Rmu_sc0004728.1_g000006

Component of the coenzyme Q biosynthetic pathway. May play a role in organizing a multi-subunit COQ enzyme complex required for coenzyme Q biosynthesis. Required for steady-state levels of other COQ polypeptides

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004728.1
OG0005151
Physical Location & Seq
Reverse (-)
32727 .. 34181
1455 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 762 bp
atggttagggatttgaaggaaaagagggtagcagccaccatgatcggaggtgctcgtgttcggctgaaccggtggcagcaggcggcggtggcagttgggtcggcagtgggggcattgctggacccaagaagagctgacctgatagcagctctgggggagacaactgggaagccggcattcgaaagaattgttcagagaatgaagagtagccctgaaggcagagccatactgttggagcaaccccgtgtaatatctgcaaatgtgggtcatgcatgggatcttccgcccaatacatttggtgctgcctatgcgaagttcatgggatctaggaacttctctccagatgatcgaccaccagtgcgtttcatggaaacagatgaacttgcatatgtagccatgagggctcgtgaggtgcacgacttctggcacaccctgtttgaccttccaacaaacttgatgggtgagtcagcactcaaagtaatagagtttcagcaaatgtatcttcccatgtgcttgatgtccgctataggaggctctgtgaggttcaatgagaagcaaagaaaattgttcttccagcactactttccttgggccattcaagctggtatgcagtcaacagatctcatgtgtatttattatgagaaacactttcatgagaatttggaggatgttcggagaaagtggggtataactcctgctcctgctgcacctaaacaaacccaagcagcagcagcttcacctggtctagtggagaagccataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Coq4

Protein Analysis

253

Amino Acids

28.43

Weight (kDa)

9.39

Isoelectric Point (pI)

43.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Coq4 PF05019 19 - 234 1.6e-84 Coenzyme Q (ubiquinone) biosynthesis protein Coq4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 83, 86, 284, 522
AclWI GGATC 2 cut(s) 285, 331
AcsI RAATTY 1 cut(s) 658
AcuI CTGAAG 1 cut(s) 234
AgeI ACCGGT 1 cut(s) 69
AgsI TTSAA 3 cut(s) 16, 547, 599
AjnI CCWGG 1 cut(s) 739
AluBI AGCT 4 cut(s) 134, 149, 602, 734
AluI AGCT 4 cut(s) 134, 149, 602, 734
Alw21I GWGCWC 2 cut(s) 55, 417
Alw26I GTCTC 1 cut(s) 152
Alw44I GTGCAC 1 cut(s) 413
AlwI GGATC 2 cut(s) 285, 331
AoxI GGCC 1 cut(s) 591
ApaLI GTGCAC 1 cut(s) 413
ApeKI GCWGC 8 cut(s) 32, 76, 146, 302, 704, 725, 728, 731
ApoI RAATTY 1 cut(s) 658
AsiGI ACCGGT 1 cut(s) 69
AspS9I GGNCC 2 cut(s) 121, 591
AsuHPI GGTGA 2 cut(s) 473, 729
AsuII TTCGAA 1 cut(s) 180
AvaII GGWCC 1 cut(s) 121
BaeGI GKGCMC 1 cut(s) 417
BanII GRGCYC 1 cut(s) 406
BauI CACGAG 2 cut(s) 54, 405
Bbv12I GWGCWC 2 cut(s) 55, 417
BbvI GCAGC 8 cut(s) 44, 88, 158, 289, 691, 737, 740, 743
BccI CCATC 1 cut(s) 451
BcgI CGANNNNNNTGC 2 cut(s) 81, 115
BciT130I CCWGG 1 cut(s) 741
BcoDI GTCTC 1 cut(s) 152
BfaI CTAG 2 cut(s) 327, 746
BfmI CTRYAG 1 cut(s) 525
BglI GCCNNNNNGGC 2 cut(s) 216, 401
BglII AGATCT 1 cut(s) 619
BisI GCNGC 9 cut(s) 33, 77, 84, 147, 303, 705, 726, 729, 732
BlsI GCNGC 9 cut(s) 34, 78, 85, 148, 304, 706, 727, 730, 733
Bme1390I CCNGG 1 cut(s) 741
Bme18I GGWCC 1 cut(s) 121
BmgT120I GGNCC 2 cut(s) 121, 591
BmiI GGNNCC 1 cut(s) 123
BmrFI CCNGG 1 cut(s) 741
BmrI ACTGGG 1 cut(s) 174
BmuI ACTGGG 1 cut(s) 174
BpmI CTGGAG 1 cut(s) 324
Bpu14I TTCGAA 1 cut(s) 180
BsaJI CCNNGG 1 cut(s) 587
BsaWI WCCGGW 1 cut(s) 69
BsaXI ACNNNNNCTCC 4 cut(s) 522, 552, 682, 712
Bse118I RCCGGY 2 cut(s) 69, 172
Bse1I ACTGG 2 cut(s) 169, 356
Bse3DI GCAATG 1 cut(s) 113
BseBI CCWGG 1 cut(s) 741
BseDI CCNNGG 1 cut(s) 587
BseGI GGATG 1 cut(s) 673
BseMI GCAATG 1 cut(s) 113
BseNI ACTGG 2 cut(s) 169, 356
BseSI GKGCMC 1 cut(s) 417
BseXI GCAGC 8 cut(s) 44, 88, 158, 289, 691, 737, 740, 743
BsgI GTGCAG 1 cut(s) 690
BshFI GGCC 1 cut(s) 593
BshTI ACCGGT 1 cut(s) 69
BsiHKAI GWGCWC 2 cut(s) 55, 417
BsiSI CCGG 2 cut(s) 70, 173
BsmAI GTCTC 1 cut(s) 152
BsmI GAATGC 1 cut(s) 176
BsnI GGCC 1 cut(s) 593
Bsp119I TTCGAA 1 cut(s) 180
Bsp1286I GDGCHC 3 cut(s) 55, 406, 417
Bsp143I GATC 5 cut(s) 42, 277, 323, 346, 619
BspACI CCGC 4 cut(s) 83, 86, 284, 522
BspANI GGCC 1 cut(s) 593
BspHI TCATGA 1 cut(s) 652
BspLI GGNNCC 1 cut(s) 123
BspPI GGATC 2 cut(s) 285, 331
BspQI GCTCTTC 1 cut(s) 124
BspT104I TTCGAA 1 cut(s) 180
BsrDI GCAATG 1 cut(s) 113
BsrFI RCCGGY 2 cut(s) 69, 172
BsrI ACTGG 2 cut(s) 169, 356
BssAI RCCGGY 2 cut(s) 69, 172
BssECI CCNNGG 1 cut(s) 587
BssMI GATC 5 cut(s) 42, 277, 323, 346, 619
BssSI CACGAG 2 cut(s) 54, 405
BssT1I CCWWGG 1 cut(s) 587
Bst2BI CACGAG 2 cut(s) 54, 405
Bst2UI CCWGG 1 cut(s) 741
Bst4CI ACNGT 1 cut(s) 231
Bst6I CTCTTC 2 cut(s) 124, 197
BstBI TTCGAA 1 cut(s) 180
BstC8I GCNNGC 2 cut(s) 81, 174
BstF5I GGATG 1 cut(s) 673
BstKTI GATC 5 cut(s) 45, 280, 326, 349, 622
BstMAI GTCTC 1 cut(s) 152
BstMBI GATC 5 cut(s) 42, 277, 323, 346, 619
BstMWI GCNNNNNNNGC 9 cut(s) 89, 110, 216, 308, 392, 401, 599, 704, 731
BstNI CCWGG 1 cut(s) 741
BstSCI CCNGG 1 cut(s) 739
BstSFI CTRYAG 1 cut(s) 525
BstSLI GKGCMC 1 cut(s) 417
BstV1I GCAGC 8 cut(s) 44, 88, 158, 289, 691, 737, 740, 743
BstX2I RGATCY 3 cut(s) 277, 323, 619
BstXI CCANNNNNNTGG 1 cut(s) 232
BstYI RGATCY 3 cut(s) 277, 323, 619
BsuRI GGCC 1 cut(s) 593
BtsCI GGATG 1 cut(s) 673
BtsI GCAGTG 1 cut(s) 111
BtsIMutI CAGTG 2 cut(s) 111, 363
Cac8I GCNNGC 2 cut(s) 81, 174
CciI TCATGA 1 cut(s) 652
Cfr10I RCCGGY 2 cut(s) 69, 172
Cfr13I GGNCC 2 cut(s) 121, 591
CsiI ACCWGGT 1 cut(s) 739
CspAI ACCGGT 1 cut(s) 69
CviAII CATG 9 cut(s) 40, 269, 273, 319, 367, 397, 508, 625, 653
DpnI GATC 5 cut(s) 44, 279, 325, 348, 621
DpnII GATC 5 cut(s) 42, 277, 323, 346, 619
Eam1104I CTCTTC 2 cut(s) 124, 197
EarI CTCTTC 2 cut(s) 124, 197
EciI GGCGGA 1 cut(s) 273
Eco130I CCWWGG 1 cut(s) 587
Eco24I GRGCYC 1 cut(s) 406
Eco47I GGWCC 1 cut(s) 121
Eco57I CTGAAG 1 cut(s) 234
EcoRII CCWGG 1 cut(s) 739
EcoT14I CCWWGG 1 cut(s) 587
EcoT22I ATGCAT 1 cut(s) 274
EcoT38I GRGCYC 1 cut(s) 406
ErhI CCWWGG 1 cut(s) 587
FaeI CATG 9 cut(s) 43, 272, 276, 322, 370, 400, 511, 628, 656
FatI CATG 9 cut(s) 39, 268, 272, 318, 366, 396, 507, 624, 652
FauNDI CATATG 1 cut(s) 388
Fnu4HI GCNGC 9 cut(s) 33, 77, 84, 147, 303, 705, 726, 729, 732
FokI GGATG 1 cut(s) 680
FriOI GRGCYC 1 cut(s) 406
Fsp4HI GCNGC 9 cut(s) 33, 77, 84, 147, 303, 705, 726, 729, 732
FspBI CTAG 2 cut(s) 327, 746
GluI GCNGC 9 cut(s) 33, 77, 84, 147, 303, 705, 726, 729, 732
GsuI CTGGAG 1 cut(s) 324
HaeIII GGCC 1 cut(s) 593
HapII CCGG 2 cut(s) 70, 173
Hin1II CATG 9 cut(s) 43, 272, 276, 322, 370, 400, 511, 628, 656
HincII GTYRAC 1 cut(s) 615
HindII GTYRAC 1 cut(s) 615
HinfI GANTC 1 cut(s) 464
HpaII CCGG 2 cut(s) 70, 173
HphI GGTGA 2 cut(s) 473, 729
Hpy166II GTNNAC 2 cut(s) 415, 615
Hpy188I TCNGA 3 cut(s) 47, 195, 675
Hpy188III TCNNGA 3 cut(s) 341, 407, 653
Hpy8I GTNNAC 2 cut(s) 415, 615
HpyAV CCTTC 3 cut(s) 10, 209, 452
HpyCH4III ACNGT 1 cut(s) 231
HpyCH4V TGCA 6 cut(s) 257, 272, 386, 415, 610, 707
HpyF10VI GCNNNNNNNGC 9 cut(s) 89, 110, 216, 308, 392, 401, 599, 704, 731
Hsp92II CATG 9 cut(s) 43, 272, 276, 322, 370, 400, 511, 628, 656
KroI GCCGGC 1 cut(s) 172
KroNI GCCGGC 1 cut(s) 174
Kzo9I GATC 5 cut(s) 42, 277, 323, 346, 619
LguI GCTCTTC 1 cut(s) 124
LmnI GCTCC 2 cut(s) 235, 703
Lsp1109I GCAGC 8 cut(s) 44, 88, 158, 289, 691, 737, 740, 743
MabI ACCWGGT 1 cut(s) 739
MaeI CTAG 2 cut(s) 327, 746
MalI GATC 5 cut(s) 44, 279, 325, 348, 621
MboI GATC 5 cut(s) 42, 277, 323, 346, 619
MboII GAAGA 5 cut(s) 141, 214, 272, 494, 562
MflI RGATCY 3 cut(s) 277, 323, 619
MhlI GDGCHC 3 cut(s) 55, 406, 417
MluCI AATT 3 cut(s) 186, 563, 658
MlyI GAGTC 1 cut(s) 473
MmeI TCCRAC 2 cut(s) 213, 470
MnlI CCTC 7 cut(s) 18, 41, 393, 403, 524, 534, 658
Mph1103I ATGCAT 1 cut(s) 274
MroNI GCCGGC 1 cut(s) 172
MslI CAYNNNNRTG 1 cut(s) 651
MspI CCGG 2 cut(s) 70, 173
MspR9I CCNGG 1 cut(s) 741
Mva1269I GAATGC 1 cut(s) 176
MvaI CCWGG 1 cut(s) 741
MwoI GCNNNNNNNGC 9 cut(s) 89, 110, 216, 308, 392, 401, 599, 704, 731
NaeI GCCGGC 1 cut(s) 174
NdeI CATATG 1 cut(s) 388
NdeII GATC 5 cut(s) 42, 277, 323, 346, 619
NgoMIV GCCGGC 1 cut(s) 172
NlaIII CATG 9 cut(s) 43, 272, 276, 322, 370, 400, 511, 628, 656
NlaIV GGNNCC 1 cut(s) 123
NsiI ATGCAT 1 cut(s) 274
NspV TTCGAA 1 cut(s) 180
PagI TCATGA 1 cut(s) 652
PciSI GCTCTTC 1 cut(s) 124
PctI GAATGC 1 cut(s) 176
PdiI GCCGGC 1 cut(s) 174
PinAI ACCGGT 1 cut(s) 69
PkrI GCNGC 9 cut(s) 34, 78, 85, 148, 304, 706, 727, 730, 733
PleI GAGTC 1 cut(s) 472
PpsI GAGTC 1 cut(s) 472
Psp6I CCWGG 1 cut(s) 739
PspGI CCWGG 1 cut(s) 739
PspN4I GGNNCC 1 cut(s) 123
PspPI GGNCC 2 cut(s) 121, 591
PsuI RGATCY 3 cut(s) 277, 323, 619
RseI CAYNNNNRTG 1 cut(s) 651
SapI GCTCTTC 1 cut(s) 124
SatI GCNGC 9 cut(s) 33, 77, 84, 147, 303, 705, 726, 729, 732
Sau3AI GATC 5 cut(s) 42, 277, 323, 346, 619
Sau96I GGNCC 2 cut(s) 121, 591
SchI GAGTC 1 cut(s) 473
ScrFI CCNGG 1 cut(s) 741
SduI GDGCHC 3 cut(s) 55, 406, 417
SexAI ACCWGGT 1 cut(s) 739
SfcI CTRYAG 1 cut(s) 525
SfuI TTCGAA 1 cut(s) 180
SinI GGWCC 1 cut(s) 121
SmiMI CAYNNNNRTG 1 cut(s) 651
Sse9I AATT 3 cut(s) 186, 563, 658
SsiI CCGC 4 cut(s) 83, 86, 284, 522
SspMI CTAG 2 cut(s) 327, 746
StyD4I CCNGG 1 cut(s) 739
StyI CCWWGG 1 cut(s) 587
TaaI ACNGT 1 cut(s) 231
TaqI TCGA 2 cut(s) 180, 349
TasI AATT 3 cut(s) 186, 563, 658
TauI GCSGC 1 cut(s) 86
TscAI CASTG 2 cut(s) 111, 363
TseI GCWGC 8 cut(s) 32, 76, 146, 302, 704, 725, 728, 731
TspDTI ATGAA 5 cut(s) 215, 307, 355, 393, 641
TspRI CASTG 2 cut(s) 111, 363
VneI GTGCAC 1 cut(s) 413
VpaK11BI GGWCC 1 cut(s) 121
XapI RAATTY 1 cut(s) 658
XspI CTAG 2 cut(s) 327, 746
Zsp2I ATGCAT 1 cut(s) 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.