Rmu_sc0005121.1_g000006

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005121.1
OG0004842
Physical Location & Seq
Reverse (-)
36903 .. 39647
2745 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1566 bp
atggctgacttctgccgaaatgggttcagttttcttgctctcaaaacctcatcattcatgcacagtagcggtgttcctatagatacgtatgctttgtgtagttctttaactgctgcatcttctattaaagatgttggcttcggtagaaagatacatgcccatgtggtaaagtcaggctggtgctccagtgtttttgttgggagtgctctgattgacttgtatgccaagtcattggttataggagatgcagaccaagtctttgatgaaatgccgctgagaaacactgtttgtgcaaacgcacttttatcaggttatggtgatgccaagttgtggacagaggcagtagaattgaccaggaagatgccggcactgagtttggagtatgatcacttgacattgtccgcggcattacgtgcctgtgctgggctagctgcagttgaattgggtaggcaggtacatggttatttgatgcgttcggtttatgatgtgggaagtgatatgtttctgctgagttccttgattgaaatgtatggtaaatgtgggctggtagccaaggctcagaaagttttaaattttgatggaaaaggagaggggaaaagagacgtggttatgtggacttcactgcttggtgtgtatggtagaaatggatactataaagaggtacttgaactattcaaagatatgatgatggaaggaattagcccagatggggtggcatttgtgacagtcatctcagcttgtgctcgcaccggtcaattgcggctcggcattgagtattttgaatccatggaactcaagttcagcttgacaccaggtccagaacactacagctgtctagttgatttgctgtgtagagctggtgaattaggcaaggcatggaagctcgttgatgagatgcttaataaaggtcacagcagctgcactgtccctatgtggggagctttgcttaatgcttgcaaagatcaaggtgatcttgaattgggtaaactggctgctcaaagggcacttgagttgaatcctcataatgatggtatttatgttttgctatcaaatttatatgccaggttcggggagcttcgagaccccgcagtggtctgcaacttcgcgccagaaaccctaaacgagagaacagctcccatgagcaatgattcatcttccgtaatgaacggtccagatcagcctgacacaagcgcgcccgcgcccaaaccacagcgcaccaagatcgttaaactcgaagacactgaaaagaagatttttcataagaaactcaaagatgttgagattagtgttccgatagtgtatggcaatattgcattctggctcggtaagaaggcaagcgagtaccaatcacataagtggactgtgtatgttcgtggggcaaccaatgaggatctgggagtggtgataaagcgtgttgttttccagttgcattccagtttcaataatcccacaagggtggttgagtcaccgccatttgagttatctgaatgtggctggggtgaatttgaaatcgcaattacactatttttccacagtgatgtttgcgagaagccattaaacttgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

521

Amino Acids

57.33

Weight (kDa)

5.99

Isoelectric Point (pI)

35.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 200 - 249 2.3e-10 PPR repeat family
PPR PF01535 203 - 233 3e-07 PPR repeat
PPR_1 PF12854 275 - 299 1.6e-06 PPR repeat
E_motif PF20431 324 - 362 1.4e-09 E motif
YEATS PF03366 449 - 519 2.4e-26 YEATS family
pYEATS PF20305 452 - 511 2.3e-10 prokaryotic YEATS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 442
AccB7I CCANNNNNTGG 1 cut(s) 330
AccII CGCG 4 cut(s) 404, 1106, 1193, 1199
AciI CCGC 8 cut(s) 69, 272, 402, 404, 760, 1086, 1197, 1469
AclWI GGATC 1 cut(s) 1398
AcsI RAATTY 3 cut(s) 571, 1051, 1502
AfaI GTAC 3 cut(s) 456, 663, 1343
AfiI CCNNNNNNNGG 7 cut(s) 330, 423, 709, 934, 935, 1068, 1090
AgeI ACCGGT 1 cut(s) 749
AgsI TTSAA 9 cut(s) 440, 524, 668, 676, 782, 977, 1015, 1441, 1508
AhdI GACNNNNNGTC 1 cut(s) 813
AjiI CACGTC 1 cut(s) 604
AjnI CCWGG 3 cut(s) 353, 811, 1061
AleI CACNNNNGTG 2 cut(s) 1354, 1454
AloI GAACNNNNNNTCC 2 cut(s) 782, 814
Alw21I GWGCWC 3 cut(s) 185, 208, 745
Alw26I GTCTC 2 cut(s) 594, 1074
AlwI GGATC 1 cut(s) 1398
AlwNI CAGNNNCTG 1 cut(s) 918
ApeKI GCWGC 5 cut(s) 113, 431, 915, 918, 992
ApoI RAATTY 3 cut(s) 571, 1051, 1502
ArsI GACNNNNNNTTYG 2 cut(s) 242, 274
AsiGI ACCGGT 1 cut(s) 749
AspLEI GCGC 5 cut(s) 1108, 1193, 1195, 1201, 1215
AspS9I GGNCC 2 cut(s) 815, 1169
AsuHPI GGTGA 6 cut(s) 329, 872, 980, 1414, 1458, 1511
AsuNHI GCTAGC 1 cut(s) 427
AvaII GGWCC 2 cut(s) 815, 1169
BaeGI GKGCMC 1 cut(s) 1006
BbsI GAAGAC 1 cut(s) 1242
Bbv12I GWGCWC 3 cut(s) 185, 208, 745
BbvI GCAGC 5 cut(s) 100, 418, 905, 927, 979
BccI CCATC 4 cut(s) 572, 682, 701, 1022
BcgI CGANNNNNNTGC 2 cut(s) 1204, 1238
BciT130I CCWGG 3 cut(s) 355, 813, 1063
BciVI GTATCC 1 cut(s) 641
BclI TGATCA 1 cut(s) 385
BcoDI GTCTC 2 cut(s) 594, 1074
BfaI CTAG 2 cut(s) 428, 836
BfmI CTRYAG 3 cut(s) 78, 432, 826
BfuAI ACCTGC 1 cut(s) 442
BfuI GTATCC 1 cut(s) 641
BisI GCNGC 8 cut(s) 114, 272, 405, 432, 761, 916, 919, 993
BlsI GCNGC 8 cut(s) 115, 273, 406, 433, 762, 917, 920, 994
Bme1390I CCNGG 3 cut(s) 355, 813, 1063
Bme18I GGWCC 2 cut(s) 815, 1169
BmeRI GACNNNNNGTC 1 cut(s) 813
BmgBI CACGTC 1 cut(s) 604
BmgT120I GGNCC 2 cut(s) 815, 1169
BmrFI CCNGG 3 cut(s) 355, 813, 1063
BmsI GCATC 6 cut(s) 125, 235, 310, 351, 459, 885
BmtI GCTAGC 1 cut(s) 431
BpiI GAAGAC 1 cut(s) 1242
BplI GAGNNNNNCTC 2 cut(s) 1117, 1149
BpmI CTGGAG 1 cut(s) 169
BpuEI CTTGAG 2 cut(s) 779, 1028
BsaAI YACGTR 2 cut(s) 87, 413
BsaI GGTCTC 1 cut(s) 1074
BsaJI CCNNGG 3 cut(s) 402, 552, 786
BsaWI WCCGGW 1 cut(s) 749
Bsc4I CCNNNNNNNGG 7 cut(s) 330, 423, 709, 934, 935, 1068, 1090
Bse118I RCCGGY 2 cut(s) 364, 749
Bse1I ACTGG 4 cut(s) 186, 993, 1423, 1434
Bse3DI GCAATG 1 cut(s) 1150
BseBI CCWGG 3 cut(s) 355, 813, 1063
BseDI CCNNGG 3 cut(s) 402, 552, 786
BseLI CCNNNNNNNGG 7 cut(s) 330, 423, 709, 934, 935, 1068, 1090
BseMI GCAATG 1 cut(s) 1150
BseMII CTCAG 5 cut(s) 266, 362, 500, 572, 747
BseNI ACTGG 4 cut(s) 186, 993, 1423, 1434
BsePI GCGCGC 1 cut(s) 1191
BseSI GKGCMC 1 cut(s) 1006
BseXI GCAGC 5 cut(s) 100, 418, 905, 927, 979
BseYI CCCAGC 2 cut(s) 422, 1494
BsgI GTGCAG 1 cut(s) 904
Bsh1236I CGCG 4 cut(s) 404, 1106, 1193, 1199
BshTI ACCGGT 1 cut(s) 749
BsiHKAI GWGCWC 3 cut(s) 185, 208, 745
BsiSI CCGG 2 cut(s) 365, 750
BslFI GGGAC 1 cut(s) 911
BslI CCNNNNNNNGG 7 cut(s) 330, 423, 709, 934, 935, 1068, 1090
BsmAI GTCTC 2 cut(s) 594, 1074
BsmBI CGTCTC 1 cut(s) 594
BsmFI GGGAC 1 cut(s) 911
BsmI GAATGC 2 cut(s) 1313, 1429
Bso31I GGTCTC 1 cut(s) 1074
Bsp1286I GDGCHC 4 cut(s) 185, 208, 745, 1006
Bsp143I GATC 6 cut(s) 385, 961, 970, 1174, 1221, 1390
Bsp19I CCATGG 1 cut(s) 786
BspACI CCGC 8 cut(s) 69, 272, 402, 404, 760, 1086, 1197, 1469
BspCNI CTCAG 5 cut(s) 267, 363, 501, 571, 746
BspFNI CGCG 4 cut(s) 404, 1106, 1193, 1199
BspMAI CTGCAG 1 cut(s) 436
BspMI ACCTGC 1 cut(s) 442
BspOI GCTAGC 1 cut(s) 431
BspPI GGATC 1 cut(s) 1398
BspTNI GGTCTC 1 cut(s) 1074
BsrDI GCAATG 1 cut(s) 1150
BsrFI RCCGGY 2 cut(s) 364, 749
BsrI ACTGG 4 cut(s) 186, 993, 1423, 1434
BssAI RCCGGY 2 cut(s) 364, 749
BssECI CCNNGG 3 cut(s) 402, 552, 786
BssHII GCGCGC 1 cut(s) 1191
BssMI GATC 6 cut(s) 385, 961, 970, 1174, 1221, 1390
BssT1I CCWWGG 2 cut(s) 552, 786
Bst2UI CCWGG 3 cut(s) 355, 813, 1063
Bst4CI ACNGT 7 cut(s) 65, 286, 727, 925, 1169, 1363, 1535
BstAPI GCANNNNNTGC 1 cut(s) 413
BstBAI YACGTR 2 cut(s) 87, 413
BstC8I GCNNGC 7 cut(s) 366, 429, 745, 955, 1193, 1197, 1336
BstDEI CTNAG 5 cut(s) 275, 371, 509, 558, 733
BstDSI CCRYGG 2 cut(s) 402, 786
BstFNI CGCG 4 cut(s) 404, 1106, 1193, 1199
BstHHI GCGC 5 cut(s) 1108, 1193, 1195, 1201, 1215
BstKTI GATC 6 cut(s) 388, 964, 973, 1177, 1224, 1393
BstMAI GTCTC 2 cut(s) 594, 1074
BstMBI GATC 6 cut(s) 385, 961, 970, 1174, 1221, 1390
BstMWI GCNNNNNNNGC 3 cut(s) 413, 428, 1001
BstNI CCWGG 3 cut(s) 355, 813, 1063
BstNSI RCATGY 1 cut(s) 158
BstSCI CCNGG 3 cut(s) 353, 811, 1061
BstSFI CTRYAG 3 cut(s) 78, 432, 826
BstSLI GKGCMC 1 cut(s) 1006
BstSNI TACGTA 1 cut(s) 87
BstUI CGCG 4 cut(s) 404, 1106, 1193, 1199
BstV1I GCAGC 5 cut(s) 100, 418, 905, 927, 979
BstV2I GAAGAC 1 cut(s) 1242
BstX2I RGATCY 1 cut(s) 1390
BstXI CCANNNNNNTGG 2 cut(s) 232, 1456
BstYI RGATCY 1 cut(s) 1390
BsuI GTATCC 1 cut(s) 641
BtgI CCRYGG 2 cut(s) 402, 786
BtrI CACGTC 1 cut(s) 604
BtsI GCAGTG 2 cut(s) 620, 1095
BtsIMutI CAGTG 8 cut(s) 193, 282, 368, 620, 921, 1095, 1239, 1540
BveI ACCTGC 1 cut(s) 442
Cac8I GCNNGC 7 cut(s) 366, 429, 745, 955, 1193, 1197, 1336
CaiI CAGNNNCTG 1 cut(s) 918
CfoI GCGC 5 cut(s) 1108, 1193, 1195, 1201, 1215
Cfr10I RCCGGY 2 cut(s) 364, 749
Cfr13I GGNCC 2 cut(s) 815, 1169
Cfr42I CCGCGG 1 cut(s) 405
CsiI ACCWGGT 1 cut(s) 811
Csp6I GTAC 3 cut(s) 455, 662, 1342
CspAI ACCGGT 1 cut(s) 749
CviAII CATG 7 cut(s) 58, 155, 161, 458, 787, 876, 1138
CviQI GTAC 3 cut(s) 455, 662, 1342
DdeI CTNAG 5 cut(s) 275, 371, 509, 558, 733
DpnI GATC 6 cut(s) 387, 963, 972, 1176, 1223, 1392
DpnII GATC 6 cut(s) 385, 961, 970, 1174, 1221, 1390
DraI TTTAAA 1 cut(s) 570
DriI GACNNNNNGTC 1 cut(s) 813
Eam1105I GACNNNNNGTC 1 cut(s) 813
Eco105I TACGTA 1 cut(s) 87
Eco130I CCWWGG 2 cut(s) 552, 786
Eco31I GGTCTC 1 cut(s) 1074
Eco47I GGWCC 2 cut(s) 815, 1169
EcoRII CCWGG 3 cut(s) 353, 811, 1061
EcoT14I CCWWGG 2 cut(s) 552, 786
ErhI CCWWGG 2 cut(s) 552, 786
Esp3I CGTCTC 1 cut(s) 594
FaeI CATG 7 cut(s) 61, 158, 164, 461, 790, 879, 1141
FalI AAGNNNNNCTT 6 cut(s) 648, 680, 788, 820, 957, 989
FaqI GGGAC 1 cut(s) 911
FatI CATG 7 cut(s) 57, 154, 160, 457, 786, 875, 1137
FauI CCCGC 2 cut(s) 1093, 1204
FbaI TGATCA 1 cut(s) 385
Fnu4HI GCNGC 8 cut(s) 114, 272, 405, 432, 761, 916, 919, 993
Fsp4HI GCNGC 8 cut(s) 114, 272, 405, 432, 761, 916, 919, 993
FspBI CTAG 2 cut(s) 428, 836
GlaI GCGC 5 cut(s) 1107, 1192, 1194, 1200, 1214
GluI GCNGC 8 cut(s) 114, 272, 405, 432, 761, 916, 919, 993
GsaI CCCAGC 2 cut(s) 426, 1498
GsuI CTGGAG 1 cut(s) 169
HapII CCGG 2 cut(s) 365, 750
HhaI GCGC 5 cut(s) 1108, 1193, 1195, 1201, 1215
Hin1II CATG 7 cut(s) 61, 158, 164, 461, 790, 879, 1141
Hin6I GCGC 5 cut(s) 1106, 1191, 1193, 1199, 1213
HinP1I GCGC 5 cut(s) 1106, 1191, 1193, 1199, 1213
HinfI GANTC 4 cut(s) 782, 1015, 1148, 1463
HpaII CCGG 2 cut(s) 365, 750
HphI GGTGA 6 cut(s) 329, 872, 980, 1414, 1458, 1511
Hpy166II GTNNAC 4 cut(s) 333, 615, 986, 1359
Hpy188I TCNGA 4 cut(s) 210, 561, 1293, 1486
Hpy188III TCNNGA 4 cut(s) 818, 974, 1079, 1172
Hpy8I GTNNAC 4 cut(s) 333, 615, 986, 1359
HpyAV CCTTC 2 cut(s) 686, 1324
HpyCH4III ACNGT 7 cut(s) 65, 286, 727, 925, 1169, 1363, 1535
HpyCH4IV ACGT 3 cut(s) 86, 412, 603
HpyF10VI GCNNNNNNNGC 3 cut(s) 413, 428, 1001
HpyF3I CTNAG 5 cut(s) 275, 371, 509, 558, 733
HpySE526I ACGT 3 cut(s) 86, 412, 603
Hsp92II CATG 7 cut(s) 61, 158, 164, 461, 790, 879, 1141
HspAI GCGC 5 cut(s) 1106, 1191, 1193, 1199, 1213
KroI GCCGGC 1 cut(s) 364
KroNI GCCGGC 1 cut(s) 366
Ksp22I TGATCA 1 cut(s) 385
KspI CCGCGG 1 cut(s) 405
Kzo9I GATC 6 cut(s) 385, 961, 970, 1174, 1221, 1390
LmnI GCTCC 4 cut(s) 188, 938, 1072, 1138
Lsp1109I GCAGC 5 cut(s) 100, 418, 905, 927, 979
LweI GCATC 6 cut(s) 125, 235, 310, 351, 459, 885
MabI ACCWGGT 1 cut(s) 811
MaeI CTAG 2 cut(s) 428, 836
MaeII ACGT 3 cut(s) 86, 412, 603
MaeIII GTNAC 3 cut(s) 721, 908, 1464
MalI GATC 6 cut(s) 387, 963, 972, 1176, 1223, 1392
MboI GATC 6 cut(s) 385, 961, 970, 1174, 1221, 1390
MboII GAAGA 5 cut(s) 111, 370, 1146, 1247, 1261
MfeI CAATTG 1 cut(s) 755
MflI RGATCY 1 cut(s) 1390
MhlI GDGCHC 4 cut(s) 185, 208, 745, 1006
MlyI GAGTC 1 cut(s) 1472
MnlI CCTC 6 cut(s) 58, 331, 583, 652, 1029, 1381
MroNI GCCGGC 1 cut(s) 364
MseI TTAA 7 cut(s) 107, 126, 569, 900, 948, 1227, 1556
MslI CAYNNNNRTG 5 cut(s) 159, 1026, 1354, 1454, 1536
MspA1I CMGCKG 4 cut(s) 274, 404, 831, 918
MspI CCGG 2 cut(s) 365, 750
MspR9I CCNGG 3 cut(s) 355, 813, 1063
MunI CAATTG 1 cut(s) 755
Mva1269I GAATGC 2 cut(s) 1313, 1429
MvaI CCWGG 3 cut(s) 355, 813, 1063
MvnI CGCG 4 cut(s) 404, 1106, 1193, 1199
MwoI GCNNNNNNNGC 3 cut(s) 413, 428, 1001
NaeI GCCGGC 1 cut(s) 366
NcoI CCATGG 1 cut(s) 786
NdeII GATC 6 cut(s) 385, 961, 970, 1174, 1221, 1390
NgoMIV GCCGGC 1 cut(s) 364
NheI GCTAGC 1 cut(s) 427
NlaIII CATG 7 cut(s) 61, 158, 164, 461, 790, 879, 1141
NmeAIII GCCGAG 1 cut(s) 744
NmuCI GTSAC 3 cut(s) 721, 908, 1464
NspI RCATGY 1 cut(s) 158
OliI CACNNNNGTG 2 cut(s) 1354, 1454
PauI GCGCGC 1 cut(s) 1191
PcsI WCGNNNNNNNCGW 1 cut(s) 1230
PctI GAATGC 2 cut(s) 1313, 1429
PdiI GCCGGC 1 cut(s) 366
PfeI GAWTC 3 cut(s) 782, 1015, 1148
PflFI GACNNNGTC 2 cut(s) 254, 397
PflMI CCANNNNNTGG 1 cut(s) 330
PinAI ACCGGT 1 cut(s) 749
PkrI GCNGC 8 cut(s) 115, 273, 406, 433, 762, 917, 920, 994
PleI GAGTC 1 cut(s) 1471
PpsI GAGTC 1 cut(s) 1471
Ppu21I YACGTR 2 cut(s) 87, 413
Psp6I CCWGG 3 cut(s) 353, 811, 1061
PspFI CCCAGC 2 cut(s) 422, 1494
PspGI CCWGG 3 cut(s) 353, 811, 1061
PspPI GGNCC 2 cut(s) 815, 1169
PstI CTGCAG 1 cut(s) 436
PstNI CAGNNNCTG 1 cut(s) 918
PsuI RGATCY 1 cut(s) 1390
PsyI GACNNNGTC 2 cut(s) 254, 397
PteI GCGCGC 1 cut(s) 1191
PvuII CAGCTG 2 cut(s) 831, 918
RsaI GTAC 3 cut(s) 456, 663, 1343
RsaNI GTAC 3 cut(s) 455, 662, 1342
RseI CAYNNNNRTG 5 cut(s) 159, 1026, 1354, 1454, 1536
SacII CCGCGG 1 cut(s) 405
SaqAI TTAA 7 cut(s) 107, 126, 569, 900, 948, 1227, 1556
SatI GCNGC 8 cut(s) 114, 272, 405, 432, 761, 916, 919, 993
Sau3AI GATC 6 cut(s) 385, 961, 970, 1174, 1221, 1390
Sau96I GGNCC 2 cut(s) 815, 1169
SchI GAGTC 1 cut(s) 1472
ScrFI CCNGG 3 cut(s) 355, 813, 1063
SduI GDGCHC 4 cut(s) 185, 208, 745, 1006
SexAI ACCWGGT 1 cut(s) 811
SfaNI GCATC 6 cut(s) 125, 235, 310, 351, 459, 885
SfcI CTRYAG 3 cut(s) 78, 432, 826
Sfr303I CCGCGG 1 cut(s) 405
SgrBI CCGCGG 1 cut(s) 405
SinI GGWCC 2 cut(s) 815, 1169
SmiMI CAYNNNNRTG 5 cut(s) 159, 1026, 1354, 1454, 1536
SmlI CTYRAG 2 cut(s) 794, 1007
SmoI CTYRAG 2 cut(s) 794, 1007
SnaBI TACGTA 1 cut(s) 87
SsiI CCGC 8 cut(s) 69, 272, 402, 404, 760, 1086, 1197, 1469
SspI AATATT 1 cut(s) 1309
SspMI CTAG 2 cut(s) 428, 836
StyD4I CCNGG 3 cut(s) 353, 811, 1061
StyI CCWWGG 2 cut(s) 552, 786
TaaI ACNGT 7 cut(s) 65, 286, 727, 925, 1169, 1363, 1535
TaiI ACGT 3 cut(s) 89, 415, 606
TaqI TCGA 2 cut(s) 1078, 1233
TauI GCSGC 3 cut(s) 274, 407, 763
TfiI GAWTC 3 cut(s) 782, 1015, 1148
Tru1I TTAA 7 cut(s) 107, 126, 569, 900, 948, 1227, 1556
Tru9I TTAA 7 cut(s) 107, 126, 569, 900, 948, 1227, 1556
TscAI CASTG 8 cut(s) 193, 289, 375, 627, 928, 1095, 1246, 1540
TseFI GTSAC 3 cut(s) 721, 908, 1464
TseI GCWGC 5 cut(s) 113, 431, 915, 918, 992
Tsp45I GTSAC 3 cut(s) 721, 908, 1464
TspDTI ATGAA 5 cut(s) 46, 279, 1140, 1178, 1247
TspGWI ACGGA 1 cut(s) 1147
TspRI CASTG 8 cut(s) 193, 289, 375, 627, 928, 1095, 1246, 1540
Tth111I GACNNNGTC 2 cut(s) 254, 397
Van91I CCANNNNNTGG 1 cut(s) 330
VpaK11BI GGWCC 2 cut(s) 815, 1169
XapI RAATTY 3 cut(s) 571, 1051, 1502
XceI RCATGY 1 cut(s) 158
XcmI CCANNNNNNNNNTGG 1 cut(s) 1390
XspI CTAG 2 cut(s) 428, 836
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.