Rmu_sc0008462.1_g000004

F-box protein At3g07870-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0008462.1
OG0011619
Physical Location & Seq
Forward (+)
23400 .. 24197
798 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0008462.1_g000004.1.cds

Sequence Viewer

Length: 798 bp
atgagaattacagatgagaagaggttttatgtgtgcaacccggttacgggcgagttcatcactatcccgctacctaaggaaaagtatttagaatcggatgcatttgtgggacttggttttagcgctgtgaccaatgagtacaaggtgttgcagacgagcacctccgtttacgaccagggggagaaggaggctgagatttatacaatcggtagtagaggagtgtggagaagtattggaaaggctcctgtgcaacctgttgaattacctatgaattcttatttgcacggagctcttcattgggctccatcgctttcgattttttcttctacaggcgagtgtatacattctttcgactttgaaaaagaggagtttcgacggctacccctaccaagctgcgttgtacacgtggaggagtcatggtacgaggagaaggcatggaggttgggggtgctacaaggttgtctctttttaggtgtgccgggtgaagatcattataacgaatgtgacgtgtgggttatgaaggattacggtgttcaagagtcttggactaaagtttttgttttacaaaacttgctcctggatacattgtgtagaccatattttgaaccgcttatgtttttgagtgatgggagcattctaatatcatattgtaatgaagttttgatttcttataaccaagaaacggagagttacttgaaaatcgaaggttacttgggaaggataactgcttaccatccaagctttgtttccctttgcactgtttcaaatggagcggatgttgaaaggtactacttttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBA_1 FBA_3

Protein Analysis

265

Amino Acids

30.37

Weight (kDa)

4.81

Isoelectric Point (pI)

44.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 495, 672
AccBSI CCGCTC 1 cut(s) 773
AccI GTMKAC 2 cut(s) 340, 592
AciI CCGC 3 cut(s) 68, 608, 773
AcsI RAATTY 1 cut(s) 271
AcvI CACGTG 1 cut(s) 406
AfaI GTAC 4 cut(s) 140, 402, 422, 788
AfeI AGCGCT 1 cut(s) 124
AfiI CCNNNNNNNGG 3 cut(s) 46, 47, 682
AflIII ACRYGT 2 cut(s) 403, 507
AgsI TTSAA 7 cut(s) 260, 359, 536, 605, 697, 765, 782
AjiI CACGTC 1 cut(s) 508
AjnI CCWGG 2 cut(s) 174, 576
AjuI GAANNNNNNNTTGG 2 cut(s) 730, 762
AluBI AGCT 3 cut(s) 290, 393, 741
AluI AGCT 3 cut(s) 290, 393, 741
Alw21I GWGCWC 2 cut(s) 161, 292
Alw26I GTCTC 1 cut(s) 467
Aor51HI AGCGCT 1 cut(s) 124
ApeKI GCWGC 1 cut(s) 393
ApoI RAATTY 1 cut(s) 271
AspLEI GCGC 1 cut(s) 125
AsuC2I CCSGG 2 cut(s) 41, 480
AsuHPI GGTGA 1 cut(s) 494
AxyI CCTNAGG 1 cut(s) 75
BanII GRGCYC 2 cut(s) 292, 304
BbrPI CACGTG 1 cut(s) 406
Bbv12I GWGCWC 2 cut(s) 161, 292
BbvI GCAGC 1 cut(s) 380
BccI CCATC 3 cut(s) 313, 620, 741
BceAI ACGGC 1 cut(s) 392
BciT130I CCWGG 2 cut(s) 176, 578
BciVI GTATCC 1 cut(s) 574
BcnI CCSGG 2 cut(s) 41, 480
BcoDI GTCTC 1 cut(s) 467
BfmI CTRYAG 1 cut(s) 327
BfoI RGCGCY 1 cut(s) 126
BfuI GTATCC 1 cut(s) 574
BisI GCNGC 1 cut(s) 394
BlsI GCNGC 1 cut(s) 395
Bme1390I CCNGG 4 cut(s) 41, 176, 480, 578
BmgBI CACGTC 1 cut(s) 508
BmiI GGNNCC 2 cut(s) 243, 303
BmrFI CCNGG 4 cut(s) 41, 176, 480, 578
BmsI GCATC 1 cut(s) 88
BpuMI CCSGG 2 cut(s) 41, 480
BsaAI YACGTR 1 cut(s) 406
BsaJI CCNNGG 1 cut(s) 175
BsaXI ACNNNNNCTCC 4 cut(s) 404, 434, 762, 792
Bsc4I CCNNNNNNNGG 3 cut(s) 46, 47, 682
Bse21I CCTNAGG 1 cut(s) 75
BseBI CCWGG 2 cut(s) 176, 578
BseDI CCNNGG 1 cut(s) 175
BseGI GGATG 3 cut(s) 103, 733, 781
BseLI CCNNNNNNNGG 3 cut(s) 46, 47, 682
BseMII CTCAG 1 cut(s) 183
BseRI GAGGAG 4 cut(s) 231, 380, 425, 440
BseXI GCAGC 1 cut(s) 380
BsiHKAI GWGCWC 2 cut(s) 161, 292
BsiSI CCGG 2 cut(s) 41, 479
BslFI GGGAC 1 cut(s) 123
BslI CCNNNNNNNGG 3 cut(s) 46, 47, 682
BsmAI GTCTC 1 cut(s) 467
BsmFI GGGAC 1 cut(s) 123
BsmI GAATGC 1 cut(s) 633
Bsp1286I GDGCHC 3 cut(s) 161, 292, 304
Bsp1407I TGTACA 1 cut(s) 400
Bsp143I GATC 1 cut(s) 487
BspACI CCGC 3 cut(s) 68, 608, 773
BspCNI CTCAG 1 cut(s) 184
BspLI GGNNCC 2 cut(s) 243, 303
BspQI GCTCTTC 1 cut(s) 297
BsrBI CCGCTC 1 cut(s) 773
BsrGI TGTACA 1 cut(s) 400
BssECI CCNNGG 1 cut(s) 175
BssMI GATC 1 cut(s) 487
BssNAI GTATAC 1 cut(s) 341
Bst1107I GTATAC 1 cut(s) 341
Bst2UI CCWGG 2 cut(s) 176, 578
Bst4CI ACNGT 2 cut(s) 530, 760
Bst6I CTCTTC 2 cut(s) 14, 297
BstAUI TGTACA 1 cut(s) 400
BstBAI YACGTR 1 cut(s) 406
BstDEI CTNAG 2 cut(s) 75, 192
BstF5I GGATG 3 cut(s) 103, 733, 781
BstH2I RGCGCY 1 cut(s) 126
BstHHI GCGC 1 cut(s) 125
BstKTI GATC 1 cut(s) 490
BstMAI GTCTC 1 cut(s) 467
BstMBI GATC 1 cut(s) 487
BstNI CCWGG 2 cut(s) 176, 578
BstSCI CCNGG 4 cut(s) 39, 174, 478, 576
BstSFI CTRYAG 1 cut(s) 327
BstV1I GCAGC 1 cut(s) 380
BstZ17I GTATAC 1 cut(s) 341
Bsu36I CCTNAGG 1 cut(s) 75
BsuI GTATCC 1 cut(s) 574
BtgZI GCGATG 1 cut(s) 291
BtrI CACGTC 1 cut(s) 508
BtsCI GGATG 3 cut(s) 103, 733, 781
BtsIMutI CAGTG 1 cut(s) 756
CfoI GCGC 1 cut(s) 125
Csp6I GTAC 4 cut(s) 139, 401, 421, 787
CviAII CATG 2 cut(s) 417, 435
CviJI RGCY 7 cut(s) 191, 242, 290, 302, 379, 393, 741
CviKI_1 RGCY 7 cut(s) 191, 242, 290, 302, 379, 393, 741
CviQI GTAC 4 cut(s) 139, 401, 421, 787
DdeI CTNAG 2 cut(s) 75, 192
DpnI GATC 1 cut(s) 489
DpnII GATC 1 cut(s) 487
Eam1104I CTCTTC 2 cut(s) 14, 297
EarI CTCTTC 2 cut(s) 14, 297
Ecl136II GAGCTC 1 cut(s) 290
Eco24I GRGCYC 2 cut(s) 292, 304
Eco47III AGCGCT 1 cut(s) 124
Eco53kI GAGCTC 1 cut(s) 290
Eco72I CACGTG 1 cut(s) 406
Eco81I CCTNAGG 1 cut(s) 75
EcoICRI GAGCTC 1 cut(s) 290
EcoRI GAATTC 1 cut(s) 271
EcoRII CCWGG 2 cut(s) 174, 576
EcoT22I ATGCAT 1 cut(s) 103
EcoT38I GRGCYC 2 cut(s) 292, 304
FaeI CATG 2 cut(s) 420, 438
FaqI GGGAC 1 cut(s) 123
FatI CATG 2 cut(s) 416, 434
FauI CCCGC 1 cut(s) 75
FblI GTMKAC 2 cut(s) 340, 592
Fnu4HI GCNGC 1 cut(s) 394
FokI GGATG 3 cut(s) 110, 720, 788
FriOI GRGCYC 2 cut(s) 292, 304
Fsp4HI GCNGC 1 cut(s) 394
GlaI GCGC 1 cut(s) 124
GluI GCNGC 1 cut(s) 394
HaeII RGCGCY 1 cut(s) 126
HapII CCGG 2 cut(s) 41, 479
HhaI GCGC 1 cut(s) 125
Hin1II CATG 2 cut(s) 420, 438
Hin6I GCGC 1 cut(s) 123
HinP1I GCGC 1 cut(s) 123
HindIII AAGCTT 1 cut(s) 739
HinfI GANTC 3 cut(s) 92, 413, 539
HpaII CCGG 2 cut(s) 41, 479
HphI GGTGA 1 cut(s) 494
Hpy166II GTNNAC 4 cut(s) 169, 341, 403, 593
Hpy188I TCNGA 1 cut(s) 97
Hpy188III TCNNGA 1 cut(s) 536
Hpy8I GTNNAC 4 cut(s) 169, 341, 403, 593
Hpy99I CGWCG 1 cut(s) 378
HpyAV CCTTC 5 cut(s) 178, 424, 514, 698, 711
HpyCH4III ACNGT 2 cut(s) 530, 760
HpyCH4IV ACGT 2 cut(s) 405, 507
HpyCH4V TGCA 6 cut(s) 36, 101, 151, 250, 283, 756
HpyF3I CTNAG 2 cut(s) 75, 192
HpySE526I ACGT 2 cut(s) 405, 507
Hsp92II CATG 2 cut(s) 420, 438
HspAI GCGC 1 cut(s) 123
Kzo9I GATC 1 cut(s) 487
LguI GCTCTTC 1 cut(s) 297
LmnI GCTCC 6 cut(s) 247, 287, 307, 579, 630, 770
LpnPI CCDG 9 cut(s) 54, 161, 188, 258, 267, 315, 492, 563, 590
Lsp1109I GCAGC 1 cut(s) 380
LweI GCATC 1 cut(s) 88
MaeII ACGT 2 cut(s) 405, 507
MaeIII GTNAC 5 cut(s) 43, 127, 503, 689, 707
MalI GATC 1 cut(s) 489
MbiI CCGCTC 1 cut(s) 773
MboI GATC 1 cut(s) 487
MboII GAAGA 4 cut(s) 31, 284, 315, 497
MhlI GDGCHC 3 cut(s) 161, 292, 304
MluCI AATT 3 cut(s) 6, 260, 271
MlyI GAGTC 2 cut(s) 422, 548
MnlI CCTC 8 cut(s) 15, 172, 181, 209, 358, 403, 418, 432
Mph1103I ATGCAT 1 cut(s) 103
MspI CCGG 2 cut(s) 41, 479
MspR9I CCNGG 4 cut(s) 41, 176, 480, 578
Mva1269I GAATGC 1 cut(s) 633
MvaI CCWGG 2 cut(s) 176, 578
NciI CCSGG 2 cut(s) 41, 480
NdeII GATC 1 cut(s) 487
NlaIII CATG 2 cut(s) 420, 438
NlaIV GGNNCC 2 cut(s) 243, 303
NmuCI GTSAC 2 cut(s) 127, 503
NsiI ATGCAT 1 cut(s) 103
PciSI GCTCTTC 1 cut(s) 297
PcsI WCGNNNNNNNCGW 1 cut(s) 504
PctI GAATGC 1 cut(s) 633
PfeI GAWTC 1 cut(s) 92
PfoI TCCNGGA 1 cut(s) 576
PkrI GCNGC 1 cut(s) 395
PleI GAGTC 2 cut(s) 421, 547
PmaCI CACGTG 1 cut(s) 406
PmlI CACGTG 1 cut(s) 406
PpsI GAGTC 2 cut(s) 421, 547
Ppu21I YACGTR 1 cut(s) 406
PsiI TTATAA 2 cut(s) 495, 672
Psp124BI GAGCTC 1 cut(s) 292
Psp6I CCWGG 2 cut(s) 174, 576
PspCI CACGTG 1 cut(s) 406
PspGI CCWGG 2 cut(s) 174, 576
PspN4I GGNNCC 2 cut(s) 243, 303
RsaI GTAC 4 cut(s) 140, 402, 422, 788
RsaNI GTAC 4 cut(s) 139, 401, 421, 787
SacI GAGCTC 1 cut(s) 292
SapI GCTCTTC 1 cut(s) 297
SatI GCNGC 1 cut(s) 394
Sau3AI GATC 1 cut(s) 487
SchI GAGTC 2 cut(s) 422, 548
ScrFI CCNGG 4 cut(s) 41, 176, 480, 578
SduI GDGCHC 3 cut(s) 161, 292, 304
SfaNI GCATC 1 cut(s) 88
SfcI CTRYAG 1 cut(s) 327
Sse9I AATT 3 cut(s) 6, 260, 271
SsiI CCGC 3 cut(s) 68, 608, 773
SstI GAGCTC 1 cut(s) 292
StyD4I CCNGG 4 cut(s) 39, 174, 478, 576
TaaI ACNGT 2 cut(s) 530, 760
TaiI ACGT 2 cut(s) 408, 510
TaqI TCGA 4 cut(s) 314, 351, 373, 702
TasI AATT 3 cut(s) 6, 260, 271
TatI WGTACW 2 cut(s) 138, 400
TfiI GAWTC 1 cut(s) 92
TscAI CASTG 1 cut(s) 763
TseFI GTSAC 2 cut(s) 127, 503
TseI GCWGC 1 cut(s) 393
Tsp45I GTSAC 2 cut(s) 127, 503
TspDTI ATGAA 5 cut(s) 46, 284, 284, 533, 669
TspGWI ACGGA 3 cut(s) 154, 300, 698
TspRI CASTG 1 cut(s) 763
XapI RAATTY 1 cut(s) 271
XmiI GTMKAC 2 cut(s) 340, 592
Zsp2I ATGCAT 1 cut(s) 103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.