Rmu_sc0005310.1_g000038

Coiled-coil domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005310.1
OG0005457
Physical Location & Seq
Reverse (-)
203077 .. 205295
2219 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 603 bp
atgaagcatacaacagcagctggtcttccaaaaaaaacagagagagagaacagcagctgccctaatttggtgtctccagtctcgcctcctcctcctgctgctgctaacagtctgcgatttaggcaaatggctgctgaagaggactccctcgaagaaatcgttgcggcacgaaaggagaggctcagagccctcaaagctgcacagcaactgttaaacactccggatgaggactctgctcaacttgaaagtaacactaacgatgaccaggaaaagagcaatgaaactcctgatggggatgaaactaccaacatgaaattccgaaattatgttcctcatgataagaagcttcaggaagggaagctagatccaccaaaaccatccaagtttgaagaccctgttgcagcagtgcctcctccatcagagaagaaagaggacccattcgtgaatattgctcctaaaaaaccaaactgggaacttcgtagggatgtgcagaagaagcttgataagcttgaaaggcgaacccagaaggcaatgtgtaaacttatggaacaagaaaagcagaggcaactggatgaagacagcatcaatggtgcaggggactaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
cwf18

Protein Analysis

200

Amino Acids

22.52

Weight (kDa)

5.66

Isoelectric Point (pI)

59.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cwf18 PF08315 49 - 184 1.8e-31 cwf18 pre-mRNA splicing factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 220
AciI CCGC 1 cut(s) 164
AclWI GGATC 1 cut(s) 359
AcsI RAATTY 1 cut(s) 314
AcuI CTGAAG 2 cut(s) 156, 332
AfiI CCNNNNNNNGG 1 cut(s) 67
AgsI TTSAA 3 cut(s) 245, 389, 512
AjnI CCWGG 1 cut(s) 264
AjuI GAANNNNNNNTTGG 2 cut(s) 299, 331
AluBI AGCT 7 cut(s) 20, 57, 197, 346, 361, 499, 508
AluI AGCT 7 cut(s) 20, 57, 197, 346, 361, 499, 508
Alw26I GTCTC 2 cut(s) 78, 85
AlwI GGATC 1 cut(s) 359
AlwNI CAGNNNCTG 3 cut(s) 20, 57, 208
Aor13HI TCCGGA 1 cut(s) 220
ApeKI GCWGC 8 cut(s) 17, 54, 57, 98, 101, 131, 197, 401
ApoI RAATTY 1 cut(s) 314
AspS9I GGNCC 1 cut(s) 433
AvaII GGWCC 1 cut(s) 433
BanII GRGCYC 1 cut(s) 190
BbsI GAAGAC 3 cut(s) 17, 396, 582
BbvI GCAGC 8 cut(s) 29, 44, 66, 85, 88, 118, 184, 413
BccI CCATC 3 cut(s) 284, 385, 424
BciT130I CCWGG 1 cut(s) 266
BcoDI GTCTC 2 cut(s) 78, 85
BfaI CTAG 1 cut(s) 362
BisI GCNGC 9 cut(s) 18, 55, 58, 99, 102, 132, 165, 198, 402
BlsI GCNGC 9 cut(s) 19, 56, 59, 100, 103, 133, 166, 199, 403
Bme1390I CCNGG 1 cut(s) 266
Bme18I GGWCC 1 cut(s) 433
BmgT120I GGNCC 1 cut(s) 433
BmiI GGNNCC 1 cut(s) 435
BmrFI CCNGG 1 cut(s) 266
BmrI ACTGGG 1 cut(s) 478
BmsI GCATC 1 cut(s) 591
BmuI ACTGGG 1 cut(s) 478
BpiI GAAGAC 3 cut(s) 17, 396, 582
BpmI CTGGAG 1 cut(s) 60
BsaWI WCCGGW 1 cut(s) 220
Bsc4I CCNNNNNNNGG 1 cut(s) 67
Bse1I ACTGG 3 cut(s) 77, 473, 573
Bse3DI GCAATG 2 cut(s) 283, 537
BseAI TCCGGA 1 cut(s) 220
BseBI CCWGG 1 cut(s) 266
BseGI GGATG 5 cut(s) 229, 301, 377, 490, 577
BseLI CCNNNNNNNGG 1 cut(s) 67
BseMI GCAATG 2 cut(s) 283, 537
BseMII CTCAG 1 cut(s) 196
BseNI ACTGG 3 cut(s) 77, 473, 573
BseRI GAGGAG 3 cut(s) 78, 81, 402
BseXI GCAGC 8 cut(s) 29, 44, 66, 85, 88, 118, 184, 413
BsgI GTGCAG 2 cut(s) 183, 509
BsiSI CCGG 1 cut(s) 221
BslI CCNNNNNNNGG 1 cut(s) 67
BsmAI GTCTC 2 cut(s) 78, 85
Bsp1286I GDGCHC 1 cut(s) 190
Bsp13I TCCGGA 1 cut(s) 220
Bsp143I GATC 1 cut(s) 364
BspACI CCGC 1 cut(s) 164
BspCNI CTCAG 1 cut(s) 195
BspEI TCCGGA 1 cut(s) 220
BspHI TCATGA 1 cut(s) 334
BspLI GGNNCC 1 cut(s) 435
BspPI GGATC 1 cut(s) 359
BsrDI GCAATG 2 cut(s) 283, 537
BsrI ACTGG 3 cut(s) 77, 473, 573
BssMI GATC 1 cut(s) 364
Bst2UI CCWGG 1 cut(s) 266
Bst4CI ACNGT 2 cut(s) 110, 210
Bst6I CTCTTC 1 cut(s) 132
BstDEI CTNAG 1 cut(s) 182
BstF5I GGATG 5 cut(s) 229, 301, 377, 490, 577
BstKTI GATC 1 cut(s) 367
BstMAI GTCTC 2 cut(s) 78, 85
BstMBI GATC 1 cut(s) 364
BstMWI GCNNNNNNNGC 5 cut(s) 121, 194, 496, 505, 514
BstNI CCWGG 1 cut(s) 266
BstSCI CCNGG 1 cut(s) 264
BstV1I GCAGC 8 cut(s) 29, 44, 66, 85, 88, 118, 184, 413
BstV2I GAAGAC 3 cut(s) 17, 396, 582
BstX2I RGATCY 1 cut(s) 364
BstYI RGATCY 1 cut(s) 364
BtsCI GGATG 5 cut(s) 229, 301, 377, 490, 577
BtsI GCAGTG 1 cut(s) 411
BtsIMutI CAGTG 1 cut(s) 411
CaiI CAGNNNCTG 3 cut(s) 20, 57, 208
CciI TCATGA 1 cut(s) 334
Cfr13I GGNCC 1 cut(s) 433
CviAII CATG 2 cut(s) 310, 335
DdeI CTNAG 1 cut(s) 182
DpnI GATC 1 cut(s) 366
DpnII GATC 1 cut(s) 364
Eam1104I CTCTTC 1 cut(s) 132
EarI CTCTTC 1 cut(s) 132
Eco24I GRGCYC 1 cut(s) 190
Eco47I GGWCC 1 cut(s) 433
Eco57I CTGAAG 2 cut(s) 156, 332
EcoO109I RGGNCCY 1 cut(s) 433
EcoRII CCWGG 1 cut(s) 264
EcoT38I GRGCYC 1 cut(s) 190
FaeI CATG 2 cut(s) 313, 338
FaiI YATR 5 cut(s) 9, 311, 327, 336, 545
FatI CATG 2 cut(s) 309, 334
Fnu4HI GCNGC 9 cut(s) 18, 55, 58, 99, 102, 132, 165, 198, 402
FokI GGATG 5 cut(s) 236, 308, 364, 497, 584
FriOI GRGCYC 1 cut(s) 190
Fsp4HI GCNGC 9 cut(s) 18, 55, 58, 99, 102, 132, 165, 198, 402
FspBI CTAG 1 cut(s) 362
GluI GCNGC 9 cut(s) 18, 55, 58, 99, 102, 132, 165, 198, 402
GsuI CTGGAG 1 cut(s) 60
HapII CCGG 1 cut(s) 221
Hin1II CATG 2 cut(s) 313, 338
HindIII AAGCTT 3 cut(s) 344, 497, 506
HinfI GANTC 2 cut(s) 143, 230
HpaII CCGG 1 cut(s) 221
Hpy166II GTNNAC 1 cut(s) 539
Hpy188I TCNGA 3 cut(s) 185, 320, 421
Hpy188III TCNNGA 5 cut(s) 221, 287, 335, 350, 442
Hpy8I GTNNAC 1 cut(s) 539
HpyAV CCTTC 2 cut(s) 347, 520
HpyCH4III ACNGT 2 cut(s) 110, 210
HpyCH4V TGCA 4 cut(s) 200, 401, 490, 593
HpyF10VI GCNNNNNNNGC 5 cut(s) 121, 194, 496, 505, 514
HpyF3I CTNAG 1 cut(s) 182
Hsp92II CATG 2 cut(s) 313, 338
Kpn2I TCCGGA 1 cut(s) 220
Kzo9I GATC 1 cut(s) 364
LmnI GCTCC 1 cut(s) 457
Lsp1109I GCAGC 8 cut(s) 29, 44, 66, 85, 88, 118, 184, 413
LweI GCATC 1 cut(s) 591
MaeI CTAG 1 cut(s) 362
MaeIII GTNAC 1 cut(s) 248
MalI GATC 1 cut(s) 366
MboI GATC 1 cut(s) 364
MboII GAAGA 7 cut(s) 17, 149, 164, 401, 436, 505, 587
MflI RGATCY 1 cut(s) 364
MhlI GDGCHC 1 cut(s) 190
MluCI AATT 3 cut(s) 64, 314, 322
MlyI GAGTC 2 cut(s) 137, 224
MroI TCCGGA 1 cut(s) 220
MseI TTAA 1 cut(s) 212
MspA1I CMGCKG 2 cut(s) 20, 57
MspI CCGG 1 cut(s) 221
MspR9I CCNGG 1 cut(s) 266
MvaI CCWGG 1 cut(s) 266
MwoI GCNNNNNNNGC 5 cut(s) 121, 194, 496, 505, 514
NdeII GATC 1 cut(s) 364
NlaIII CATG 2 cut(s) 313, 338
NlaIV GGNNCC 1 cut(s) 435
PagI TCATGA 1 cut(s) 334
PcsI WCGNNNNNNNCGW 1 cut(s) 156
PkrI GCNGC 9 cut(s) 19, 56, 59, 100, 103, 133, 166, 199, 403
PleI GAGTC 2 cut(s) 137, 224
PpsI GAGTC 2 cut(s) 137, 224
PpuMI RGGWCCY 1 cut(s) 433
Psp5II RGGWCCY 1 cut(s) 433
Psp6I CCWGG 1 cut(s) 264
PspGI CCWGG 1 cut(s) 264
PspN4I GGNNCC 1 cut(s) 435
PspPI GGNCC 1 cut(s) 433
PspPPI RGGWCCY 1 cut(s) 433
PstNI CAGNNNCTG 3 cut(s) 20, 57, 208
PsuI RGATCY 1 cut(s) 364
PvuII CAGCTG 2 cut(s) 20, 57
SaqAI TTAA 1 cut(s) 212
SatI GCNGC 9 cut(s) 18, 55, 58, 99, 102, 132, 165, 198, 402
Sau3AI GATC 1 cut(s) 364
Sau96I GGNCC 1 cut(s) 433
SchI GAGTC 2 cut(s) 137, 224
ScrFI CCNGG 1 cut(s) 266
SduI GDGCHC 1 cut(s) 190
SetI ASST 7 cut(s) 22, 59, 199, 348, 363, 501, 510
SfaNI GCATC 1 cut(s) 591
SinI GGWCC 1 cut(s) 433
Sse9I AATT 3 cut(s) 64, 314, 322
SsiI CCGC 1 cut(s) 164
SspI AATATT 1 cut(s) 448
SspMI CTAG 1 cut(s) 362
StyD4I CCNGG 1 cut(s) 264
TaaI ACNGT 2 cut(s) 110, 210
TaqI TCGA 1 cut(s) 150
TasI AATT 3 cut(s) 64, 314, 322
TauI GCSGC 1 cut(s) 167
Tru1I TTAA 1 cut(s) 212
Tru9I TTAA 1 cut(s) 212
TscAI CASTG 1 cut(s) 411
TseI GCWGC 8 cut(s) 17, 54, 57, 98, 101, 131, 197, 401
TspDTI ATGAA 5 cut(s) 17, 294, 312, 326, 588
TspRI CASTG 1 cut(s) 411
VpaK11BI GGWCC 1 cut(s) 433
XapI RAATTY 1 cut(s) 314
XspI CTAG 1 cut(s) 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.