Rmu_ssc0000028.1_g000003

GDP-mannose 3,5-epimerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000028.1
OG0003420
Physical Location & Seq
Reverse (-)
9447 .. 15648
6202 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1878 bp
atggttgaagttctccaaaacactcccaaacggagaggatctagaaaatttggcaaatgtcccacactgagcagttgcacagttgaatacttgtttgagacagaaagaattgagttagatatgttagtcgttacacatgcttctctcctaaagctttttgatattgctgatacatggatcttttcggaaatattgactgaattaaggattttccatgagaagttggtttcattgatcaattcattgtcacaggaaaacaacaagtttctcattaaggtatcaaatacaaagaatatattgccaactgatcactttgagttgaacaccaacttcacattcacttcccctatcccccaaccaatactggaggataacaggaggaggaatgtggtggagagagtaaaccatgacatgagcttgatcatccagtgcatgcttatgaaggtaatgaaacccagtataggttttggacagatggataggcagaagctagcaattgcagcaattgaaaagtgtattgggttagaaaaattcaggtttgttccaacaagagcagctgttggtgttagcattgcaagtctgatcaagaacgagtatttgctggtggataagaacgacaacaacatcagtctatatatagccgagtgttacagggccagaactcacacctccatctctgagaaccctttacctcacatcttcttcttcttcttcaaccttgaagcaccaaactttacgcatttcagtatgggaagtgctggtgaaagtggttatggtgcatacacctatgaggctcttgagagggagccttattggccctcagaaaaactccgaatttccattactggggctggtggctttattgcctcccacattgcccgccgattgaagaatgagggccactacattattgcctctgactggaagaagaatgagcacatgagtgaagacatgttctgccatgaattccatcttgcggacctcagggtcatggataactgcttgaaggttaccaaggatgtcgatcatgtgttcaaccttgctgctgatatgggcggcatgggattcattcagtccaaccactctgtcattatgtacaataataccatgatcagctttaacatgctggaagctgctaggatcagtggagtgaaaaggttattctatgcttccagtgcttgtatttatcctgagtttaagcagctggaaaccaatgtgagcttgaaggaggctgatgcctggcctgcagagcctcaagatgcttatggcttagagaaacttgcaactgaggagttgtgcaagcactacaccaaagactttggaatcgaatgccgtattggaaggttccacaacatctatggtcctttcggaacctggaaagggggcagggagaaggctcctgctgctttctgcagaaaggctctcacttccactgataagtttgagatgtggggagatggactccagacccgatccttcacctttattgatgaatgtgttgaaggtgtactccggttgacaaagtccgacttccgtgagccagtgaatattggaagcgatgagatggttagcatgaatgagatggctgagattgttcttagctttgagaacaagaagctgcctattcaacacattcctgggcctgagggtgtccgtggtcgtaactctgacaacaccctgatcaaagagaaacttggctgggctcctaccatgaggctgaaggatggactgaggtttacatacttctggatcaaggaacagattgagaaagagaaagcacaaggtactgacctgtcgctttatgggtcatctaaggtggtgggaactcaagccccagttcaacttggttcacttcgtgccgctgacggcaaagagtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 ko00520 ko01100 ko01110 map00053 map00520 map01100 map01110
Pfam Domains
Protein Families
Epimerase

Protein Analysis

625

Amino Acids

71.09

Weight (kDa)

6.52

Isoelectric Point (pI)

42.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin_AB PF26557 15 - 111 2.7e-08 Cullin alpha+beta domain
Epimerase PF01370 279 - 518 7.5e-45 NAD dependent epimerase/dehydratase family
GDP_Man_Dehyd PF16363 281 - 578 4.4e-26 GDP-mannose 4,6 dehydratase
3Beta_HSD PF01073 281 - 433 4.2e-09 3-beta hydroxysteroid dehydrogenase/isomerase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 986
AciI CCGC 4 cut(s) 880, 977, 1056, 1860
AclWI GGATC 5 cut(s) 46, 185, 1148, 1467, 1757
AcsI RAATTY 4 cut(s) 47, 530, 834, 965
AcuI CTGAAG 1 cut(s) 1739
AdeI CACNNNGTG 1 cut(s) 1856
AfaI GTAC 3 cut(s) 1097, 1509, 1786
AfiI CCNNNNNNNGG 5 cut(s) 461, 846, 921, 976, 1380
AflIII ACRYGT 1 cut(s) 951
AjnI CCWGG 3 cut(s) 1238, 1373, 1636
AjuI GAANNNNNNNTTGG 6 cut(s) 320, 352, 501, 533, 1320, 1352
AleI CACNNNNGTG 1 cut(s) 942
Alw21I GWGCWC 1 cut(s) 939
Alw26I GTCTC 1 cut(s) 92
AlwI GGATC 5 cut(s) 46, 185, 1148, 1467, 1757
AoxI GGCC 5 cut(s) 654, 815, 898, 1241, 1640
ApeKI GCWGC 7 cut(s) 500, 554, 1043, 1133, 1201, 1403, 1618
ApoI RAATTY 4 cut(s) 47, 530, 834, 965
AspS9I GGNCC 6 cut(s) 654, 816, 898, 979, 1361, 1640
AsuHPI GGTGA 2 cut(s) 773, 1471
AsuNHI GCTAGC 1 cut(s) 490
AvaII GGWCC 2 cut(s) 979, 1361
AxyI CCTNAGG 2 cut(s) 983, 1644
BanII GRGCYC 1 cut(s) 1705
BbsI GAAGAC 1 cut(s) 954
Bbv12I GWGCWC 1 cut(s) 939
BbvI GCAGC 7 cut(s) 512, 566, 1030, 1120, 1213, 1390, 1605
BccI CCATC 7 cut(s) 469, 680, 978, 1451, 1558, 1576, 1718
BceAI ACGGC 1 cut(s) 1317
BciT130I CCWGG 3 cut(s) 1240, 1375, 1638
BclI TGATCA 6 cut(s) 234, 307, 420, 582, 1110, 1680
BcoDI GTCTC 1 cut(s) 92
BfaI CTAG 3 cut(s) 42, 491, 1137
BfmI CTRYAG 2 cut(s) 1245, 1411
BglI GCCNNNNNGGC 1 cut(s) 814
BisI GCNGC 9 cut(s) 501, 555, 1044, 1057, 1134, 1202, 1404, 1619, 1860
BlsI GCNGC 9 cut(s) 502, 556, 1045, 1058, 1135, 1203, 1405, 1620, 1861
Bme1390I CCNGG 3 cut(s) 1240, 1375, 1638
Bme18I GGWCC 2 cut(s) 979, 1361
BmgT120I GGNCC 6 cut(s) 654, 816, 898, 979, 1361, 1640
BmiI GGNNCC 5 cut(s) 807, 1346, 1372, 1398, 1704
BmrFI CCNGG 3 cut(s) 1240, 1375, 1638
BmrI ACTGGG 3 cut(s) 450, 855, 1829
BmsI GCATC 2 cut(s) 1225, 1249
BmtI GCTAGC 1 cut(s) 494
BmuI ACTGGG 3 cut(s) 450, 855, 1829
BpiI GAAGAC 1 cut(s) 954
BplI GAGNNNNNCTC 2 cut(s) 1446, 1478
BpmI CTGGAG 2 cut(s) 386, 1448
BpuEI CTTGAG 3 cut(s) 818, 1239, 1812
BsaBI GATNNNNATC 1 cut(s) 1023
BsaJI CCNNGG 3 cut(s) 1014, 1637, 1654
BsaWI WCCGGW 1 cut(s) 1512
Bsc4I CCNNNNNNNGG 5 cut(s) 461, 846, 921, 976, 1380
Bse1I ACTGG 8 cut(s) 369, 427, 456, 850, 926, 1173, 1541, 1835
Bse21I CCTNAGG 2 cut(s) 983, 1644
Bse3DI GCAATG 2 cut(s) 570, 873
Bse8I GATNNNNATC 1 cut(s) 1023
BseBI CCWGG 3 cut(s) 1240, 1375, 1638
BseDI CCNNGG 3 cut(s) 1014, 1637, 1654
BseGI GGATG 3 cut(s) 423, 1024, 1729
BseJI GATNNNNATC 1 cut(s) 1023
BseLI CCNNNNNNNGG 5 cut(s) 461, 846, 921, 976, 1380
BseMI GCAATG 2 cut(s) 570, 873
BseMII CTCAG 9 cut(s) 59, 669, 834, 997, 1182, 1278, 1578, 1635, 1721
BseNI ACTGG 8 cut(s) 369, 427, 456, 850, 926, 1173, 1541, 1835
BseRI GAGGAG 2 cut(s) 394, 1304
BseXI GCAGC 7 cut(s) 512, 566, 1030, 1120, 1213, 1390, 1605
BseYI CCCAGC 1 cut(s) 1698
BshFI GGCC 5 cut(s) 656, 817, 900, 1243, 1642
BsiHKAI GWGCWC 1 cut(s) 939
BsiSI CCGG 1 cut(s) 1513
BslFI GGGAC 1 cut(s) 45
BslI CCNNNNNNNGG 5 cut(s) 461, 846, 921, 976, 1380
BsmAI GTCTC 1 cut(s) 92
BsmFI GGGAC 1 cut(s) 45
BsmI GAATGC 1 cut(s) 1334
BsnI GGCC 5 cut(s) 656, 817, 900, 1243, 1642
Bsp1286I GDGCHC 2 cut(s) 939, 1705
Bsp1407I TGTACA 1 cut(s) 1095
BspACI CCGC 4 cut(s) 880, 977, 1056, 1860
BspANI GGCC 5 cut(s) 656, 817, 900, 1243, 1642
BspCNI CTCAG 9 cut(s) 60, 670, 833, 996, 1183, 1279, 1579, 1636, 1722
BspLI GGNNCC 5 cut(s) 807, 1346, 1372, 1398, 1704
BspMAI CTGCAG 2 cut(s) 1249, 1415
BspOI GCTAGC 1 cut(s) 494
BspPI GGATC 5 cut(s) 46, 185, 1148, 1467, 1757
BsrDI GCAATG 2 cut(s) 570, 873
BsrGI TGTACA 1 cut(s) 1095
BsrI ACTGG 8 cut(s) 369, 427, 456, 850, 926, 1173, 1541, 1835
BssECI CCNNGG 3 cut(s) 1014, 1637, 1654
BssT1I CCWWGG 1 cut(s) 1014
Bst2UI CCWGG 3 cut(s) 1240, 1375, 1638
Bst4CI ACNGT 1 cut(s) 82
BstAUI TGTACA 1 cut(s) 1095
BstC8I GCNNGC 5 cut(s) 434, 492, 880, 1245, 1301
BstDSI CCRYGG 1 cut(s) 1654
BstEII GGTNACC 1 cut(s) 1009
BstF5I GGATG 3 cut(s) 423, 1024, 1729
BstMAI GTCTC 1 cut(s) 92
BstMWI GCNNNNNNNGC 6 cut(s) 500, 814, 1175, 1244, 1249, 1403
BstNI CCWGG 3 cut(s) 1240, 1375, 1638
BstNSI RCATGY 4 cut(s) 140, 436, 955, 1126
BstPI GGTNACC 1 cut(s) 1009
BstSCI CCNGG 3 cut(s) 1238, 1373, 1636
BstSFI CTRYAG 2 cut(s) 1245, 1411
BstV1I GCAGC 7 cut(s) 512, 566, 1030, 1120, 1213, 1390, 1605
BstV2I GAAGAC 1 cut(s) 954
BstX2I RGATCY 2 cut(s) 38, 177
BstYI RGATCY 2 cut(s) 38, 177
Bsu36I CCTNAGG 2 cut(s) 983, 1644
BsuRI GGCC 5 cut(s) 656, 817, 900, 1243, 1642
BtgI CCRYGG 1 cut(s) 1654
BtgZI GCGATG 1 cut(s) 1572
BtsCI GGATG 3 cut(s) 423, 1024, 1729
BtsIMutI CAGTG 6 cut(s) 65, 434, 1150, 1180, 1431, 1548
Cac8I GCNNGC 5 cut(s) 434, 492, 880, 1245, 1301
Cfr13I GGNCC 6 cut(s) 654, 816, 898, 979, 1361, 1640
Csp6I GTAC 3 cut(s) 1096, 1508, 1785
CviQI GTAC 3 cut(s) 1096, 1508, 1785
DraIII CACNNNGTG 1 cut(s) 1856
DrdI GACNNNNNNGTC 1 cut(s) 986
DseDI GACNNNNNNGTC 1 cut(s) 986
Eco130I CCWWGG 1 cut(s) 1014
Eco24I GRGCYC 1 cut(s) 1705
Eco47I GGWCC 2 cut(s) 979, 1361
Eco57I CTGAAG 1 cut(s) 1739
Eco81I CCTNAGG 2 cut(s) 983, 1644
Eco91I GGTNACC 1 cut(s) 1009
EcoO65I GGTNACC 1 cut(s) 1009
EcoRI GAATTC 1 cut(s) 965
EcoRII CCWGG 3 cut(s) 1238, 1373, 1636
EcoT14I CCWWGG 1 cut(s) 1014
EcoT38I GRGCYC 1 cut(s) 1705
ErhI CCWWGG 1 cut(s) 1014
FalI AAGNNNNNCTT 4 cut(s) 1514, 1546, 1677, 1709
FaqI GGGAC 1 cut(s) 45
FauI CCCGC 1 cut(s) 887
FbaI TGATCA 6 cut(s) 234, 307, 420, 582, 1110, 1680
Fnu4HI GCNGC 9 cut(s) 501, 555, 1044, 1057, 1134, 1202, 1404, 1619, 1860
FokI GGATG 3 cut(s) 410, 1031, 1736
FriOI GRGCYC 1 cut(s) 1705
Fsp4HI GCNGC 9 cut(s) 501, 555, 1044, 1057, 1134, 1202, 1404, 1619, 1860
FspBI CTAG 3 cut(s) 42, 491, 1137
GluI GCNGC 9 cut(s) 501, 555, 1044, 1057, 1134, 1202, 1404, 1619, 1860
GsaI CCCAGC 1 cut(s) 1702
GsuI CTGGAG 2 cut(s) 386, 1448
HaeIII GGCC 5 cut(s) 656, 817, 900, 1243, 1642
HapII CCGG 1 cut(s) 1513
HincII GTYRAC 1 cut(s) 1518
HindII GTYRAC 1 cut(s) 1518
HindIII AAGCTT 1 cut(s) 152
HinfI GANTC 3 cut(s) 1065, 1323, 1461
HpaII CCGG 1 cut(s) 1513
HphI GGTGA 2 cut(s) 773, 1471
Hpy166II GTNNAC 5 cut(s) 403, 1508, 1518, 1737, 1850
Hpy188I TCNGA 9 cut(s) 187, 582, 679, 823, 833, 919, 1370, 1528, 1669
Hpy188III TCNNGA 7 cut(s) 42, 586, 797, 1190, 1256, 1465, 1747
Hpy8I GTNNAC 5 cut(s) 403, 1508, 1518, 1737, 1850
HpyAV CCTTC 8 cut(s) 436, 1000, 1219, 1335, 1387, 1486, 1496, 1714
HpyCH4III ACNGT 1 cut(s) 82
HpyCH4V TGCA 9 cut(s) 78, 432, 500, 575, 779, 1247, 1283, 1299, 1413
HpyF10VI GCNNNNNNNGC 6 cut(s) 500, 814, 1175, 1244, 1249, 1403
Ksp22I TGATCA 6 cut(s) 234, 307, 420, 582, 1110, 1680
LmnI GCTCC 3 cut(s) 805, 1402, 1708
Lsp1109I GCAGC 7 cut(s) 512, 566, 1030, 1120, 1213, 1390, 1605
LweI GCATC 2 cut(s) 1225, 1249
MaeI CTAG 3 cut(s) 42, 491, 1137
MaeIII GTNAC 5 cut(s) 130, 246, 647, 1009, 1661
MboII GAAGA 9 cut(s) 691, 694, 697, 700, 703, 901, 937, 940, 959
MfeI CAATTG 2 cut(s) 495, 504
MflI RGATCY 2 cut(s) 38, 177
MhlI GDGCHC 2 cut(s) 939, 1705
MluCI AATT 9 cut(s) 47, 108, 200, 238, 495, 504, 530, 834, 965
MlyI GAGTC 1 cut(s) 1455
MmeI TCCRAC 3 cut(s) 569, 1101, 1551
MseI TTAA 4 cut(s) 203, 273, 1119, 1197
MslI CAYNNNNRTG 2 cut(s) 437, 942
MspA1I CMGCKG 3 cut(s) 557, 1204, 1862
MspI CCGG 1 cut(s) 1513
MspR9I CCNGG 3 cut(s) 1240, 1375, 1638
MunI CAATTG 2 cut(s) 495, 504
Mva1269I GAATGC 1 cut(s) 1334
MvaI CCWGG 3 cut(s) 1240, 1375, 1638
MwoI GCNNNNNNNGC 6 cut(s) 500, 814, 1175, 1244, 1249, 1403
NheI GCTAGC 1 cut(s) 490
NlaIV GGNNCC 5 cut(s) 807, 1346, 1372, 1398, 1704
NmeAIII GCCGAG 1 cut(s) 667
NmuCI GTSAC 1 cut(s) 246
NspI RCATGY 4 cut(s) 140, 436, 955, 1126
OliI CACNNNNGTG 1 cut(s) 942
PaeI GCATGC 1 cut(s) 436
PciI ACATGT 1 cut(s) 951
PctI GAATGC 1 cut(s) 1334
PfeI GAWTC 2 cut(s) 1065, 1323
PflFI GACNNNGTC 1 cut(s) 1522
PkrI GCNGC 9 cut(s) 502, 556, 1045, 1058, 1135, 1203, 1405, 1620, 1861
PleI GAGTC 1 cut(s) 1455
PpsI GAGTC 1 cut(s) 1455
PscI ACATGT 1 cut(s) 951
Psp6I CCWGG 3 cut(s) 1238, 1373, 1636
PspEI GGTNACC 1 cut(s) 1009
PspFI CCCAGC 1 cut(s) 1698
PspGI CCWGG 3 cut(s) 1238, 1373, 1636
PspN4I GGNNCC 5 cut(s) 807, 1346, 1372, 1398, 1704
PspPI GGNCC 6 cut(s) 654, 816, 898, 979, 1361, 1640
PstI CTGCAG 2 cut(s) 1249, 1415
PsuI RGATCY 2 cut(s) 38, 177
PsyI GACNNNGTC 1 cut(s) 1522
PvuII CAGCTG 2 cut(s) 557, 1204
RsaI GTAC 3 cut(s) 1097, 1509, 1786
RsaNI GTAC 3 cut(s) 1096, 1508, 1785
RseI CAYNNNNRTG 2 cut(s) 437, 942
SaqAI TTAA 4 cut(s) 203, 273, 1119, 1197
SatI GCNGC 9 cut(s) 501, 555, 1044, 1057, 1134, 1202, 1404, 1619, 1860
Sau96I GGNCC 6 cut(s) 654, 816, 898, 979, 1361, 1640
SchI GAGTC 1 cut(s) 1455
ScrFI CCNGG 3 cut(s) 1240, 1375, 1638
SduI GDGCHC 2 cut(s) 939, 1705
SfaNI GCATC 2 cut(s) 1225, 1249
SfcI CTRYAG 2 cut(s) 1245, 1411
SinI GGWCC 2 cut(s) 979, 1361
SmiMI CAYNNNNRTG 2 cut(s) 437, 942
SmlI CTYRAG 3 cut(s) 797, 1254, 1827
SmoI CTYRAG 3 cut(s) 797, 1254, 1827
SphI GCATGC 1 cut(s) 436
Sse9I AATT 9 cut(s) 47, 108, 200, 238, 495, 504, 530, 834, 965
SsiI CCGC 4 cut(s) 880, 977, 1056, 1860
SspI AATATT 2 cut(s) 192, 1549
SspMI CTAG 3 cut(s) 42, 491, 1137
StyD4I CCNGG 3 cut(s) 1238, 1373, 1636
StyI CCWWGG 1 cut(s) 1014
TaaI ACNGT 1 cut(s) 82
TaqI TCGA 2 cut(s) 1023, 1326
TasI AATT 9 cut(s) 47, 108, 200, 238, 495, 504, 530, 834, 965
TatI WGTACW 2 cut(s) 1095, 1507
TauI GCSGC 2 cut(s) 1059, 1862
TfiI GAWTC 2 cut(s) 1065, 1323
Tru1I TTAA 4 cut(s) 203, 273, 1119, 1197
Tru9I TTAA 4 cut(s) 203, 273, 1119, 1197
TscAI CASTG 6 cut(s) 72, 434, 1150, 1180, 1438, 1548
TseFI GTSAC 1 cut(s) 246
TseI GCWGC 7 cut(s) 500, 554, 1043, 1133, 1201, 1403, 1618
Tsp45I GTSAC 1 cut(s) 246
TspDTI ATGAA 8 cut(s) 219, 231, 455, 464, 978, 1057, 1506, 1589
TspGWI ACGGA 3 cut(s) 46, 1523, 1643
TspRI CASTG 6 cut(s) 72, 434, 1150, 1180, 1438, 1548
Tth111I GACNNNGTC 1 cut(s) 1522
VpaK11BI GGWCC 2 cut(s) 979, 1361
XapI RAATTY 4 cut(s) 47, 530, 834, 965
XbaI TCTAGA 1 cut(s) 41
XceI RCATGY 4 cut(s) 140, 436, 955, 1126
XcmI CCANNNNNNNNNTGG 1 cut(s) 1355
XspI CTAG 3 cut(s) 42, 491, 1137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.