Rmu_sc0004154.1_g000008

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004154.1
OG0010776
Physical Location & Seq
Reverse (-)
23423 .. 28480
5058 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004154.1_g000008.1.cds

Sequence Viewer

Length: 861 bp
atgttcatctacgattcacttgatgacttcacaatattactcaactgctcctctaagaatacagtactacatgacattccaccggtcacttgcctcgacgatgattatatcagctacatggtcattgctgtgccgcgtagtgcagtgccggcggaccactacggatgctctgtgatttcaacagctctcgctccgaagcatcttgatttgggtgtcgagttgacatgggacaaacctgactgtagtttatgtgaaaacaaagctcttctttgtggactggagcatggtaaaaagttccagtatacggtgctcctctctttctacggaaaccgcactgcaatttctatagtttcaggtgatctctcatcaagagctataaagtttggcctagtgctaagcgcgggaattcttgtagtattgttcatatatactttgtttgtgtctggtctcgtggccagccgccagcccacaatcactgaactatccaccatagccaccggacaactgcagcttccggaagctgaaaggggcctccatcgttactcgacggacaggcatcggaagctttctttctttgttgtattcgtcgtcgtcgtcgtctccggcagaagagaagagagagagatgggtggagcccagttgatgaagcgaatccctcgcatcaaatttcctcagagacatccaaagcccgctggttctgaatcccagacagcagcacctttggctggagattctgttcaagctttcttctcaagttcaaaggcttcaacaactttgggaggaaaagcttctgaacaacccccacgaacaccagtatccaaagaagagattgaggctattttgttgggtggctgcatctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind zf-RING_2

Protein Analysis

286

Amino Acids

31.16

Weight (kDa)

7.61

Isoelectric Point (pI)

42.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 302
AccII CGCG 2 cut(s) 136, 401
AccIII TCCGGA 1 cut(s) 514
AciI CCGC 6 cut(s) 134, 152, 331, 401, 460, 690
AcoI YGGCCR 1 cut(s) 453
AcsI RAATTY 2 cut(s) 405, 665
AfaI GTAC 1 cut(s) 66
AfiI CCNNNNNNNGG 2 cut(s) 304, 725
AgeI ACCGGT 1 cut(s) 82
AgsI TTSAA 4 cut(s) 180, 740, 759, 768
AloI GAACNNNNNNTCC 2 cut(s) 720, 752
AluBI AGCT 9 cut(s) 114, 185, 263, 374, 511, 521, 565, 743, 788
AluI AGCT 9 cut(s) 114, 185, 263, 374, 511, 521, 565, 743, 788
Alw21I GWGCWC 1 cut(s) 312
Alw26I GTCTC 3 cut(s) 452, 604, 670
Aor13HI TCCGGA 1 cut(s) 514
AoxI GGCC 3 cut(s) 385, 453, 529
ApeKI GCWGC 3 cut(s) 508, 713, 852
ApoI RAATTY 2 cut(s) 405, 665
AsiGI ACCGGT 1 cut(s) 82
Asp700I GAANNNNTTC 1 cut(s) 787
AspLEI GCGC 1 cut(s) 401
AspS9I GGNCC 2 cut(s) 154, 529
AsuHPI GGTGA 1 cut(s) 368
AvaII GGWCC 1 cut(s) 154
BaeI ACNNNNGTAYC 2 cut(s) 798, 831
BalI TGGCCA 1 cut(s) 455
BanII GRGCYC 1 cut(s) 637
BauI CACGAG 1 cut(s) 449
Bbv12I GWGCWC 1 cut(s) 312
BbvI GCAGC 3 cut(s) 520, 725, 839
BccI CCATC 2 cut(s) 543, 619
BciVI GTATCC 1 cut(s) 826
BcoDI GTCTC 3 cut(s) 452, 604, 670
BfaI CTAG 1 cut(s) 389
BfmI CTRYAG 3 cut(s) 241, 345, 506
BfuI GTATCC 1 cut(s) 826
BisI GCNGC 5 cut(s) 134, 460, 509, 714, 853
BlpI GCTNAGC 1 cut(s) 395
BlsI GCNGC 5 cut(s) 135, 461, 510, 715, 854
BmcAI AGTACT 1 cut(s) 66
Bme18I GGWCC 1 cut(s) 154
BmgT120I GGNCC 2 cut(s) 154, 529
BmiI GGNNCC 2 cut(s) 530, 634
BmrI ACTGGG 1 cut(s) 631
BmsI GCATC 4 cut(s) 155, 208, 565, 669
BmuI ACTGGG 1 cut(s) 631
BpmI CTGGAG 2 cut(s) 299, 747
Bpu1102I GCTNAGC 1 cut(s) 395
BpuEI CTTGAG 1 cut(s) 736
BsaI GGTCTC 1 cut(s) 452
BsaWI WCCGGW 3 cut(s) 82, 497, 514
BsaXI ACNNNNNCTCC 2 cut(s) 720, 750
Bsc4I CCNNNNNNNGG 2 cut(s) 304, 725
Bse118I RCCGGY 2 cut(s) 82, 148
Bse1I ACTGG 4 cut(s) 282, 298, 637, 812
Bse3DI GCAATG 1 cut(s) 123
BseAI TCCGGA 1 cut(s) 514
BseGI GGATG 2 cut(s) 170, 679
BseLI CCNNNNNNNGG 2 cut(s) 304, 725
BseMI GCAATG 1 cut(s) 123
BseMII CTCAG 1 cut(s) 686
BseNI ACTGG 4 cut(s) 282, 298, 637, 812
BseRI GAGGAG 2 cut(s) 40, 302
BseXI GCAGC 3 cut(s) 520, 725, 839
BsgI GTGCAG 1 cut(s) 162
Bsh1236I CGCG 2 cut(s) 136, 401
BshFI GGCC 3 cut(s) 387, 455, 531
BshTI ACCGGT 1 cut(s) 82
BsiHKAI GWGCWC 1 cut(s) 312
BsiSI CCGG 5 cut(s) 83, 149, 498, 515, 603
BslFI GGGAC 1 cut(s) 242
BslI CCNNNNNNNGG 2 cut(s) 304, 725
BsmAI GTCTC 3 cut(s) 452, 604, 670
BsmBI CGTCTC 1 cut(s) 604
BsmFI GGGAC 1 cut(s) 242
BsnI GGCC 3 cut(s) 387, 455, 531
Bso31I GGTCTC 1 cut(s) 452
Bsp1286I GDGCHC 2 cut(s) 312, 637
Bsp13I TCCGGA 1 cut(s) 514
Bsp143I GATC 1 cut(s) 358
Bsp1720I GCTNAGC 1 cut(s) 395
BspACI CCGC 6 cut(s) 134, 152, 331, 401, 460, 690
BspANI GGCC 3 cut(s) 387, 455, 531
BspCNI CTCAG 1 cut(s) 685
BspEI TCCGGA 1 cut(s) 514
BspFNI CGCG 2 cut(s) 136, 401
BspLI GGNNCC 2 cut(s) 530, 634
BspMAI CTGCAG 1 cut(s) 510
BspQI GCTCTTC 1 cut(s) 270
BspTNI GGTCTC 1 cut(s) 452
BsrDI GCAATG 1 cut(s) 123
BsrFI RCCGGY 2 cut(s) 82, 148
BsrI ACTGG 4 cut(s) 282, 298, 637, 812
BssAI RCCGGY 2 cut(s) 82, 148
BssMI GATC 1 cut(s) 358
BssNAI GTATAC 1 cut(s) 303
BssSI CACGAG 1 cut(s) 449
Bst1107I GTATAC 1 cut(s) 303
Bst2BI CACGAG 1 cut(s) 449
Bst4CI ACNGT 3 cut(s) 64, 242, 307
Bst6I CTCTTC 4 cut(s) 270, 604, 609, 819
BstC8I GCNNGC 4 cut(s) 150, 457, 464, 690
BstDEI CTNAG 3 cut(s) 54, 395, 672
BstF5I GGATG 2 cut(s) 170, 679
BstFNI CGCG 2 cut(s) 136, 401
BstHHI GCGC 1 cut(s) 401
BstKTI GATC 1 cut(s) 361
BstMAI GTCTC 3 cut(s) 452, 604, 670
BstMBI GATC 1 cut(s) 358
BstMWI GCNNNNNNNGC 3 cut(s) 149, 562, 722
BstSFI CTRYAG 3 cut(s) 241, 345, 506
BstUI CGCG 2 cut(s) 136, 401
BstV1I GCAGC 3 cut(s) 520, 725, 839
BstZ17I GTATAC 1 cut(s) 303
BsuI GTATCC 1 cut(s) 826
BsuRI GGCC 3 cut(s) 387, 455, 531
BtsCI GGATG 2 cut(s) 170, 679
BtsI GCAGTG 2 cut(s) 150, 333
BtsIMutI CAGTG 3 cut(s) 150, 333, 474
Cac8I GCNNGC 4 cut(s) 150, 457, 464, 690
CfoI GCGC 1 cut(s) 401
Cfr10I RCCGGY 2 cut(s) 82, 148
Cfr13I GGNCC 2 cut(s) 154, 529
Csp6I GTAC 1 cut(s) 65
CspAI ACCGGT 1 cut(s) 82
CviAII CATG 4 cut(s) 71, 118, 225, 284
CviQI GTAC 1 cut(s) 65
DdeI CTNAG 3 cut(s) 54, 395, 672
DpnI GATC 1 cut(s) 360
DpnII GATC 1 cut(s) 358
EaeI YGGCCR 1 cut(s) 453
Eam1104I CTCTTC 4 cut(s) 270, 604, 609, 819
EarI CTCTTC 4 cut(s) 270, 604, 609, 819
EciI GGCGGA 1 cut(s) 167
Eco24I GRGCYC 1 cut(s) 637
Eco31I GGTCTC 1 cut(s) 452
Eco47I GGWCC 1 cut(s) 154
EcoO109I RGGNCCY 1 cut(s) 529
EcoRI GAATTC 1 cut(s) 405
EcoT38I GRGCYC 1 cut(s) 637
Esp3I CGTCTC 1 cut(s) 604
FaeI CATG 4 cut(s) 74, 121, 228, 287
FalI AAGNNNNNCTT 2 cut(s) 252, 284
FaqI GGGAC 1 cut(s) 242
FatI CATG 4 cut(s) 70, 117, 224, 283
FauI CCCGC 2 cut(s) 394, 697
FblI GTMKAC 1 cut(s) 302
Fnu4HI GCNGC 5 cut(s) 134, 460, 509, 714, 853
FokI GGATG 2 cut(s) 177, 666
FriOI GRGCYC 1 cut(s) 637
Fsp4HI GCNGC 5 cut(s) 134, 460, 509, 714, 853
FspBI CTAG 1 cut(s) 389
GlaI GCGC 1 cut(s) 400
GluI GCNGC 5 cut(s) 134, 460, 509, 714, 853
GsuI CTGGAG 2 cut(s) 299, 747
HaeIII GGCC 3 cut(s) 387, 455, 531
HapII CCGG 5 cut(s) 83, 149, 498, 515, 603
HhaI GCGC 1 cut(s) 401
Hin1II CATG 4 cut(s) 74, 121, 228, 287
Hin6I GCGC 1 cut(s) 399
HinP1I GCGC 1 cut(s) 399
HincII GTYRAC 1 cut(s) 222
HindII GTYRAC 1 cut(s) 222
HindIII AAGCTT 3 cut(s) 563, 741, 786
HinfI GANTC 4 cut(s) 14, 651, 701, 731
HpaII CCGG 5 cut(s) 83, 149, 498, 515, 603
HphI GGTGA 1 cut(s) 368
Hpy166II GTNNAC 3 cut(s) 222, 275, 303
Hpy188I TCNGA 6 cut(s) 195, 561, 675, 700, 793, 860
Hpy188III TCNNGA 3 cut(s) 203, 369, 515
Hpy8I GTNNAC 3 cut(s) 222, 275, 303
Hpy99I CGWCG 6 cut(s) 101, 550, 590, 593, 596, 599
HpyCH4III ACNGT 3 cut(s) 64, 242, 307
HpyCH4V TGCA 4 cut(s) 143, 338, 508, 855
HpyF10VI GCNNNNNNNGC 3 cut(s) 149, 562, 722
HpyF3I CTNAG 3 cut(s) 54, 395, 672
Hsp92II CATG 4 cut(s) 74, 121, 228, 287
HspAI GCGC 1 cut(s) 399
Kpn2I TCCGGA 1 cut(s) 514
KroI GCCGGC 1 cut(s) 148
KroNI GCCGGC 1 cut(s) 150
Kzo9I GATC 1 cut(s) 358
LguI GCTCTTC 1 cut(s) 270
LmnI GCTCC 5 cut(s) 53, 196, 280, 315, 632
Lsp1109I GCAGC 3 cut(s) 520, 725, 839
LweI GCATC 4 cut(s) 155, 208, 565, 669
MaeI CTAG 1 cut(s) 389
MaeIII GTNAC 2 cut(s) 85, 539
MalI GATC 1 cut(s) 360
MboI GATC 1 cut(s) 358
MboII GAAGA 5 cut(s) 257, 621, 626, 739, 836
MhlI GDGCHC 2 cut(s) 312, 637
MlsI TGGCCA 1 cut(s) 455
MluCI AATT 3 cut(s) 339, 405, 665
MluNI TGGCCA 1 cut(s) 455
MnlI CCTC 8 cut(s) 61, 104, 323, 542, 666, 681, 773, 826
Mox20I TGGCCA 1 cut(s) 455
MroI TCCGGA 1 cut(s) 514
MroNI GCCGGC 1 cut(s) 148
MroXI GAANNNNTTC 1 cut(s) 787
MscI TGGCCA 1 cut(s) 455
MslI CAYNNNNRTG 1 cut(s) 128
Msp20I TGGCCA 1 cut(s) 455
MspA1I CMGCKG 1 cut(s) 692
MspI CCGG 5 cut(s) 83, 149, 498, 515, 603
MvnI CGCG 2 cut(s) 136, 401
MwoI GCNNNNNNNGC 3 cut(s) 149, 562, 722
NaeI GCCGGC 1 cut(s) 150
NdeII GATC 1 cut(s) 358
NgoMIV GCCGGC 1 cut(s) 148
NlaIII CATG 4 cut(s) 74, 121, 228, 287
NlaIV GGNNCC 2 cut(s) 530, 634
NmuCI GTSAC 1 cut(s) 85
PciSI GCTCTTC 1 cut(s) 270
PcsI WCGNNNNNNNCGW 2 cut(s) 591, 594
PdiI GCCGGC 1 cut(s) 150
PdmI GAANNNNTTC 1 cut(s) 787
PfeI GAWTC 4 cut(s) 14, 651, 701, 731
PinAI ACCGGT 1 cut(s) 82
PkrI GCNGC 5 cut(s) 135, 461, 510, 715, 854
PspN4I GGNNCC 2 cut(s) 530, 634
PspPI GGNCC 2 cut(s) 154, 529
PstI CTGCAG 1 cut(s) 510
RsaI GTAC 1 cut(s) 66
RsaNI GTAC 1 cut(s) 65
RseI CAYNNNNRTG 1 cut(s) 128
SapI GCTCTTC 1 cut(s) 270
SatI GCNGC 5 cut(s) 134, 460, 509, 714, 853
Sau3AI GATC 1 cut(s) 358
Sau96I GGNCC 2 cut(s) 154, 529
ScaI AGTACT 1 cut(s) 66
SduI GDGCHC 2 cut(s) 312, 637
SfaNI GCATC 4 cut(s) 155, 208, 565, 669
SfcI CTRYAG 3 cut(s) 241, 345, 506
SinI GGWCC 1 cut(s) 154
SmiMI CAYNNNNRTG 1 cut(s) 128
SmlI CTYRAG 1 cut(s) 751
SmoI CTYRAG 1 cut(s) 751
Sse9I AATT 3 cut(s) 339, 405, 665
SsiI CCGC 6 cut(s) 134, 152, 331, 401, 460, 690
SspI AATATT 1 cut(s) 36
SspMI CTAG 1 cut(s) 389
TaaI ACNGT 3 cut(s) 64, 242, 307
TaqI TCGA 3 cut(s) 96, 216, 545
TasI AATT 3 cut(s) 339, 405, 665
TatI WGTACW 1 cut(s) 64
TauI GCSGC 2 cut(s) 136, 462
TfiI GAWTC 4 cut(s) 14, 651, 701, 731
TscAI CASTG 3 cut(s) 150, 340, 481
TseFI GTSAC 1 cut(s) 85
TseI GCWGC 3 cut(s) 508, 713, 852
Tsp45I GTSAC 1 cut(s) 85
TspDTI ATGAA 2 cut(s) 412, 659
TspGWI ACGGA 3 cut(s) 177, 339, 563
TspRI CASTG 3 cut(s) 150, 340, 481
VpaK11BI GGWCC 1 cut(s) 154
XapI RAATTY 2 cut(s) 405, 665
XmiI GTMKAC 1 cut(s) 302
XmnI GAANNNNTTC 1 cut(s) 787
XspI CTAG 1 cut(s) 389
ZrmI AGTACT 1 cut(s) 66
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.