Rmu_sc0005679.1_g000008

Coiled-coil domain-containing protein 130-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005679.1
OG0006850
Physical Location & Seq
Reverse (-)
25312 .. 25896
585 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 585 bp
atgaaggctgcatgctgcaaagaggaaattgttattctgacagatccgaagaattgtcagtatgtgattattagtggtgctcaacaaaagaccgaggattttgacaatgaggatgcggaaacccttgcgcctcctgcagatgaagaaagaggtatgttggcggatccattttatcgtcttgaacatcaagaaaaggattggaaaaagaagaagcaagccgagccaatgctactcctcttaaagagaatttccgaccaaaggcattcagatgactattcccttagcaagtccctgagggccaaacttagatctcaaaagaaaagagtcgctgaagaagaggctgtttcaaggagaatgggcctcaatatacgactgcttccagtagcagaagaggacactgccactgcgtcacaggtgaagttctctaccaagtttgacaaaagtagaagggataagcgagctttgattaatgcttcttcaatctttcctgggtcatctacgtttcccatcgtcaataagaaaattctgggactggaatttaagagaaggaaagtaagtgctgctgcagcatcctgcgagatttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF572

Protein Analysis

194

Amino Acids

22.02

Weight (kDa)

9.21

Isoelectric Point (pI)

65.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Saf4_Yju2 PF04502 1 - 152 2.5e-22 Saf4/Yju2 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 116, 161
AclWI GGATC 3 cut(s) 38, 158, 171
AcsI RAATTY 3 cut(s) 246, 522, 536
AcuI CTGAAG 1 cut(s) 351
AfiI CCNNNNNNNGG 1 cut(s) 258
AgsI TTSAA 3 cut(s) 182, 348, 480
AjnI CCWGG 1 cut(s) 487
AluBI AGCT 1 cut(s) 461
AluI AGCT 1 cut(s) 461
Alw21I GWGCWC 1 cut(s) 82
AlwI GGATC 3 cut(s) 38, 158, 171
AoxI GGCC 2 cut(s) 297, 358
ApeKI GCWGC 5 cut(s) 8, 15, 560, 563, 566
ApoI RAATTY 3 cut(s) 246, 522, 536
AseI ATTAAT 1 cut(s) 468
AspLEI GCGC 1 cut(s) 130
AspS9I GGNCC 2 cut(s) 297, 358
AsuHPI GGTGA 1 cut(s) 427
AxyI CCTNAGG 1 cut(s) 293
BamHI GGATCC 1 cut(s) 163
Bbv12I GWGCWC 1 cut(s) 82
BbvI GCAGC 4 cut(s) 2, 547, 550, 578
BccI CCATC 1 cut(s) 515
BciT130I CCWGG 1 cut(s) 489
BfmI CTRYAG 2 cut(s) 135, 564
BglII AGATCT 1 cut(s) 308
BisI GCNGC 5 cut(s) 9, 16, 561, 564, 567
BlsI GCNGC 5 cut(s) 10, 17, 562, 565, 568
Bme1390I CCNGG 1 cut(s) 489
BmgT120I GGNCC 2 cut(s) 297, 358
BmiI GGNNCC 1 cut(s) 165
BmrFI CCNGG 1 cut(s) 489
BmsI GCATC 2 cut(s) 103, 578
Bpu10I CCTNAGC 1 cut(s) 281
BsaJI CCNNGG 2 cut(s) 93, 488
Bsc4I CCNNNNNNNGG 1 cut(s) 258
Bse1I ACTGG 2 cut(s) 380, 537
Bse21I CCTNAGG 1 cut(s) 293
BseBI CCWGG 1 cut(s) 489
BseDI CCNNGG 2 cut(s) 93, 488
BseGI GGATG 2 cut(s) 118, 569
BseLI CCNNNNNNNGG 1 cut(s) 258
BseMII CTCAG 1 cut(s) 284
BseNI ACTGG 2 cut(s) 380, 537
BseRI GAGGAG 1 cut(s) 224
BseXI GCAGC 4 cut(s) 2, 547, 550, 578
BshFI GGCC 2 cut(s) 299, 360
BsiHKAI GWGCWC 1 cut(s) 82
BslFI GGGAC 2 cut(s) 274, 543
BslI CCNNNNNNNGG 1 cut(s) 258
BsmFI GGGAC 2 cut(s) 274, 543
BsmI GAATGC 1 cut(s) 262
BsnI GGCC 2 cut(s) 299, 360
Bsp1286I GDGCHC 1 cut(s) 82
Bsp143I GATC 3 cut(s) 43, 163, 308
BspACI CCGC 2 cut(s) 116, 161
BspANI GGCC 2 cut(s) 299, 360
BspCNI CTCAG 1 cut(s) 285
BspLI GGNNCC 1 cut(s) 165
BspMAI CTGCAG 2 cut(s) 139, 568
BspPI GGATC 3 cut(s) 38, 158, 171
BsrI ACTGG 2 cut(s) 380, 537
BssECI CCNNGG 2 cut(s) 93, 488
BssMI GATC 3 cut(s) 43, 163, 308
Bst2UI CCWGG 1 cut(s) 489
Bst6I CTCTTC 2 cut(s) 330, 384
BstC8I GCNNGC 3 cut(s) 13, 216, 459
BstDEI CTNAG 3 cut(s) 281, 293, 305
BstF5I GGATG 2 cut(s) 118, 569
BstHHI GCGC 1 cut(s) 130
BstKTI GATC 3 cut(s) 46, 166, 311
BstMBI GATC 3 cut(s) 43, 163, 308
BstMWI GCNNNNNNNGC 3 cut(s) 134, 220, 566
BstNI CCWGG 1 cut(s) 489
BstNSI RCATGY 1 cut(s) 15
BstSCI CCNGG 1 cut(s) 487
BstSFI CTRYAG 2 cut(s) 135, 564
BstV1I GCAGC 4 cut(s) 2, 547, 550, 578
BstX2I RGATCY 3 cut(s) 43, 163, 308
BstYI RGATCY 3 cut(s) 43, 163, 308
Bsu36I CCTNAGG 1 cut(s) 293
BsuRI GGCC 2 cut(s) 299, 360
BtsCI GGATG 2 cut(s) 118, 569
BtsI GCAGTG 2 cut(s) 396, 402
BtsIMutI CAGTG 2 cut(s) 396, 402
Cac8I GCNNGC 3 cut(s) 13, 216, 459
CfoI GCGC 1 cut(s) 130
Cfr13I GGNCC 2 cut(s) 297, 358
CseI GACGC 1 cut(s) 396
CviAII CATG 1 cut(s) 12
CviJI RGCY 7 cut(s) 8, 218, 223, 299, 341, 360, 461
CviKI_1 RGCY 7 cut(s) 8, 218, 223, 299, 341, 360, 461
DdeI CTNAG 3 cut(s) 281, 293, 305
DpnI GATC 3 cut(s) 45, 165, 310
DpnII GATC 3 cut(s) 43, 163, 308
Eam1104I CTCTTC 2 cut(s) 330, 384
EarI CTCTTC 2 cut(s) 330, 384
EciI GGCGGA 1 cut(s) 176
Eco57I CTGAAG 1 cut(s) 351
Eco81I CCTNAGG 1 cut(s) 293
EcoRII CCWGG 1 cut(s) 487
FaeI CATG 1 cut(s) 15
FaiI YATR 4 cut(s) 13, 63, 155, 368
FaqI GGGAC 2 cut(s) 274, 543
FatI CATG 1 cut(s) 11
Fnu4HI GCNGC 5 cut(s) 9, 16, 561, 564, 567
FokI GGATG 2 cut(s) 125, 556
Fsp4HI GCNGC 5 cut(s) 9, 16, 561, 564, 567
GlaI GCGC 1 cut(s) 129
GluI GCNGC 5 cut(s) 9, 16, 561, 564, 567
HaeIII GGCC 2 cut(s) 299, 360
HgaI GACGC 1 cut(s) 396
HhaI GCGC 1 cut(s) 130
Hin1II CATG 1 cut(s) 15
Hin6I GCGC 1 cut(s) 128
HinP1I GCGC 1 cut(s) 128
HinfI GANTC 1 cut(s) 324
HphI GGTGA 1 cut(s) 427
Hpy188I TCNGA 4 cut(s) 39, 48, 253, 268
Hpy188III TCNNGA 2 cut(s) 179, 188
HpyAV CCTTC 2 cut(s) 441, 540
HpyCH4IV ACGT 1 cut(s) 500
HpyCH4V TGCA 4 cut(s) 11, 18, 137, 566
HpyF10VI GCNNNNNNNGC 3 cut(s) 134, 220, 566
HpyF3I CTNAG 3 cut(s) 281, 293, 305
HpySE526I ACGT 1 cut(s) 500
Hsp92II CATG 1 cut(s) 15
HspAI GCGC 1 cut(s) 128
Kzo9I GATC 3 cut(s) 43, 163, 308
LpnPI CCDG 8 cut(s) 147, 305, 393, 398, 474, 501, 512, 518
Lsp1109I GCAGC 4 cut(s) 2, 547, 550, 578
LweI GCATC 2 cut(s) 103, 578
MaeII ACGT 1 cut(s) 500
MaeIII GTNAC 1 cut(s) 408
MalI GATC 3 cut(s) 45, 165, 310
MboI GATC 3 cut(s) 43, 163, 308
MboII GAAGA 7 cut(s) 61, 155, 220, 344, 347, 401, 468
MflI RGATCY 3 cut(s) 43, 163, 308
MhlI GDGCHC 1 cut(s) 82
MluCI AATT 5 cut(s) 27, 52, 246, 522, 536
MlyI GAGTC 1 cut(s) 333
MmeI TCCRAC 1 cut(s) 276
MseI TTAA 3 cut(s) 239, 468, 540
MslI CAYNNNNRTG 1 cut(s) 267
MspR9I CCNGG 1 cut(s) 489
Mva1269I GAATGC 1 cut(s) 262
MvaI CCWGG 1 cut(s) 489
MwoI GCNNNNNNNGC 3 cut(s) 134, 220, 566
NdeII GATC 3 cut(s) 43, 163, 308
NlaIII CATG 1 cut(s) 15
NlaIV GGNNCC 1 cut(s) 165
NmeAIII GCCGAG 1 cut(s) 244
NmuCI GTSAC 1 cut(s) 408
NspI RCATGY 1 cut(s) 15
PaeI GCATGC 1 cut(s) 15
PctI GAATGC 1 cut(s) 262
PkrI GCNGC 5 cut(s) 10, 17, 562, 565, 568
PleI GAGTC 1 cut(s) 332
PpsI GAGTC 1 cut(s) 332
PshBI ATTAAT 1 cut(s) 468
Psp6I CCWGG 1 cut(s) 487
PspGI CCWGG 1 cut(s) 487
PspN4I GGNNCC 1 cut(s) 165
PspPI GGNCC 2 cut(s) 297, 358
PstI CTGCAG 2 cut(s) 139, 568
PsuI RGATCY 3 cut(s) 43, 163, 308
RseI CAYNNNNRTG 1 cut(s) 267
SaqAI TTAA 3 cut(s) 239, 468, 540
SatI GCNGC 5 cut(s) 9, 16, 561, 564, 567
Sau3AI GATC 3 cut(s) 43, 163, 308
Sau96I GGNCC 2 cut(s) 297, 358
SchI GAGTC 1 cut(s) 333
ScrFI CCNGG 1 cut(s) 489
SduI GDGCHC 1 cut(s) 82
SetI ASST 4 cut(s) 154, 417, 463, 503
SfaNI GCATC 2 cut(s) 103, 578
SfcI CTRYAG 2 cut(s) 135, 564
SmiMI CAYNNNNRTG 1 cut(s) 267
SphI GCATGC 1 cut(s) 15
Sse9I AATT 5 cut(s) 27, 52, 246, 522, 536
SsiI CCGC 2 cut(s) 116, 161
StyD4I CCNGG 1 cut(s) 487
TaiI ACGT 1 cut(s) 503
TaqII GACCGA 1 cut(s) 107
TasI AATT 5 cut(s) 27, 52, 246, 522, 536
Tru1I TTAA 3 cut(s) 239, 468, 540
Tru9I TTAA 3 cut(s) 239, 468, 540
TscAI CASTG 2 cut(s) 403, 409
TseFI GTSAC 1 cut(s) 408
TseI GCWGC 5 cut(s) 8, 15, 560, 563, 566
Tsp45I GTSAC 1 cut(s) 408
TspDTI ATGAA 2 cut(s) 17, 156
TspRI CASTG 2 cut(s) 403, 409
VspI ATTAAT 1 cut(s) 468
XapI RAATTY 3 cut(s) 246, 522, 536
XceI RCATGY 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.