Rmu_sc0006366.1_g000006

Non-catalytic subunit of the queuine tRNA- ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, - His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7- deazaguanosine)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006366.1
OG0006935
Physical Location & Seq
Forward (+)
20717 .. 31792
11076 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2034 bp
atgaaattcgcagtgaaggcatggagcagtggaagagcacgggcaggggtactccagctgggcagctgcccgaacccaatagagacccctgctcttctcctctctacccgaaagggcctgccccttttcataccccctgaccttctcccttctcttccttcccccgactcccatctcctccatcttagctcccttcacttcttagaaggcatctcaccccagacaatatcaaaaataggaggattgcataagatgctgggtttgccggaccacggatttgtagccattccgagagattctattcaatgcctccctgaatgtaatagcaccaataaattgggagcatctttcgacactccctgtggtcgcagtttggttaagcctgtagagtatgttgaattgattttttcgttaaagcctaatatatggaccgctttggcggatgaagttcctgcatgggtgtctgataagaggaacaagacctctgttgatcgaactgtaagatggctcgacgaatgcattcagctaaatcgggcaggtggatctgtttttggatccattgttggaggatcttctgttctagaacgtgagcgatgtgcgcaagaggtggctagtcgaagtgtatcaggctattggattggaggttttgggctgggagagagcatggatgagcgccctgctctccttaatgctgttactgatattttagaaggggagaaaccacgcttggtcagtgggcttggacttccagaggaggtcttgcagggtgttgctgcaggaattgatatctttgactctacgtatatataccatcttaccgttggaggctttgcacttaccttcccactagatggtgttgagaatgtctctgcttttcagttaactaatataggaagtgatcaaacaaagattaatttgagagcaacaatttacaggaaagacacttcaccaattgctcaaaactgtaaatgttacacatgccagaatcatacaaaagcgtacatcaatcacctgctcaatgttcatgaaatgctagctcagattctacttgaaatacataatactcaccactatctaagtttcttccgctcagtaagggaggcaattaagtctggtaattttgagcaattcagactgaaattttctgagagtaagcgttatcatcttgcttctgaagctgttgttgcctgcataatattgaactacatccaaccctgcttacaagtggctggaacaaaacatttgaaaccacatgttctcatgctctcctcactaattgaatgttttatcatcactcctgtatctgatatcttgatgtggaaatggtttgagaatcaccttctgaaggagctgctgaccagcattcagacatgcatggagcatgctaactgcttgatcaaaaggagtctgagcctgttggggttgggtccttggaatgttgtgtccaataagaaacgagggaataagattcataaacgtgtttctgtacaggatgctgagggtagtgcaataggttctcctctaaggatgaaggggaactggggatatgttctgctccccttgttagttaatgtctttaaggttcactaccgatggaagaggtctagtgatcaaaatctaggtactggcggtgttttaagagattctactggtctttggttggcgtcaaggtttttttggctagtttcggctttactgggtcaagtagaactatggggtgtgttcaatcggctgtacgcgcaccacgcgcttgccttgattcccttctattctatttttcgtctcttctactctacctacttcgacccacctactgttgttgttactccattgacagaacggcgaccgcgacgacgcttctccgctactccatcggtggcggtggagcctcgatctactccaccatctctcttcaaacgtgttcggtacgacgattcgatctatccctttcgctactccatcggtggcggtggagcctcgatctactccaccatctctcttcaaacgtgttcggtacgacgattcgatctatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TGT

Protein Analysis

677

Amino Acids

75.47

Weight (kDa)

9.19

Isoelectric Point (pI)

50.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TGT PF01702 12 - 384 5.8e-86 Queuine tRNA-ribosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 527, 1020
Acc16I TGCGCA 1 cut(s) 600
Acc36I ACCTGC 2 cut(s) 527, 1020
AccB7I CCANNNNNTGG 1 cut(s) 851
AccBSI CCGCTC 1 cut(s) 1089
AccII CGCG 3 cut(s) 1739, 1748, 1849
AciI CCGC 8 cut(s) 432, 440, 1087, 1629, 1847, 1863, 1880, 1968
AclWI GGATC 4 cut(s) 549, 550, 562, 577
AcsI RAATTY 2 cut(s) 5, 1139
AcuI CTGAAG 2 cut(s) 1194, 1364
AcyI GRCGYC 1 cut(s) 1664
AfaI GTAC 7 cut(s) 51, 1001, 1488, 1624, 1736, 1928, 2016
AfiI CCNNNNNNNGG 3 cut(s) 272, 851, 1345
AflIII ACRYGT 4 cut(s) 1252, 1477, 1918, 2006
AgsI TTSAA 9 cut(s) 305, 398, 1052, 1201, 1246, 1280, 1726, 1915, 2003
AjuI GAANNNNNNNTTGG 2 cut(s) 710, 742
AloI GAACNNNNNNTCC 2 cut(s) 561, 593
AluBI AGCT 7 cut(s) 58, 66, 189, 526, 1037, 1178, 1351
AluI AGCT 7 cut(s) 58, 66, 189, 526, 1037, 1178, 1351
Alw21I GWGCWC 1 cut(s) 40
Alw26I GTCTC 3 cut(s) 77, 871, 1787
AlwI GGATC 4 cut(s) 549, 550, 562, 577
AoxI GGCC 1 cut(s) 115
ApeKI GCWGC 4 cut(s) 63, 66, 773, 1351
ApoI RAATTY 2 cut(s) 5, 1139
ArsI GACNNNNNNTTYG 2 cut(s) 260, 292
AseI ATTAAT 1 cut(s) 912
Asp700I GAANNNNTTC 1 cut(s) 519
AspLEI GCGC 4 cut(s) 601, 675, 1741, 1750
AspS9I GGNCC 4 cut(s) 115, 268, 429, 1427
AsuHPI GGTGA 5 cut(s) 207, 939, 1001, 1058, 1328
AsuNHI GCTAGC 1 cut(s) 1033
AvaII GGWCC 3 cut(s) 268, 429, 1427
BaeI ACNNNNGTAYC 2 cut(s) 1284, 1317
BamHI GGATCC 1 cut(s) 554
Bbv12I GWGCWC 1 cut(s) 40
BbvCI CCTCAGC 1 cut(s) 1497
BbvI GCAGC 4 cut(s) 53, 75, 760, 1338
BceAI ACGGC 1 cut(s) 1856
BclI TGATCA 3 cut(s) 898, 1395, 1609
BcoDI GTCTC 3 cut(s) 77, 871, 1787
BfaI CTAG 7 cut(s) 581, 612, 848, 1034, 1605, 1619, 1682
BfmI CTRYAG 2 cut(s) 384, 774
BfoI RGCGCY 1 cut(s) 676
BfuAI ACCTGC 2 cut(s) 527, 1020
BisI GCNGC 4 cut(s) 64, 67, 774, 1352
BlsI GCNGC 4 cut(s) 65, 68, 775, 1353
Bme18I GGWCC 3 cut(s) 268, 429, 1427
BmgT120I GGNCC 4 cut(s) 115, 268, 429, 1427
BmiI GGNNCC 4 cut(s) 556, 1428, 1887, 1975
BmrI ACTGGG 2 cut(s) 1549, 1706
BmsI GCATC 4 cut(s) 219, 243, 353, 1483
BmtI GCTAGC 1 cut(s) 1037
BmuI ACTGGG 2 cut(s) 1549, 1706
BplI GAGNNNNNCTC 2 cut(s) 851, 883
BpmI CTGGAG 1 cut(s) 38
Bpu10I CCTNAGC 1 cut(s) 1497
BsaAI YACGTR 1 cut(s) 801
BsaBI GATNNNNATC 1 cut(s) 1614
BsaHI GRCGYC 1 cut(s) 1664
BsaI GGTCTC 1 cut(s) 77
BsaJI CCNNGG 2 cut(s) 271, 1430
Bsc4I CCNNNNNNNGG 3 cut(s) 272, 851, 1345
Bse1I ACTGG 4 cut(s) 1544, 1630, 1654, 1701
Bse8I GATNNNNATC 1 cut(s) 1614
BseDI CCNNGG 2 cut(s) 271, 1430
BseGI GGATG 5 cut(s) 448, 673, 1206, 1498, 1533
BseJI GATNNNNATC 1 cut(s) 1614
BseLI CCNNNNNNNGG 3 cut(s) 272, 851, 1345
BseMII CTCAG 5 cut(s) 1052, 1104, 1137, 1400, 1488
BseNI ACTGG 4 cut(s) 1544, 1630, 1654, 1701
BseRI GAGGAG 5 cut(s) 89, 167, 767, 1258, 1509
BseXI GCAGC 4 cut(s) 53, 75, 760, 1338
BseYI CCCAGC 3 cut(s) 58, 256, 652
Bsh1236I CGCG 3 cut(s) 1739, 1748, 1849
Bsh1285I CGRYCG 1 cut(s) 1847
BshFI GGCC 1 cut(s) 117
BsiEI CGRYCG 1 cut(s) 1847
BsiHKAI GWGCWC 1 cut(s) 40
BsiSI CCGG 1 cut(s) 266
BslI CCNNNNNNNGG 3 cut(s) 272, 851, 1345
BsmAI GTCTC 3 cut(s) 77, 871, 1787
BsmBI CGTCTC 1 cut(s) 1787
BsmI GAATGC 3 cut(s) 519, 521, 1362
BsnI GGCC 1 cut(s) 117
Bso31I GGTCTC 1 cut(s) 77
Bsp1286I GDGCHC 1 cut(s) 40
Bsp1407I TGTACA 1 cut(s) 1486
BspACI CCGC 8 cut(s) 432, 440, 1087, 1629, 1847, 1863, 1880, 1968
BspANI GGCC 1 cut(s) 117
BspCNI CTCAG 5 cut(s) 1051, 1103, 1138, 1401, 1489
BspFNI CGCG 3 cut(s) 1739, 1748, 1849
BspHI TCATGA 1 cut(s) 1024
BspLI GGNNCC 4 cut(s) 556, 1428, 1887, 1975
BspMAI CTGCAG 1 cut(s) 778
BspMI ACCTGC 2 cut(s) 527, 1020
BspOI GCTAGC 1 cut(s) 1037
BspPI GGATC 4 cut(s) 549, 550, 562, 577
BspQI GCTCTTC 2 cut(s) 28, 99
BspTNI GGTCTC 1 cut(s) 77
BsrBI CCGCTC 1 cut(s) 1089
BsrGI TGTACA 1 cut(s) 1486
BsrI ACTGG 4 cut(s) 1544, 1630, 1654, 1701
BssECI CCNNGG 2 cut(s) 271, 1430
BssNI GRCGYC 1 cut(s) 1664
BssT1I CCWWGG 1 cut(s) 1430
Bst4CI ACNGT 4 cut(s) 499, 820, 965, 1816
Bst6I CTCTTC 7 cut(s) 28, 99, 159, 1592, 1790, 1916, 2004
BstACI GRCGYC 1 cut(s) 1664
BstAPI GCANNNNNTGC 1 cut(s) 253
BstAUI TGTACA 1 cut(s) 1486
BstBAI YACGTR 1 cut(s) 801
BstC8I GCNNGC 5 cut(s) 119, 1035, 1189, 1383, 1752
BstDEI CTNAG 9 cut(s) 185, 202, 1038, 1076, 1090, 1146, 1409, 1497, 1523
BstDSI CCRYGG 1 cut(s) 271
BstENI CCTNNNNNAGG 1 cut(s) 1343
BstF5I GGATG 5 cut(s) 448, 673, 1206, 1498, 1533
BstFNI CGCG 3 cut(s) 1739, 1748, 1849
BstH2I RGCGCY 1 cut(s) 676
BstHHI GCGC 4 cut(s) 601, 675, 1741, 1750
BstMAI GTCTC 3 cut(s) 77, 871, 1787
BstMCI CGRYCG 1 cut(s) 1847
BstMWI GCNNNNNNNGC 9 cut(s) 17, 253, 262, 598, 1175, 1184, 1738, 1745, 1747
BstNSI RCATGY 4 cut(s) 981, 1256, 1374, 1385
BstSFI CTRYAG 2 cut(s) 384, 774
BstSNI TACGTA 1 cut(s) 801
BstUI CGCG 3 cut(s) 1739, 1748, 1849
BstV1I GCAGC 4 cut(s) 53, 75, 760, 1338
BstX2I RGATCY 3 cut(s) 542, 554, 569
BstXI CCANNNNNNTGG 1 cut(s) 337
BstYI RGATCY 3 cut(s) 542, 554, 569
BsuRI GGCC 1 cut(s) 117
BtgI CCRYGG 1 cut(s) 271
BtgZI GCGATG 1 cut(s) 607
BtsCI GGATG 5 cut(s) 448, 673, 1206, 1498, 1533
BtsI GCAGTG 2 cut(s) 18, 34
BtsIMutI CAGTG 3 cut(s) 18, 34, 739
BveI ACCTGC 2 cut(s) 527, 1020
Cac8I GCNNGC 5 cut(s) 119, 1035, 1189, 1383, 1752
CciI TCATGA 1 cut(s) 1024
CfoI GCGC 4 cut(s) 601, 675, 1741, 1750
Cfr13I GGNCC 4 cut(s) 115, 268, 429, 1427
CseI GACGC 2 cut(s) 1653, 1863
Csp6I GTAC 7 cut(s) 50, 1000, 1487, 1623, 1735, 1927, 2015
CviQI GTAC 7 cut(s) 50, 1000, 1487, 1623, 1735, 1927, 2015
DdeI CTNAG 9 cut(s) 185, 202, 1038, 1076, 1090, 1146, 1409, 1497, 1523
Eam1104I CTCTTC 7 cut(s) 28, 99, 159, 1592, 1790, 1916, 2004
EarI CTCTTC 7 cut(s) 28, 99, 159, 1592, 1790, 1916, 2004
EciI GGCGGA 1 cut(s) 455
Eco105I TACGTA 1 cut(s) 801
Eco130I CCWWGG 1 cut(s) 1430
Eco31I GGTCTC 1 cut(s) 77
Eco32I GATATC 2 cut(s) 787, 1309
Eco47I GGWCC 3 cut(s) 268, 429, 1427
Eco57I CTGAAG 2 cut(s) 1194, 1364
EcoNI CCTNNNNNAGG 1 cut(s) 1343
EcoO109I RGGNCCY 2 cut(s) 115, 1427
EcoRV GATATC 2 cut(s) 787, 1309
EcoT14I CCWWGG 1 cut(s) 1430
EcoT22I ATGCAT 2 cut(s) 521, 1376
ErhI CCWWGG 1 cut(s) 1430
Esp3I CGTCTC 1 cut(s) 1787
FbaI TGATCA 3 cut(s) 898, 1395, 1609
Fnu4HI GCNGC 4 cut(s) 64, 67, 774, 1352
FokI GGATG 5 cut(s) 455, 680, 1193, 1505, 1540
Fsp4HI GCNGC 4 cut(s) 64, 67, 774, 1352
FspBI CTAG 7 cut(s) 581, 612, 848, 1034, 1605, 1619, 1682
FspI TGCGCA 1 cut(s) 600
GlaI GCGC 4 cut(s) 600, 674, 1740, 1749
GluI GCNGC 4 cut(s) 64, 67, 774, 1352
GsaI CCCAGC 3 cut(s) 62, 260, 656
GsuI CTGGAG 1 cut(s) 38
HaeII RGCGCY 1 cut(s) 676
HaeIII GGCC 1 cut(s) 117
HapII CCGG 1 cut(s) 266
HgaI GACGC 2 cut(s) 1653, 1863
HhaI GCGC 4 cut(s) 601, 675, 1741, 1750
Hin1I GRCGYC 1 cut(s) 1664
Hin6I GCGC 4 cut(s) 599, 673, 1739, 1748
HinP1I GCGC 4 cut(s) 599, 673, 1739, 1748
HincII GTYRAC 1 cut(s) 882
HindII GTYRAC 1 cut(s) 882
HpaI GTTAAC 1 cut(s) 882
HpaII CCGG 1 cut(s) 266
HphI GGTGA 5 cut(s) 207, 939, 1001, 1058, 1328
Hpy166II GTNNAC 2 cut(s) 882, 1585
Hpy188III TCNNGA 4 cut(s) 581, 749, 1025, 1312
Hpy8I GTNNAC 2 cut(s) 882, 1585
Hpy99I CGWCG 5 cut(s) 515, 1854, 1857, 1934, 2022
HpyCH4III ACNGT 4 cut(s) 499, 820, 965, 1816
HpyCH4IV ACGT 5 cut(s) 586, 800, 1477, 1918, 2006
HpyCH4V TGCA 9 cut(s) 247, 455, 519, 763, 776, 833, 1191, 1374, 1508
HpyF10VI GCNNNNNNNGC 9 cut(s) 17, 253, 262, 598, 1175, 1184, 1738, 1745, 1747
HpyF3I CTNAG 9 cut(s) 185, 202, 1038, 1076, 1090, 1146, 1409, 1497, 1523
HpySE526I ACGT 5 cut(s) 586, 800, 1477, 1918, 2006
Hsp92I GRCGYC 1 cut(s) 1664
HspAI GCGC 4 cut(s) 599, 673, 1739, 1748
Ksp22I TGATCA 3 cut(s) 898, 1395, 1609
KspAI GTTAAC 1 cut(s) 882
LguI GCTCTTC 2 cut(s) 28, 99
LmnI GCTCC 8 cut(s) 24, 194, 341, 1348, 1378, 1560, 1885, 1973
Lsp1109I GCAGC 4 cut(s) 53, 75, 760, 1338
LweI GCATC 4 cut(s) 219, 243, 353, 1483
MaeI CTAG 7 cut(s) 581, 612, 848, 1034, 1605, 1619, 1682
MaeII ACGT 5 cut(s) 586, 800, 1477, 1918, 2006
MaeIII GTNAC 3 cut(s) 694, 971, 1822
MbiI CCGCTC 1 cut(s) 1089
MboII GAAGA 9 cut(s) 45, 86, 146, 564, 1075, 1609, 1777, 1903, 1991
MfeI CAATTG 1 cut(s) 951
MflI RGATCY 3 cut(s) 542, 554, 569
MhlI GDGCHC 1 cut(s) 40
MlyI GAGTC 3 cut(s) 161, 788, 1414
MmeI TCCRAC 3 cut(s) 544, 802, 1234
Mph1103I ATGCAT 2 cut(s) 521, 1376
MroXI GAANNNNTTC 1 cut(s) 519
MseI TTAA 9 cut(s) 378, 413, 687, 881, 912, 1107, 1569, 1578, 1637
MslI CAYNNNNRTG 1 cut(s) 1476
MspA1I CMGCKG 2 cut(s) 58, 66
MspI CCGG 1 cut(s) 266
MunI CAATTG 1 cut(s) 951
Mva1269I GAATGC 3 cut(s) 519, 521, 1362
MvnI CGCG 3 cut(s) 1739, 1748, 1849
MwoI GCNNNNNNNGC 9 cut(s) 17, 253, 262, 598, 1175, 1184, 1738, 1745, 1747
NheI GCTAGC 1 cut(s) 1033
NlaIV GGNNCC 4 cut(s) 556, 1428, 1887, 1975
NsbI TGCGCA 1 cut(s) 600
NsiI ATGCAT 2 cut(s) 521, 1376
NspI RCATGY 4 cut(s) 981, 1256, 1374, 1385
PaeI GCATGC 1 cut(s) 1385
PagI TCATGA 1 cut(s) 1024
PaqCI CACCTGC 2 cut(s) 527, 1020
PciI ACATGT 1 cut(s) 1252
PciSI GCTCTTC 2 cut(s) 28, 99
PcsI WCGNNNNNNNCGW 1 cut(s) 1846
PctI GAATGC 3 cut(s) 519, 521, 1362
PdmI GAANNNNTTC 1 cut(s) 519
PfeI GAWTC 9 cut(s) 296, 985, 1042, 1333, 1468, 1643, 1759, 1934, 2022
PflMI CCANNNNNTGG 1 cut(s) 851
PkrI GCNGC 4 cut(s) 65, 68, 775, 1353
PleI GAGTC 3 cut(s) 161, 788, 1413
PpsI GAGTC 3 cut(s) 161, 788, 1413
Ppu21I YACGTR 1 cut(s) 801
PpuMI RGGWCCY 1 cut(s) 1427
PscI ACATGT 1 cut(s) 1252
PshBI ATTAAT 1 cut(s) 912
Psp5II RGGWCCY 1 cut(s) 1427
PspFI CCCAGC 3 cut(s) 58, 256, 652
PspN4I GGNNCC 4 cut(s) 556, 1428, 1887, 1975
PspPI GGNCC 4 cut(s) 115, 268, 429, 1427
PspPPI RGGWCCY 1 cut(s) 1427
PstI CTGCAG 1 cut(s) 778
PsuI RGATCY 3 cut(s) 542, 554, 569
PvuII CAGCTG 2 cut(s) 58, 66
RsaI GTAC 7 cut(s) 51, 1001, 1488, 1624, 1736, 1928, 2016
RsaNI GTAC 7 cut(s) 50, 1000, 1487, 1623, 1735, 1927, 2015
RseI CAYNNNNRTG 1 cut(s) 1476
SapI GCTCTTC 2 cut(s) 28, 99
SaqAI TTAA 9 cut(s) 378, 413, 687, 881, 912, 1107, 1569, 1578, 1637
SatI GCNGC 4 cut(s) 64, 67, 774, 1352
Sau96I GGNCC 4 cut(s) 115, 268, 429, 1427
SchI GAGTC 3 cut(s) 161, 788, 1414
SduI GDGCHC 1 cut(s) 40
SfaNI GCATC 4 cut(s) 219, 243, 353, 1483
SfcI CTRYAG 2 cut(s) 384, 774
SinI GGWCC 3 cut(s) 268, 429, 1427
SmiMI CAYNNNNRTG 1 cut(s) 1476
SnaBI TACGTA 1 cut(s) 801
SphI GCATGC 1 cut(s) 1385
SsiI CCGC 8 cut(s) 432, 440, 1087, 1629, 1847, 1863, 1880, 1968
SspI AATATT 1 cut(s) 1197
SspMI CTAG 7 cut(s) 581, 612, 848, 1034, 1605, 1619, 1682
StyI CCWWGG 1 cut(s) 1430
TaaI ACNGT 4 cut(s) 499, 820, 965, 1816
TaiI ACGT 5 cut(s) 589, 803, 1480, 1921, 2009
TaqI TCGA 9 cut(s) 351, 493, 510, 616, 1803, 1891, 1937, 1979, 2025
TatI WGTACW 1 cut(s) 1486
TfiI GAWTC 9 cut(s) 296, 985, 1042, 1333, 1468, 1643, 1759, 1934, 2022
Tru1I TTAA 9 cut(s) 378, 413, 687, 881, 912, 1107, 1569, 1578, 1637
Tru9I TTAA 9 cut(s) 378, 413, 687, 881, 912, 1107, 1569, 1578, 1637
TscAI CASTG 3 cut(s) 18, 34, 739
TseI GCWGC 4 cut(s) 63, 66, 773, 1351
TspDTI ATGAA 7 cut(s) 17, 118, 459, 1013, 1041, 1460, 1544
TspGWI ACGGA 1 cut(s) 288
TspRI CASTG 3 cut(s) 18, 34, 739
Van91I CCANNNNNTGG 1 cut(s) 851
VpaK11BI GGWCC 3 cut(s) 268, 429, 1427
VspI ATTAAT 1 cut(s) 912
XagI CCTNNNNNAGG 1 cut(s) 1343
XapI RAATTY 2 cut(s) 5, 1139
XbaI TCTAGA 1 cut(s) 580
XceI RCATGY 4 cut(s) 981, 1256, 1374, 1385
XcmI CCANNNNNNNNNTGG 1 cut(s) 818
XmnI GAANNNNTTC 1 cut(s) 519
XspI CTAG 7 cut(s) 581, 612, 848, 1034, 1605, 1619, 1682
Zsp2I ATGCAT 2 cut(s) 521, 1376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.