Rmu_sc0005514.1_g000015

Phosphatidylinositol-glycan biosynthesis class X

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005514.1
OG0007198
Physical Location & Seq
Reverse (-)
63466 .. 65732
2267 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 777 bp
atgacgcctcatcaagatgagagcggtggcattatttctagccgtacgctcaaatatgagctcgggatttggtgcttgaattacggatactgcttcaatcctgggttaggattgcagatgtcgttcgatcctaagctaggattgcagtggctgttcaatcctaagctgggattgcaagttatttataatcctggtcgtggcttgatggttcgaagtagcaaaacagagctgcaggtgctcaggcgctcaagcagggagcagggagagcaggggacgcaacgcggggcgtgctcaatgcaggctgctgtcttggcagtgtacagcaatatagctgtctttggagatacaaatttggaattgccttcttttctttccaatcgttctgctgtcgaggttcacatggatgttgatcatggtctcttgttgggtccgagtaaagagtctaccatcaatttagagcttcccctacatgcacgatatccacctctagatgaaactggctattgggaagtcaaattcggtacacctgatttcttcatgcgttgtggtgttgagggaaagtcacataatcaaagctgtttatataggataccagatattgatggttctgattcaacatttggtatggttgtctgggacataccttctgcgatgaaggcacatattcgaattgtttccatctacacttttattgctgccttaatttcagccatcttgattgctgtaacatgcatattttattcagataactccggcaaacattcaaaggaatcttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
PIG-X

Protein Analysis

258

Amino Acids

28.62

Weight (kDa)

6.16

Isoelectric Point (pI)

39.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIG-X PF08320 108 - 243 1.1e-11 PIG-X / PBN1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 186
AarI CACCTGC 1 cut(s) 223
Acc36I ACCTGC 1 cut(s) 223
AccBSI CCGCTC 1 cut(s) 24
AccI GTMKAC 1 cut(s) 443
AccII CGCG 1 cut(s) 282
AciI CCGC 2 cut(s) 24, 282
AclWI GGATC 1 cut(s) 122
AcsI RAATTY 2 cut(s) 349, 515
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 3 cut(s) 46, 320, 523
AfiI CCNNNNNNNGG 4 cut(s) 107, 137, 167, 197
AgsI TTSAA 5 cut(s) 79, 97, 157, 615, 765
AjnI CCWGG 2 cut(s) 100, 190
AluBI AGCT 7 cut(s) 61, 136, 166, 229, 332, 460, 576
AluI AGCT 7 cut(s) 61, 136, 166, 229, 332, 460, 576
Alw21I GWGCWC 3 cut(s) 63, 240, 293
Alw26I GTCTC 1 cut(s) 422
AlwI GGATC 1 cut(s) 122
AlwNI CAGNNNCTG 1 cut(s) 151
Ama87I CYCGRG 1 cut(s) 62
ApeKI GCWGC 3 cut(s) 229, 302, 695
ApoI RAATTY 2 cut(s) 349, 515
Asp700I GAANNNNTTC 1 cut(s) 673
AspLEI GCGC 1 cut(s) 246
AspS9I GGNCC 1 cut(s) 428
AsuII TTCGAA 2 cut(s) 211, 667
AvaI CYCGRG 1 cut(s) 62
AvaII GGWCC 1 cut(s) 428
BanII GRGCYC 1 cut(s) 63
Bbv12I GWGCWC 3 cut(s) 63, 240, 293
BbvI GCAGC 3 cut(s) 216, 289, 682
BccI CCATC 5 cut(s) 199, 455, 596, 686, 719
BceAI ACGGC 1 cut(s) 27
BciT130I CCWGG 2 cut(s) 102, 192
BciVI GTATCC 2 cut(s) 80, 582
BclI TGATCA 1 cut(s) 409
BcoDI GTCTC 1 cut(s) 422
BfaI CTAG 3 cut(s) 39, 137, 488
BfmI CTRYAG 1 cut(s) 230
BfoI RGCGCY 1 cut(s) 247
BfuAI ACCTGC 1 cut(s) 223
BfuI GTATCC 2 cut(s) 80, 582
BisI GCNGC 3 cut(s) 230, 303, 696
BlsI GCNGC 3 cut(s) 231, 304, 697
Bme1390I CCNGG 2 cut(s) 102, 192
Bme18I GGWCC 1 cut(s) 428
BmeT110I CYCGRG 1 cut(s) 62
BmgT120I GGNCC 1 cut(s) 428
BmiI GGNNCC 1 cut(s) 429
BmrFI CCNGG 2 cut(s) 102, 192
Bpu10I CCTNAGC 3 cut(s) 132, 162, 239
Bpu14I TTCGAA 2 cut(s) 211, 667
BpuEI CTTGAG 1 cut(s) 232
BsaBI GATNNNNATC 1 cut(s) 408
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 422
BsaJI CCNNGG 1 cut(s) 101
Bsc4I CCNNNNNNNGG 4 cut(s) 107, 137, 167, 197
Bse1I ACTGG 1 cut(s) 502
Bse8I GATNNNNATC 1 cut(s) 408
BseBI CCWGG 2 cut(s) 102, 192
BseDI CCNNGG 1 cut(s) 101
BseGI GGATG 1 cut(s) 409
BseJI GATNNNNATC 1 cut(s) 408
BseLI CCNNNNNNNGG 4 cut(s) 107, 137, 167, 197
BseMII CTCAG 1 cut(s) 253
BseNI ACTGG 1 cut(s) 502
BseXI GCAGC 3 cut(s) 216, 289, 682
BseYI CCCAGC 1 cut(s) 166
Bsh1236I CGCG 1 cut(s) 282
BsiHKAI GWGCWC 3 cut(s) 63, 240, 293
BsiHKCI CYCGRG 1 cut(s) 62
BsiSI CCGG 1 cut(s) 753
BsiWI CGTACG 1 cut(s) 44
BslFI GGGAC 2 cut(s) 286, 650
BslI CCNNNNNNNGG 4 cut(s) 107, 137, 167, 197
BsmAI GTCTC 1 cut(s) 422
BsmFI GGGAC 2 cut(s) 286, 650
Bso31I GGTCTC 1 cut(s) 422
BsoBI CYCGRG 1 cut(s) 62
Bsp119I TTCGAA 2 cut(s) 211, 667
Bsp1286I GDGCHC 3 cut(s) 63, 240, 293
Bsp1407I TGTACA 1 cut(s) 318
Bsp143I GATC 2 cut(s) 127, 409
BspACI CCGC 2 cut(s) 24, 282
BspCNI CTCAG 1 cut(s) 252
BspFNI CGCG 1 cut(s) 282
BspLI GGNNCC 1 cut(s) 429
BspMAI CTGCAG 1 cut(s) 234
BspMI ACCTGC 1 cut(s) 223
BspPI GGATC 1 cut(s) 122
BspT104I TTCGAA 2 cut(s) 211, 667
BspTNI GGTCTC 1 cut(s) 422
BsrBI CCGCTC 1 cut(s) 24
BsrGI TGTACA 1 cut(s) 318
BsrI ACTGG 1 cut(s) 502
BssECI CCNNGG 1 cut(s) 101
BssMI GATC 2 cut(s) 127, 409
BssNI GRCGYC 1 cut(s) 5
Bst2UI CCWGG 2 cut(s) 102, 192
BstACI GRCGYC 1 cut(s) 5
BstAUI TGTACA 1 cut(s) 318
BstBI TTCGAA 2 cut(s) 211, 667
BstC8I GCNNGC 2 cut(s) 289, 300
BstDEI CTNAG 4 cut(s) 132, 162, 239, 774
BstENI CCTNNNNNAGG 2 cut(s) 105, 135
BstF5I GGATG 1 cut(s) 409
BstFNI CGCG 1 cut(s) 282
BstH2I RGCGCY 1 cut(s) 247
BstHHI GCGC 1 cut(s) 246
BstKTI GATC 2 cut(s) 130, 412
BstMAI GTCTC 1 cut(s) 422
BstMBI GATC 2 cut(s) 127, 409
BstMWI GCNNNNNNNGC 8 cut(s) 142, 172, 235, 265, 274, 288, 311, 656
BstNI CCWGG 2 cut(s) 102, 192
BstNSI RCATGY 2 cut(s) 473, 732
BstSCI CCNGG 2 cut(s) 100, 190
BstSFI CTRYAG 1 cut(s) 230
BstUI CGCG 1 cut(s) 282
BstV1I GCAGC 3 cut(s) 216, 289, 682
BsuI GTATCC 2 cut(s) 80, 582
BtgZI GCGATG 1 cut(s) 665
BtsCI GGATG 1 cut(s) 409
BtsI GCAGTG 2 cut(s) 152, 321
BtsIMutI CAGTG 2 cut(s) 152, 321
BveI ACCTGC 1 cut(s) 223
Cac8I GCNNGC 2 cut(s) 289, 300
CaiI CAGNNNCTG 1 cut(s) 151
CfoI GCGC 1 cut(s) 246
Cfr13I GGNCC 1 cut(s) 428
CseI GACGC 2 cut(s) 13, 283
Csp6I GTAC 3 cut(s) 45, 319, 522
CviAII CATG 5 cut(s) 400, 413, 470, 538, 729
CviQI GTAC 3 cut(s) 45, 319, 522
DdeI CTNAG 4 cut(s) 132, 162, 239, 774
DpnI GATC 2 cut(s) 129, 411
DpnII GATC 2 cut(s) 127, 409
Ecl136II GAGCTC 1 cut(s) 61
Eco24I GRGCYC 1 cut(s) 63
Eco31I GGTCTC 1 cut(s) 422
Eco32I GATATC 1 cut(s) 479
Eco47I GGWCC 1 cut(s) 428
Eco53kI GAGCTC 1 cut(s) 61
Eco88I CYCGRG 1 cut(s) 62
EcoICRI GAGCTC 1 cut(s) 61
EcoNI CCTNNNNNAGG 2 cut(s) 105, 135
EcoRII CCWGG 2 cut(s) 100, 190
EcoRV GATATC 1 cut(s) 479
EcoT22I ATGCAT 1 cut(s) 734
EcoT38I GRGCYC 1 cut(s) 63
FaeI CATG 5 cut(s) 403, 416, 473, 541, 732
FaqI GGGAC 2 cut(s) 286, 650
FatI CATG 5 cut(s) 399, 412, 469, 537, 728
FauI CCCGC 1 cut(s) 275
FbaI TGATCA 1 cut(s) 409
FblI GTMKAC 1 cut(s) 443
Fnu4HI GCNGC 3 cut(s) 230, 303, 696
FokI GGATG 1 cut(s) 416
FriOI GRGCYC 1 cut(s) 63
Fsp4HI GCNGC 3 cut(s) 230, 303, 696
FspBI CTAG 3 cut(s) 39, 137, 488
GlaI GCGC 1 cut(s) 245
GluI GCNGC 3 cut(s) 230, 303, 696
GsaI CCCAGC 1 cut(s) 170
HaeII RGCGCY 1 cut(s) 247
HapII CCGG 1 cut(s) 753
HgaI GACGC 2 cut(s) 13, 283
HhaI GCGC 1 cut(s) 246
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 5 cut(s) 403, 416, 473, 541, 732
Hin6I GCGC 1 cut(s) 244
HinP1I GCGC 1 cut(s) 244
HinfI GANTC 3 cut(s) 440, 611, 770
HpaII CCGG 1 cut(s) 753
Hpy166II GTNNAC 4 cut(s) 319, 397, 444, 524
Hpy188I TCNGA 3 cut(s) 432, 610, 745
Hpy188III TCNNGA 4 cut(s) 14, 64, 488, 715
Hpy8I GTNNAC 4 cut(s) 319, 397, 444, 524
HpyAV CCTTC 3 cut(s) 372, 649, 654
HpyCH4V TGCA 7 cut(s) 115, 145, 175, 232, 298, 473, 732
HpyF10VI GCNNNNNNNGC 8 cut(s) 142, 172, 235, 265, 274, 288, 311, 656
HpyF3I CTNAG 4 cut(s) 132, 162, 239, 774
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 5 cut(s) 403, 416, 473, 541, 732
HspAI GCGC 1 cut(s) 244
Ksp22I TGATCA 1 cut(s) 409
Kzo9I GATC 2 cut(s) 127, 409
LmnI GCTCC 1 cut(s) 256
Lsp1109I GCAGC 3 cut(s) 216, 289, 682
MaeI CTAG 3 cut(s) 39, 137, 488
MaeIII GTNAC 2 cut(s) 561, 724
MalI GATC 2 cut(s) 129, 411
MbiI CCGCTC 1 cut(s) 24
MboI GATC 2 cut(s) 127, 409
MboII GAAGA 1 cut(s) 526
MhlI GDGCHC 3 cut(s) 63, 240, 293
MluCI AATT 7 cut(s) 79, 349, 356, 451, 515, 669, 702
MlyI GAGTC 1 cut(s) 449
MnlI CCTC 4 cut(s) 18, 385, 495, 547
Mph1103I ATGCAT 1 cut(s) 734
MroXI GAANNNNTTC 1 cut(s) 673
MseI TTAA 1 cut(s) 701
MslI CAYNNNNRTG 2 cut(s) 15, 402
MspI CCGG 1 cut(s) 753
MspR9I CCNGG 2 cut(s) 102, 192
MvaI CCWGG 2 cut(s) 102, 192
MvnI CGCG 1 cut(s) 282
MwoI GCNNNNNNNGC 8 cut(s) 142, 172, 235, 265, 274, 288, 311, 656
NdeII GATC 2 cut(s) 127, 409
NlaIII CATG 5 cut(s) 403, 416, 473, 541, 732
NlaIV GGNNCC 1 cut(s) 429
NmuCI GTSAC 1 cut(s) 561
NsiI ATGCAT 1 cut(s) 734
NspI RCATGY 2 cut(s) 473, 732
NspV TTCGAA 2 cut(s) 211, 667
PaqCI CACCTGC 1 cut(s) 223
PdmI GAANNNNTTC 1 cut(s) 673
PfeI GAWTC 2 cut(s) 611, 770
Pfl23II CGTACG 1 cut(s) 44
PkrI GCNGC 3 cut(s) 231, 304, 697
PleI GAGTC 1 cut(s) 448
PpsI GAGTC 1 cut(s) 448
PsiI TTATAA 1 cut(s) 186
Psp124BI GAGCTC 1 cut(s) 63
Psp6I CCWGG 2 cut(s) 100, 190
PspFI CCCAGC 1 cut(s) 166
PspGI CCWGG 2 cut(s) 100, 190
PspLI CGTACG 1 cut(s) 44
PspN4I GGNNCC 1 cut(s) 429
PspPI GGNCC 1 cut(s) 428
PstI CTGCAG 1 cut(s) 234
PstNI CAGNNNCTG 1 cut(s) 151
RsaI GTAC 3 cut(s) 46, 320, 523
RsaNI GTAC 3 cut(s) 45, 319, 522
RseI CAYNNNNRTG 2 cut(s) 15, 402
SacI GAGCTC 1 cut(s) 63
SaqAI TTAA 1 cut(s) 701
SatI GCNGC 3 cut(s) 230, 303, 696
Sau3AI GATC 2 cut(s) 127, 409
Sau96I GGNCC 1 cut(s) 428
SchI GAGTC 1 cut(s) 449
ScrFI CCNGG 2 cut(s) 102, 192
SduI GDGCHC 3 cut(s) 63, 240, 293
SfcI CTRYAG 1 cut(s) 230
SfuI TTCGAA 2 cut(s) 211, 667
SinI GGWCC 1 cut(s) 428
SmiMI CAYNNNNRTG 2 cut(s) 15, 402
SmlI CTYRAG 1 cut(s) 247
SmoI CTYRAG 1 cut(s) 247
Sse9I AATT 7 cut(s) 79, 349, 356, 451, 515, 669, 702
SsiI CCGC 2 cut(s) 24, 282
SspMI CTAG 3 cut(s) 39, 137, 488
SstI GAGCTC 1 cut(s) 63
StyD4I CCNGG 2 cut(s) 100, 190
TaqI TCGA 4 cut(s) 126, 211, 390, 667
TasI AATT 7 cut(s) 79, 349, 356, 451, 515, 669, 702
TatI WGTACW 1 cut(s) 318
TfiI GAWTC 2 cut(s) 611, 770
Tru1I TTAA 1 cut(s) 701
Tru9I TTAA 1 cut(s) 701
TscAI CASTG 2 cut(s) 152, 321
TseFI GTSAC 1 cut(s) 561
TseI GCWGC 3 cut(s) 229, 302, 695
Tsp45I GTSAC 1 cut(s) 561
TspDTI ATGAA 3 cut(s) 507, 526, 668
TspGWI ACGGA 1 cut(s) 99
TspRI CASTG 2 cut(s) 152, 321
VpaK11BI GGWCC 1 cut(s) 428
XagI CCTNNNNNAGG 2 cut(s) 105, 135
XapI RAATTY 2 cut(s) 349, 515
XbaI TCTAGA 1 cut(s) 487
XceI RCATGY 2 cut(s) 473, 732
XmiI GTMKAC 1 cut(s) 443
XmnI GAANNNNTTC 1 cut(s) 673
XspI CTAG 3 cut(s) 39, 137, 488
Zsp2I ATGCAT 1 cut(s) 734
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.