Rroxscaffold_7G00195640

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0011519
Physical Location & Seq
Reverse (-)
38155594 .. 38156470
877 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00195640.1

Sequence Viewer

Length: 438 bp
ATGCATCTAGATATGAGGGCATGTCGGTATGAGGGTTATACAAATCAAGTAGGACAGAAACGAAAGCAAGAAGCTGAATCCTCCGGTGCGAGAGCAAACAAAACTGGAGGAAATTCAGATCATCCAATTTTAGAAATGGGTTGCCGCATACTCGTCCTCCTCCTGGGAACTTTCTTCCTTGATGGGCTTCTCCTCTCCATGGCTGCTAACGTCCACCACTACAGCTTTATTCTGAAGGAGACCAATTTCACGAGACTATGTACCACAAAGCCAATGCTGACTGTAAATGGGCAGTTCCCAGGTCCAACCATCTCTGTTCGCAAAGGAGATAGAGCTTTTGTTAATGTCCACAACAATGGCAAATATGGTGTCACCATTCACTGGTCTCTCTCTCTCTCTCTCAGTGTACAATCAGATGCAGATCCAACTGCTCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

145

Amino Acids

16.02

Weight (kDa)

9.12

Isoelectric Point (pI)

26.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 79 - 129 3e-13 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 381
AciI CCGC 1 cut(s) 145
AclWI GGATC 1 cut(s) 416
AcsI RAATTY 1 cut(s) 112
AcuI CTGAAG 1 cut(s) 254
AfaI GTAC 2 cut(s) 262, 408
AfiI CCNNNNNNNGG 3 cut(s) 163, 199, 381
AjnI CCWGG 2 cut(s) 162, 298
AluBI AGCT 3 cut(s) 74, 225, 335
AluI AGCT 3 cut(s) 74, 225, 335
Alw26I GTCTC 3 cut(s) 233, 247, 390
AlwI GGATC 1 cut(s) 416
ApeKI GCWGC 1 cut(s) 203
ApoI RAATTY 1 cut(s) 112
ArsI GACNNNNNNTTYG 2 cut(s) 354, 386
AspS9I GGNCC 1 cut(s) 302
AsuHPI GGTGA 1 cut(s) 364
AvaII GGWCC 1 cut(s) 302
BauI CACGAG 1 cut(s) 250
BbvI GCAGC 1 cut(s) 190
BccI CCATC 2 cut(s) 176, 317
BciT130I CCWGG 2 cut(s) 164, 300
BcoDI GTCTC 3 cut(s) 233, 247, 390
BfaI CTAG 1 cut(s) 8
BfmI CTRYAG 1 cut(s) 220
BisI GCNGC 2 cut(s) 145, 204
BlsI GCNGC 2 cut(s) 146, 205
Bme1390I CCNGG 2 cut(s) 164, 300
Bme18I GGWCC 1 cut(s) 302
BmgT120I GGNCC 1 cut(s) 302
BmrFI CCNGG 2 cut(s) 164, 300
BmsI GCATC 2 cut(s) 13, 406
BpmI CTGGAG 1 cut(s) 126
BsaBI GATNNNNATC 1 cut(s) 420
BsaI GGTCTC 2 cut(s) 233, 390
BsaJI CCNNGG 3 cut(s) 163, 198, 298
BsaWI WCCGGW 1 cut(s) 83
BsaXI ACNNNNNCTCC 2 cut(s) 141, 171
Bsc4I CCNNNNNNNGG 3 cut(s) 163, 199, 381
Bse1I ACTGG 2 cut(s) 109, 386
Bse8I GATNNNNATC 1 cut(s) 420
BseBI CCWGG 2 cut(s) 164, 300
BseDI CCNNGG 3 cut(s) 163, 198, 298
BseGI GGATG 1 cut(s) 121
BseJI GATNNNNATC 1 cut(s) 420
BseLI CCNNNNNNNGG 3 cut(s) 163, 199, 381
BseMII CTCAG 1 cut(s) 415
BseNI ACTGG 2 cut(s) 109, 386
BseRI GAGGAG 2 cut(s) 149, 182
BseXI GCAGC 1 cut(s) 190
BsiSI CCGG 1 cut(s) 84
BslI CCNNNNNNNGG 3 cut(s) 163, 199, 381
BsmAI GTCTC 3 cut(s) 233, 247, 390
Bso31I GGTCTC 2 cut(s) 233, 390
Bsp1407I TGTACA 1 cut(s) 406
Bsp143I GATC 2 cut(s) 118, 421
Bsp19I CCATGG 1 cut(s) 198
BspACI CCGC 1 cut(s) 145
BspCNI CTCAG 1 cut(s) 414
BspPI GGATC 1 cut(s) 416
BspTNI GGTCTC 2 cut(s) 233, 390
BsrGI TGTACA 1 cut(s) 406
BsrI ACTGG 2 cut(s) 109, 386
BssECI CCNNGG 3 cut(s) 163, 198, 298
BssMI GATC 2 cut(s) 118, 421
BssSI CACGAG 1 cut(s) 250
BssT1I CCWWGG 1 cut(s) 198
Bst2BI CACGAG 1 cut(s) 250
Bst2UI CCWGG 2 cut(s) 164, 300
Bst4CI ACNGT 1 cut(s) 283
BstAUI TGTACA 1 cut(s) 406
BstDEI CTNAG 1 cut(s) 401
BstDSI CCRYGG 1 cut(s) 198
BstF5I GGATG 1 cut(s) 121
BstKTI GATC 2 cut(s) 121, 424
BstMAI GTCTC 3 cut(s) 233, 247, 390
BstMBI GATC 2 cut(s) 118, 421
BstNI CCWGG 2 cut(s) 164, 300
BstNSI RCATGY 1 cut(s) 24
BstSCI CCNGG 2 cut(s) 162, 298
BstSFI CTRYAG 1 cut(s) 220
BstV1I GCAGC 1 cut(s) 190
BstX2I RGATCY 1 cut(s) 421
BstXI CCANNNNNNTGG 1 cut(s) 356
BstYI RGATCY 1 cut(s) 421
BtgI CCRYGG 1 cut(s) 198
BtsCI GGATG 1 cut(s) 121
BtsIMutI CAGTG 2 cut(s) 379, 409
Cfr13I GGNCC 1 cut(s) 302
Csp6I GTAC 2 cut(s) 261, 407
CviAII CATG 2 cut(s) 21, 199
CviJI RGCY 6 cut(s) 74, 187, 203, 225, 271, 335
CviKI_1 RGCY 6 cut(s) 74, 187, 203, 225, 271, 335
CviQI GTAC 2 cut(s) 261, 407
DdeI CTNAG 1 cut(s) 401
DpnI GATC 2 cut(s) 120, 423
DpnII GATC 2 cut(s) 118, 421
Eco130I CCWWGG 1 cut(s) 198
Eco31I GGTCTC 2 cut(s) 233, 390
Eco47I GGWCC 1 cut(s) 302
Eco57I CTGAAG 1 cut(s) 254
EcoRII CCWGG 2 cut(s) 162, 298
EcoT14I CCWWGG 1 cut(s) 198
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 198
FaeI CATG 2 cut(s) 24, 202
FaiI YATR 8 cut(s) 14, 22, 30, 39, 149, 200, 259, 366
FatI CATG 2 cut(s) 20, 198
Fnu4HI GCNGC 2 cut(s) 145, 204
FokI GGATG 1 cut(s) 108
Fsp4HI GCNGC 2 cut(s) 145, 204
FspBI CTAG 1 cut(s) 8
GluI GCNGC 2 cut(s) 145, 204
GsuI CTGGAG 1 cut(s) 126
HapII CCGG 1 cut(s) 84
Hin1II CATG 2 cut(s) 24, 202
HinfI GANTC 1 cut(s) 77
HpaII CCGG 1 cut(s) 84
HphI GGTGA 1 cut(s) 364
Hpy166II GTNNAC 3 cut(s) 214, 349, 407
Hpy188I TCNGA 3 cut(s) 118, 234, 415
Hpy188III TCNNGA 2 cut(s) 8, 250
Hpy8I GTNNAC 3 cut(s) 214, 349, 407
HpyAV CCTTC 1 cut(s) 229
HpyCH4III ACNGT 1 cut(s) 283
HpyCH4IV ACGT 1 cut(s) 210
HpyCH4V TGCA 2 cut(s) 4, 419
HpyF3I CTNAG 1 cut(s) 401
HpySE526I ACGT 1 cut(s) 210
Hsp92II CATG 2 cut(s) 24, 202
Kzo9I GATC 2 cut(s) 118, 421
LpnPI CCDG 7 cut(s) 90, 97, 149, 176, 285, 312, 367
Lsp1109I GCAGC 1 cut(s) 190
LweI GCATC 2 cut(s) 13, 406
MaeI CTAG 1 cut(s) 8
MaeII ACGT 1 cut(s) 210
MaeIII GTNAC 1 cut(s) 370
MalI GATC 2 cut(s) 120, 423
MboI GATC 2 cut(s) 118, 421
MboII GAAGA 1 cut(s) 166
MflI RGATCY 1 cut(s) 421
MluCI AATT 3 cut(s) 112, 126, 244
MmeI TCCRAC 1 cut(s) 329
MnlI CCTC 7 cut(s) 9, 25, 91, 101, 167, 170, 203
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 1 cut(s) 342
MslI CAYNNNNRTG 1 cut(s) 354
MspI CCGG 1 cut(s) 84
MspR9I CCNGG 2 cut(s) 164, 300
MvaI CCWGG 2 cut(s) 164, 300
NcoI CCATGG 1 cut(s) 198
NdeII GATC 2 cut(s) 118, 421
NlaIII CATG 2 cut(s) 24, 202
NmuCI GTSAC 1 cut(s) 370
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 24
PfeI GAWTC 1 cut(s) 77
PflMI CCANNNNNTGG 1 cut(s) 381
PkrI GCNGC 2 cut(s) 146, 205
Psp6I CCWGG 2 cut(s) 162, 298
PspGI CCWGG 2 cut(s) 162, 298
PspPI GGNCC 1 cut(s) 302
PsuI RGATCY 1 cut(s) 421
RsaI GTAC 2 cut(s) 262, 408
RsaNI GTAC 2 cut(s) 261, 407
RseI CAYNNNNRTG 1 cut(s) 354
SaqAI TTAA 1 cut(s) 342
SatI GCNGC 2 cut(s) 145, 204
Sau3AI GATC 2 cut(s) 118, 421
Sau96I GGNCC 1 cut(s) 302
ScrFI CCNGG 2 cut(s) 164, 300
SetI ASST 5 cut(s) 76, 213, 227, 304, 337
SfaNI GCATC 2 cut(s) 13, 406
SfcI CTRYAG 1 cut(s) 220
SinI GGWCC 1 cut(s) 302
SmiMI CAYNNNNRTG 1 cut(s) 354
Sse9I AATT 3 cut(s) 112, 126, 244
SsiI CCGC 1 cut(s) 145
SspMI CTAG 1 cut(s) 8
StyD4I CCNGG 2 cut(s) 162, 298
StyI CCWWGG 1 cut(s) 198
TaaI ACNGT 1 cut(s) 283
TaiI ACGT 1 cut(s) 213
TasI AATT 3 cut(s) 112, 126, 244
TatI WGTACW 1 cut(s) 406
TauI GCSGC 1 cut(s) 147
TfiI GAWTC 1 cut(s) 77
Tru1I TTAA 1 cut(s) 342
Tru9I TTAA 1 cut(s) 342
TscAI CASTG 2 cut(s) 386, 409
TseFI GTSAC 1 cut(s) 370
TseI GCWGC 1 cut(s) 203
Tsp45I GTSAC 1 cut(s) 370
TspRI CASTG 2 cut(s) 386, 409
Van91I CCANNNNNTGG 1 cut(s) 381
VpaK11BI GGWCC 1 cut(s) 302
XapI RAATTY 1 cut(s) 112
XbaI TCTAGA 1 cut(s) 7
XceI RCATGY 1 cut(s) 24
XspI CTAG 1 cut(s) 8
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.