Rh2BG520700

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0011519
Physical Location & Seq
Reverse (-)
73033286 .. 73035065
1780 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG520700.1

Sequence Viewer

Length: 582 bp
ATGAAATCCATGAAGAGTACTACTAGTCCAGTACTGATGTCGACACAGTTTCTGGTGTTCGCTGCTCTTATTATCATCCTACCGCTCTTCATCTCTCTGGCTCGAGCAGAAGTTCATTACTACGATTTTATAGTTAAGGAGGTAAACTTCACTAGATTGTGTGAATCAAAGCTCATGTTAGTAGTAAACGAGAGCTATCCAGGTCCAGCAATACGAGCTCGCAAAGGGGATACAATTTATGTTAATGTTTACAATCAAGGATTTTATGGTTTCACCATTCACTGGCAAGTCCCATCACACGACCTCCCCTATCTGTTTAGTCAAATTCCCAGTTCCTTATCCCAAACAGATGCATTCAACTTCTACGGAAATATTAGAAGCTTAGCCACCCCAGAGTATCCTATAAATGTCCCAAAAGAGAGCGACATAACTACCAAAATGTACATAACAGCTTCCGCCAACGCTCTTTATTGTACTCCAGCTTTAGGTCCCTCATGCGTAAACATATCTTTTGCTGCAAGCGTCAATAATATCAGTTGGGTCAATCCGAGAGAATCTATCTTGCAAGCCTACTACAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 ko01100 map00053 map01100
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

193

Amino Acids

21.75

Weight (kDa)

8.38

Isoelectric Point (pI)

51.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 45 - 96 2.4e-10 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 282
AccBSI CCGCTC 1 cut(s) 85
AccI GTMKAC 1 cut(s) 41
AciI CCGC 2 cut(s) 83, 456
AcsI RAATTY 1 cut(s) 324
AfaI GTAC 4 cut(s) 19, 33, 443, 475
AfiI CCNNNNNNNGG 3 cut(s) 282, 485, 576
AgsI TTSAA 1 cut(s) 358
AhlI ACTAGT 1 cut(s) 23
AjnI CCWGG 1 cut(s) 199
AluBI AGCT 6 cut(s) 172, 195, 218, 381, 452, 482
AluI AGCT 6 cut(s) 172, 195, 218, 381, 452, 482
Alw21I GWGCWC 1 cut(s) 220
AlwNI CAGNNNCTG 1 cut(s) 52
Ama87I CYCGRG 1 cut(s) 102
ApeKI GCWGC 2 cut(s) 62, 515
ApoI RAATTY 1 cut(s) 324
AspS9I GGNCC 2 cut(s) 203, 488
AsuHPI GGTGA 1 cut(s) 265
AvaI CYCGRG 1 cut(s) 102
AvaII GGWCC 2 cut(s) 203, 488
BanII GRGCYC 1 cut(s) 220
Bbv12I GWGCWC 1 cut(s) 220
BbvI GCAGC 2 cut(s) 49, 502
BccI CCATC 1 cut(s) 301
BciT130I CCWGG 1 cut(s) 201
BciVI GTATCC 2 cut(s) 223, 408
BcuI ACTAGT 1 cut(s) 23
BfaI CTAG 2 cut(s) 24, 153
BfmI CTRYAG 1 cut(s) 574
BfuI GTATCC 2 cut(s) 223, 408
BisI GCNGC 2 cut(s) 63, 516
BlpI GCTNAGC 1 cut(s) 382
BlsI GCNGC 2 cut(s) 64, 517
BmcAI AGTACT 2 cut(s) 19, 33
Bme1390I CCNGG 1 cut(s) 201
Bme18I GGWCC 2 cut(s) 203, 488
BmeT110I CYCGRG 1 cut(s) 102
BmgT120I GGNCC 2 cut(s) 203, 488
BmiI GGNNCC 1 cut(s) 490
BmrFI CCNGG 1 cut(s) 201
BmrI ACTGGG 1 cut(s) 324
BmsI GCATC 1 cut(s) 340
BmuI ACTGGG 1 cut(s) 324
BpmI CTGGAG 1 cut(s) 462
Bpu1102I GCTNAGC 1 cut(s) 382
BsaXI ACNNNNNCTCC 2 cut(s) 288, 318
Bsc4I CCNNNNNNNGG 3 cut(s) 282, 485, 576
Bse1I ACTGG 3 cut(s) 29, 287, 330
BseBI CCWGG 1 cut(s) 201
BseGI GGATG 1 cut(s) 75
BseLI CCNNNNNNNGG 3 cut(s) 282, 485, 576
BseNI ACTGG 3 cut(s) 29, 287, 330
BseXI GCAGC 2 cut(s) 49, 502
BsiHKAI GWGCWC 1 cut(s) 220
BsiHKCI CYCGRG 1 cut(s) 102
BslFI GGGAC 3 cut(s) 275, 395, 474
BslI CCNNNNNNNGG 3 cut(s) 282, 485, 576
BsmFI GGGAC 3 cut(s) 275, 395, 474
BsmI GAATGC 1 cut(s) 353
BsoBI CYCGRG 1 cut(s) 102
Bsp1286I GDGCHC 1 cut(s) 220
Bsp1407I TGTACA 1 cut(s) 441
Bsp1720I GCTNAGC 1 cut(s) 382
BspACI CCGC 2 cut(s) 83, 456
BspLI GGNNCC 1 cut(s) 490
BspQI GCTCTTC 1 cut(s) 92
BsrBI CCGCTC 1 cut(s) 85
BsrGI TGTACA 1 cut(s) 441
BsrI ACTGG 3 cut(s) 29, 287, 330
Bst2UI CCWGG 1 cut(s) 201
Bst4CI ACNGT 1 cut(s) 48
Bst6I CTCTTC 2 cut(s) 8, 92
BstAUI TGTACA 1 cut(s) 441
BstC8I GCNNGC 3 cut(s) 220, 520, 567
BstDEI CTNAG 1 cut(s) 382
BstENI CCTNNNNNAGG 1 cut(s) 574
BstF5I GGATG 1 cut(s) 75
BstMWI GCNNNNNNNGC 1 cut(s) 215
BstNI CCWGG 1 cut(s) 201
BstSCI CCNGG 1 cut(s) 199
BstSFI CTRYAG 1 cut(s) 574
BstV1I GCAGC 2 cut(s) 49, 502
BsuI GTATCC 2 cut(s) 223, 408
BtsCI GGATG 1 cut(s) 75
BtsIMutI CAGTG 1 cut(s) 280
Cac8I GCNNGC 3 cut(s) 220, 520, 567
CaiI CAGNNNCTG 1 cut(s) 52
Cfr13I GGNCC 2 cut(s) 203, 488
CseI GACGC 1 cut(s) 511
Csp6I GTAC 4 cut(s) 18, 32, 442, 474
CviAII CATG 3 cut(s) 10, 175, 495
CviJI RGCY 9 cut(s) 101, 172, 195, 218, 381, 386, 452, 482, 569
CviKI_1 RGCY 9 cut(s) 101, 172, 195, 218, 381, 386, 452, 482, 569
CviQI GTAC 4 cut(s) 18, 32, 442, 474
DdeI CTNAG 1 cut(s) 382
Eam1104I CTCTTC 2 cut(s) 8, 92
EarI CTCTTC 2 cut(s) 8, 92
EciI GGCGGA 1 cut(s) 445
Ecl136II GAGCTC 1 cut(s) 218
Eco24I GRGCYC 1 cut(s) 220
Eco47I GGWCC 2 cut(s) 203, 488
Eco53kI GAGCTC 1 cut(s) 218
Eco88I CYCGRG 1 cut(s) 102
EcoICRI GAGCTC 1 cut(s) 218
EcoNI CCTNNNNNAGG 1 cut(s) 574
EcoO109I RGGNCCY 1 cut(s) 488
EcoRII CCWGG 1 cut(s) 199
EcoT22I ATGCAT 1 cut(s) 355
EcoT38I GRGCYC 1 cut(s) 220
FaeI CATG 3 cut(s) 13, 178, 498
FaqI GGGAC 3 cut(s) 275, 395, 474
FatI CATG 3 cut(s) 9, 174, 494
FblI GTMKAC 1 cut(s) 41
Fnu4HI GCNGC 2 cut(s) 63, 516
FokI GGATG 1 cut(s) 62
FriOI GRGCYC 1 cut(s) 220
Fsp4HI GCNGC 2 cut(s) 63, 516
FspBI CTAG 2 cut(s) 24, 153
GluI GCNGC 2 cut(s) 63, 516
GsuI CTGGAG 1 cut(s) 462
HgaI GACGC 1 cut(s) 511
Hin1II CATG 3 cut(s) 13, 178, 498
HincII GTYRAC 1 cut(s) 42
HindII GTYRAC 1 cut(s) 42
HindIII AAGCTT 1 cut(s) 379
HinfI GANTC 2 cut(s) 164, 554
HphI GGTGA 1 cut(s) 265
Hpy166II GTNNAC 5 cut(s) 42, 145, 187, 250, 502
Hpy188I TCNGA 1 cut(s) 549
Hpy8I GTNNAC 5 cut(s) 42, 145, 187, 250, 502
HpyCH4III ACNGT 1 cut(s) 48
HpyCH4V TGCA 3 cut(s) 353, 518, 565
HpyF10VI GCNNNNNNNGC 1 cut(s) 215
HpyF3I CTNAG 1 cut(s) 382
Hsp92II CATG 3 cut(s) 13, 178, 498
LguI GCTCTTC 1 cut(s) 92
Lsp1109I GCAGC 2 cut(s) 49, 502
LweI GCATC 1 cut(s) 340
MaeI CTAG 2 cut(s) 24, 153
MbiI CCGCTC 1 cut(s) 85
MboII GAAGA 2 cut(s) 25, 79
MhlI GDGCHC 1 cut(s) 220
MluCI AATT 2 cut(s) 234, 324
MnlI CCTC 3 cut(s) 133, 314, 502
Mph1103I ATGCAT 1 cut(s) 355
MseI TTAA 2 cut(s) 135, 243
MspR9I CCNGG 1 cut(s) 201
Mva1269I GAATGC 1 cut(s) 353
MvaI CCWGG 1 cut(s) 201
MwoI GCNNNNNNNGC 1 cut(s) 215
NlaIII CATG 3 cut(s) 13, 178, 498
NlaIV GGNNCC 1 cut(s) 490
NsiI ATGCAT 1 cut(s) 355
PaeR7I CTCGAG 1 cut(s) 102
PciSI GCTCTTC 1 cut(s) 92
PctI GAATGC 1 cut(s) 353
PfeI GAWTC 2 cut(s) 164, 554
PflMI CCANNNNNTGG 1 cut(s) 282
PkrI GCNGC 2 cut(s) 64, 517
PpuMI RGGWCCY 1 cut(s) 488
Psp124BI GAGCTC 1 cut(s) 220
Psp5II RGGWCCY 1 cut(s) 488
Psp6I CCWGG 1 cut(s) 199
PspGI CCWGG 1 cut(s) 199
PspN4I GGNNCC 1 cut(s) 490
PspPI GGNCC 2 cut(s) 203, 488
PspPPI RGGWCCY 1 cut(s) 488
PspXI VCTCGAGB 1 cut(s) 102
PstNI CAGNNNCTG 1 cut(s) 52
RsaI GTAC 4 cut(s) 19, 33, 443, 475
RsaNI GTAC 4 cut(s) 18, 32, 442, 474
SacI GAGCTC 1 cut(s) 220
SalI GTCGAC 1 cut(s) 40
SapI GCTCTTC 1 cut(s) 92
SaqAI TTAA 2 cut(s) 135, 243
SatI GCNGC 2 cut(s) 63, 516
Sau96I GGNCC 2 cut(s) 203, 488
ScaI AGTACT 2 cut(s) 19, 33
ScrFI CCNGG 1 cut(s) 201
SduI GDGCHC 1 cut(s) 220
SfaNI GCATC 1 cut(s) 340
SfcI CTRYAG 1 cut(s) 574
Sfr274I CTCGAG 1 cut(s) 102
SinI GGWCC 2 cut(s) 203, 488
SlaI CTCGAG 1 cut(s) 102
SmlI CTYRAG 1 cut(s) 102
SmoI CTYRAG 1 cut(s) 102
SpeI ACTAGT 1 cut(s) 23
Sse9I AATT 2 cut(s) 234, 324
SsiI CCGC 2 cut(s) 83, 456
SspI AATATT 1 cut(s) 373
SspMI CTAG 2 cut(s) 24, 153
SstI GAGCTC 1 cut(s) 220
StyD4I CCNGG 1 cut(s) 199
TaaI ACNGT 1 cut(s) 48
TaqI TCGA 2 cut(s) 41, 103
TasI AATT 2 cut(s) 234, 324
TatI WGTACW 4 cut(s) 17, 31, 441, 473
TfiI GAWTC 2 cut(s) 164, 554
Tru1I TTAA 2 cut(s) 135, 243
Tru9I TTAA 2 cut(s) 135, 243
TscAI CASTG 1 cut(s) 287
TseI GCWGC 2 cut(s) 62, 515
TspDTI ATGAA 4 cut(s) 17, 26, 79, 104
TspGWI ACGGA 1 cut(s) 381
TspRI CASTG 1 cut(s) 287
Van91I CCANNNNNTGG 1 cut(s) 282
VpaK11BI GGWCC 2 cut(s) 203, 488
XagI CCTNNNNNAGG 1 cut(s) 574
XapI RAATTY 1 cut(s) 324
XhoI CTCGAG 1 cut(s) 102
XmiI GTMKAC 1 cut(s) 41
XspI CTAG 2 cut(s) 24, 153
ZrmI AGTACT 2 cut(s) 19, 33
Zsp2I ATGCAT 1 cut(s) 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.