Rmu_sc0005279.1_g000001

tRNA nucleoside ribose methylation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005279.1
OG0005805
Physical Location & Seq
Forward (+)
9635 .. 12220
2586 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1143 bp
atgaaaaccccctttgcctccacaactcaccatactggcatactccactctcttgcttcgcgccaaaattctccaatggcgacctgcaaaaccctagtccacacttctctctcttcaatcggctccactctccgaccccaattcattcctctcctcaatctccgcttctcccctctcctttgccccaacaaagggctcagagtttccaatgtcacttctcggccgtacgcttcaattgcagcagcagaagccgcggacttcacagacgaatctgctccgtcgccagccgaaaccctagatccatccgagtcgcaagacaccgtggagcacctcctcacgcaccgagacgacgtcgtgaagtttatgaaaatggaacgccgccctctacggtccccactcggaggcgataggtggttcccgtacctcgacagattcaactgcggcggtgcttacctcagcagcggtgagatactggaagcggttgatccgtacattatggatgtgaggaaggagaggtttaggaatgtggtgaggaatcggagctactcggtgtgcttggtggtggagggattgggagatttcggaaatgtatcggcggtgttccggtccgccgacgcgctcgggattcagtcggttcatgtgctctcttgtgacgcatccaaaaggtacagagaaaatcgccatgtcagcatgggagcagagaaatggttggatattgaactttgggactccactcaagagtgctttgaggttctaacatcgcgtggttatcgaattgcaacaacacatgtggggatggatgcggtgtctatttacaacatggactggtcctgcccaactgcaatagtagtcggaaatgagaataggggaataagtgatgaggccctggatttgtcagatttgcattgcagtattccaatgaagggcatggtggattctttcaatgtttcagttgctgcaggcattctcatgcaccatgctgtttgtgatagaacttctcgcctgggctgtcatggtgatctgacatctgaagagaaccaaattcttcttgctgagttttctctgcgtcatagcaagaattcaattagcattgctcatgagtatgcaaagcggaaggcagctctgctcacaccaaggctttga

Protein Analysis

380

Amino Acids

42.09

Weight (kDa)

6.43

Isoelectric Point (pI)

47.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SpoU_methylase PF00588 184 - 325 1.8e-35 SpoU rRNA Methylase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 354
Acc36I ACCTGC 1 cut(s) 92
AccII CGCG 4 cut(s) 61, 254, 617, 763
AclWI GGATC 2 cut(s) 293, 479
AcoI YGGCCR 1 cut(s) 221
AcsI RAATTY 3 cut(s) 67, 1041, 1078
AcuI CTGAAG 1 cut(s) 1050
AcyI GRCGYC 1 cut(s) 351
AfaI GTAC 4 cut(s) 227, 422, 491, 668
AfiI CCNNNNNNNGG 4 cut(s) 191, 192, 401, 923
AflIII ACRYGT 1 cut(s) 787
AgsI TTSAA 6 cut(s) 117, 234, 436, 719, 943, 1083
AjnI CCWGG 2 cut(s) 885, 1002
AluBI AGCT 2 cut(s) 543, 1121
AluI AGCT 2 cut(s) 543, 1121
Alw21I GWGCWC 2 cut(s) 330, 645
Alw26I GTCTC 1 cut(s) 339
AlwI GGATC 2 cut(s) 293, 479
AlwNI CAGNNNCTG 1 cut(s) 956
Ama87I CYCGRG 1 cut(s) 620
AoxI GGCC 2 cut(s) 221, 882
ApeKI GCWGC 5 cut(s) 239, 242, 459, 956, 1118
ApoI RAATTY 3 cut(s) 67, 1041, 1078
ArsI GACNNNNNNTTYG 2 cut(s) 81, 113
AspLEI GCGC 2 cut(s) 63, 619
AspS9I GGNCC 4 cut(s) 390, 606, 828, 883
AsuHPI GGTGA 4 cut(s) 20, 476, 541, 1028
AvaI CYCGRG 1 cut(s) 620
AvaII GGWCC 3 cut(s) 390, 606, 828
BanII GRGCYC 1 cut(s) 198
Bbv12I GWGCWC 2 cut(s) 330, 645
BbvCI CCTCAGC 1 cut(s) 455
BbvI GCAGC 5 cut(s) 251, 254, 471, 943, 1130
BccI CCATC 2 cut(s) 310, 790
BceAI ACGGC 1 cut(s) 208
BciT130I CCWGG 2 cut(s) 887, 1004
BcoDI GTCTC 1 cut(s) 339
BfaI CTAG 2 cut(s) 95, 296
BfmI CTRYAG 1 cut(s) 957
BfuAI ACCTGC 1 cut(s) 92
BisI GCNGC 8 cut(s) 240, 243, 252, 379, 442, 460, 957, 1119
BlsI GCNGC 8 cut(s) 241, 244, 253, 380, 443, 461, 958, 1120
Bme1390I CCNGG 2 cut(s) 887, 1004
Bme18I GGWCC 3 cut(s) 390, 606, 828
BmeT110I CYCGRG 1 cut(s) 620
BmgT120I GGNCC 4 cut(s) 390, 606, 828, 883
BmiI GGNNCC 3 cut(s) 124, 392, 416
BmrFI CCNGG 2 cut(s) 887, 1004
BmsI GCATC 2 cut(s) 665, 790
Bpu10I CCTNAGC 1 cut(s) 455
BpuEI CTTGAG 1 cut(s) 720
BsaHI GRCGYC 1 cut(s) 351
BsaJI CCNNGG 5 cut(s) 252, 321, 885, 1003, 1133
BsaWI WCCGGW 1 cut(s) 603
Bsc4I CCNNNNNNNGG 4 cut(s) 191, 192, 401, 923
Bse1I ACTGG 3 cut(s) 40, 477, 830
Bse3DI GCAATG 2 cut(s) 904, 1089
BseBI CCWGG 2 cut(s) 887, 1004
BseDI CCNNGG 5 cut(s) 252, 321, 885, 1003, 1133
BseGI GGATG 5 cut(s) 302, 505, 656, 801, 805
BseLI CCNNNNNNNGG 4 cut(s) 191, 192, 401, 923
BseMI GCAATG 2 cut(s) 904, 1089
BseMII CTCAG 3 cut(s) 211, 469, 1044
BseNI ACTGG 3 cut(s) 40, 477, 830
BseRI GAGGAG 2 cut(s) 143, 323
BseX3I CGGCCG 1 cut(s) 221
BseXI GCAGC 5 cut(s) 251, 254, 471, 943, 1130
Bsh1236I CGCG 4 cut(s) 61, 254, 617, 763
Bsh1285I CGRYCG 1 cut(s) 224
BshFI GGCC 2 cut(s) 223, 884
BsiEI CGRYCG 1 cut(s) 224
BsiHKAI GWGCWC 2 cut(s) 330, 645
BsiHKCI CYCGRG 1 cut(s) 620
BsiSI CCGG 1 cut(s) 604
BsiWI CGTACG 1 cut(s) 225
BslFI GGGAC 2 cut(s) 376, 740
BslI CCNNNNNNNGG 4 cut(s) 191, 192, 401, 923
BsmAI GTCTC 1 cut(s) 339
BsmBI CGTCTC 1 cut(s) 339
BsmFI GGGAC 2 cut(s) 376, 740
BsmI GAATGC 1 cut(s) 963
BsnI GGCC 2 cut(s) 223, 884
BsoBI CYCGRG 1 cut(s) 620
Bsp1286I GDGCHC 3 cut(s) 198, 330, 645
Bsp143I GATC 3 cut(s) 298, 484, 1018
BspANI GGCC 2 cut(s) 223, 884
BspCNI CTCAG 3 cut(s) 210, 468, 1045
BspFNI CGCG 4 cut(s) 61, 254, 617, 763
BspHI TCATGA 1 cut(s) 1096
BspLI GGNNCC 3 cut(s) 124, 392, 416
BspMAI CTGCAG 1 cut(s) 961
BspMI ACCTGC 1 cut(s) 92
BspPI GGATC 2 cut(s) 293, 479
BsrDI GCAATG 2 cut(s) 904, 1089
BsrI ACTGG 3 cut(s) 40, 477, 830
BssECI CCNNGG 5 cut(s) 252, 321, 885, 1003, 1133
BssMI GATC 3 cut(s) 298, 484, 1018
BssNI GRCGYC 1 cut(s) 351
BssT1I CCWWGG 1 cut(s) 1133
Bst2UI CCWGG 2 cut(s) 887, 1004
Bst4CI ACNGT 2 cut(s) 322, 390
Bst6I CTCTTC 2 cut(s) 118, 1026
BstACI GRCGYC 1 cut(s) 351
BstC8I GCNNGC 2 cut(s) 285, 961
BstDEI CTNAG 3 cut(s) 197, 455, 1053
BstDSI CCRYGG 2 cut(s) 252, 321
BstF5I GGATG 5 cut(s) 302, 505, 656, 801, 805
BstFNI CGCG 4 cut(s) 61, 254, 617, 763
BstHHI GCGC 2 cut(s) 63, 619
BstKTI GATC 3 cut(s) 301, 487, 1021
BstMAI GTCTC 1 cut(s) 339
BstMBI GATC 3 cut(s) 298, 484, 1018
BstMCI CGRYCG 1 cut(s) 224
BstMWI GCNNNNNNNGC 4 cut(s) 236, 248, 251, 687
BstNI CCWGG 2 cut(s) 887, 1004
BstNSI RCATGY 1 cut(s) 791
BstSCI CCNGG 2 cut(s) 885, 1002
BstSFI CTRYAG 1 cut(s) 957
BstUI CGCG 4 cut(s) 61, 254, 617, 763
BstV1I GCAGC 5 cut(s) 251, 254, 471, 943, 1130
BstX2I RGATCY 1 cut(s) 298
BstYI RGATCY 1 cut(s) 298
BstZI CGGCCG 1 cut(s) 221
BsuRI GGCC 2 cut(s) 223, 884
BtgI CCRYGG 2 cut(s) 252, 321
BtgZI GCGATG 1 cut(s) 744
BtsCI GGATG 5 cut(s) 302, 505, 656, 801, 805
BveI ACCTGC 1 cut(s) 92
Cac8I GCNNGC 2 cut(s) 285, 961
CaiI CAGNNNCTG 1 cut(s) 956
CciI TCATGA 1 cut(s) 1096
CfoI GCGC 2 cut(s) 63, 619
Cfr13I GGNCC 4 cut(s) 390, 606, 828, 883
Cfr42I CCGCGG 1 cut(s) 255
CpoI CGGWCCG 1 cut(s) 606
CseI GACGC 3 cut(s) 623, 662, 1055
Csp6I GTAC 4 cut(s) 226, 421, 490, 667
CspCI CAANNNNNGTGG 2 cut(s) 771, 806
CspI CGGWCCG 1 cut(s) 606
CviQI GTAC 4 cut(s) 226, 421, 490, 667
DdeI CTNAG 3 cut(s) 197, 455, 1053
DpnI GATC 3 cut(s) 300, 486, 1020
DpnII GATC 3 cut(s) 298, 484, 1018
EaeI YGGCCR 1 cut(s) 221
EagI CGGCCG 1 cut(s) 221
Eam1104I CTCTTC 2 cut(s) 118, 1026
EarI CTCTTC 2 cut(s) 118, 1026
EciI GGCGGA 1 cut(s) 598
EclXI CGGCCG 1 cut(s) 221
Eco130I CCWWGG 1 cut(s) 1133
Eco24I GRGCYC 1 cut(s) 198
Eco47I GGWCC 3 cut(s) 390, 606, 828
Eco52I CGGCCG 1 cut(s) 221
Eco57I CTGAAG 1 cut(s) 1050
Eco88I CYCGRG 1 cut(s) 620
EcoO109I RGGNCCY 1 cut(s) 883
EcoRI GAATTC 1 cut(s) 1078
EcoRII CCWGG 2 cut(s) 885, 1002
EcoT14I CCWWGG 1 cut(s) 1133
EcoT38I GRGCYC 1 cut(s) 198
ErhI CCWWGG 1 cut(s) 1133
Esp3I CGTCTC 1 cut(s) 339
FaqI GGGAC 2 cut(s) 376, 740
Fnu4HI GCNGC 8 cut(s) 240, 243, 252, 379, 442, 460, 957, 1119
FokI GGATG 5 cut(s) 289, 512, 643, 808, 812
FriOI GRGCYC 1 cut(s) 198
Fsp4HI GCNGC 8 cut(s) 240, 243, 252, 379, 442, 460, 957, 1119
FspBI CTAG 2 cut(s) 95, 296
GlaI GCGC 2 cut(s) 62, 618
GluI GCNGC 8 cut(s) 240, 243, 252, 379, 442, 460, 957, 1119
HaeIII GGCC 2 cut(s) 223, 884
HapII CCGG 1 cut(s) 604
HgaI GACGC 3 cut(s) 623, 662, 1055
HhaI GCGC 2 cut(s) 63, 619
Hin1I GRCGYC 1 cut(s) 351
Hin6I GCGC 2 cut(s) 61, 617
HinP1I GCGC 2 cut(s) 61, 617
HinfI GANTC 7 cut(s) 269, 308, 432, 535, 625, 728, 935
HpaII CCGG 1 cut(s) 604
HphI GGTGA 4 cut(s) 20, 476, 541, 1028
Hpy166II GTNNAC 1 cut(s) 100
Hpy188III TCNNGA 4 cut(s) 355, 622, 737, 1097
Hpy8I GTNNAC 1 cut(s) 100
Hpy99I CGWCG 4 cut(s) 283, 353, 356, 617
HpyAV CCTTC 3 cut(s) 502, 916, 1108
HpyCH4III ACNGT 2 cut(s) 322, 390
HpyCH4IV ACGT 1 cut(s) 351
HpyCH4V TGCA 9 cut(s) 87, 239, 779, 842, 904, 909, 959, 973, 1106
HpyF10VI GCNNNNNNNGC 4 cut(s) 236, 248, 251, 687
HpyF3I CTNAG 3 cut(s) 197, 455, 1053
HpySE526I ACGT 1 cut(s) 351
Hsp92I GRCGYC 1 cut(s) 351
HspAI GCGC 2 cut(s) 61, 617
KspI CCGCGG 1 cut(s) 255
Kzo9I GATC 3 cut(s) 298, 484, 1018
LmnI GCTCC 5 cut(s) 128, 280, 325, 540, 695
Lsp1109I GCAGC 5 cut(s) 251, 254, 471, 943, 1130
LweI GCATC 2 cut(s) 665, 790
MaeI CTAG 2 cut(s) 95, 296
MaeII ACGT 1 cut(s) 351
MaeIII GTNAC 2 cut(s) 211, 650
MalI GATC 3 cut(s) 300, 486, 1020
MboI GATC 3 cut(s) 298, 484, 1018
MboII GAAGA 3 cut(s) 105, 1037, 1043
MfeI CAATTG 1 cut(s) 234
MflI RGATCY 1 cut(s) 298
MhlI GDGCHC 3 cut(s) 198, 330, 645
MluCI AATT 7 cut(s) 67, 140, 234, 774, 1041, 1078, 1083
MlyI GAGTC 2 cut(s) 317, 722
MmeI TCCRAC 3 cut(s) 157, 690, 832
MslI CAYNNNNRTG 2 cut(s) 968, 1101
MspA1I CMGCKG 2 cut(s) 254, 462
MspI CCGG 1 cut(s) 604
MspR9I CCNGG 2 cut(s) 887, 1004
MunI CAATTG 1 cut(s) 234
Mva1269I GAATGC 1 cut(s) 963
MvaI CCWGG 2 cut(s) 887, 1004
MvnI CGCG 4 cut(s) 61, 254, 617, 763
MwoI GCNNNNNNNGC 4 cut(s) 236, 248, 251, 687
NdeII GATC 3 cut(s) 298, 484, 1018
NlaIV GGNNCC 3 cut(s) 124, 392, 416
NmeAIII GCCGAG 1 cut(s) 199
NmuCI GTSAC 2 cut(s) 211, 650
NspI RCATGY 1 cut(s) 791
PagI TCATGA 1 cut(s) 1096
PciI ACATGT 1 cut(s) 787
PctI GAATGC 1 cut(s) 963
PfeI GAWTC 5 cut(s) 269, 432, 535, 625, 935
Pfl23II CGTACG 1 cut(s) 225
PflFI GACNNNGTC 1 cut(s) 350
PkrI GCNGC 8 cut(s) 241, 244, 253, 380, 443, 461, 958, 1120
PleI GAGTC 2 cut(s) 316, 722
PpsI GAGTC 2 cut(s) 316, 722
PscI ACATGT 1 cut(s) 787
Psp6I CCWGG 2 cut(s) 885, 1002
PspGI CCWGG 2 cut(s) 885, 1002
PspLI CGTACG 1 cut(s) 225
PspN4I GGNNCC 3 cut(s) 124, 392, 416
PspPI GGNCC 4 cut(s) 390, 606, 828, 883
PstI CTGCAG 1 cut(s) 961
PstNI CAGNNNCTG 1 cut(s) 956
PsuI RGATCY 1 cut(s) 298
PsyI GACNNNGTC 1 cut(s) 350
RsaI GTAC 4 cut(s) 227, 422, 491, 668
RsaNI GTAC 4 cut(s) 226, 421, 490, 667
RseI CAYNNNNRTG 2 cut(s) 968, 1101
Rsr2I CGGWCCG 1 cut(s) 606
RsrII CGGWCCG 1 cut(s) 606
SacII CCGCGG 1 cut(s) 255
SatI GCNGC 8 cut(s) 240, 243, 252, 379, 442, 460, 957, 1119
Sau3AI GATC 3 cut(s) 298, 484, 1018
Sau96I GGNCC 4 cut(s) 390, 606, 828, 883
SchI GAGTC 2 cut(s) 317, 722
ScrFI CCNGG 2 cut(s) 887, 1004
SduI GDGCHC 3 cut(s) 198, 330, 645
SfaNI GCATC 2 cut(s) 665, 790
SfcI CTRYAG 1 cut(s) 957
Sfr303I CCGCGG 1 cut(s) 255
SgrBI CCGCGG 1 cut(s) 255
SinI GGWCC 3 cut(s) 390, 606, 828
SmiMI CAYNNNNRTG 2 cut(s) 968, 1101
SmlI CTYRAG 1 cut(s) 735
SmoI CTYRAG 1 cut(s) 735
Sse9I AATT 7 cut(s) 67, 140, 234, 774, 1041, 1078, 1083
SspMI CTAG 2 cut(s) 95, 296
StyD4I CCNGG 2 cut(s) 885, 1002
StyI CCWWGG 1 cut(s) 1133
TaaI ACNGT 2 cut(s) 322, 390
TaiI ACGT 1 cut(s) 354
TaqI TCGA 2 cut(s) 426, 772
TasI AATT 7 cut(s) 67, 140, 234, 774, 1041, 1078, 1083
TauI GCSGC 3 cut(s) 254, 381, 444
TfiI GAWTC 5 cut(s) 269, 432, 535, 625, 935
TseFI GTSAC 2 cut(s) 211, 650
TseI GCWGC 5 cut(s) 239, 242, 459, 956, 1118
Tsp45I GTSAC 2 cut(s) 211, 650
TspDTI ATGAA 5 cut(s) 17, 133, 380, 626, 935
TspGWI ACGGA 2 cut(s) 267, 477
Tth111I GACNNNGTC 1 cut(s) 350
VpaK11BI GGWCC 3 cut(s) 390, 606, 828
XapI RAATTY 3 cut(s) 67, 1041, 1078
XceI RCATGY 1 cut(s) 791
XspI CTAG 2 cut(s) 95, 296
ZraI GACGTC 1 cut(s) 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.