cut(s) 125, 560, 1372
Lsp1109I GCAGC 2 cut(s) 1007, 1102
LweI GCATC 8 cut(s) 13, 44, 217, 755, 877, 898, 1228, 1321
MaeI CTAG 3 cut(s) 30, 1235, 1283
MaeII ACGT 1 cut(s) 261
MboII GAAGA 9 cut(s) 27, 347, 444, 476, 626, 629, 881, 1137, 1430
MfeI CAATTG 2 cut(s) 1007, 1017
MflI RGATCY 5 cut(s) 161, 184, 479, 596, 697
MhlI GDGCHC 3 cut(s) 1195, 1363, 1383
MluCI AATT 7 cut(s) 534, 644, 724, 753, 901, 1007, 1017
MlyI GAGTC 3 cut(s) 169, 1222, 1295
MmeI TCCRAC 1 cut(s) 778
MnlI CCTC 7 cut(s) 189, 739, 781, 1049, 1291, 1315, 1362
MroXI GAANNNNTTC 1 cut(s) 1437
MseI TTAA 1 cut(s) 498
MslI CAYNNNNRTG 2 cut(s) 345, 1082
MspA1I CMGCKG 1 cut(s) 1093
MspI CCGG 2 cut(s) 84, 483
MunI CAATTG 2 cut(s) 1007, 1017
Mva1269I GAATGC 1 cut(s) 1226
MwoI GCNNNNNNNGC 2 cut(s) 32, 743
NlaIII CATG 4 cut(s) 344, 464, 520, 1180
NlaIV GGNNCC 4 cut(s) 121, 481, 556, 808
NspV TTCGAA 1 cut(s) 1230
OliI CACNNNNGTG 1 cut(s) 1082
PaeR7I CTCGAG 1 cut(s) 1040
PciSI GCTCTTC 2 cut(s) 330, 459
PctI GAATGC 1 cut(s) 1226
PdmI GAANNNNTTC 1 cut(s) 1437
PfeI GAWTC 4 cut(s) 113, 1013, 1142, 1265
PflFI GACNNNGTC 2 cut(s) 1099, 1463
PkrI GCNGC 3 cut(s) 584, 997, 1092
PleI GAGTC 3 cut(s) 169, 1221, 1294
PpsI GAGTC 3 cut(s) 169, 1221, 1294
PpuMI RGGWCCY 1 cut(s) 806
Psp124BI GAGCTC 1 cut(s) 1383
Psp5II RGGWCCY 1 cut(s) 806
PspFI CCCAGC 1 cut(s) 447
PspN4I GGNNCC 4 cut(s) 121, 481, 556, 808
PspPI GGNCC 2 cut(s) 677, 806
PspPPI RGGWCCY 1 cut(s) 806
PspXI VCTCGAGB 1 cut(s) 1040
PstNI CAGNNNCTG 1 cut(s) 743
PsuI RGATCY 5 cut(s) 161, 184, 479, 596, 697
PsyI GACNNNGTC 2 cut(s) 1099, 1463
PvuII CAGCTG 1 cut(s) 1093
RsaI GTAC 2 cut(s) 633, 932
RsaNI GTAC 2 cut(s) 632, 931
RseI CAYNNNNRTG 2 cut(s) 345, 1082
SacI GAGCTC 1 cut(s) 1383
SapI GCTCTTC 2 cut(s) 330, 459
SaqAI TTAA 1 cut(s) 498
SatI GCNGC 3 cut(s) 583, 996, 1091
Sau96I GGNCC 2 cut(s) 677, 806
SchI GAGTC 3 cut(s) 169, 1222, 1295
SduI GDGCHC 3 cut(s) 1195, 1363, 1383
SfaNI GCATC 8 cut(s) 13, 44, 217, 755, 877, 898, 1228, 1321
Sfr274I CTCGAG 1 cut(s) 1040
SfuI TTCGAA 1 cut(s) 1230
SinI GGWCC 2 cut(s) 677, 806
SlaI CTCGAG 1 cut(s) 1040
SmiMI CAYNNNNRTG 2 cut(s) 345, 1082
SmlI CTYRAG 5 cut(s) 180, 687, 1040, 1130, 1376
SmoI CTYRAG 5 cut(s) 180, 687, 1040, 1130, 1376
Sse9I AATT 7 cut(s) 534, 644, 724, 753, 901, 1007, 1017
SsiI CCGC 3 cut(s) 212, 583, 680
SspMI CTAG 3 cut(s) 30, 1235, 1283
SstI GAGCTC 1 cut(s) 1383
StyI CCWWGG 1 cut(s) 1425
TaaI ACNGT 3 cut(s) 935, 1250, 1408
TaiI ACGT 1 cut(s) 264
TaqI TCGA 3 cut(s) 1041, 1222, 1230
TaqII GACCGA 1 cut(s) 219
TasI AATT 7 cut(s) 534, 644, 724, 753, 901, 1007, 1017
TauI GCSGC 1 cut(s) 585
TfiI GAWTC 4 cut(s) 113, 1013, 1142, 1265
Tru1I TTAA 1 cut(s) 498
Tru9I TTAA 1 cut(s) 498
TscAI CASTG 3 cut(s) 744, 817, 919
TseI GCWGC 2 cut(s) 995, 1090
TspDTI ATGAA 5 cut(s) 357, 357, 477, 546, 1200
TspRI CASTG 3 cut(s) 744, 817, 919
Tth111I GACNNNGTC 2 cut(s) 1099, 1463
VneI GTGCAC 1 cut(s) 1191
VpaK11BI GGWCC 2 cut(s) 677, 806
XcmI CCANNNNNNNNNTGG 1 cut(s) 919
XhoI CTCGAG 1 cut(s) 1040
XmnI GAANNNNTTC 1 cut(s) 1437
XspI CTAG 3 cut(s) 30, 1235, 1283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.