Rh2AG509500

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0011519
Physical Location & Seq
Reverse (-)
73441774 .. 73442417
644 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG509500.1

Sequence Viewer

Length: 462 bp
ATGAAAGCAACAGAGCTACGGAAAGCAATAAAGTTAGTGAGTGTCCATGCAAATCCTAACATTTCAAGTTCTTCACAAACAAAAGCGCTCAGCAGAATCATGAAATCCATGAAGAGTACTACTAGTCCAGTACTGATGTCGACACAGTTTCTGGTGTTCGCTGCTCTTATTATCATCCTACCGCTCTTCATCTCTCTGGCTCGAGCAGAAGTTCATTACTACGATTTTATAGTTAAGGAGGTAAACTTCACTAGATTGTGTGAATCAAAGCTCATGTTAGTAGTAAACGAGAGCTATCCAGGTCCAGCAATACGAGCTCGCAAAGGGGATACAATTTATGTTAATGTTTACAATCAAGGATTTTATGGTTTCACCATTCACTGGCAAGTCCCATCACACGACCTCCCCTATCTGTTTAGTGTATCCTTTACACGATACAACACTTCTACTTTACATTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 ko01100 map00053 map01100
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

153

Amino Acids

17.36

Weight (kDa)

9.75

Isoelectric Point (pI)

37.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 78 - 129 1.5e-10 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 381
AccBSI CCGCTC 1 cut(s) 184
AccI GTMKAC 1 cut(s) 140
AciI CCGC 1 cut(s) 182
AfaI GTAC 2 cut(s) 118, 132
AfeI AGCGCT 1 cut(s) 87
AfiI CCNNNNNNNGG 1 cut(s) 381
AgsI TTSAA 1 cut(s) 66
AhlI ACTAGT 1 cut(s) 122
AjnI CCWGG 1 cut(s) 298
AluBI AGCT 4 cut(s) 16, 271, 294, 317
AluI AGCT 4 cut(s) 16, 271, 294, 317
Alw21I GWGCWC 1 cut(s) 319
AlwNI CAGNNNCTG 1 cut(s) 151
Ama87I CYCGRG 1 cut(s) 201
Aor51HI AGCGCT 1 cut(s) 87
ApeKI GCWGC 1 cut(s) 161
AspLEI GCGC 1 cut(s) 88
AspS9I GGNCC 1 cut(s) 302
AsuHPI GGTGA 1 cut(s) 364
AvaI CYCGRG 1 cut(s) 201
AvaII GGWCC 1 cut(s) 302
BanII GRGCYC 1 cut(s) 319
Bbv12I GWGCWC 1 cut(s) 319
BbvI GCAGC 1 cut(s) 148
BccI CCATC 1 cut(s) 400
BciT130I CCWGG 1 cut(s) 300
BciVI GTATCC 2 cut(s) 322, 433
BcuI ACTAGT 1 cut(s) 122
BfaI CTAG 2 cut(s) 123, 252
BfoI RGCGCY 1 cut(s) 89
BfuI GTATCC 2 cut(s) 322, 433
BisI GCNGC 1 cut(s) 162
BlpI GCTNAGC 1 cut(s) 89
BlsI GCNGC 1 cut(s) 163
BmcAI AGTACT 2 cut(s) 118, 132
Bme1390I CCNGG 1 cut(s) 300
Bme18I GGWCC 1 cut(s) 302
BmeT110I CYCGRG 1 cut(s) 201
BmgT120I GGNCC 1 cut(s) 302
BmrFI CCNGG 1 cut(s) 300
Bpu1102I GCTNAGC 1 cut(s) 89
BsaXI ACNNNNNCTCC 2 cut(s) 387, 417
Bsc4I CCNNNNNNNGG 1 cut(s) 381
Bse1I ACTGG 2 cut(s) 128, 386
BseBI CCWGG 1 cut(s) 300
BseGI GGATG 1 cut(s) 174
BseLI CCNNNNNNNGG 1 cut(s) 381
BseMII CTCAG 1 cut(s) 103
BseNI ACTGG 2 cut(s) 128, 386
BseXI GCAGC 1 cut(s) 148
BsiHKAI GWGCWC 1 cut(s) 319
BsiHKCI CYCGRG 1 cut(s) 201
BslFI GGGAC 1 cut(s) 374
BslI CCNNNNNNNGG 1 cut(s) 381
BsmFI GGGAC 1 cut(s) 374
BsoBI CYCGRG 1 cut(s) 201
Bsp1286I GDGCHC 1 cut(s) 319
Bsp1720I GCTNAGC 1 cut(s) 89
BspACI CCGC 1 cut(s) 182
BspCNI CTCAG 1 cut(s) 102
BspHI TCATGA 1 cut(s) 99
BspQI GCTCTTC 1 cut(s) 191
BsrBI CCGCTC 1 cut(s) 184
BsrI ACTGG 2 cut(s) 128, 386
Bst2UI CCWGG 1 cut(s) 300
Bst4CI ACNGT 1 cut(s) 147
Bst6I CTCTTC 2 cut(s) 107, 191
BstC8I GCNNGC 1 cut(s) 319
BstDEI CTNAG 1 cut(s) 89
BstF5I GGATG 1 cut(s) 174
BstH2I RGCGCY 1 cut(s) 89
BstHHI GCGC 1 cut(s) 88
BstMWI GCNNNNNNNGC 1 cut(s) 314
BstNI CCWGG 1 cut(s) 300
BstSCI CCNGG 1 cut(s) 298
BstV1I GCAGC 1 cut(s) 148
BsuI GTATCC 2 cut(s) 322, 433
BtsCI GGATG 1 cut(s) 174
BtsIMutI CAGTG 1 cut(s) 379
Cac8I GCNNGC 1 cut(s) 319
CaiI CAGNNNCTG 1 cut(s) 151
CciI TCATGA 1 cut(s) 99
CfoI GCGC 1 cut(s) 88
Cfr13I GGNCC 1 cut(s) 302
Csp6I GTAC 2 cut(s) 117, 131
CviAII CATG 4 cut(s) 47, 100, 109, 274
CviJI RGCY 5 cut(s) 16, 200, 271, 294, 317
CviKI_1 RGCY 5 cut(s) 16, 200, 271, 294, 317
CviQI GTAC 2 cut(s) 117, 131
DdeI CTNAG 1 cut(s) 89
Eam1104I CTCTTC 2 cut(s) 107, 191
EarI CTCTTC 2 cut(s) 107, 191
Ecl136II GAGCTC 1 cut(s) 317
Eco24I GRGCYC 1 cut(s) 319
Eco47I GGWCC 1 cut(s) 302
Eco47III AGCGCT 1 cut(s) 87
Eco53kI GAGCTC 1 cut(s) 317
Eco88I CYCGRG 1 cut(s) 201
EcoICRI GAGCTC 1 cut(s) 317
EcoRII CCWGG 1 cut(s) 298
EcoT38I GRGCYC 1 cut(s) 319
FaeI CATG 4 cut(s) 50, 103, 112, 277
FaiI YATR 7 cut(s) 48, 101, 110, 230, 275, 339, 366
FaqI GGGAC 1 cut(s) 374
FatI CATG 4 cut(s) 46, 99, 108, 273
FblI GTMKAC 1 cut(s) 140
Fnu4HI GCNGC 1 cut(s) 162
FokI GGATG 1 cut(s) 161
FriOI GRGCYC 1 cut(s) 319
Fsp4HI GCNGC 1 cut(s) 162
FspBI CTAG 2 cut(s) 123, 252
GlaI GCGC 1 cut(s) 87
GluI GCNGC 1 cut(s) 162
HaeII RGCGCY 1 cut(s) 89
HhaI GCGC 1 cut(s) 88
Hin1II CATG 4 cut(s) 50, 103, 112, 277
Hin6I GCGC 1 cut(s) 86
HinP1I GCGC 1 cut(s) 86
HincII GTYRAC 1 cut(s) 141
HindII GTYRAC 1 cut(s) 141
HinfI GANTC 2 cut(s) 96, 263
HphI GGTGA 1 cut(s) 364
Hpy166II GTNNAC 4 cut(s) 141, 244, 286, 349
Hpy188III TCNNGA 1 cut(s) 100
Hpy8I GTNNAC 4 cut(s) 141, 244, 286, 349
HpyCH4III ACNGT 1 cut(s) 147
HpyCH4V TGCA 1 cut(s) 50
HpyF10VI GCNNNNNNNGC 1 cut(s) 314
HpyF3I CTNAG 1 cut(s) 89
Hsp92II CATG 4 cut(s) 50, 103, 112, 277
HspAI GCGC 1 cut(s) 86
LguI GCTCTTC 1 cut(s) 191
LpnPI CCDG 7 cut(s) 137, 141, 182, 285, 312, 318, 367
Lsp1109I GCAGC 1 cut(s) 148
MaeI CTAG 2 cut(s) 123, 252
MbiI CCGCTC 1 cut(s) 184
MboII GAAGA 3 cut(s) 63, 124, 178
MhlI GDGCHC 1 cut(s) 319
MluCI AATT 1 cut(s) 333
MnlI CCTC 2 cut(s) 232, 413
MseI TTAA 3 cut(s) 234, 342, 460
MspR9I CCNGG 1 cut(s) 300
MvaI CCWGG 1 cut(s) 300
MwoI GCNNNNNNNGC 1 cut(s) 314
NlaIII CATG 4 cut(s) 50, 103, 112, 277
PaeR7I CTCGAG 1 cut(s) 201
PagI TCATGA 1 cut(s) 99
PciSI GCTCTTC 1 cut(s) 191
PfeI GAWTC 2 cut(s) 96, 263
PflMI CCANNNNNTGG 1 cut(s) 381
PkrI GCNGC 1 cut(s) 163
Psp124BI GAGCTC 1 cut(s) 319
Psp6I CCWGG 1 cut(s) 298
PspGI CCWGG 1 cut(s) 298
PspPI GGNCC 1 cut(s) 302
PspXI VCTCGAGB 1 cut(s) 201
PstNI CAGNNNCTG 1 cut(s) 151
RsaI GTAC 2 cut(s) 118, 132
RsaNI GTAC 2 cut(s) 117, 131
SacI GAGCTC 1 cut(s) 319
SalI GTCGAC 1 cut(s) 139
SapI GCTCTTC 1 cut(s) 191
SaqAI TTAA 3 cut(s) 234, 342, 460
SatI GCNGC 1 cut(s) 162
Sau96I GGNCC 1 cut(s) 302
ScaI AGTACT 2 cut(s) 118, 132
ScrFI CCNGG 1 cut(s) 300
SduI GDGCHC 1 cut(s) 319
SetI ASST 7 cut(s) 18, 243, 273, 296, 304, 319, 405
Sfr274I CTCGAG 1 cut(s) 201
SinI GGWCC 1 cut(s) 302
SlaI CTCGAG 1 cut(s) 201
SmlI CTYRAG 1 cut(s) 201
SmoI CTYRAG 1 cut(s) 201
SpeI ACTAGT 1 cut(s) 122
Sse9I AATT 1 cut(s) 333
SsiI CCGC 1 cut(s) 182
SspMI CTAG 2 cut(s) 123, 252
SstI GAGCTC 1 cut(s) 319
StyD4I CCNGG 1 cut(s) 298
TaaI ACNGT 1 cut(s) 147
TaqI TCGA 2 cut(s) 140, 202
TasI AATT 1 cut(s) 333
TatI WGTACW 2 cut(s) 116, 130
TfiI GAWTC 2 cut(s) 96, 263
Tru1I TTAA 3 cut(s) 234, 342, 460
Tru9I TTAA 3 cut(s) 234, 342, 460
TscAI CASTG 1 cut(s) 386
TseI GCWGC 1 cut(s) 161
TspDTI ATGAA 5 cut(s) 17, 116, 125, 178, 203
TspGWI ACGGA 1 cut(s) 34
TspRI CASTG 1 cut(s) 386
Van91I CCANNNNNTGG 1 cut(s) 381
VpaK11BI GGWCC 1 cut(s) 302
XhoI CTCGAG 1 cut(s) 201
XmiI GTMKAC 1 cut(s) 140
XspI CTAG 2 cut(s) 123, 252
ZrmI AGTACT 2 cut(s) 118, 132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.