Rroxscaffold_4G00326200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0010610
Physical Location & Seq
Forward (+)
58181319 .. 58182611
1293 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00326200.1

Sequence Viewer

Length: 630 bp
ATGAAGAGTGTACATGTATTGATGTATGGGCATGAATGTATATTAATCGCTGATATGAGGGCGACCTGGGGTATTCCGGACACCGTGCTATTACGCCCTTTGAGAGGTGGCGAGCACTTTAGTCACCCCGAGATGGGGTGGGCGTGCTTTTACGAGTATCGGCTCAAGTGTGGGTTGACGTTTCCGATCGAAGGAGTTGCGGCCGAGGTAGATACCGATGCCATTATCGAGGAGTTGTTGCTAGAGTCGGAGACGGCAAGGCGGCATGACATCGAGCTTCCCAATAAGAAAGTACATGTGACTACCGATCACGGGGTGAACATATTGCGCCGGATGAGATGTAACCGAGGTTTCCTAACGGTGTGTTGGAGTAACTCAACAGCAAGCCGAGGAGTAGGCAGTGCCGAGGTGGAGCGTGGGGCCATGAATGTGCCGTCGTATCCGGTGGTCGAGGTGGAGATCTCCATTGAGGAGTTGTTTGAGGAAGCCGAACGACGCCGTAGCGATACCGGTGCCGTGGCTCGCCCGGTACAAGATGTTGTGTTAGAGCAGTGGACGCTATTTTTGGTGGCGACATTGGTGATCCGGTGTCACGAGAAGTTATTACCGTCCACGGGGAAGCCGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

209

Amino Acids

23.6

Weight (kDa)

5.78

Isoelectric Point (pI)

55.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 512
AccIII TCCGGA 1 cut(s) 76
AciI CCGC 2 cut(s) 200, 262
AclWI GGATC 1 cut(s) 577
AcoI YGGCCR 1 cut(s) 201
AcyI GRCGYC 1 cut(s) 496
AdeI CACNNNGTG 1 cut(s) 316
AfaI GTAC 3 cut(s) 12, 294, 531
AfiI CCNNNNNNNGG 7 cut(s) 104, 133, 134, 135, 191, 312, 614
AflIII ACRYGT 2 cut(s) 13, 295
AgeI ACCGGT 1 cut(s) 509
AjnI CCWGG 1 cut(s) 65
AluBI AGCT 1 cut(s) 277
AluI AGCT 1 cut(s) 277
Alw21I GWGCWC 1 cut(s) 117
Alw26I GTCTC 1 cut(s) 245
AlwI GGATC 1 cut(s) 577
Ama87I CYCGRG 1 cut(s) 128
Aor13HI TCCGGA 1 cut(s) 76
AoxI GGCC 2 cut(s) 201, 420
ArsI GACNNNNNNTTYG 2 cut(s) 547, 579
AseI ATTAAT 1 cut(s) 44
AsiGI ACCGGT 1 cut(s) 509
AspLEI GCGC 1 cut(s) 330
AspS9I GGNCC 1 cut(s) 420
AsuC2I CCSGG 1 cut(s) 527
AsuHPI GGTGA 3 cut(s) 116, 328, 592
AvaI CYCGRG 1 cut(s) 128
BanI GGYRCC 1 cut(s) 512
BauI CACGAG 1 cut(s) 593
Bbv12I GWGCWC 1 cut(s) 117
BccI CCATC 1 cut(s) 127
BceAI ACGGC 4 cut(s) 270, 418, 483, 500
BcgI CGANNNNNNTGC 4 cut(s) 179, 213, 494, 528
BciT130I CCWGG 1 cut(s) 67
BciVI GTATCC 1 cut(s) 450
BcnI CCSGG 1 cut(s) 527
BcoDI GTCTC 1 cut(s) 245
BfaI CTAG 1 cut(s) 242
BfuI GTATCC 1 cut(s) 450
BglII AGATCT 1 cut(s) 459
BisI GCNGC 2 cut(s) 201, 263
BlsI GCNGC 2 cut(s) 202, 264
Bme1390I CCNGG 2 cut(s) 67, 527
BmeT110I CYCGRG 1 cut(s) 128
BmgT120I GGNCC 1 cut(s) 420
BmiI GGNNCC 2 cut(s) 421, 514
BmrFI CCNGG 2 cut(s) 67, 527
BmsI GCATC 1 cut(s) 208
BpuEI CTTGAG 1 cut(s) 149
BpuMI CCSGG 1 cut(s) 527
BsaHI GRCGYC 1 cut(s) 496
BsaJI CCNNGG 8 cut(s) 66, 204, 346, 388, 405, 516, 612, 623
BsaWI WCCGGW 4 cut(s) 76, 442, 509, 585
Bsc4I CCNNNNNNNGG 7 cut(s) 104, 133, 134, 135, 191, 312, 614
Bse118I RCCGGY 1 cut(s) 509
BseAI TCCGGA 1 cut(s) 76
BseBI CCWGG 1 cut(s) 67
BseDI CCNNGG 8 cut(s) 66, 204, 346, 388, 405, 516, 612, 623
BseGI GGATG 1 cut(s) 339
BseLI CCNNNNNNNGG 7 cut(s) 104, 133, 134, 135, 191, 312, 614
BseRI GAGGAG 3 cut(s) 245, 405, 485
BseX3I CGGCCG 1 cut(s) 201
Bsh1285I CGRYCG 2 cut(s) 189, 204
BshFI GGCC 2 cut(s) 203, 422
BshNI GGYRCC 1 cut(s) 512
BshTI ACCGGT 1 cut(s) 509
BsiEI CGRYCG 2 cut(s) 189, 204
BsiHKAI GWGCWC 1 cut(s) 117
BsiHKCI CYCGRG 1 cut(s) 128
BsiSI CCGG 6 cut(s) 77, 331, 443, 510, 527, 586
BslI CCNNNNNNNGG 7 cut(s) 104, 133, 134, 135, 191, 312, 614
BsmAI GTCTC 1 cut(s) 245
BsmBI CGTCTC 1 cut(s) 245
BsnI GGCC 2 cut(s) 203, 422
BsoBI CYCGRG 1 cut(s) 128
Bsp1286I GDGCHC 1 cut(s) 117
Bsp13I TCCGGA 1 cut(s) 76
Bsp1407I TGTACA 1 cut(s) 10
Bsp143I GATC 4 cut(s) 186, 307, 459, 582
BspACI CCGC 2 cut(s) 200, 262
BspANI GGCC 2 cut(s) 203, 422
BspEI TCCGGA 1 cut(s) 76
BspLI GGNNCC 2 cut(s) 421, 514
BspPI GGATC 1 cut(s) 577
BspT107I GGYRCC 1 cut(s) 512
BsrFI RCCGGY 1 cut(s) 509
BsrGI TGTACA 1 cut(s) 10
BssAI RCCGGY 1 cut(s) 509
BssECI CCNNGG 8 cut(s) 66, 204, 346, 388, 405, 516, 612, 623
BssMI GATC 4 cut(s) 186, 307, 459, 582
BssNI GRCGYC 1 cut(s) 496
BssSI CACGAG 1 cut(s) 593
Bst2BI CACGAG 1 cut(s) 593
Bst2UI CCWGG 1 cut(s) 67
Bst4CI ACNGT 3 cut(s) 85, 361, 609
BstACI GRCGYC 1 cut(s) 496
BstAUI TGTACA 1 cut(s) 10
BstC8I GCNNGC 4 cut(s) 113, 145, 385, 523
BstDSI CCRYGG 2 cut(s) 516, 612
BstENI CCTNNNNNAGG 1 cut(s) 102
BstF5I GGATG 1 cut(s) 339
BstHHI GCGC 1 cut(s) 330
BstKTI GATC 4 cut(s) 189, 310, 462, 585
BstMAI GTCTC 1 cut(s) 245
BstMBI GATC 4 cut(s) 186, 307, 459, 582
BstMCI CGRYCG 2 cut(s) 189, 204
BstMWI GCNNNNNNNGC 1 cut(s) 556
BstNI CCWGG 1 cut(s) 67
BstNSI RCATGY 2 cut(s) 17, 299
BstSCI CCNGG 2 cut(s) 65, 525
BstX2I RGATCY 1 cut(s) 459
BstYI RGATCY 1 cut(s) 459
BstZI CGGCCG 1 cut(s) 201
BsuI GTATCC 1 cut(s) 450
BsuRI GGCC 2 cut(s) 203, 422
BtgI CCRYGG 2 cut(s) 516, 612
BtsCI GGATG 1 cut(s) 339
BtsI GCAGTG 2 cut(s) 406, 557
BtsIMutI CAGTG 2 cut(s) 406, 557
Cac8I GCNNGC 4 cut(s) 113, 145, 385, 523
CfoI GCGC 1 cut(s) 330
Cfr10I RCCGGY 1 cut(s) 509
Cfr13I GGNCC 1 cut(s) 420
CseI GACGC 2 cut(s) 504, 565
Csp6I GTAC 3 cut(s) 11, 293, 530
CspAI ACCGGT 1 cut(s) 509
CviAII CATG 5 cut(s) 14, 32, 266, 296, 424
CviJI RGCY 8 cut(s) 163, 203, 277, 387, 422, 488, 521, 622
CviKI_1 RGCY 8 cut(s) 163, 203, 277, 387, 422, 488, 521, 622
CviQI GTAC 3 cut(s) 11, 293, 530
DpnI GATC 4 cut(s) 188, 309, 461, 584
DpnII GATC 4 cut(s) 186, 307, 459, 582
DraIII CACNNNGTG 1 cut(s) 316
EaeI YGGCCR 1 cut(s) 201
EagI CGGCCG 1 cut(s) 201
EclXI CGGCCG 1 cut(s) 201
Eco52I CGGCCG 1 cut(s) 201
Eco88I CYCGRG 1 cut(s) 128
EcoNI CCTNNNNNAGG 1 cut(s) 102
EcoRII CCWGG 1 cut(s) 65
Esp3I CGTCTC 1 cut(s) 245
FaeI CATG 5 cut(s) 17, 35, 269, 299, 427
FaiI YATR 9 cut(s) 15, 27, 33, 41, 56, 267, 297, 323, 425
FatI CATG 5 cut(s) 13, 31, 265, 295, 423
Fnu4HI GCNGC 2 cut(s) 201, 263
FokI GGATG 1 cut(s) 346
Fsp4HI GCNGC 2 cut(s) 201, 263
FspBI CTAG 1 cut(s) 242
GlaI GCGC 1 cut(s) 329
GluI GCNGC 2 cut(s) 201, 263
HaeIII GGCC 2 cut(s) 203, 422
HapII CCGG 6 cut(s) 77, 331, 443, 510, 527, 586
HgaI GACGC 2 cut(s) 504, 565
HhaI GCGC 1 cut(s) 330
Hin1I GRCGYC 1 cut(s) 496
Hin1II CATG 5 cut(s) 17, 35, 269, 299, 427
Hin6I GCGC 1 cut(s) 328
HinP1I GCGC 1 cut(s) 328
HincII GTYRAC 1 cut(s) 177
HindII GTYRAC 1 cut(s) 177
HinfI GANTC 1 cut(s) 245
HpaII CCGG 6 cut(s) 77, 331, 443, 510, 527, 586
HphI GGTGA 3 cut(s) 116, 328, 592
Hpy166II GTNNAC 5 cut(s) 11, 177, 319, 555, 612
Hpy188I TCNGA 2 cut(s) 186, 250
Hpy188III TCNNGA 2 cut(s) 77, 593
Hpy8I GTNNAC 5 cut(s) 11, 177, 319, 555, 612
Hpy99I CGWCG 2 cut(s) 439, 498
HpyAV CCTTC 1 cut(s) 185
HpyCH4III ACNGT 3 cut(s) 85, 361, 609
HpyCH4IV ACGT 1 cut(s) 179
HpyF10VI GCNNNNNNNGC 1 cut(s) 556
HpySE526I ACGT 1 cut(s) 179
Hsp92I GRCGYC 1 cut(s) 496
Hsp92II CATG 5 cut(s) 17, 35, 269, 299, 427
HspAI GCGC 1 cut(s) 328
Kpn2I TCCGGA 1 cut(s) 76
Kzo9I GATC 4 cut(s) 186, 307, 459, 582
LmnI GCTCC 1 cut(s) 412
LpnPI CCDG 8 cut(s) 52, 79, 90, 344, 456, 523, 540, 599
LweI GCATC 1 cut(s) 208
MaeI CTAG 1 cut(s) 242
MaeII ACGT 1 cut(s) 179
MaeIII GTNAC 5 cut(s) 122, 298, 341, 371, 590
MalI GATC 4 cut(s) 188, 309, 461, 584
MboI GATC 4 cut(s) 186, 307, 459, 582
MboII GAAGA 1 cut(s) 16
MflI RGATCY 1 cut(s) 459
MhlI GDGCHC 1 cut(s) 117
MlyI GAGTC 1 cut(s) 254
MmeI TCCRAC 2 cut(s) 228, 347
MroI TCCGGA 1 cut(s) 76
MseI TTAA 1 cut(s) 44
MslI CAYNNNNRTG 1 cut(s) 428
MspI CCGG 6 cut(s) 77, 331, 443, 510, 527, 586
MspR9I CCNGG 2 cut(s) 67, 527
MvaI CCWGG 1 cut(s) 67
MwoI GCNNNNNNNGC 1 cut(s) 556
NciI CCSGG 1 cut(s) 527
NdeII GATC 4 cut(s) 186, 307, 459, 582
NlaIII CATG 5 cut(s) 17, 35, 269, 299, 427
NlaIV GGNNCC 2 cut(s) 421, 514
NmeAIII GCCGAG 3 cut(s) 229, 413, 430
NmuCI GTSAC 3 cut(s) 122, 298, 590
NspI RCATGY 2 cut(s) 17, 299
PciI ACATGT 2 cut(s) 13, 295
PcsI WCGNNNNNNNCGW 1 cut(s) 620
PinAI ACCGGT 1 cut(s) 509
PkrI GCNGC 2 cut(s) 202, 264
Ple19I CGATCG 1 cut(s) 189
PleI GAGTC 1 cut(s) 253
PpsI GAGTC 1 cut(s) 253
PscI ACATGT 2 cut(s) 13, 295
PshBI ATTAAT 1 cut(s) 44
Psp6I CCWGG 1 cut(s) 65
PspGI CCWGG 1 cut(s) 65
PspN4I GGNNCC 2 cut(s) 421, 514
PspPI GGNCC 1 cut(s) 420
PsuI RGATCY 1 cut(s) 459
PvuI CGATCG 1 cut(s) 189
RsaI GTAC 3 cut(s) 12, 294, 531
RsaNI GTAC 3 cut(s) 11, 293, 530
RseI CAYNNNNRTG 1 cut(s) 428
SaqAI TTAA 1 cut(s) 44
SatI GCNGC 2 cut(s) 201, 263
Sau3AI GATC 4 cut(s) 186, 307, 459, 582
Sau96I GGNCC 1 cut(s) 420
SchI GAGTC 1 cut(s) 254
ScrFI CCNGG 2 cut(s) 67, 527
SduI GDGCHC 1 cut(s) 117
SetI ASST 9 cut(s) 68, 109, 182, 210, 279, 352, 411, 456, 629
SfaNI GCATC 1 cut(s) 208
SmiMI CAYNNNNRTG 1 cut(s) 428
SmlI CTYRAG 1 cut(s) 164
SmoI CTYRAG 1 cut(s) 164
SsiI CCGC 2 cut(s) 200, 262
SspMI CTAG 1 cut(s) 242
StyD4I CCNGG 2 cut(s) 65, 525
TaaI ACNGT 3 cut(s) 85, 361, 609
TaiI ACGT 1 cut(s) 182
TaqI TCGA 4 cut(s) 189, 228, 273, 450
TatI WGTACW 2 cut(s) 10, 292
TauI GCSGC 2 cut(s) 203, 265
Tru1I TTAA 1 cut(s) 44
Tru9I TTAA 1 cut(s) 44
TscAI CASTG 2 cut(s) 406, 557
TseFI GTSAC 3 cut(s) 122, 298, 590
Tsp45I GTSAC 3 cut(s) 122, 298, 590
TspDTI ATGAA 3 cut(s) 17, 48, 440
TspRI CASTG 2 cut(s) 406, 557
VspI ATTAAT 1 cut(s) 44
XagI CCTNNNNNAGG 1 cut(s) 102
XceI RCATGY 2 cut(s) 17, 299
XspI CTAG 1 cut(s) 242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.