Rmu_sc0004711.1_g000018

Polyketide cyclase / dehydrase and lipid transport

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004711.1
OG0008041
Physical Location & Seq
Forward (+)
76571 .. 78003
1433 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 840 bp
atgtccttgaaatcaatctgcttctctctttggctccctagcccttctccgagtaccgctgctcctgcttcaatctcttccccctattcctttcctcggagaaatccaaaccactccgtctccaattccggttcttcaaggccgcacctgctgcatttctcgaccaagctcgactccgcgttcttgtctgaggatgacgattacgatgacgattccgattcagaagcagagaaatcggaagttgatatagatgtagatatagagttagagatagagaagacgggggccaactgccggagaatccgatcggaaatccgaatagaggcgccactcgccaccgtctggaacttgttgacggattacgagagactggccgacttcattcccggactcgccgtctgccagttgctccagaagtcggagaactacgctcggctttttcagattggggagcaggacttggcgtttgggctgaaattcaacgcgaaaggagtggtggattgttttgagaatcctctagagagcattgctaatgatttggggggtctcactctgaagcgtgatattgagtttaacatggtcgaaggtgactttgaaatctttcgaggaaagtggtctctccaacagcttaacactagtggggaaatggcatgtgatcctttgatagaccagaaaatgcaaacaacaaccagtctttcctacttggttgatgtaaagccgaagatgttgttgccggttcagctggttgagggtagactctgcaaggagatcaaaataaacctagcttgcatccgggaagaagcactgaaaatgactcgcgaaactcttcttactcgttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polyketide_cyc

Protein Analysis

279

Amino Acids

31.33

Weight (kDa)

4.75

Isoelectric Point (pI)

56.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Polyketide_cyc PF03364 107 - 255 1.3e-18 Polyketide cyclase / dehydrase and lipid transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 156
Acc36I ACCTGC 1 cut(s) 156
AccB1I GGYRCC 1 cut(s) 325
AccB7I CCANNNNNTGG 1 cut(s) 342
AccI GTMKAC 1 cut(s) 754
AccII CGCG 3 cut(s) 179, 485, 819
AciI CCGC 3 cut(s) 57, 143, 177
AclWI GGATC 1 cut(s) 650
AcoI YGGCCR 1 cut(s) 372
AcsI RAATTY 1 cut(s) 476
AcuI CTGAAG 1 cut(s) 575
AcyI GRCGYC 1 cut(s) 326
AfaI GTAC 1 cut(s) 55
AfiI CCNNNNNNNGG 5 cut(s) 96, 294, 322, 342, 418
AgsI TTSAA 5 cut(s) 10, 72, 138, 481, 596
AhdI GACNNNNNGTC 1 cut(s) 395
AhlI ACTAGT 1 cut(s) 635
AluBI AGCT 4 cut(s) 169, 628, 742, 785
AluI AGCT 4 cut(s) 169, 628, 742, 785
Alw26I GTCTC 4 cut(s) 124, 361, 551, 621
AlwI GGATC 1 cut(s) 650
AoxI GGCC 3 cut(s) 140, 285, 372
ApeKI GCWGC 2 cut(s) 59, 151
ApoI RAATTY 1 cut(s) 476
ArsI GACNNNNNNTTYG 2 cut(s) 449, 481
Asp700I GAANNNNTTC 2 cut(s) 600, 825
AspLEI GCGC 1 cut(s) 328
AspS9I GGNCC 1 cut(s) 285
AsuC2I CCSGG 2 cut(s) 387, 794
AsuHPI GGTGA 1 cut(s) 599
BanI GGYRCC 1 cut(s) 325
BbsI GAAGAC 1 cut(s) 284
BbvI GCAGC 2 cut(s) 46, 138
BceAI ACGGC 1 cut(s) 380
BcgI CGANNNNNNTGC 2 cut(s) 216, 250
BcnI CCSGG 2 cut(s) 387, 794
BcoDI GTCTC 4 cut(s) 124, 361, 551, 621
BcuI ACTAGT 1 cut(s) 635
BfaI CTAG 4 cut(s) 39, 518, 636, 782
BfoI RGCGCY 1 cut(s) 329
BfuAI ACCTGC 1 cut(s) 156
BisI GCNGC 3 cut(s) 60, 143, 152
BlsI GCNGC 3 cut(s) 61, 144, 153
Bme1390I CCNGG 2 cut(s) 387, 794
BmeRI GACNNNNNGTC 1 cut(s) 395
BmgT120I GGNCC 1 cut(s) 285
BmiI GGNNCC 3 cut(s) 35, 286, 327
BmrFI CCNGG 2 cut(s) 387, 794
BmsI GCATC 1 cut(s) 798
BpiI GAAGAC 1 cut(s) 284
BpmI CTGGAG 1 cut(s) 395
BpuMI CCSGG 2 cut(s) 387, 794
BsaHI GRCGYC 1 cut(s) 326
BsaI GGTCTC 2 cut(s) 551, 621
BsaJI CCNNGG 1 cut(s) 95
BsaWI WCCGGW 1 cut(s) 128
BsaXI ACNNNNNCTCC 4 cut(s) 46, 76, 104, 134
Bsc4I CCNNNNNNNGG 5 cut(s) 96, 294, 322, 342, 418
Bse118I RCCGGY 1 cut(s) 733
Bse1I ACTGG 3 cut(s) 375, 403, 690
Bse3DI GCAATG 1 cut(s) 525
BseDI CCNNGG 1 cut(s) 95
BseGI GGATG 2 cut(s) 199, 789
BseLI CCNNNNNNNGG 5 cut(s) 96, 294, 322, 342, 418
BseMI GCAATG 1 cut(s) 525
BseMII CTCAG 1 cut(s) 180
BseNI ACTGG 3 cut(s) 375, 403, 690
BseXI GCAGC 2 cut(s) 46, 138
Bsh1236I CGCG 3 cut(s) 179, 485, 819
Bsh1285I CGRYCG 1 cut(s) 308
BshFI GGCC 3 cut(s) 142, 287, 374
BshNI GGYRCC 1 cut(s) 325
BsiEI CGRYCG 1 cut(s) 308
BsiSI CCGG 5 cut(s) 129, 295, 387, 734, 793
BslI CCNNNNNNNGG 5 cut(s) 96, 294, 322, 342, 418
BsmAI GTCTC 4 cut(s) 124, 361, 551, 621
BsmBI CGTCTC 1 cut(s) 124
BsnI GGCC 3 cut(s) 142, 287, 374
Bso31I GGTCTC 2 cut(s) 551, 621
Bsp143I GATC 3 cut(s) 305, 655, 768
Bsp68I TCGCGA 1 cut(s) 819
BspACI CCGC 3 cut(s) 57, 143, 177
BspANI GGCC 3 cut(s) 142, 287, 374
BspCNI CTCAG 1 cut(s) 181
BspFNI CGCG 3 cut(s) 179, 485, 819
BspLI GGNNCC 3 cut(s) 35, 286, 327
BspMI ACCTGC 1 cut(s) 156
BspPI GGATC 1 cut(s) 650
BspT107I GGYRCC 1 cut(s) 325
BspTNI GGTCTC 2 cut(s) 551, 621
BsrDI GCAATG 1 cut(s) 525
BsrFI RCCGGY 1 cut(s) 733
BsrI ACTGG 3 cut(s) 375, 403, 690
BssAI RCCGGY 1 cut(s) 733
BssECI CCNNGG 1 cut(s) 95
BssMI GATC 3 cut(s) 305, 655, 768
BssNI GRCGYC 1 cut(s) 326
Bst4CI ACNGT 1 cut(s) 340
Bst6I CTCTTC 2 cut(s) 82, 831
BstACI GRCGYC 1 cut(s) 326
BstAPI GCANNNNNTGC 1 cut(s) 151
BstC8I GCNNGC 1 cut(s) 787
BstDEI CTNAG 1 cut(s) 189
BstF5I GGATG 2 cut(s) 199, 789
BstFNI CGCG 3 cut(s) 179, 485, 819
BstH2I RGCGCY 1 cut(s) 329
BstHHI GCGC 1 cut(s) 328
BstKTI GATC 3 cut(s) 308, 658, 771
BstMAI GTCTC 4 cut(s) 124, 361, 551, 621
BstMBI GATC 3 cut(s) 305, 655, 768
BstMCI CGRYCG 1 cut(s) 308
BstMWI GCNNNNNNNGC 5 cut(s) 65, 148, 151, 332, 739
BstNSI RCATGY 1 cut(s) 654
BstSCI CCNGG 2 cut(s) 385, 792
BstUI CGCG 3 cut(s) 179, 485, 819
BstV1I GCAGC 2 cut(s) 46, 138
BstV2I GAAGAC 1 cut(s) 284
BsuRI GGCC 3 cut(s) 142, 287, 374
BtsCI GGATG 2 cut(s) 199, 789
BtsIMutI CAGTG 1 cut(s) 803
BtuMI TCGCGA 1 cut(s) 819
BveI ACCTGC 1 cut(s) 156
Cac8I GCNNGC 1 cut(s) 787
CfoI GCGC 1 cut(s) 328
Cfr10I RCCGGY 1 cut(s) 733
Cfr13I GGNCC 1 cut(s) 285
Csp6I GTAC 1 cut(s) 54
CviAII CATG 2 cut(s) 577, 651
CviQI GTAC 1 cut(s) 54
DdeI CTNAG 1 cut(s) 189
DinI GGCGCC 1 cut(s) 327
DpnI GATC 3 cut(s) 307, 657, 770
DpnII GATC 3 cut(s) 305, 655, 768
DriI GACNNNNNGTC 1 cut(s) 395
EaeI YGGCCR 1 cut(s) 372
Eam1104I CTCTTC 2 cut(s) 82, 831
Eam1105I GACNNNNNGTC 1 cut(s) 395
EarI CTCTTC 2 cut(s) 82, 831
Eco31I GGTCTC 2 cut(s) 551, 621
Eco57I CTGAAG 1 cut(s) 575
EgeI GGCGCC 1 cut(s) 327
EheI GGCGCC 1 cut(s) 327
Esp3I CGTCTC 1 cut(s) 124
FaeI CATG 2 cut(s) 580, 654
FaiI YATR 4 cut(s) 248, 260, 578, 652
FatI CATG 2 cut(s) 576, 650
FblI GTMKAC 1 cut(s) 754
Fnu4HI GCNGC 3 cut(s) 60, 143, 152
FokI GGATG 2 cut(s) 206, 776
Fsp4HI GCNGC 3 cut(s) 60, 143, 152
FspBI CTAG 4 cut(s) 39, 518, 636, 782
GlaI GCGC 1 cut(s) 327
GluI GCNGC 3 cut(s) 60, 143, 152
GsuI CTGGAG 1 cut(s) 395
HaeII RGCGCY 1 cut(s) 329
HaeIII GGCC 3 cut(s) 142, 287, 374
HapII CCGG 5 cut(s) 129, 295, 387, 734, 793
HhaI GCGC 1 cut(s) 328
Hin1I GRCGYC 1 cut(s) 326
Hin1II CATG 2 cut(s) 580, 654
Hin6I GCGC 1 cut(s) 326
HinP1I GCGC 1 cut(s) 326
HincII GTYRAC 1 cut(s) 354
HindII GTYRAC 1 cut(s) 354
HinfI GANTC 8 cut(s) 173, 212, 218, 300, 390, 511, 756, 814
HpaII CCGG 5 cut(s) 129, 295, 387, 734, 793
HphI GGTGA 1 cut(s) 599
Hpy166II GTNNAC 2 cut(s) 354, 755
Hpy188III TCNNGA 5 cut(s) 160, 343, 412, 518, 818
Hpy8I GTNNAC 2 cut(s) 354, 755
HpyAV CCTTC 2 cut(s) 54, 578
HpyCH4III ACNGT 1 cut(s) 340
HpyCH4V TGCA 4 cut(s) 154, 679, 762, 789
HpyF10VI GCNNNNNNNGC 5 cut(s) 65, 148, 151, 332, 739
HpyF3I CTNAG 1 cut(s) 189
Hsp92I GRCGYC 1 cut(s) 326
Hsp92II CATG 2 cut(s) 580, 654
HspAI GCGC 1 cut(s) 326
KasI GGCGCC 1 cut(s) 325
Kzo9I GATC 3 cut(s) 305, 655, 768
LmnI GCTCC 4 cut(s) 39, 67, 414, 451
Lsp1109I GCAGC 2 cut(s) 46, 138
LweI GCATC 1 cut(s) 798
MaeI CTAG 4 cut(s) 39, 518, 636, 782
MaeIII GTNAC 1 cut(s) 587
MalI GATC 3 cut(s) 307, 657, 770
MboI GATC 3 cut(s) 305, 655, 768
MboII GAAGA 6 cut(s) 69, 126, 289, 733, 809, 818
MluCI AATT 2 cut(s) 124, 476
Mly113I GGCGCC 1 cut(s) 326
MlyI GAGTC 4 cut(s) 167, 384, 750, 808
MmeI TCCRAC 2 cut(s) 399, 646
MnlI CCTC 6 cut(s) 105, 184, 316, 525, 599, 742
MroXI GAANNNNTTC 2 cut(s) 600, 825
MseI TTAA 2 cut(s) 573, 630
MspA1I CMGCKG 2 cut(s) 59, 742
MspI CCGG 5 cut(s) 129, 295, 387, 734, 793
MspR9I CCNGG 2 cut(s) 387, 794
MvnI CGCG 3 cut(s) 179, 485, 819
MwoI GCNNNNNNNGC 5 cut(s) 65, 148, 151, 332, 739
NarI GGCGCC 1 cut(s) 326
NciI CCSGG 2 cut(s) 387, 794
NdeII GATC 3 cut(s) 305, 655, 768
NlaIII CATG 2 cut(s) 580, 654
NlaIV GGNNCC 3 cut(s) 35, 286, 327
NmeAIII GCCGAG 1 cut(s) 412
NmuCI GTSAC 1 cut(s) 587
NruI TCGCGA 1 cut(s) 819
NspI RCATGY 1 cut(s) 654
PaqCI CACCTGC 1 cut(s) 156
PdmI GAANNNNTTC 2 cut(s) 600, 825
PfeI GAWTC 4 cut(s) 212, 218, 300, 511
PflMI CCANNNNNTGG 1 cut(s) 342
PfoI TCCNGGA 2 cut(s) 385, 792
PkrI GCNGC 3 cut(s) 61, 144, 153
Ple19I CGATCG 1 cut(s) 308
PleI GAGTC 4 cut(s) 167, 384, 750, 808
PluTI GGCGCC 1 cut(s) 329
PpsI GAGTC 4 cut(s) 167, 384, 750, 808
PspN4I GGNNCC 3 cut(s) 35, 286, 327
PspPI GGNCC 1 cut(s) 285
PvuI CGATCG 1 cut(s) 308
PvuII CAGCTG 1 cut(s) 742
RruI TCGCGA 1 cut(s) 819
RsaI GTAC 1 cut(s) 55
RsaNI GTAC 1 cut(s) 54
SaqAI TTAA 2 cut(s) 573, 630
SatI GCNGC 3 cut(s) 60, 143, 152
Sau3AI GATC 3 cut(s) 305, 655, 768
Sau96I GGNCC 1 cut(s) 285
SchI GAGTC 4 cut(s) 167, 384, 750, 808
ScrFI CCNGG 2 cut(s) 387, 794
SetI ASST 7 cut(s) 150, 171, 589, 630, 744, 783, 787
SfaNI GCATC 1 cut(s) 798
SfoI GGCGCC 1 cut(s) 327
SpeI ACTAGT 1 cut(s) 635
Sse9I AATT 2 cut(s) 124, 476
SsiI CCGC 3 cut(s) 57, 143, 177
SspDI GGCGCC 1 cut(s) 325
SspMI CTAG 4 cut(s) 39, 518, 636, 782
StyD4I CCNGG 2 cut(s) 385, 792
TaaI ACNGT 1 cut(s) 340
TaqI TCGA 4 cut(s) 161, 171, 582, 604
TasI AATT 2 cut(s) 124, 476
TauI GCSGC 1 cut(s) 145
TfiI GAWTC 4 cut(s) 212, 218, 300, 511
Tru1I TTAA 2 cut(s) 573, 630
Tru9I TTAA 2 cut(s) 573, 630
TscAI CASTG 1 cut(s) 810
TseFI GTSAC 1 cut(s) 587
TseI GCWGC 2 cut(s) 59, 151
Tsp45I GTSAC 1 cut(s) 587
TspDTI ATGAA 1 cut(s) 370
TspGWI ACGGA 2 cut(s) 106, 371
TspRI CASTG 1 cut(s) 810
Van91I CCANNNNNTGG 1 cut(s) 342
XapI RAATTY 1 cut(s) 476
XbaI TCTAGA 1 cut(s) 517
XceI RCATGY 1 cut(s) 654
XmiI GTMKAC 1 cut(s) 754
XmnI GAANNNNTTC 2 cut(s) 600, 825
XspI CTAG 4 cut(s) 39, 518, 636, 782
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.