Rmu_sc0006475.1_g000013

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006475.1
OG0011519
Physical Location & Seq
Reverse (-)
77260 .. 79491
2232 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006475.1_g000013.1.cds

Sequence Viewer

Length: 591 bp
atgcagtggcgcggaatatgcggtgattgggctggtgcagtgcgagctgtgcaggggacgcaggcgtgtgtgcaggtgtgtgggctgctgcaggctggtgtacagcgcggggacgcaggtcgacaggaggctgcagggaggcctgcaggaggctgcagggggggtagctcaggtgagaactttgctcaggagaggatcatctcccaacaaacaaatgtgcgcagcaaagcactgaaatccctgaagagtactactggtcccgatatgtcgacacagtttctggtgttcgctgttcttattatcatcctacagctcttcatctctgtggctcgagccgaagtgcattactacgattttatagttaaggaggtaaacttcactagattgtgtgaatcaaagctcatgttagtagtaaacgagagctatccaggtccagcaatgcgagctcgcagaggggatacaatttatgttaatgtttacaatcaaggattttatggtttcaccattcactggcaagtcccatcacacgacctcccctatctgtttagtgtatcatttacacgatacaacacttcaactttacattcttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 ko01100 map00053 map01100
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

196

Amino Acids

21.68

Weight (kDa)

9.22

Isoelectric Point (pI)

30.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 64
Acc16I TGCGCA 1 cut(s) 221
Acc36I ACCTGC 2 cut(s) 64, 107
AccB7I CCANNNNNTGG 1 cut(s) 510
AccI GTMKAC 2 cut(s) 121, 269
AccII CGCG 2 cut(s) 12, 108
AciI CCGC 3 cut(s) 12, 21, 108
AclWI GGATC 1 cut(s) 203
AcuI CTGAAG 1 cut(s) 263
AfaI GTAC 2 cut(s) 102, 250
AfiI CCNNNNNNNGG 2 cut(s) 149, 510
AgsI TTSAA 1 cut(s) 576
AjnI CCWGG 1 cut(s) 427
AluBI AGCT 6 cut(s) 47, 168, 313, 400, 423, 446
AluI AGCT 6 cut(s) 47, 168, 313, 400, 423, 446
Alw21I GWGCWC 1 cut(s) 448
AlwI GGATC 1 cut(s) 203
AlwNI CAGNNNCTG 1 cut(s) 280
Ama87I CYCGRG 1 cut(s) 330
AoxI GGCC 1 cut(s) 140
ApeKI GCWGC 5 cut(s) 85, 88, 131, 153, 222
AspLEI GCGC 3 cut(s) 12, 108, 222
AspS9I GGNCC 2 cut(s) 257, 431
AsuHPI GGTGA 3 cut(s) 35, 185, 493
AvaI CYCGRG 1 cut(s) 330
AvaII GGWCC 2 cut(s) 257, 431
BanII GRGCYC 1 cut(s) 448
Bbv12I GWGCWC 1 cut(s) 448
BbvI GCAGC 5 cut(s) 72, 75, 118, 140, 234
BccI CCATC 1 cut(s) 529
BciT130I CCWGG 1 cut(s) 429
BciVI GTATCC 1 cut(s) 451
BfaI CTAG 1 cut(s) 381
BfmI CTRYAG 5 cut(s) 89, 132, 144, 154, 308
BfuAI ACCTGC 2 cut(s) 64, 107
BfuI GTATCC 1 cut(s) 451
BisI GCNGC 5 cut(s) 86, 89, 132, 154, 223
BlsI GCNGC 5 cut(s) 87, 90, 133, 155, 224
BmcAI AGTACT 1 cut(s) 250
Bme1390I CCNGG 1 cut(s) 429
Bme18I GGWCC 2 cut(s) 257, 431
BmeT110I CYCGRG 1 cut(s) 330
BmgT120I GGNCC 2 cut(s) 257, 431
BmiI GGNNCC 1 cut(s) 259
BmrFI CCNGG 1 cut(s) 429
BoxI GACNNNNGTC 1 cut(s) 117
Bpu10I CCTNAGC 2 cut(s) 169, 186
BsaXI ACNNNNNCTCC 2 cut(s) 516, 546
Bsc4I CCNNNNNNNGG 2 cut(s) 149, 510
Bse1I ACTGG 2 cut(s) 259, 515
Bse3DI GCAATG 1 cut(s) 444
BseBI CCWGG 1 cut(s) 429
BseGI GGATG 1 cut(s) 303
BseLI CCNNNNNNNGG 2 cut(s) 149, 510
BseMI GCAATG 1 cut(s) 444
BseMII CTCAG 2 cut(s) 183, 200
BseNI ACTGG 2 cut(s) 259, 515
BseXI GCAGC 5 cut(s) 72, 75, 118, 140, 234
BsgI GTGCAG 3 cut(s) 57, 71, 92
Bsh1236I CGCG 2 cut(s) 12, 108
BshFI GGCC 1 cut(s) 142
BsiHKAI GWGCWC 1 cut(s) 448
BsiHKCI CYCGRG 1 cut(s) 330
BslFI GGGAC 4 cut(s) 70, 125, 243, 503
BslI CCNNNNNNNGG 2 cut(s) 149, 510
BsmFI GGGAC 4 cut(s) 70, 125, 243, 503
BsnI GGCC 1 cut(s) 142
BsoBI CYCGRG 1 cut(s) 330
Bsp1286I GDGCHC 1 cut(s) 448
Bsp1407I TGTACA 1 cut(s) 100
Bsp143I GATC 1 cut(s) 195
BspACI CCGC 3 cut(s) 12, 21, 108
BspANI GGCC 1 cut(s) 142
BspCNI CTCAG 2 cut(s) 182, 199
BspFNI CGCG 2 cut(s) 12, 108
BspLI GGNNCC 1 cut(s) 259
BspMAI CTGCAG 4 cut(s) 93, 136, 148, 158
BspMI ACCTGC 2 cut(s) 64, 107
BspPI GGATC 1 cut(s) 203
BspQI GCTCTTC 1 cut(s) 320
BsrDI GCAATG 1 cut(s) 444
BsrGI TGTACA 1 cut(s) 100
BsrI ACTGG 2 cut(s) 259, 515
BssMI GATC 1 cut(s) 195
Bst2UI CCWGG 1 cut(s) 429
Bst4CI ACNGT 1 cut(s) 276
Bst6I CTCTTC 2 cut(s) 239, 320
BstAUI TGTACA 1 cut(s) 100
BstC8I GCNNGC 6 cut(s) 45, 63, 93, 144, 444, 448
BstDEI CTNAG 2 cut(s) 169, 186
BstENI CCTNNNNNAGG 1 cut(s) 147
BstF5I GGATG 1 cut(s) 303
BstFNI CGCG 2 cut(s) 12, 108
BstHHI GCGC 3 cut(s) 12, 108, 222
BstKTI GATC 1 cut(s) 198
BstMBI GATC 1 cut(s) 195
BstMWI GCNNNNNNNGC 5 cut(s) 18, 44, 49, 58, 443
BstNI CCWGG 1 cut(s) 429
BstPAI GACNNNNGTC 1 cut(s) 117
BstSCI CCNGG 1 cut(s) 427
BstSFI CTRYAG 5 cut(s) 89, 132, 144, 154, 308
BstUI CGCG 2 cut(s) 12, 108
BstV1I GCAGC 5 cut(s) 72, 75, 118, 140, 234
BsuI GTATCC 1 cut(s) 451
BsuRI GGCC 1 cut(s) 142
BtsCI GGATG 1 cut(s) 303
BtsI GCAGTG 2 cut(s) 11, 45
BtsIMutI CAGTG 4 cut(s) 11, 45, 230, 508
BveI ACCTGC 2 cut(s) 64, 107
Cac8I GCNNGC 6 cut(s) 45, 63, 93, 144, 444, 448
CaiI CAGNNNCTG 1 cut(s) 280
CfoI GCGC 3 cut(s) 12, 108, 222
Cfr13I GGNCC 2 cut(s) 257, 431
CseI GACGC 2 cut(s) 67, 122
Csp6I GTAC 2 cut(s) 101, 249
CviAII CATG 1 cut(s) 403
CviQI GTAC 2 cut(s) 101, 249
DdeI CTNAG 2 cut(s) 169, 186
DpnI GATC 1 cut(s) 197
DpnII GATC 1 cut(s) 195
Eam1104I CTCTTC 2 cut(s) 239, 320
EarI CTCTTC 2 cut(s) 239, 320
Ecl136II GAGCTC 1 cut(s) 446
Eco147I AGGCCT 1 cut(s) 142
Eco24I GRGCYC 1 cut(s) 448
Eco47I GGWCC 2 cut(s) 257, 431
Eco53kI GAGCTC 1 cut(s) 446
Eco57I CTGAAG 1 cut(s) 263
Eco88I CYCGRG 1 cut(s) 330
EcoICRI GAGCTC 1 cut(s) 446
EcoNI CCTNNNNNAGG 1 cut(s) 147
EcoRII CCWGG 1 cut(s) 427
EcoT38I GRGCYC 1 cut(s) 448
FaeI CATG 1 cut(s) 406
FaiI YATR 6 cut(s) 19, 266, 359, 404, 468, 495
FaqI GGGAC 4 cut(s) 70, 125, 243, 503
FatI CATG 1 cut(s) 402
FauI CCCGC 1 cut(s) 101
FblI GTMKAC 2 cut(s) 121, 269
Fnu4HI GCNGC 5 cut(s) 86, 89, 132, 154, 223
FokI GGATG 1 cut(s) 290
FriOI GRGCYC 1 cut(s) 448
Fsp4HI GCNGC 5 cut(s) 86, 89, 132, 154, 223
FspBI CTAG 1 cut(s) 381
FspI TGCGCA 1 cut(s) 221
GlaI GCGC 3 cut(s) 11, 107, 221
GluI GCNGC 5 cut(s) 86, 89, 132, 154, 223
HaeIII GGCC 1 cut(s) 142
HgaI GACGC 2 cut(s) 67, 122
HhaI GCGC 3 cut(s) 12, 108, 222
Hin1II CATG 1 cut(s) 406
Hin6I GCGC 3 cut(s) 10, 106, 220
HinP1I GCGC 3 cut(s) 10, 106, 220
HincII GTYRAC 2 cut(s) 122, 270
HindII GTYRAC 2 cut(s) 122, 270
HinfI GANTC 1 cut(s) 392
HphI GGTGA 3 cut(s) 35, 185, 493
Hpy166II GTNNAC 6 cut(s) 101, 122, 270, 373, 415, 478
Hpy188III TCNNGA 2 cut(s) 188, 260
Hpy8I GTNNAC 6 cut(s) 101, 122, 270, 373, 415, 478
HpyCH4III ACNGT 1 cut(s) 276
HpyCH4V TGCA 9 cut(s) 4, 38, 52, 73, 91, 134, 146, 156, 343
HpyF10VI GCNNNNNNNGC 5 cut(s) 18, 44, 49, 58, 443
HpyF3I CTNAG 2 cut(s) 169, 186
Hsp92II CATG 1 cut(s) 406
HspAI GCGC 3 cut(s) 10, 106, 220
Kzo9I GATC 1 cut(s) 195
LguI GCTCTTC 1 cut(s) 320
Lsp1109I GCAGC 5 cut(s) 72, 75, 118, 140, 234
MaeI CTAG 1 cut(s) 381
MalI GATC 1 cut(s) 197
MboI GATC 1 cut(s) 195
MboII GAAGA 2 cut(s) 256, 307
MhlI GDGCHC 1 cut(s) 448
MluCI AATT 1 cut(s) 462
MnlI CCTC 7 cut(s) 121, 132, 143, 186, 361, 446, 542
MseI TTAA 3 cut(s) 363, 471, 589
MslI CAYNNNNRTG 1 cut(s) 323
MspR9I CCNGG 1 cut(s) 429
MvaI CCWGG 1 cut(s) 429
MvnI CGCG 2 cut(s) 12, 108
MwoI GCNNNNNNNGC 5 cut(s) 18, 44, 49, 58, 443
NdeII GATC 1 cut(s) 195
NlaIII CATG 1 cut(s) 406
NlaIV GGNNCC 1 cut(s) 259
NsbI TGCGCA 1 cut(s) 221
PaeR7I CTCGAG 1 cut(s) 330
PaqCI CACCTGC 1 cut(s) 64
PceI AGGCCT 1 cut(s) 142
PciSI GCTCTTC 1 cut(s) 320
PfeI GAWTC 1 cut(s) 392
PflMI CCANNNNNTGG 1 cut(s) 510
PkrI GCNGC 5 cut(s) 87, 90, 133, 155, 224
PshAI GACNNNNGTC 1 cut(s) 117
Psp124BI GAGCTC 1 cut(s) 448
Psp6I CCWGG 1 cut(s) 427
PspGI CCWGG 1 cut(s) 427
PspN4I GGNNCC 1 cut(s) 259
PspPI GGNCC 2 cut(s) 257, 431
PspXI VCTCGAGB 1 cut(s) 330
PstI CTGCAG 4 cut(s) 93, 136, 148, 158
PstNI CAGNNNCTG 1 cut(s) 280
RsaI GTAC 2 cut(s) 102, 250
RsaNI GTAC 2 cut(s) 101, 249
RseI CAYNNNNRTG 1 cut(s) 323
SacI GAGCTC 1 cut(s) 448
SalI GTCGAC 2 cut(s) 120, 268
SapI GCTCTTC 1 cut(s) 320
SaqAI TTAA 3 cut(s) 363, 471, 589
SatI GCNGC 5 cut(s) 86, 89, 132, 154, 223
Sau3AI GATC 1 cut(s) 195
Sau96I GGNCC 2 cut(s) 257, 431
SbfI CCTGCAGG 1 cut(s) 148
ScaI AGTACT 1 cut(s) 250
ScrFI CCNGG 1 cut(s) 429
SdaI CCTGCAGG 1 cut(s) 148
SduI GDGCHC 1 cut(s) 448
SfcI CTRYAG 5 cut(s) 89, 132, 144, 154, 308
Sfr274I CTCGAG 1 cut(s) 330
SinI GGWCC 2 cut(s) 257, 431
SlaI CTCGAG 1 cut(s) 330
SmiMI CAYNNNNRTG 1 cut(s) 323
SmlI CTYRAG 1 cut(s) 330
SmoI CTYRAG 1 cut(s) 330
Sse8387I CCTGCAGG 1 cut(s) 148
Sse9I AATT 1 cut(s) 462
SseBI AGGCCT 1 cut(s) 142
SsiI CCGC 3 cut(s) 12, 21, 108
SspMI CTAG 1 cut(s) 381
SstI GAGCTC 1 cut(s) 448
StuI AGGCCT 1 cut(s) 142
StyD4I CCNGG 1 cut(s) 427
TaaI ACNGT 1 cut(s) 276
TaqI TCGA 3 cut(s) 121, 269, 331
TasI AATT 1 cut(s) 462
TatI WGTACW 2 cut(s) 100, 248
TfiI GAWTC 1 cut(s) 392
Tru1I TTAA 3 cut(s) 363, 471, 589
Tru9I TTAA 3 cut(s) 363, 471, 589
TscAI CASTG 3 cut(s) 45, 237, 515
TseI GCWGC 5 cut(s) 85, 88, 131, 153, 222
TspDTI ATGAA 1 cut(s) 307
TspRI CASTG 3 cut(s) 45, 237, 515
Van91I CCANNNNNTGG 1 cut(s) 510
VpaK11BI GGWCC 2 cut(s) 257, 431
XagI CCTNNNNNAGG 1 cut(s) 147
XhoI CTCGAG 1 cut(s) 330
XmiI GTMKAC 2 cut(s) 121, 269
XspI CTAG 1 cut(s) 381
ZrmI AGTACT 1 cut(s) 250
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.