Rmu_sc0004886.1_g000009

TITAN-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004886.1
OG0007112
Physical Location & Seq
Reverse (-)
40633 .. 44185
3553 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1473 bp
atgaacaaccaattcatgcctcattcggcagcaaaaccacatgacatcgccgaggccaaggccagtctagctgtcagggttgccactggacgagaggttagcactggggtctctcacaacaatttacagcaagaagtagtccctacggtaatggaggagaaggagaaacgatcaaaggctcattcgtggaatcctaaccctaaccctaaatccaacgccacaacaatgaagaagaagcacgacttcgagtattgcacggtttgcaagctgaatcacgaccaaggtccgagccacaagtacatccccagccacaccaagtccctctctactttcctctctcgcttccaggccaagctctccgacgtcgttttcttcctcaaaaaccctaccttcctccgcctcgaacacgccgctcgcaaccgcctctggtgcgtcttctgcgatttcgacattgacgagcgggatagctccttcgcttgtggcaatgcaattaatcaccttgcgagtgccgaacatctgaagaatttgaagcacttcttgtggaaatacggtggaggaatggattctgtcgataaattcaggatattggaagctgaccttgctaagtgggagaagaagtgcaagtcattgaagagtgaagctatttctggaggaccaatgccaggaccttcaaatgatatccataataaacttgaatatggaattttagatacttttgaaaataatagtagttcaagtttttcaaatggtgttatgcctttactatatcatacaaatgagaatcaggtatcccattctggactttctcaagttgcaaatgttggctgctttgtgcaagatgttactgcgtcctcttctattaattccaaaggtttaacagttagtagccagggttctcttatttctaatggtaataaaggctcagctgacggcaatttcaatactgaaaggatgaacaaggtgtatcagcatggaagaatgacaataggacagagcagttctcaaggtgttcagaatctcactcaaattccttccaagggtccgaaagaggccagtggaaatgtgtatactggagcacttcctccttggtttgcagcagatgaagaaaatcagcttagtattccaatacaaccagtttctggtacccttcttcctccgtcaagtaagtcagggaagtccaagaaattaaacccaaaacgagtgggagcagcttgggcagaaaggagaaagagggagctggagatggaacaaaggggtgagattgtcaagagcgattatgatgctaattggctccccaattttggtggagtttggcaatctggtacccggaaagagactaaaaaagaatttgagttggagaaacttaatttagttaaggttgaaagtcaatctgagatgccaatcaagatacagccttacgtcagcaaaaggatggtgagttccatgttcccacttactgtgtctgtttgttga

Protein Analysis

490

Amino Acids

54.34

Weight (kDa)

9.14

Isoelectric Point (pI)

36.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCDC84 PF14968 84 - 454 2.1e-91 Coiled coil protein 84
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 366
Acc65I GGTACC 2 cut(s) 1142, 1322
AccB1I GGYRCC 2 cut(s) 1142, 1322
AccB7I CCANNNNNTGG 1 cut(s) 1139
AccBSI CCGCTC 2 cut(s) 413, 460
AccI GTMKAC 1 cut(s) 1067
AciI CCGC 4 cut(s) 397, 411, 421, 460
AcsI RAATTY 5 cut(s) 523, 575, 702, 1026, 1346
AcuI CTGAAG 1 cut(s) 539
AcyI GRCGYC 1 cut(s) 363
AfaI GTAC 3 cut(s) 299, 1144, 1324
AfiI CCNNNNNNNGG 4 cut(s) 662, 1037, 1139, 1301
AgsI TTSAA 9 cut(s) 529, 631, 672, 695, 719, 735, 744, 940, 1382
AjnI CCWGG 3 cut(s) 345, 661, 888
AloI GAACNNNNNNTCC 2 cut(s) 1423, 1455
Alw21I GWGCWC 1 cut(s) 1078
Alw26I GTCTC 2 cut(s) 115, 1328
AlwNI CAGNNNCTG 1 cut(s) 1139
AoxI GGCC 4 cut(s) 54, 60, 348, 1050
ApeKI GCWGC 4 cut(s) 29, 825, 1094, 1208
ApoI RAATTY 5 cut(s) 523, 575, 702, 1026, 1346
AseI ATTAAT 2 cut(s) 492, 861
Asp700I GAANNNNTTC 2 cut(s) 533, 562
Asp718I GGTACC 2 cut(s) 1142, 1322
AspS9I GGNCC 4 cut(s) 284, 653, 665, 1040
AsuC2I CCSGG 1 cut(s) 1327
AsuHPI GGTGA 3 cut(s) 488, 1268, 1447
AvaII GGWCC 4 cut(s) 284, 653, 665, 1040
BanI GGYRCC 2 cut(s) 1142, 1322
BbsI GAAGAC 1 cut(s) 427
Bbv12I GWGCWC 1 cut(s) 1078
BbvI GCAGC 4 cut(s) 41, 812, 1106, 1220
BccI CCATC 2 cut(s) 1237, 1426
BceAI ACGGC 1 cut(s) 946
BcgI CGANNNNNNTGC 2 cut(s) 1262, 1296
BciT130I CCWGG 3 cut(s) 347, 663, 890
BciVI GTATCC 1 cut(s) 799
BcnI CCSGG 1 cut(s) 1327
BcoDI GTCTC 2 cut(s) 115, 1328
BfaI CTAG 1 cut(s) 68
BfuI GTATCC 1 cut(s) 799
BisI GCNGC 5 cut(s) 30, 411, 826, 1095, 1209
BlpI GCTNAGC 1 cut(s) 922
BlsI GCNGC 5 cut(s) 31, 412, 827, 1096, 1210
Bme1390I CCNGG 4 cut(s) 347, 663, 890, 1327
Bme18I GGWCC 4 cut(s) 284, 653, 665, 1040
BmgT120I GGNCC 4 cut(s) 284, 653, 665, 1040
BmiI GGNNCC 4 cut(s) 1041, 1144, 1292, 1324
BmrFI CCNGG 4 cut(s) 347, 663, 890, 1327
BmrI ACTGGG 1 cut(s) 114
BmsI GCATC 2 cut(s) 1270, 1386
BmuI ACTGGG 1 cut(s) 114
BoxI GACNNNNGTC 1 cut(s) 282
BpiI GAAGAC 1 cut(s) 427
BplI GAGNNNNNCTC 2 cut(s) 985, 1017
BpmI CTGGAG 3 cut(s) 669, 1092, 1259
Bpu1102I GCTNAGC 1 cut(s) 922
BpuEI CTTGAG 2 cut(s) 792, 987
BpuMI CCSGG 1 cut(s) 1327
BsaBI GATNNNNATC 1 cut(s) 1400
BsaHI GRCGYC 1 cut(s) 363
BsaI GGTCTC 1 cut(s) 115
BsaJI CCNNGG 6 cut(s) 51, 57, 280, 889, 1035, 1085
BsaXI ACNNNNNCTCC 2 cut(s) 602, 632
Bsc4I CCNNNNNNNGG 4 cut(s) 662, 1037, 1139, 1301
Bse1I ACTGG 6 cut(s) 63, 91, 109, 1053, 1075, 1133
Bse3DI GCAATG 1 cut(s) 490
Bse8I GATNNNNATC 1 cut(s) 1400
BseBI CCWGG 3 cut(s) 347, 663, 890
BseDI CCNNGG 6 cut(s) 51, 57, 280, 889, 1035, 1085
BseGI GGATG 3 cut(s) 300, 957, 1437
BseJI GATNNNNATC 1 cut(s) 1400
BseLI CCNNNNNNNGG 4 cut(s) 662, 1037, 1139, 1301
BseMI GCAATG 1 cut(s) 490
BseMII CTCAG 2 cut(s) 936, 1383
BseNI ACTGG 6 cut(s) 63, 91, 109, 1053, 1075, 1133
BseRI GAGGAG 1 cut(s) 170
BseXI GCAGC 4 cut(s) 41, 812, 1106, 1220
BseYI CCCAGC 1 cut(s) 305
BshFI GGCC 4 cut(s) 56, 62, 350, 1052
BshNI GGYRCC 2 cut(s) 1142, 1322
BsiHKAI GWGCWC 1 cut(s) 1078
BsiSI CCGG 1 cut(s) 1327
BslFI GGGAC 2 cut(s) 125, 304
BslI CCNNNNNNNGG 4 cut(s) 662, 1037, 1139, 1301
BsmAI GTCTC 2 cut(s) 115, 1328
BsmFI GGGAC 2 cut(s) 125, 304
BsnI GGCC 4 cut(s) 56, 62, 350, 1052
Bso31I GGTCTC 1 cut(s) 115
Bsp1286I GDGCHC 1 cut(s) 1078
Bsp143I GATC 1 cut(s) 170
Bsp1720I GCTNAGC 1 cut(s) 922
BspACI CCGC 4 cut(s) 397, 411, 421, 460
BspANI GGCC 4 cut(s) 56, 62, 350, 1052
BspCNI CTCAG 2 cut(s) 935, 1384
BspLI GGNNCC 4 cut(s) 1041, 1144, 1292, 1324
BspT107I GGYRCC 2 cut(s) 1142, 1322
BspTNI GGTCTC 1 cut(s) 115
BsrBI CCGCTC 2 cut(s) 413, 460
BsrDI GCAATG 1 cut(s) 490
BsrI ACTGG 6 cut(s) 63, 91, 109, 1053, 1075, 1133
BssECI CCNNGG 6 cut(s) 51, 57, 280, 889, 1035, 1085
BssMI GATC 1 cut(s) 170
BssNAI GTATAC 1 cut(s) 1068
BssNI GRCGYC 1 cut(s) 363
BssT1I CCWWGG 4 cut(s) 57, 280, 1035, 1085
Bst1107I GTATAC 1 cut(s) 1068
Bst2UI CCWGG 3 cut(s) 347, 663, 890
Bst4CI ACNGT 5 cut(s) 148, 259, 551, 880, 1459
Bst6I CTCTTC 2 cut(s) 626, 859
BstACI GRCGYC 1 cut(s) 363
BstAPI GCANNNNNTGC 1 cut(s) 261
BstC8I GCNNGC 2 cut(s) 266, 415
BstDEI CTNAG 4 cut(s) 603, 922, 1115, 1392
BstF5I GGATG 3 cut(s) 300, 957, 1437
BstKTI GATC 1 cut(s) 173
BstMAI GTCTC 2 cut(s) 115, 1328
BstMBI GATC 1 cut(s) 170
BstMWI GCNNNNNNNGC 6 cut(s) 68, 261, 429, 438, 599, 1214
BstNI CCWGG 3 cut(s) 347, 663, 890
BstPAI GACNNNNGTC 1 cut(s) 282
BstSCI CCNGG 4 cut(s) 345, 661, 888, 1325
BstV1I GCAGC 4 cut(s) 41, 812, 1106, 1220
BstV2I GAAGAC 1 cut(s) 427
BstZ17I GTATAC 1 cut(s) 1068
BsuI GTATCC 1 cut(s) 799
BsuRI GGCC 4 cut(s) 56, 62, 350, 1052
BtgZI GCGATG 1 cut(s) 31
BtsCI GGATG 3 cut(s) 300, 957, 1437
BtsIMutI CAGTG 3 cut(s) 84, 102, 1060
Cac8I GCNNGC 2 cut(s) 266, 415
CaiI CAGNNNCTG 1 cut(s) 1139
Cfr13I GGNCC 4 cut(s) 284, 653, 665, 1040
CseI GACGC 2 cut(s) 421, 837
Csp6I GTAC 3 cut(s) 298, 1143, 1323
CspCI CAANNNNNGTGG 4 cut(s) 1182, 1217, 1285, 1320
CviAII CATG 4 cut(s) 16, 41, 971, 1444
CviQI GTAC 3 cut(s) 298, 1143, 1323
DdeI CTNAG 4 cut(s) 603, 922, 1115, 1392
DpnI GATC 1 cut(s) 172
DpnII GATC 1 cut(s) 170
Eam1104I CTCTTC 2 cut(s) 626, 859
EarI CTCTTC 2 cut(s) 626, 859
EciI GGCGGA 1 cut(s) 386
Eco130I CCWWGG 4 cut(s) 57, 280, 1035, 1085
Eco31I GGTCTC 1 cut(s) 115
Eco32I GATATC 1 cut(s) 679
Eco47I GGWCC 4 cut(s) 284, 653, 665, 1040
Eco57I CTGAAG 1 cut(s) 539
EcoO109I RGGNCCY 1 cut(s) 665
EcoRII CCWGG 3 cut(s) 345, 661, 888
EcoRV GATATC 1 cut(s) 679
EcoT14I CCWWGG 4 cut(s) 57, 280, 1035, 1085
ErhI CCWWGG 4 cut(s) 57, 280, 1035, 1085
FaeI CATG 4 cut(s) 19, 44, 974, 1447
FalI AAGNNNNNCTT 6 cut(s) 227, 259, 521, 553, 582, 614
FaqI GGGAC 2 cut(s) 125, 304
FatI CATG 4 cut(s) 15, 40, 970, 1443
FauI CCCGC 1 cut(s) 453
FblI GTMKAC 1 cut(s) 1067
Fnu4HI GCNGC 5 cut(s) 30, 411, 826, 1095, 1209
FokI GGATG 3 cut(s) 287, 964, 1444
Fsp4HI GCNGC 5 cut(s) 30, 411, 826, 1095, 1209
FspBI CTAG 1 cut(s) 68
GluI GCNGC 5 cut(s) 30, 411, 826, 1095, 1209
GsaI CCCAGC 1 cut(s) 309
GsuI CTGGAG 3 cut(s) 669, 1092, 1259
HaeIII GGCC 4 cut(s) 56, 62, 350, 1052
HapII CCGG 1 cut(s) 1327
HgaI GACGC 2 cut(s) 421, 837
Hin1I GRCGYC 1 cut(s) 363
Hin1II CATG 4 cut(s) 19, 44, 974, 1447
HinfI GANTC 5 cut(s) 190, 271, 563, 781, 1015
HpaII CCGG 1 cut(s) 1327
HphI GGTGA 3 cut(s) 488, 1268, 1447
Hpy166II GTNNAC 1 cut(s) 1068
Hpy188I TCNGA 6 cut(s) 288, 361, 519, 1014, 1044, 1393
Hpy188III TCNNGA 6 cut(s) 275, 580, 648, 798, 1267, 1405
Hpy8I GTNNAC 1 cut(s) 1068
Hpy99I CGWCG 2 cut(s) 365, 368
HpyAV CCTTC 6 cut(s) 154, 400, 481, 678, 1041, 1157
HpyCH4III ACNGT 5 cut(s) 148, 259, 551, 880, 1459
HpyCH4IV ACGT 2 cut(s) 363, 1419
HpyCH4V TGCA 7 cut(s) 255, 264, 488, 621, 815, 835, 1094
HpyF10VI GCNNNNNNNGC 6 cut(s) 68, 261, 429, 438, 599, 1214
HpyF3I CTNAG 4 cut(s) 603, 922, 1115, 1392
HpySE526I ACGT 2 cut(s) 363, 1419
Hsp92I GRCGYC 1 cut(s) 363
Hsp92II CATG 4 cut(s) 19, 44, 974, 1447
KpnI GGTACC 2 cut(s) 1146, 1326
Kzo9I GATC 1 cut(s) 170
LmnI GCTCC 5 cut(s) 473, 1073, 1205, 1234, 1296
Lsp1109I GCAGC 4 cut(s) 41, 812, 1106, 1220
LweI GCATC 2 cut(s) 1270, 1386
MaeI CTAG 1 cut(s) 68
MaeII ACGT 2 cut(s) 363, 1419
MaeIII GTNAC 1 cut(s) 841
MalI GATC 1 cut(s) 172
MbiI CCGCTC 2 cut(s) 413, 460
MboI GATC 1 cut(s) 170
MhlI GDGCHC 1 cut(s) 1078
MmeI TCCRAC 3 cut(s) 237, 384, 1335
MroXI GAANNNNTTC 2 cut(s) 533, 562
MseI TTAA 6 cut(s) 492, 861, 875, 1187, 1365, 1374
MslI CAYNNNNRTG 2 cut(s) 224, 774
MspA1I CMGCKG 1 cut(s) 926
MspI CCGG 1 cut(s) 1327
MspR9I CCNGG 4 cut(s) 347, 663, 890, 1327
MvaI CCWGG 3 cut(s) 347, 663, 890
MwoI GCNNNNNNNGC 6 cut(s) 68, 261, 429, 438, 599, 1214
NciI CCSGG 1 cut(s) 1327
NdeII GATC 1 cut(s) 170
NlaIII CATG 4 cut(s) 19, 44, 974, 1447
NlaIV GGNNCC 4 cut(s) 1041, 1144, 1292, 1324
NmeAIII GCCGAG 1 cut(s) 76
PcsI WCGNNNNNNNCGW 1 cut(s) 453
PdmI GAANNNNTTC 2 cut(s) 533, 562
PfeI GAWTC 5 cut(s) 190, 271, 563, 781, 1015
PflMI CCANNNNNTGG 1 cut(s) 1139
PkrI GCNGC 5 cut(s) 31, 412, 827, 1096, 1210
PpuMI RGGWCCY 1 cut(s) 665
PshAI GACNNNNGTC 1 cut(s) 282
PshBI ATTAAT 2 cut(s) 492, 861
Psp5II RGGWCCY 1 cut(s) 665
Psp6I CCWGG 3 cut(s) 345, 661, 888
PspFI CCCAGC 1 cut(s) 305
PspGI CCWGG 3 cut(s) 345, 661, 888
PspN4I GGNNCC 4 cut(s) 1041, 1144, 1292, 1324
PspPI GGNCC 4 cut(s) 284, 653, 665, 1040
PspPPI RGGWCCY 1 cut(s) 665
PsrI GAACNNNNNNTAC 2 cut(s) 877, 909
PstNI CAGNNNCTG 1 cut(s) 1139
PvuII CAGCTG 1 cut(s) 926
RsaI GTAC 3 cut(s) 299, 1144, 1324
RsaNI GTAC 3 cut(s) 298, 1143, 1323
RseI CAYNNNNRTG 2 cut(s) 224, 774
SaqAI TTAA 6 cut(s) 492, 861, 875, 1187, 1365, 1374
SatI GCNGC 5 cut(s) 30, 411, 826, 1095, 1209
Sau3AI GATC 1 cut(s) 170
Sau96I GGNCC 4 cut(s) 284, 653, 665, 1040
ScrFI CCNGG 4 cut(s) 347, 663, 890, 1327
SduI GDGCHC 1 cut(s) 1078
SfaNI GCATC 2 cut(s) 1270, 1386
SinI GGWCC 4 cut(s) 284, 653, 665, 1040
SmiMI CAYNNNNRTG 2 cut(s) 224, 774
SmlI CTYRAG 2 cut(s) 807, 1002
SmoI CTYRAG 2 cut(s) 807, 1002
SsiI CCGC 4 cut(s) 397, 411, 421, 460
SspMI CTAG 1 cut(s) 68
StyD4I CCNGG 4 cut(s) 345, 661, 888, 1325
StyI CCWWGG 4 cut(s) 57, 280, 1035, 1085
TaaI ACNGT 5 cut(s) 148, 259, 551, 880, 1459
TaiI ACGT 2 cut(s) 366, 1422
TaqI TCGA 4 cut(s) 246, 402, 447, 570
TatI WGTACW 1 cut(s) 297
TauI GCSGC 1 cut(s) 413
TfiI GAWTC 5 cut(s) 190, 271, 563, 781, 1015
Tru1I TTAA 6 cut(s) 492, 861, 875, 1187, 1365, 1374
Tru9I TTAA 6 cut(s) 492, 861, 875, 1187, 1365, 1374
TscAI CASTG 3 cut(s) 91, 109, 1060
TseI GCWGC 4 cut(s) 29, 825, 1094, 1208
TspDTI ATGAA 5 cut(s) 4, 17, 242, 968, 1116
TspGWI ACGGA 1 cut(s) 1146
TspRI CASTG 3 cut(s) 91, 109, 1060
Van91I CCANNNNNTGG 1 cut(s) 1139
VpaK11BI GGWCC 4 cut(s) 284, 653, 665, 1040
VspI ATTAAT 2 cut(s) 492, 861
XapI RAATTY 5 cut(s) 523, 575, 702, 1026, 1346
XmiI GTMKAC 1 cut(s) 1067
XmnI GAANNNNTTC 2 cut(s) 533, 562
XspI CTAG 1 cut(s) 68
ZraI GACGTC 1 cut(s) 364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.