Rmu_sc0004536.1_g000013

Nucleolar protein 6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004536.1
OG0010610
Physical Location & Seq
Forward (+)
49979 .. 57676
7698 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004536.1_g000013.1.cds

Sequence Viewer

Length: 1242 bp
atgatcatatatgccttaaagactttgttacttcagtttggtgaagatataattcccacagcaccatctctttttaggattccaaaattgaacctgcagcggaataacgttcgtgctgtgagtaaagggggcattcctcagtggactcccaagtacaatagtagtattttggaagacatgtttatagaagatacagaacaacttgtcaagcaaacttttcttgggtcaaaggaattacgagaaggtttagttttgctgaaggtctgggctcgccgcaggataccaatttatgcttatgattgcctgaatggttttttaatctctgtcatattggcatatcttgtggataggaaccatatcaataaatcaatgaaggcaatgcaaatatttcgtgttacaatgaaattcattgctacttcagatttgtggaaacatgggatctactttactccaaaaggccaaaaagctatttcaaaagaggtctcctttttactcgatttggccatcgagacgaccaagtacatcttccccaagcagtttgaggctcagactctggaggaagctttcatgttagtgtggcacgggagcattgtggtcactgtaggcgagagggttcctcttgtggaggtggtggaggagtttctcgtggtagtgtcggcgcttctcgtgaaggtggcgagggcacttctcacggacgtggctagggtgcttcttgccaaggtgccgttggcacttcccaaggaggtggagagggtgttggagattcttcactcgccacgagggcggatgctccttagtgagacccccattgatcaaccagttctgagtatgacgaaggagcagattgccaaaatgaggacgacctggggcattccgggcaatgtattgttacgctccttgagggatggtgaatgttttagccaccccaaggtggggtgggcgtgcttttatgagtatcagcttaagtgtgggctgacattcccgataccggaggagctgcagtatctgttatcgtgcctaaatgtatcgttggggcagctatcgccaaacacactaaggcaattgatgggagtattgctattgtggaagctcagtagactgctatgtcccaacattggcgagtttaactccttgttcaagatgcaatactcgatgaaggctggcaatagcgggtgtgctaattttagggtgcgtggcagcgcgattgtcgaggacatttcgtcaaacatttatgcttgctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
Nrap Nrap_D2 Nrap_D3 Nrap_D4 Nrap_D5 Nrap_D6

Protein Analysis

413

Amino Acids

46.82

Weight (kDa)

9.19

Isoelectric Point (pI)

43.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1104
Acc36I ACCTGC 1 cut(s) 102
AccB1I GGYRCC 1 cut(s) 721
AccI GTMKAC 1 cut(s) 1096
AccII CGCG 1 cut(s) 1202
AciI CCGC 4 cut(s) 100, 274, 784, 1170
AclI AACGTT 1 cut(s) 108
AclWI GGATC 1 cut(s) 446
AcoI YGGCCR 1 cut(s) 501
AcsI RAATTY 1 cut(s) 404
AcuI CTGAAG 3 cut(s) 17, 278, 402
AfaI GTAC 2 cut(s) 155, 521
AfiI CCNNNNNNNGG 7 cut(s) 855, 928, 931, 932, 933, 988, 1115
AflII CTTAAG 1 cut(s) 962
AflIII ACRYGT 1 cut(s) 177
AgsI TTSAA 3 cut(s) 91, 474, 1138
AhdI GACNNNNNGTC 1 cut(s) 1219
AjiI CACGTC 1 cut(s) 697
AjnI CCWGG 1 cut(s) 863
AjuI GAANNNNNNNTTGG 2 cut(s) 509, 541
AleI CACNNNNGTG 1 cut(s) 695
AluBI AGCT 6 cut(s) 467, 563, 961, 997, 1039, 1090
AluI AGCT 6 cut(s) 467, 563, 961, 997, 1039, 1090
Alw26I GTCTC 3 cut(s) 487, 503, 794
AlwI GGATC 1 cut(s) 446
AlwNI CAGNNNCTG 2 cut(s) 553, 1006
AoxI GGCC 2 cut(s) 457, 501
ApeKI GCWGC 4 cut(s) 97, 997, 1036, 1197
ApoI RAATTY 1 cut(s) 404
AspLEI GCGC 2 cut(s) 661, 1202
AsuC2I CCSGG 1 cut(s) 876
AsuHPI GGTGA 2 cut(s) 53, 920
BaeGI GKGCMC 1 cut(s) 685
BalI TGGCCA 1 cut(s) 503
BanI GGYRCC 1 cut(s) 721
BanII GRGCYC 1 cut(s) 271
BauI CACGAG 3 cut(s) 644, 665, 777
BbsI GAAGAC 1 cut(s) 180
BbvI GCAGC 4 cut(s) 109, 984, 1048, 1209
BccI CCATC 4 cut(s) 73, 512, 899, 1060
BceAI ACGGC 1 cut(s) 709
BcgI CGANNNNNNTGC 2 cut(s) 371, 405
BciT130I CCWGG 1 cut(s) 865
BciVI GTATCC 1 cut(s) 273
BclI TGATCA 2 cut(s) 3, 811
BcnI CCSGG 1 cut(s) 876
BcoDI GTCTC 3 cut(s) 487, 503, 794
BfaI CTAG 1 cut(s) 702
BfmI CTRYAG 3 cut(s) 95, 600, 998
BfoI RGCGCY 1 cut(s) 662
BfrI CTTAAG 1 cut(s) 962
BfuAI ACCTGC 1 cut(s) 102
BfuI GTATCC 1 cut(s) 273
BglI GCCNNNNNGGC 1 cut(s) 781
BisI GCNGC 5 cut(s) 98, 274, 998, 1037, 1198
BlsI GCNGC 5 cut(s) 99, 275, 999, 1038, 1199
Bme1390I CCNGG 2 cut(s) 865, 876
BmeRI GACNNNNNGTC 1 cut(s) 1219
BmgBI CACGTC 1 cut(s) 697
BmiI GGNNCC 3 cut(s) 353, 615, 723
BmrFI CCNGG 2 cut(s) 865, 876
BmsI GCATC 2 cut(s) 777, 1131
BpiI GAAGAC 1 cut(s) 180
BplI GAGNNNNNCTC 4 cut(s) 601, 633, 1112, 1144
BpmI CTGGAG 1 cut(s) 575
BpuEI CTTGAG 1 cut(s) 919
BpuMI CCSGG 1 cut(s) 876
BsaI GGTCTC 2 cut(s) 487, 794
BsaJI CCNNGG 4 cut(s) 717, 738, 864, 927
BsaWI WCCGGW 1 cut(s) 988
BsaXI ACNNNNNCTCC 4 cut(s) 740, 770, 986, 1016
Bsc4I CCNNNNNNNGG 7 cut(s) 855, 928, 931, 932, 933, 988, 1115
Bse1I ACTGG 1 cut(s) 818
Bse3DI GCAATG 3 cut(s) 384, 408, 886
BseBI CCWGG 1 cut(s) 865
BseDI CCNNGG 4 cut(s) 717, 738, 864, 927
BseGI GGATG 2 cut(s) 792, 910
BseLI CCNNNNNNNGG 7 cut(s) 855, 928, 931, 932, 933, 988, 1115
BseMI GCAATG 3 cut(s) 384, 408, 886
BseMII CTCAG 4 cut(s) 152, 560, 815, 1105
BseNI ACTGG 1 cut(s) 818
BseRI GAGGAG 2 cut(s) 650, 1007
BseSI GKGCMC 1 cut(s) 685
BseXI GCAGC 4 cut(s) 109, 984, 1048, 1209
Bsh1236I CGCG 1 cut(s) 1202
BshFI GGCC 2 cut(s) 459, 503
BshNI GGYRCC 1 cut(s) 721
BsiSI CCGG 2 cut(s) 875, 989
BslFI GGGAC 1 cut(s) 1092
BslI CCNNNNNNNGG 7 cut(s) 855, 928, 931, 932, 933, 988, 1115
BsmAI GTCTC 3 cut(s) 487, 503, 794
BsmBI CGTCTC 1 cut(s) 503
BsmFI GGGAC 1 cut(s) 1092
BsmI GAATGC 2 cut(s) 132, 870
BsnI GGCC 2 cut(s) 459, 503
Bso31I GGTCTC 2 cut(s) 487, 794
Bsp1286I GDGCHC 2 cut(s) 271, 685
Bsp143I GATC 3 cut(s) 3, 438, 811
BspACI CCGC 4 cut(s) 100, 274, 784, 1170
BspANI GGCC 2 cut(s) 459, 503
BspCNI CTCAG 4 cut(s) 151, 559, 816, 1104
BspFNI CGCG 1 cut(s) 1202
BspLI GGNNCC 3 cut(s) 353, 615, 723
BspMAI CTGCAG 2 cut(s) 99, 1002
BspMI ACCTGC 1 cut(s) 102
BspPI GGATC 1 cut(s) 446
BspT107I GGYRCC 1 cut(s) 721
BspTI CTTAAG 1 cut(s) 962
BspTNI GGTCTC 2 cut(s) 487, 794
BsrDI GCAATG 3 cut(s) 384, 408, 886
BsrI ACTGG 1 cut(s) 818
BssECI CCNNGG 4 cut(s) 717, 738, 864, 927
BssMI GATC 3 cut(s) 3, 438, 811
BssSI CACGAG 3 cut(s) 644, 665, 777
BssT1I CCWWGG 3 cut(s) 717, 738, 927
Bst2BI CACGAG 3 cut(s) 644, 665, 777
Bst2UI CCWGG 1 cut(s) 865
Bst4CI ACNGT 1 cut(s) 601
BstAFI CTTAAG 1 cut(s) 962
BstC8I GCNNGC 4 cut(s) 271, 943, 1162, 1237
BstDEI CTNAG 6 cut(s) 138, 546, 794, 824, 1055, 1091
BstF5I GGATG 2 cut(s) 792, 910
BstFNI CGCG 1 cut(s) 1202
BstH2I RGCGCY 1 cut(s) 662
BstHHI GCGC 2 cut(s) 661, 1202
BstKTI GATC 3 cut(s) 6, 441, 814
BstMAI GTCTC 3 cut(s) 487, 503, 794
BstMBI GATC 3 cut(s) 3, 438, 811
BstMWI GCNNNNNNNGC 3 cut(s) 781, 876, 1042
BstNI CCWGG 1 cut(s) 865
BstNSI RCATGY 1 cut(s) 181
BstSCI CCNGG 2 cut(s) 863, 874
BstSFI CTRYAG 3 cut(s) 95, 600, 998
BstSLI GKGCMC 1 cut(s) 685
BstUI CGCG 1 cut(s) 1202
BstV1I GCAGC 4 cut(s) 109, 984, 1048, 1209
BstV2I GAAGAC 1 cut(s) 180
BstX2I RGATCY 1 cut(s) 438
BstXI CCANNNNNNTGG 1 cut(s) 745
BstYI RGATCY 1 cut(s) 438
BsuI GTATCC 1 cut(s) 273
BsuRI GGCC 2 cut(s) 459, 503
BtrI CACGTC 1 cut(s) 697
BtsCI GGATG 2 cut(s) 792, 910
BtsIMutI CAGTG 2 cut(s) 146, 597
BveI ACCTGC 1 cut(s) 102
Cac8I GCNNGC 4 cut(s) 271, 943, 1162, 1237
CaiI CAGNNNCTG 2 cut(s) 553, 1006
CfoI GCGC 2 cut(s) 661, 1202
Csp6I GTAC 2 cut(s) 154, 520
CviAII CATG 3 cut(s) 178, 434, 568
CviQI GTAC 2 cut(s) 154, 520
DdeI CTNAG 6 cut(s) 138, 546, 794, 824, 1055, 1091
DpnI GATC 3 cut(s) 5, 440, 813
DpnII GATC 3 cut(s) 3, 438, 811
DrdI GACNNNNNNGTC 1 cut(s) 1104
DriI GACNNNNNGTC 1 cut(s) 1219
DseDI GACNNNNNNGTC 1 cut(s) 1104
EaeI YGGCCR 1 cut(s) 501
Eam1105I GACNNNNNGTC 1 cut(s) 1219
EciI GGCGGA 1 cut(s) 799
Eco130I CCWWGG 3 cut(s) 717, 738, 927
Eco24I GRGCYC 1 cut(s) 271
Eco31I GGTCTC 2 cut(s) 487, 794
Eco57I CTGAAG 3 cut(s) 17, 278, 402
EcoRII CCWGG 1 cut(s) 863
EcoT14I CCWWGG 3 cut(s) 717, 738, 927
EcoT38I GRGCYC 1 cut(s) 271
ErhI CCWWGG 3 cut(s) 717, 738, 927
Esp3I CGTCTC 1 cut(s) 503
FaeI CATG 3 cut(s) 181, 437, 571
FalI AAGNNNNNCTT 2 cut(s) 509, 541
FaqI GGGAC 1 cut(s) 1092
FatI CATG 3 cut(s) 177, 433, 567
FauI CCCGC 1 cut(s) 1163
FbaI TGATCA 2 cut(s) 3, 811
FblI GTMKAC 1 cut(s) 1096
Fnu4HI GCNGC 5 cut(s) 98, 274, 998, 1037, 1198
FokI GGATG 2 cut(s) 799, 917
FriOI GRGCYC 1 cut(s) 271
Fsp4HI GCNGC 5 cut(s) 98, 274, 998, 1037, 1198
FspBI CTAG 1 cut(s) 702
GlaI GCGC 2 cut(s) 660, 1201
GluI GCNGC 5 cut(s) 98, 274, 998, 1037, 1198
GsuI CTGGAG 1 cut(s) 575
HaeII RGCGCY 1 cut(s) 662
HaeIII GGCC 2 cut(s) 459, 503
HapII CCGG 2 cut(s) 875, 989
HhaI GCGC 2 cut(s) 661, 1202
Hin1II CATG 3 cut(s) 181, 437, 571
Hin6I GCGC 2 cut(s) 659, 1200
HinP1I GCGC 2 cut(s) 659, 1200
HindIII AAGCTT 1 cut(s) 561
HinfI GANTC 4 cut(s) 79, 145, 550, 763
HpaII CCGG 2 cut(s) 875, 989
HphI GGTGA 2 cut(s) 53, 920
Hpy166II GTNNAC 2 cut(s) 144, 1097
Hpy188I TCNGA 3 cut(s) 421, 549, 825
Hpy188III TCNNGA 5 cut(s) 508, 554, 667, 982, 1138
Hpy8I GTNNAC 2 cut(s) 144, 1097
HpyAV CCTTC 6 cut(s) 236, 253, 367, 664, 829, 1150
HpyCH4III ACNGT 1 cut(s) 601
HpyCH4IV ACGT 2 cut(s) 108, 696
HpyCH4V TGCA 4 cut(s) 97, 382, 1000, 1144
HpyF10VI GCNNNNNNNGC 3 cut(s) 781, 876, 1042
HpyF3I CTNAG 6 cut(s) 138, 546, 794, 824, 1055, 1091
HpySE526I ACGT 2 cut(s) 108, 696
Hsp92II CATG 3 cut(s) 181, 437, 571
HspAI GCGC 2 cut(s) 659, 1200
Ksp22I TGATCA 2 cut(s) 3, 811
Kzo9I GATC 3 cut(s) 3, 438, 811
LmnI GCTCC 5 cut(s) 585, 795, 838, 899, 994
Lsp1109I GCAGC 4 cut(s) 109, 984, 1048, 1209
LweI GCATC 2 cut(s) 777, 1131
MaeI CTAG 1 cut(s) 702
MaeII ACGT 2 cut(s) 108, 696
MaeIII GTNAC 4 cut(s) 27, 394, 595, 888
MalI GATC 3 cut(s) 5, 440, 813
MboI GATC 3 cut(s) 3, 438, 811
MboII GAAGA 5 cut(s) 56, 185, 200, 517, 758
MfeI CAATTG 1 cut(s) 1061
MflI RGATCY 1 cut(s) 438
MhlI GDGCHC 2 cut(s) 271, 685
MlsI TGGCCA 1 cut(s) 503
MluCI AATT 7 cut(s) 51, 86, 233, 285, 404, 1061, 1180
MluNI TGGCCA 1 cut(s) 503
MlyI GAGTC 2 cut(s) 139, 544
MmeI TCCRAC 1 cut(s) 738
Mox20I TGGCCA 1 cut(s) 503
MscI TGGCCA 1 cut(s) 503
MseI TTAA 4 cut(s) 17, 317, 963, 1125
MslI CAYNNNNRTG 2 cut(s) 572, 695
Msp20I TGGCCA 1 cut(s) 503
MspA1I CMGCKG 1 cut(s) 100
MspCI CTTAAG 1 cut(s) 962
MspI CCGG 2 cut(s) 875, 989
MspR9I CCNGG 2 cut(s) 865, 876
MunI CAATTG 1 cut(s) 1061
Mva1269I GAATGC 2 cut(s) 132, 870
MvaI CCWGG 1 cut(s) 865
MvnI CGCG 1 cut(s) 1202
MwoI GCNNNNNNNGC 3 cut(s) 781, 876, 1042
NciI CCSGG 1 cut(s) 876
NdeII GATC 3 cut(s) 3, 438, 811
NlaIII CATG 3 cut(s) 181, 437, 571
NlaIV GGNNCC 3 cut(s) 353, 615, 723
NmuCI GTSAC 1 cut(s) 595
NspI RCATGY 1 cut(s) 181
OliI CACNNNNGTG 1 cut(s) 695
PciI ACATGT 1 cut(s) 177
PctI GAATGC 2 cut(s) 132, 870
PfeI GAWTC 2 cut(s) 79, 763
PkrI GCNGC 5 cut(s) 99, 275, 999, 1038, 1199
PleI GAGTC 2 cut(s) 139, 544
PpsI GAGTC 2 cut(s) 139, 544
PscI ACATGT 1 cut(s) 177
Psp1406I AACGTT 1 cut(s) 108
Psp6I CCWGG 1 cut(s) 863
PspGI CCWGG 1 cut(s) 863
PspN4I GGNNCC 3 cut(s) 353, 615, 723
PstI CTGCAG 2 cut(s) 99, 1002
PstNI CAGNNNCTG 2 cut(s) 553, 1006
PsuI RGATCY 1 cut(s) 438
RsaI GTAC 2 cut(s) 155, 521
RsaNI GTAC 2 cut(s) 154, 520
RseI CAYNNNNRTG 2 cut(s) 572, 695
SaqAI TTAA 4 cut(s) 17, 317, 963, 1125
SatI GCNGC 5 cut(s) 98, 274, 998, 1037, 1198
Sau3AI GATC 3 cut(s) 3, 438, 811
SchI GAGTC 2 cut(s) 139, 544
ScrFI CCNGG 2 cut(s) 865, 876
SduI GDGCHC 2 cut(s) 271, 685
SfaNI GCATC 2 cut(s) 777, 1131
SfcI CTRYAG 3 cut(s) 95, 600, 998
SmiMI CAYNNNNRTG 2 cut(s) 572, 695
SmlI CTYRAG 2 cut(s) 898, 962
SmoI CTYRAG 2 cut(s) 898, 962
Sse9I AATT 7 cut(s) 51, 86, 233, 285, 404, 1061, 1180
SsiI CCGC 4 cut(s) 100, 274, 784, 1170
SspI AATATT 1 cut(s) 387
SspMI CTAG 1 cut(s) 702
StyD4I CCNGG 2 cut(s) 863, 874
StyI CCWWGG 3 cut(s) 717, 738, 927
TaaI ACNGT 1 cut(s) 601
TaiI ACGT 2 cut(s) 111, 699
TaqI TCGA 4 cut(s) 495, 507, 1151, 1209
TasI AATT 7 cut(s) 51, 86, 233, 285, 404, 1061, 1180
TatI WGTACW 2 cut(s) 153, 519
TauI GCSGC 1 cut(s) 276
TfiI GAWTC 2 cut(s) 79, 763
Tru1I TTAA 4 cut(s) 17, 317, 963, 1125
Tru9I TTAA 4 cut(s) 17, 317, 963, 1125
TscAI CASTG 2 cut(s) 146, 604
TseFI GTSAC 1 cut(s) 595
TseI GCWGC 4 cut(s) 97, 997, 1036, 1197
Tsp45I GTSAC 1 cut(s) 595
TspDTI ATGAA 5 cut(s) 386, 397, 416, 556, 1169
TspGWI ACGGA 1 cut(s) 707
TspRI CASTG 2 cut(s) 146, 604
Vha464I CTTAAG 1 cut(s) 962
XapI RAATTY 1 cut(s) 404
XceI RCATGY 1 cut(s) 181
XcmI CCANNNNNNNNNTGG 1 cut(s) 724
XmiI GTMKAC 1 cut(s) 1096
XspI CTAG 1 cut(s) 702
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.