Rroxscaffold_2G00147870

phosphopyruvate hydratase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0010610
Physical Location & Seq
Reverse (-)
85588781 .. 85591713
2933 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00147870.1

Sequence Viewer

Length: 1041 bp
ATGTCATTTCGAGCTTGGATCCTGGGGATCCTAACAAGCTGTATCGAGTACGCACTTGGGCCTTTTCCCGGTGAGTCCGGCGGTGGGATCTCCGTCGGGTGTTACAACACTCATTCTTATCACCCTCACGGTAGCCCTAGCTACCCATCCCTTCCTTCGCAATATTCATCGCAACCTAGCTACCCACCACCTCCTTCTCAATTTCCATCACAACCAAGCTACCCACCACTACCACGTTCCCAAACAATCATTAATGGGAAATGGAGTCGGCGAGAGGCATGGAAAAGACAGCTAGAGCTTAGAGCTTTAACTCACTTCCTTAATAAGTCTATCAAGGTAAACTCGGGATCATTCCCTTATGTGGAGAATGTGAATGACATCATTGGACCAGCCTTGATTGCAATGAACCCAATGGAACAAACCCACATTGACAATTATATGGTTCAACAACTTAAGGGAATTACAAATGAATGGAGTTGGTGCAAACAAGAGCTCGGAGAAAATGGTATAGTAGCGATGTTTGTTGTTGTTTATAAAGCAAGTGCCATCGTAAACAAGATACCCCTCTTCAAGCGCATAGCCAATCTTCTGGAAAAAAGACCTTGGTTCCGCTTTGTTCCAACTAAACCCGGTAGTGAGTATAATGATATTCTCAATGTTTCGGTGGTCTCGAGAGGAGCTCGGCCCGGTGAACACGGGATGTATGGGTCCGCTACTATCTCTATAGGTCTCGCTTATCGCAAAACGAGACAAAGGTCGGAGGGAAGACCGGCGAGGCGACGGCGCATAACATCCGTAACACCCGGGGATGTCGAACACGTGGTGAGATGCGGGGTTGTCGTCCTTGCACGTCCAATGGTCCCGGTGTTCTTGAGGTCCTTATATAAGGACGAGAGGAGAGATGTGGAGATAGTTACCGTGCTGCGTTTGTGTTGGAGCTTGAGAGAAGAACTGGGTGAGGTTGGGGTCGTAGTTGTGTGGAGAGCTTTTGACCGGGCGTGGAGTAGTCGTCTTCGTCTCGTATTTCGATCGGAGGAGTGA

Protein Analysis

346

Amino Acids

39.19

Weight (kDa)

9.8

Isoelectric Point (pI)

61.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Enolase_N PF03952 120 - 192 9.7e-09 Enolase, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 534
AciI CCGC 4 cut(s) 81, 610, 711, 831
AclWI GGATC 6 cut(s) 13, 22, 26, 35, 95, 355
AcvI CACGTG 1 cut(s) 820
AdeI CACNNNGTG 1 cut(s) 823
AfaI GTAC 1 cut(s) 50
AfiI CCNNNNNNNGG 3 cut(s) 68, 84, 361
AflII CTTAAG 1 cut(s) 452
AflIII ACRYGT 1 cut(s) 817
AgsI TTSAA 2 cut(s) 446, 571
AjiI CACGTC 1 cut(s) 851
AjnI CCWGG 1 cut(s) 21
Alw21I GWGCWC 2 cut(s) 495, 682
Alw26I GTCTC 4 cut(s) 673, 734, 742, 1022
AlwI GGATC 6 cut(s) 13, 22, 26, 35, 95, 355
Ama87I CYCGRG 3 cut(s) 343, 670, 803
AoxI GGCC 2 cut(s) 59, 683
ApeKI GCWGC 1 cut(s) 922
AseI ATTAAT 1 cut(s) 252
AspLEI GCGC 2 cut(s) 576, 786
AspS9I GGNCC 6 cut(s) 59, 386, 684, 708, 859, 876
AsuC2I CCSGG 7 cut(s) 69, 630, 687, 804, 805, 863, 995
AsuHPI GGTGA 5 cut(s) 83, 113, 701, 835, 968
AvaI CYCGRG 3 cut(s) 343, 670, 803
AvaII GGWCC 4 cut(s) 386, 708, 859, 876
BamHI GGATCC 2 cut(s) 18, 27
BanII GRGCYC 2 cut(s) 495, 682
BbrPI CACGTG 1 cut(s) 820
BbsI GAAGAC 2 cut(s) 772, 1004
Bbv12I GWGCWC 2 cut(s) 495, 682
BbvI GCAGC 1 cut(s) 909
BccI CCATC 3 cut(s) 154, 214, 554
BceAI ACGGC 1 cut(s) 797
BciT130I CCWGG 1 cut(s) 23
BcnI CCSGG 7 cut(s) 69, 630, 687, 804, 805, 863, 995
BcoDI GTCTC 4 cut(s) 673, 734, 742, 1022
BfaI CTAG 3 cut(s) 138, 177, 293
BfmI CTRYAG 1 cut(s) 723
BfrI CTTAAG 1 cut(s) 452
BisI GCNGC 1 cut(s) 923
BlsI GCNGC 1 cut(s) 924
Bme1390I CCNGG 8 cut(s) 23, 69, 630, 687, 804, 805, 863, 995
Bme18I GGWCC 4 cut(s) 386, 708, 859, 876
BmeT110I CYCGRG 3 cut(s) 343, 670, 803
BmgBI CACGTC 1 cut(s) 851
BmgT120I GGNCC 6 cut(s) 59, 386, 684, 708, 859, 876
BmiI GGNNCC 5 cut(s) 20, 29, 608, 709, 861
BmrFI CCNGG 8 cut(s) 23, 69, 630, 687, 804, 805, 863, 995
BmrI ACTGGG 1 cut(s) 962
BmsI GCATC 1 cut(s) 818
BmuI ACTGGG 1 cut(s) 962
BoxI GACNNNNGTC 1 cut(s) 754
BpiI GAAGAC 2 cut(s) 772, 1004
BplI GAGNNNNNCTC 2 cut(s) 664, 696
BpuEI CTTGAG 2 cut(s) 892, 961
BpuMI CCSGG 7 cut(s) 69, 630, 687, 804, 805, 863, 995
BsaAI YACGTR 1 cut(s) 820
BsaI GGTCTC 2 cut(s) 673, 734
BsaJI CCNNGG 4 cut(s) 22, 602, 803, 804
BsaXI ACNNNNNCTCC 2 cut(s) 994, 1024
Bsc4I CCNNNNNNNGG 3 cut(s) 68, 84, 361
Bse118I RCCGGY 1 cut(s) 769
Bse1I ACTGG 1 cut(s) 957
Bse3DI GCAATG 1 cut(s) 408
BseBI CCWGG 1 cut(s) 23
BseDI CCNNGG 4 cut(s) 22, 602, 803, 804
BseGI GGATG 4 cut(s) 146, 705, 791, 814
BseLI CCNNNNNNNGG 3 cut(s) 68, 84, 361
BseMI GCAATG 1 cut(s) 408
BseNI ACTGG 1 cut(s) 957
BseRI GAGGAG 2 cut(s) 690, 910
BseXI GCAGC 1 cut(s) 909
Bsh1285I CGRYCG 1 cut(s) 1031
BshFI GGCC 2 cut(s) 61, 685
BsiEI CGRYCG 1 cut(s) 1031
BsiHKAI GWGCWC 2 cut(s) 495, 682
BsiHKCI CYCGRG 3 cut(s) 343, 670, 803
BsiSI CCGG 8 cut(s) 69, 78, 630, 687, 770, 804, 863, 994
BslFI GGGAC 1 cut(s) 845
BslI CCNNNNNNNGG 3 cut(s) 68, 84, 361
BsmAI GTCTC 4 cut(s) 673, 734, 742, 1022
BsmBI CGTCTC 1 cut(s) 1022
BsmFI GGGAC 1 cut(s) 845
BsnI GGCC 2 cut(s) 61, 685
Bso31I GGTCTC 2 cut(s) 673, 734
BsoBI CYCGRG 3 cut(s) 343, 670, 803
Bsp1286I GDGCHC 2 cut(s) 495, 682
Bsp143I GATC 5 cut(s) 18, 27, 87, 347, 1028
BspACI CCGC 4 cut(s) 81, 610, 711, 831
BspANI GGCC 2 cut(s) 61, 685
BspLI GGNNCC 5 cut(s) 20, 29, 608, 709, 861
BspPI GGATC 6 cut(s) 13, 22, 26, 35, 95, 355
BspTI CTTAAG 1 cut(s) 452
BspTNI GGTCTC 2 cut(s) 673, 734
BsrDI GCAATG 1 cut(s) 408
BsrFI RCCGGY 1 cut(s) 769
BsrI ACTGG 1 cut(s) 957
BssAI RCCGGY 1 cut(s) 769
BssECI CCNNGG 4 cut(s) 22, 602, 803, 804
BssMI GATC 5 cut(s) 18, 27, 87, 347, 1028
BssT1I CCWWGG 1 cut(s) 602
Bst2UI CCWGG 1 cut(s) 23
Bst4CI ACNGT 2 cut(s) 131, 919
Bst6I CTCTTC 1 cut(s) 572
BstAFI CTTAAG 1 cut(s) 452
BstBAI YACGTR 1 cut(s) 820
BstDEI CTNAG 1 cut(s) 299
BstF5I GGATG 4 cut(s) 146, 705, 791, 814
BstHHI GCGC 2 cut(s) 576, 786
BstKTI GATC 5 cut(s) 21, 30, 90, 350, 1031
BstMAI GTCTC 4 cut(s) 673, 734, 742, 1022
BstMBI GATC 5 cut(s) 18, 27, 87, 347, 1028
BstMCI CGRYCG 1 cut(s) 1031
BstMWI GCNNNNNNNGC 1 cut(s) 398
BstNI CCWGG 1 cut(s) 23
BstPAI GACNNNNGTC 1 cut(s) 754
BstSCI CCNGG 8 cut(s) 21, 67, 628, 685, 802, 803, 861, 993
BstSFI CTRYAG 1 cut(s) 723
BstV1I GCAGC 1 cut(s) 909
BstV2I GAAGAC 2 cut(s) 772, 1004
BstX2I RGATCY 3 cut(s) 18, 27, 87
BstXI CCANNNNNNTGG 1 cut(s) 589
BstYI RGATCY 3 cut(s) 18, 27, 87
BsuRI GGCC 2 cut(s) 61, 685
BtgZI GCGATG 2 cut(s) 153, 530
BtrI CACGTC 1 cut(s) 851
BtsCI GGATG 4 cut(s) 146, 705, 791, 814
CfoI GCGC 2 cut(s) 576, 786
Cfr10I RCCGGY 1 cut(s) 769
Cfr13I GGNCC 6 cut(s) 59, 386, 684, 708, 859, 876
Cfr9I CCCGGG 1 cut(s) 803
Csp6I GTAC 1 cut(s) 49
CviAII CATG 1 cut(s) 279
CviQI GTAC 1 cut(s) 49
DdeI CTNAG 1 cut(s) 299
DpnI GATC 5 cut(s) 20, 29, 89, 349, 1030
DpnII GATC 5 cut(s) 18, 27, 87, 347, 1028
DraIII CACNNNGTG 1 cut(s) 823
Eam1104I CTCTTC 1 cut(s) 572
EarI CTCTTC 1 cut(s) 572
Ecl136II GAGCTC 2 cut(s) 493, 680
Eco130I CCWWGG 1 cut(s) 602
Eco24I GRGCYC 2 cut(s) 495, 682
Eco31I GGTCTC 2 cut(s) 673, 734
Eco47I GGWCC 4 cut(s) 386, 708, 859, 876
Eco53kI GAGCTC 2 cut(s) 493, 680
Eco72I CACGTG 1 cut(s) 820
Eco88I CYCGRG 3 cut(s) 343, 670, 803
EcoICRI GAGCTC 2 cut(s) 493, 680
EcoO109I RGGNCCY 1 cut(s) 876
EcoRII CCWGG 1 cut(s) 21
EcoT14I CCWWGG 1 cut(s) 602
EcoT38I GRGCYC 2 cut(s) 495, 682
ErhI CCWWGG 1 cut(s) 602
Esp3I CGTCTC 1 cut(s) 1022
FaeI CATG 1 cut(s) 282
FaqI GGGAC 1 cut(s) 845
FatI CATG 1 cut(s) 278
FauI CCCGC 1 cut(s) 824
Fnu4HI GCNGC 1 cut(s) 923
FokI GGATG 4 cut(s) 133, 712, 778, 821
FriOI GRGCYC 2 cut(s) 495, 682
Fsp4HI GCNGC 1 cut(s) 923
FspBI CTAG 3 cut(s) 138, 177, 293
GlaI GCGC 2 cut(s) 575, 785
GluI GCNGC 1 cut(s) 923
HaeIII GGCC 2 cut(s) 61, 685
HapII CCGG 8 cut(s) 69, 78, 630, 687, 770, 804, 863, 994
HhaI GCGC 2 cut(s) 576, 786
Hin1II CATG 1 cut(s) 282
Hin6I GCGC 2 cut(s) 574, 784
HinP1I GCGC 2 cut(s) 574, 784
HinfI GANTC 2 cut(s) 74, 265
HpaII CCGG 8 cut(s) 69, 78, 630, 687, 770, 804, 863, 994
HphI GGTGA 5 cut(s) 83, 113, 701, 835, 968
Hpy166II GTNNAC 3 cut(s) 340, 553, 692
Hpy188I TCNGA 3 cut(s) 497, 760, 1033
Hpy188III TCNNGA 5 cut(s) 345, 590, 670, 672, 871
Hpy8I GTNNAC 3 cut(s) 340, 553, 692
Hpy99I CGWCG 2 cut(s) 98, 783
HpyAV CCTTC 3 cut(s) 161, 165, 204
HpyCH4III ACNGT 2 cut(s) 131, 919
HpyCH4IV ACGT 3 cut(s) 235, 819, 850
HpyCH4V TGCA 3 cut(s) 401, 483, 848
HpyF10VI GCNNNNNNNGC 1 cut(s) 398
HpyF3I CTNAG 1 cut(s) 299
HpySE526I ACGT 3 cut(s) 235, 819, 850
Hsp92II CATG 1 cut(s) 282
HspAI GCGC 2 cut(s) 574, 784
Kzo9I GATC 5 cut(s) 18, 27, 87, 347, 1028
LmnI GCTCC 2 cut(s) 677, 936
Lsp1109I GCAGC 1 cut(s) 909
LweI GCATC 1 cut(s) 818
MaeI CTAG 3 cut(s) 138, 177, 293
MaeII ACGT 3 cut(s) 235, 819, 850
MaeIII GTNAC 3 cut(s) 101, 796, 913
MalI GATC 5 cut(s) 20, 29, 89, 349, 1030
MboI GATC 5 cut(s) 18, 27, 87, 347, 1028
MboII GAAGA 5 cut(s) 559, 578, 777, 959, 1004
MflI RGATCY 3 cut(s) 18, 27, 87
MhlI GDGCHC 2 cut(s) 495, 682
MluCI AATT 3 cut(s) 200, 433, 459
MlyI GAGTC 2 cut(s) 83, 274
MmeI TCCRAC 3 cut(s) 644, 738, 914
MseI TTAA 4 cut(s) 252, 308, 321, 453
MspCI CTTAAG 1 cut(s) 452
MspI CCGG 8 cut(s) 69, 78, 630, 687, 770, 804, 863, 994
MspR9I CCNGG 8 cut(s) 23, 69, 630, 687, 804, 805, 863, 995
MvaI CCWGG 1 cut(s) 23
MwoI GCNNNNNNNGC 1 cut(s) 398
NciI CCSGG 7 cut(s) 69, 630, 687, 804, 805, 863, 995
NdeII GATC 5 cut(s) 18, 27, 87, 347, 1028
NlaIII CATG 1 cut(s) 282
NlaIV GGNNCC 5 cut(s) 20, 29, 608, 709, 861
NmeAIII GCCGAG 1 cut(s) 661
PaeR7I CTCGAG 1 cut(s) 670
PcsI WCGNNNNNNNCGW 1 cut(s) 668
PkrI GCNGC 1 cut(s) 924
Ple19I CGATCG 1 cut(s) 1031
PleI GAGTC 2 cut(s) 82, 273
PmaCI CACGTG 1 cut(s) 820
PmlI CACGTG 1 cut(s) 820
PpsI GAGTC 2 cut(s) 82, 273
Ppu21I YACGTR 1 cut(s) 820
PpuMI RGGWCCY 1 cut(s) 876
PshAI GACNNNNGTC 1 cut(s) 754
PshBI ATTAAT 1 cut(s) 252
PsiI TTATAA 1 cut(s) 534
Psp124BI GAGCTC 2 cut(s) 495, 682
Psp5II RGGWCCY 1 cut(s) 876
Psp6I CCWGG 1 cut(s) 21
PspCI CACGTG 1 cut(s) 820
PspGI CCWGG 1 cut(s) 21
PspN4I GGNNCC 5 cut(s) 20, 29, 608, 709, 861
PspPI GGNCC 6 cut(s) 59, 386, 684, 708, 859, 876
PspPPI RGGWCCY 1 cut(s) 876
PsuI RGATCY 3 cut(s) 18, 27, 87
PvuI CGATCG 1 cut(s) 1031
RsaI GTAC 1 cut(s) 50
RsaNI GTAC 1 cut(s) 49
SacI GAGCTC 2 cut(s) 495, 682
SaqAI TTAA 4 cut(s) 252, 308, 321, 453
SatI GCNGC 1 cut(s) 923
Sau3AI GATC 5 cut(s) 18, 27, 87, 347, 1028
Sau96I GGNCC 6 cut(s) 59, 386, 684, 708, 859, 876
SchI GAGTC 2 cut(s) 83, 274
ScrFI CCNGG 8 cut(s) 23, 69, 630, 687, 804, 805, 863, 995
SduI GDGCHC 2 cut(s) 495, 682
SfaNI GCATC 1 cut(s) 818
SfcI CTRYAG 1 cut(s) 723
Sfr274I CTCGAG 1 cut(s) 670
SinI GGWCC 4 cut(s) 386, 708, 859, 876
SlaI CTCGAG 1 cut(s) 670
SmaI CCCGGG 1 cut(s) 805
SmlI CTYRAG 4 cut(s) 452, 670, 871, 940
SmoI CTYRAG 4 cut(s) 452, 670, 871, 940
Sse9I AATT 3 cut(s) 200, 433, 459
SsiI CCGC 4 cut(s) 81, 610, 711, 831
SspI AATATT 1 cut(s) 164
SspMI CTAG 3 cut(s) 138, 177, 293
SstI GAGCTC 2 cut(s) 495, 682
StyD4I CCNGG 8 cut(s) 21, 67, 628, 685, 802, 803, 861, 993
StyI CCWWGG 1 cut(s) 602
TaaI ACNGT 2 cut(s) 131, 919
TaiI ACGT 3 cut(s) 238, 822, 853
TaqI TCGA 5 cut(s) 10, 45, 671, 813, 1027
TasI AATT 3 cut(s) 200, 433, 459
Tru1I TTAA 4 cut(s) 252, 308, 321, 453
Tru9I TTAA 4 cut(s) 252, 308, 321, 453
TseI GCWGC 1 cut(s) 922
TspDTI ATGAA 3 cut(s) 156, 419, 483
TspGWI ACGGA 2 cut(s) 82, 784
TspMI CCCGGG 1 cut(s) 803
Vha464I CTTAAG 1 cut(s) 452
VpaK11BI GGWCC 4 cut(s) 386, 708, 859, 876
VspI ATTAAT 1 cut(s) 252
XhoI CTCGAG 1 cut(s) 670
XmaI CCCGGG 1 cut(s) 803
XspI CTAG 3 cut(s) 138, 177, 293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.