Rh4AG115200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0013640
Physical Location & Seq
Forward (+)
24865350 .. 24876102
10753 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG115200.1

Sequence Viewer

Length: 990 bp
ATGTATCACAATGGTGATATGCAACCGCTGTGCTATGTAAATGGGGATATTGCTCACTTTGACTATTGTGACAAGGATAATATGTCTATGTGTGAGATTATTGCAATGGGAAAAGAATTAGGTCATCATGGCATATTAAATTATTGGTATAAGTTGCCTGGGATGGGAATTGGAGCTGCCATGAGTTTGTTAAAAAGGGATGATGATGTTGTTGATATGGTGGCTTTTGTGCCAGATGTGAGGATAATTGAAGTGTACTTGGAGCATATACTAGACTCTGGTCCTTGTGACATTGAAAACTTAGGTTGGGACCTCAATGACTTTGGAATAGACCTCAGTAAAAGTTCTGATGTGGGAGCTAGGAAGAGAAAAAGTATTGTGATTGAGGAGTTAGGGTTAGGTGAAGATGACAATGCTTATCATATAACGCAGACTAAATTAGCTAATGACAAGAATGACAGTGATAATGACAATTTAGATGGAGACTATCATCCTGAAGCTGATTTTGTTGATAGTGATTACAGTGGGGATGATGATAATTCTGACGAAGTTGGTTATAATGATGGTACAGCAAGTAGTATTGATGCTGAAGCTACTGCCAAAGATGGTGGAAATACTCGTGTCAATGCAGGTGTTGATGATGGACACAATCTGAGATCATGTCACAGACATCTTCCTCCGAAAAAAAAGCCTGCTCCAAAACCTAGAGGAAGGCCAAGGAAAAATCCACAAATCAGTGCAACTGCTACATTGCAACCAATAACAACTTCAGTTCCTCCATGTACATCTGCAAGAACAACTACACCACTTTCCTCAACTTCTACAAGAATTGCTCCACTAACACCATCCACTACTCTAGCAGCTCCGCAACGTAATGTTAGTAGAAAATCATCAACGACCTCTACTCAGAAAATCAGCAACAGGCATAAACATACATTTACAAAGCAATATATCGATCCCAAGCTCTTCTCAAAAGAGCCAATGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBD_Tnp_Mut MULE SWIM

Protein Analysis

329

Amino Acids

36.14

Weight (kDa)

5.33

Isoelectric Point (pI)

41.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1-like PF26130 7 - 89 1.6e-12 PB1-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 558
AarI CACCTGC 1 cut(s) 620
Acc36I ACCTGC 1 cut(s) 620
AciI CCGC 2 cut(s) 26, 866
AclWI GGATC 1 cut(s) 950
AcuI CTGAAG 3 cut(s) 516, 609, 753
AfaI GTAC 3 cut(s) 257, 568, 784
AfiI CCNNNNNNNGG 1 cut(s) 164
AgsI TTSAA 2 cut(s) 251, 296
AjnI CCWGG 1 cut(s) 157
AjuI GAANNNNNNNTTGG 2 cut(s) 751, 783
AleI CACNNNNGTG 1 cut(s) 12
AluBI AGCT 7 cut(s) 176, 359, 443, 500, 593, 863, 964
AluI AGCT 7 cut(s) 176, 359, 443, 500, 593, 863, 964
Alw26I GTCTC 1 cut(s) 477
AlwI GGATC 1 cut(s) 950
AoxI GGCC 1 cut(s) 713
ApeKI GCWGC 2 cut(s) 176, 860
AspS9I GGNCC 2 cut(s) 281, 310
AsuHPI GGTGA 2 cut(s) 26, 413
AvaII GGWCC 2 cut(s) 281, 310
BauI CACGAG 1 cut(s) 618
BbvI GCAGC 2 cut(s) 163, 872
BccI CCATC 6 cut(s) 157, 473, 557, 599, 635, 853
BciT130I CCWGG 1 cut(s) 159
BcoDI GTCTC 1 cut(s) 477
BfaI CTAG 4 cut(s) 272, 360, 705, 857
BfuAI ACCTGC 1 cut(s) 620
BisI GCNGC 2 cut(s) 177, 861
BlsI GCNGC 2 cut(s) 178, 862
Bme1390I CCNGG 1 cut(s) 159
Bme18I GGWCC 2 cut(s) 281, 310
BmgT120I GGNCC 2 cut(s) 281, 310
BmiI GGNNCC 1 cut(s) 311
BmrFI CCNGG 1 cut(s) 159
BmsI GCATC 1 cut(s) 574
BoxI GACNNNNGTC 1 cut(s) 279
Bsa29I ATCGAT 1 cut(s) 954
BsaJI CCNNGG 2 cut(s) 158, 716
BsaXI ACNNNNNCTCC 2 cut(s) 380, 410
Bsc4I CCNNNNNNNGG 1 cut(s) 164
Bse3DI GCAATG 2 cut(s) 111, 749
BseBI CCWGG 1 cut(s) 159
BseCI ATCGAT 1 cut(s) 954
BseDI CCNNGG 2 cut(s) 158, 716
BseGI GGATG 5 cut(s) 168, 205, 490, 535, 845
BseLI CCNNNNNNNGG 1 cut(s) 164
BseMI GCAATG 2 cut(s) 111, 749
BseMII CTCAG 3 cut(s) 349, 644, 920
BseRI GAGGAG 1 cut(s) 401
BseXI GCAGC 2 cut(s) 163, 872
BshFI GGCC 1 cut(s) 715
BshVI ATCGAT 1 cut(s) 954
BslFI GGGAC 1 cut(s) 323
BslI CCNNNNNNNGG 1 cut(s) 164
BsmAI GTCTC 1 cut(s) 477
BsmFI GGGAC 1 cut(s) 323
BsnI GGCC 1 cut(s) 715
Bsp1407I TGTACA 1 cut(s) 782
Bsp143I GATC 2 cut(s) 656, 955
BspACI CCGC 2 cut(s) 26, 866
BspANI GGCC 1 cut(s) 715
BspCNI CTCAG 3 cut(s) 348, 645, 919
BspDI ATCGAT 1 cut(s) 954
BspLI GGNNCC 1 cut(s) 311
BspMI ACCTGC 1 cut(s) 620
BspPI GGATC 1 cut(s) 950
BspQI GCTCTTC 1 cut(s) 971
BsrDI GCAATG 2 cut(s) 111, 749
BsrGI TGTACA 1 cut(s) 782
BssECI CCNNGG 2 cut(s) 158, 716
BssMI GATC 2 cut(s) 656, 955
BssSI CACGAG 1 cut(s) 618
BssT1I CCWWGG 1 cut(s) 716
Bst2BI CACGAG 1 cut(s) 618
Bst2UI CCWGG 1 cut(s) 159
Bst4CI ACNGT 2 cut(s) 461, 524
Bst6I CTCTTC 2 cut(s) 359, 971
BstAUI TGTACA 1 cut(s) 782
BstC8I GCNNGC 1 cut(s) 693
BstDEI CTNAG 4 cut(s) 301, 335, 653, 906
BstF5I GGATG 5 cut(s) 168, 205, 490, 535, 845
BstKTI GATC 2 cut(s) 659, 958
BstMAI GTCTC 1 cut(s) 477
BstMBI GATC 2 cut(s) 656, 955
BstNI CCWGG 1 cut(s) 159
BstPAI GACNNNNGTC 1 cut(s) 279
BstSCI CCNGG 1 cut(s) 157
BstV1I GCAGC 2 cut(s) 163, 872
Bsu15I ATCGAT 1 cut(s) 954
BsuRI GGCC 1 cut(s) 715
BsuTUI ATCGAT 1 cut(s) 954
BtsCI GGATG 5 cut(s) 168, 205, 490, 535, 845
BtsIMutI CAGTG 3 cut(s) 466, 529, 742
BveI ACCTGC 1 cut(s) 620
Cac8I GCNNGC 1 cut(s) 693
Cfr13I GGNCC 2 cut(s) 281, 310
ClaI ATCGAT 1 cut(s) 954
Csp6I GTAC 3 cut(s) 256, 567, 783
CspCI CAANNNNNGTGG 2 cut(s) 589, 624
CviAII CATG 4 cut(s) 128, 181, 660, 780
CviQI GTAC 3 cut(s) 256, 567, 783
DdeI CTNAG 4 cut(s) 301, 335, 653, 906
DpnI GATC 2 cut(s) 658, 957
DpnII GATC 2 cut(s) 656, 955
Eam1104I CTCTTC 2 cut(s) 359, 971
EarI CTCTTC 2 cut(s) 359, 971
Eco130I CCWWGG 1 cut(s) 716
Eco47I GGWCC 2 cut(s) 281, 310
Eco57I CTGAAG 3 cut(s) 516, 609, 753
EcoO109I RGGNCCY 1 cut(s) 310
EcoRII CCWGG 1 cut(s) 157
EcoT14I CCWWGG 1 cut(s) 716
ErhI CCWWGG 1 cut(s) 716
FaeI CATG 4 cut(s) 131, 184, 663, 783
FaqI GGGAC 1 cut(s) 323
FatI CATG 4 cut(s) 127, 180, 659, 779
Fnu4HI GCNGC 2 cut(s) 177, 861
FokI GGATG 5 cut(s) 175, 212, 477, 542, 832
Fsp4HI GCNGC 2 cut(s) 177, 861
FspBI CTAG 4 cut(s) 272, 360, 705, 857
GluI GCNGC 2 cut(s) 177, 861
HaeIII GGCC 1 cut(s) 715
Hin1II CATG 4 cut(s) 131, 184, 663, 783
HinfI GANTC 1 cut(s) 275
HphI GGTGA 2 cut(s) 26, 413
Hpy166II GTNNAC 1 cut(s) 256
Hpy188I TCNGA 5 cut(s) 349, 544, 654, 681, 909
Hpy188III TCNNGA 1 cut(s) 494
Hpy8I GTNNAC 1 cut(s) 256
HpyAV CCTTC 1 cut(s) 705
HpyCH4III ACNGT 2 cut(s) 461, 524
HpyCH4IV ACGT 1 cut(s) 871
HpyCH4V TGCA 6 cut(s) 22, 104, 629, 740, 754, 791
HpyF3I CTNAG 4 cut(s) 301, 335, 653, 906
HpySE526I ACGT 1 cut(s) 871
Hsp92II CATG 4 cut(s) 131, 184, 663, 783
Kzo9I GATC 2 cut(s) 656, 955
LguI GCTCTTC 1 cut(s) 971
LmnI GCTCC 6 cut(s) 173, 262, 356, 700, 838, 868
LpnPI CCDG 8 cut(s) 144, 171, 246, 264, 507, 615, 705, 907
Lsp1109I GCAGC 2 cut(s) 163, 872
LweI GCATC 1 cut(s) 574
MaeI CTAG 4 cut(s) 272, 360, 705, 857
MaeII ACGT 1 cut(s) 871
MaeIII GTNAC 3 cut(s) 68, 287, 662
MalI GATC 2 cut(s) 658, 957
MboI GATC 2 cut(s) 656, 955
MboII GAAGA 4 cut(s) 376, 416, 665, 958
MluCI AATT 8 cut(s) 116, 139, 168, 246, 437, 472, 538, 828
MlyI GAGTC 1 cut(s) 269
MnlI CCTC 9 cut(s) 234, 323, 344, 379, 687, 701, 786, 823, 910
MseI TTAA 2 cut(s) 137, 191
MslI CAYNNNNRTG 1 cut(s) 12
MspA1I CMGCKG 1 cut(s) 28
MspR9I CCNGG 1 cut(s) 159
MvaI CCWGG 1 cut(s) 159
NdeII GATC 2 cut(s) 656, 955
NlaIII CATG 4 cut(s) 131, 184, 663, 783
NlaIV GGNNCC 1 cut(s) 311
NmuCI GTSAC 3 cut(s) 68, 287, 662
OliI CACNNNNGTG 1 cut(s) 12
PaqCI CACCTGC 1 cut(s) 620
PciSI GCTCTTC 1 cut(s) 971
PkrI GCNGC 2 cut(s) 178, 862
PleI GAGTC 1 cut(s) 269
PpsI GAGTC 1 cut(s) 269
PpuMI RGGWCCY 1 cut(s) 310
PshAI GACNNNNGTC 1 cut(s) 279
PsiI TTATAA 1 cut(s) 558
Psp5II RGGWCCY 1 cut(s) 310
Psp6I CCWGG 1 cut(s) 157
PspGI CCWGG 1 cut(s) 157
PspN4I GGNNCC 1 cut(s) 311
PspPI GGNCC 2 cut(s) 281, 310
PspPPI RGGWCCY 1 cut(s) 310
RsaI GTAC 3 cut(s) 257, 568, 784
RsaNI GTAC 3 cut(s) 256, 567, 783
RseI CAYNNNNRTG 1 cut(s) 12
SapI GCTCTTC 1 cut(s) 971
SaqAI TTAA 2 cut(s) 137, 191
SatI GCNGC 2 cut(s) 177, 861
Sau3AI GATC 2 cut(s) 656, 955
Sau96I GGNCC 2 cut(s) 281, 310
SchI GAGTC 1 cut(s) 269
ScrFI CCNGG 1 cut(s) 159
SfaNI GCATC 1 cut(s) 574
SinI GGWCC 2 cut(s) 281, 310
SmiMI CAYNNNNRTG 1 cut(s) 12
Sse9I AATT 8 cut(s) 116, 139, 168, 246, 437, 472, 538, 828
SsiI CCGC 2 cut(s) 26, 866
SspMI CTAG 4 cut(s) 272, 360, 705, 857
StyD4I CCNGG 1 cut(s) 157
StyI CCWWGG 1 cut(s) 716
TaaI ACNGT 2 cut(s) 461, 524
TaiI ACGT 1 cut(s) 874
TaqI TCGA 1 cut(s) 954
TasI AATT 8 cut(s) 116, 139, 168, 246, 437, 472, 538, 828
TatI WGTACW 2 cut(s) 255, 782
Tru1I TTAA 2 cut(s) 137, 191
Tru9I TTAA 2 cut(s) 137, 191
TscAI CASTG 3 cut(s) 466, 529, 742
TseFI GTSAC 3 cut(s) 68, 287, 662
TseI GCWGC 2 cut(s) 176, 860
Tsp45I GTSAC 3 cut(s) 68, 287, 662
TspRI CASTG 3 cut(s) 466, 529, 742
VpaK11BI GGWCC 2 cut(s) 281, 310
XspI CTAG 4 cut(s) 272, 360, 705, 857
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.