Rmu_sc0006269.1_g000023

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006269.1
OG0013640
Physical Location & Seq
Forward (+)
96844 .. 100302
3459 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006269.1_g000023.1.cds

Sequence Viewer

Length: 771 bp
atgagctttaaaccaggactctcggtcgaattgaggattatcaagattcacagggtcaccagaaaagaaatcgggaaccaccacgaaaaatccagttgcaacaactttatccacaggtttcctttttcaaaagacaatgtgaagtacagggcgctccggcgtcgttttccagaccaagggggactagactttgatctcgggttgcgttgtggtcgagcccggttttggttctttcgggctgcgttgtggtcgacgacggtggtcagcatcaggcttttgggggaggctcttcctgcatcatcggtttccagtcggagtgaatcgacgctttggtgggcggcgagatggctgccacggtctcctgcctggggaggccgccggactgatttggcggtggcgggctgcttctcggaagggccagatttggcggctgggaagagaaaaagtattgtgattgaggagttagggttaggtgaagatgacaatgcttatcatataacgcatactgaattagctaatgacaaggatgacagtgataatgacaattcagatggagactatcatcctgaaattgattttgttgatagtgattacagtggggatgatgattctaacgaagttggttataatgatggtacaacaagtagtattgatgctgaagctactgccaaagatggtggaaatactcgtgtcaatgcaggtgttaatgatggtgaaactaattgtgggagtgatgcaaatgttacaaataattttagtcataatatttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBD_Tnp_Mut MULE SWIM

Protein Analysis

256

Amino Acids

28.32

Weight (kDa)

5.27

Isoelectric Point (pI)

48.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 627
AarI CACCTGC 1 cut(s) 689
Acc36I ACCTGC 1 cut(s) 689
AccI GTMKAC 1 cut(s) 251
AciI CCGC 5 cut(s) 338, 376, 392, 398, 428
AcuI CTGAAG 1 cut(s) 678
AcyI GRCGYC 1 cut(s) 160
AfaI GTAC 2 cut(s) 146, 637
AfiI CCNNNNNNNGG 3 cut(s) 176, 225, 368
AgsI TTSAA 1 cut(s) 129
AhdI GACNNNNNGTC 1 cut(s) 23
AjnI CCWGG 2 cut(s) 13, 365
AluBI AGCT 3 cut(s) 6, 515, 662
AluI AGCT 3 cut(s) 6, 515, 662
Alw26I GTCTC 2 cut(s) 363, 549
Ama87I CYCGRG 1 cut(s) 197
AoxI GGCC 2 cut(s) 373, 416
ApeKI GCWGC 3 cut(s) 239, 349, 402
AspLEI GCGC 1 cut(s) 154
AspS9I GGNCC 1 cut(s) 416
AsuC2I CCSGG 1 cut(s) 220
AsuHPI GGTGA 3 cut(s) 49, 485, 725
AvaI CYCGRG 1 cut(s) 197
BanII GRGCYC 1 cut(s) 220
BauI CACGAG 1 cut(s) 687
BbvI GCAGC 3 cut(s) 226, 336, 389
BccI CCATC 5 cut(s) 339, 545, 626, 668, 704
BcgI CGANNNNNNTGC 2 cut(s) 331, 365
BciT130I CCWGG 2 cut(s) 15, 367
BcnI CCSGG 1 cut(s) 220
BcoDI GTCTC 2 cut(s) 363, 549
BfaI CTAG 1 cut(s) 185
BfoI RGCGCY 1 cut(s) 155
BfuAI ACCTGC 1 cut(s) 689
BisI GCNGC 6 cut(s) 240, 339, 350, 376, 403, 429
BlsI GCNGC 6 cut(s) 241, 340, 351, 377, 404, 430
Bme1390I CCNGG 3 cut(s) 15, 220, 367
BmeRI GACNNNNNGTC 1 cut(s) 23
BmeT110I CYCGRG 1 cut(s) 197
BmgT120I GGNCC 1 cut(s) 416
BmiI GGNNCC 1 cut(s) 77
BmrFI CCNGG 3 cut(s) 15, 220, 367
BmsI GCATC 4 cut(s) 276, 305, 643, 724
BoxI GACNNNNGTC 1 cut(s) 260
BpuMI CCSGG 1 cut(s) 220
BsaHI GRCGYC 1 cut(s) 160
BsaI GGTCTC 1 cut(s) 363
BsaJI CCNNGG 3 cut(s) 175, 353, 366
BsaXI ACNNNNNCTCC 2 cut(s) 452, 482
Bsc4I CCNNNNNNNGG 3 cut(s) 176, 225, 368
Bse1I ACTGG 2 cut(s) 93, 309
BseBI CCWGG 2 cut(s) 15, 367
BseDI CCNNGG 3 cut(s) 175, 353, 366
BseGI GGATG 3 cut(s) 532, 562, 607
BseLI CCNNNNNNNGG 3 cut(s) 176, 225, 368
BseNI ACTGG 2 cut(s) 93, 309
BseRI GAGGAG 1 cut(s) 473
BseXI GCAGC 3 cut(s) 226, 336, 389
BseYI CCCAGC 1 cut(s) 431
Bsh1285I CGRYCG 1 cut(s) 27
BshFI GGCC 2 cut(s) 375, 418
BsiEI CGRYCG 1 cut(s) 27
BsiHKCI CYCGRG 1 cut(s) 197
BsiSI CCGG 3 cut(s) 157, 220, 379
BslFI GGGAC 1 cut(s) 195
BslI CCNNNNNNNGG 3 cut(s) 176, 225, 368
BsmAI GTCTC 2 cut(s) 363, 549
BsmFI GGGAC 1 cut(s) 195
BsnI GGCC 2 cut(s) 375, 418
Bso31I GGTCTC 1 cut(s) 363
BsoBI CYCGRG 1 cut(s) 197
Bsp1286I GDGCHC 1 cut(s) 220
Bsp143I GATC 1 cut(s) 193
BspACI CCGC 5 cut(s) 338, 376, 392, 398, 428
BspANI GGCC 2 cut(s) 375, 418
BspLI GGNNCC 1 cut(s) 77
BspMI ACCTGC 1 cut(s) 689
BspQI GCTCTTC 1 cut(s) 294
BspTNI GGTCTC 1 cut(s) 363
BsrI ACTGG 2 cut(s) 93, 309
BssECI CCNNGG 3 cut(s) 175, 353, 366
BssMI GATC 1 cut(s) 193
BssNI GRCGYC 1 cut(s) 160
BssSI CACGAG 1 cut(s) 687
BssT1I CCWWGG 1 cut(s) 175
Bst2BI CACGAG 1 cut(s) 687
Bst2UI CCWGG 2 cut(s) 15, 367
Bst4CI ACNGT 4 cut(s) 259, 357, 533, 596
Bst6I CTCTTC 2 cut(s) 294, 431
BstACI GRCGYC 1 cut(s) 160
BstC8I GCNNGC 1 cut(s) 400
BstDSI CCRYGG 1 cut(s) 353
BstEII GGTNACC 1 cut(s) 55
BstF5I GGATG 3 cut(s) 532, 562, 607
BstH2I RGCGCY 1 cut(s) 155
BstHHI GCGC 1 cut(s) 154
BstKTI GATC 1 cut(s) 196
BstMAI GTCTC 2 cut(s) 363, 549
BstMBI GATC 1 cut(s) 193
BstMCI CGRYCG 1 cut(s) 27
BstMWI GCNNNNNNNGC 1 cut(s) 293
BstNI CCWGG 2 cut(s) 15, 367
BstPAI GACNNNNGTC 1 cut(s) 260
BstPI GGTNACC 1 cut(s) 55
BstSCI CCNGG 3 cut(s) 13, 218, 365
BstV1I GCAGC 3 cut(s) 226, 336, 389
BsuRI GGCC 2 cut(s) 375, 418
BtgI CCRYGG 1 cut(s) 353
BtsCI GGATG 3 cut(s) 532, 562, 607
BtsIMutI CAGTG 2 cut(s) 538, 601
BveI ACCTGC 1 cut(s) 689
Cac8I GCNNGC 1 cut(s) 400
CfoI GCGC 1 cut(s) 154
Cfr13I GGNCC 1 cut(s) 416
CseI GACGC 2 cut(s) 149, 334
Csp6I GTAC 2 cut(s) 145, 636
CspCI CAANNNNNGTGG 2 cut(s) 658, 693
CviQI GTAC 2 cut(s) 145, 636
DpnI GATC 1 cut(s) 195
DpnII GATC 1 cut(s) 193
DraI TTTAAA 1 cut(s) 10
DriI GACNNNNNGTC 1 cut(s) 23
Eam1104I CTCTTC 2 cut(s) 294, 431
Eam1105I GACNNNNNGTC 1 cut(s) 23
EarI CTCTTC 2 cut(s) 294, 431
Eco130I CCWWGG 1 cut(s) 175
Eco24I GRGCYC 1 cut(s) 220
Eco31I GGTCTC 1 cut(s) 363
Eco57I CTGAAG 1 cut(s) 678
Eco88I CYCGRG 1 cut(s) 197
Eco91I GGTNACC 1 cut(s) 55
EcoO65I GGTNACC 1 cut(s) 55
EcoRII CCWGG 2 cut(s) 13, 365
EcoT14I CCWWGG 1 cut(s) 175
EcoT38I GRGCYC 1 cut(s) 220
ErhI CCWWGG 1 cut(s) 175
FaiI YATR 5 cut(s) 495, 497, 504, 627, 762
FaqI GGGAC 1 cut(s) 195
FauI CCCGC 1 cut(s) 391
FblI GTMKAC 1 cut(s) 251
Fnu4HI GCNGC 6 cut(s) 240, 339, 350, 376, 403, 429
FokI GGATG 3 cut(s) 539, 549, 614
FriOI GRGCYC 1 cut(s) 220
Fsp4HI GCNGC 6 cut(s) 240, 339, 350, 376, 403, 429
FspBI CTAG 1 cut(s) 185
GlaI GCGC 1 cut(s) 153
GluI GCNGC 6 cut(s) 240, 339, 350, 376, 403, 429
GsaI CCCAGC 1 cut(s) 435
HaeII RGCGCY 1 cut(s) 155
HaeIII GGCC 2 cut(s) 375, 418
HapII CCGG 3 cut(s) 157, 220, 379
HgaI GACGC 2 cut(s) 149, 334
HhaI GCGC 1 cut(s) 154
Hin1I GRCGYC 1 cut(s) 160
Hin6I GCGC 1 cut(s) 152
HinP1I GCGC 1 cut(s) 152
HincII GTYRAC 1 cut(s) 252
HindII GTYRAC 1 cut(s) 252
HinfI GANTC 4 cut(s) 18, 46, 320, 608
HpaII CCGG 3 cut(s) 157, 220, 379
HphI GGTGA 3 cut(s) 49, 485, 725
Hpy166II GTNNAC 1 cut(s) 252
Hpy188I TCNGA 3 cut(s) 315, 412, 550
Hpy188III TCNNGA 4 cut(s) 43, 73, 170, 566
Hpy8I GTNNAC 1 cut(s) 252
Hpy99I CGWCG 4 cut(s) 165, 256, 259, 328
HpyAV CCTTC 1 cut(s) 407
HpyCH4III ACNGT 4 cut(s) 259, 357, 533, 596
HpyCH4V TGCA 4 cut(s) 99, 296, 698, 737
HpyF10VI GCNNNNNNNGC 1 cut(s) 293
Hsp92I GRCGYC 1 cut(s) 160
HspAI GCGC 1 cut(s) 152
Kzo9I GATC 1 cut(s) 193
LguI GCTCTTC 1 cut(s) 294
LmnI GCTCC 1 cut(s) 159
Lsp1109I GCAGC 3 cut(s) 226, 336, 389
LweI GCATC 4 cut(s) 276, 305, 643, 724
MaeI CTAG 1 cut(s) 185
MaeIII GTNAC 2 cut(s) 55, 742
MalI GATC 1 cut(s) 195
MboI GATC 1 cut(s) 193
MboII GAAGA 3 cut(s) 281, 448, 488
MhlI GDGCHC 1 cut(s) 220
MluCI AATT 6 cut(s) 29, 509, 544, 570, 721, 751
MlyI GAGTC 1 cut(s) 12
MmeI TCCRAC 1 cut(s) 293
MnlI CCTC 4 cut(s) 27, 277, 365, 451
MseI TTAA 3 cut(s) 9, 705, 769
MspI CCGG 3 cut(s) 157, 220, 379
MspR9I CCNGG 3 cut(s) 15, 220, 367
MvaI CCWGG 2 cut(s) 15, 367
MwoI GCNNNNNNNGC 1 cut(s) 293
NciI CCSGG 1 cut(s) 220
NdeII GATC 1 cut(s) 193
NlaIV GGNNCC 1 cut(s) 77
NmuCI GTSAC 1 cut(s) 55
PaqCI CACCTGC 1 cut(s) 689
PciSI GCTCTTC 1 cut(s) 294
PfeI GAWTC 3 cut(s) 46, 320, 608
PkrI GCNGC 6 cut(s) 241, 340, 351, 377, 404, 430
PleI GAGTC 1 cut(s) 12
PpsI GAGTC 1 cut(s) 12
PshAI GACNNNNGTC 1 cut(s) 260
PsiI TTATAA 1 cut(s) 627
Psp6I CCWGG 2 cut(s) 13, 365
PspEI GGTNACC 1 cut(s) 55
PspFI CCCAGC 1 cut(s) 431
PspGI CCWGG 2 cut(s) 13, 365
PspN4I GGNNCC 1 cut(s) 77
PspPI GGNCC 1 cut(s) 416
RsaI GTAC 2 cut(s) 146, 637
RsaNI GTAC 2 cut(s) 145, 636
SalI GTCGAC 1 cut(s) 250
SapI GCTCTTC 1 cut(s) 294
SaqAI TTAA 3 cut(s) 9, 705, 769
SatI GCNGC 6 cut(s) 240, 339, 350, 376, 403, 429
Sau3AI GATC 1 cut(s) 193
Sau96I GGNCC 1 cut(s) 416
SchI GAGTC 1 cut(s) 12
ScrFI CCNGG 3 cut(s) 15, 220, 367
SduI GDGCHC 1 cut(s) 220
SetI ASST 6 cut(s) 8, 119, 475, 517, 664, 703
SfaNI GCATC 4 cut(s) 276, 305, 643, 724
Sse9I AATT 6 cut(s) 29, 509, 544, 570, 721, 751
SsiI CCGC 5 cut(s) 338, 376, 392, 398, 428
SspI AATATT 1 cut(s) 766
SspMI CTAG 1 cut(s) 185
StyD4I CCNGG 3 cut(s) 13, 218, 365
StyI CCWWGG 1 cut(s) 175
TaaI ACNGT 4 cut(s) 259, 357, 533, 596
TaqI TCGA 4 cut(s) 27, 214, 251, 323
TaqII GACCGA 1 cut(s) 13
TasI AATT 6 cut(s) 29, 509, 544, 570, 721, 751
TatI WGTACW 1 cut(s) 144
TauI GCSGC 3 cut(s) 341, 378, 431
TfiI GAWTC 3 cut(s) 46, 320, 608
Tru1I TTAA 3 cut(s) 9, 705, 769
Tru9I TTAA 3 cut(s) 9, 705, 769
TscAI CASTG 2 cut(s) 538, 601
TseFI GTSAC 1 cut(s) 55
TseI GCWGC 3 cut(s) 239, 349, 402
Tsp45I GTSAC 1 cut(s) 55
TspRI CASTG 2 cut(s) 538, 601
XmiI GTMKAC 1 cut(s) 251
XspI CTAG 1 cut(s) 185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.