Rmu_ssc0000050.1_g000102

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000050.1
OG0006082
Physical Location & Seq
Forward (+)
480643 .. 481164
522 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000050.1_g000102.1.cds

Sequence Viewer

Length: 522 bp
atgtccgctacacctatggaccagctgcaaccaccaccaccacccgtttatgtgacccaacaagcctacaagacccattcgggtcacggctcggtcgggcccgtgattgcggtgctggcagtgatcaccatcctcggagtcatagcgggaatgataggtcggctttgctcaggtcgtcgggtcatgggtcatggacaatactacgacttcgaagcatggctcgagaagagatgctcgtcttgcattgacggtaggatcgaacctccgcctccgcctccgccgccccatattccggctaccgcctccgtcgaggaggttcaggcggccgagctggtggttgagaaccgacaagagatgaaggaagaagaacaagtacaacctcagcagcagcaaaaacaacgtcaaaatcaacaacaacaacgccaacagcagcagcaacaacaacagcagcagcagcaacaacagcagcagcagcgacaacaacaagaacagaattcacaggaaagtagtggtgagacttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

173

Amino Acids

19.52

Weight (kDa)

5.55

Isoelectric Point (pI)

93.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 9 cut(s) 6, 110, 146, 266, 272, 278, 281, 300, 323
AclWI GGATC 1 cut(s) 263
AcoI YGGCCR 1 cut(s) 324
AcsI RAATTY 1 cut(s) 493
AfaI GTAC 1 cut(s) 375
AfiI CCNNNNNNNGG 1 cut(s) 292
AluBI AGCT 2 cut(s) 25, 331
AluI AGCT 2 cut(s) 25, 331
Alw26I GTCTC 1 cut(s) 509
AlwI GGATC 1 cut(s) 263
Ama87I CYCGRG 1 cut(s) 221
AoxI GGCC 2 cut(s) 98, 324
ApaI GGGCCC 1 cut(s) 102
ApoI RAATTY 1 cut(s) 493
ArsI GACNNNNNNTTYG 2 cut(s) 385, 417
AspS9I GGNCC 3 cut(s) 19, 98, 99
AsuHPI GGTGA 1 cut(s) 118
AsuII TTCGAA 1 cut(s) 210
AvaI CYCGRG 1 cut(s) 221
AvaII GGWCC 1 cut(s) 19
BaeGI GKGCMC 1 cut(s) 102
BanII GRGCYC 1 cut(s) 102
BarI GAAGNNNNNNTAC 2 cut(s) 357, 389
BbvCI CCTCAGC 1 cut(s) 381
BccI CCATC 1 cut(s) 137
BceAI ACGGC 1 cut(s) 103
BclI TGATCA 1 cut(s) 123
BcoDI GTCTC 1 cut(s) 509
Bme18I GGWCC 1 cut(s) 19
BmeT110I CYCGRG 1 cut(s) 221
BmgT120I GGNCC 3 cut(s) 19, 98, 99
BmiI GGNNCC 1 cut(s) 100
BmsI GCATC 1 cut(s) 221
Bpu10I CCTNAGC 2 cut(s) 169, 381
Bpu14I TTCGAA 1 cut(s) 210
BsaBI GATNNNNATC 1 cut(s) 128
BsaJI CCNNGG 1 cut(s) 133
Bsc4I CCNNNNNNNGG 1 cut(s) 292
Bse8I GATNNNNATC 1 cut(s) 128
BseDI CCNNGG 1 cut(s) 133
BseGI GGATG 1 cut(s) 129
BseJI GATNNNNATC 1 cut(s) 128
BseLI CCNNNNNNNGG 1 cut(s) 292
BseMII CTCAG 2 cut(s) 183, 395
BseRI GAGGAG 1 cut(s) 326
BseSI GKGCMC 1 cut(s) 102
BseX3I CGGCCG 1 cut(s) 324
Bsh1285I CGRYCG 2 cut(s) 96, 327
BshFI GGCC 2 cut(s) 100, 326
BsiEI CGRYCG 2 cut(s) 96, 327
BsiHKCI CYCGRG 1 cut(s) 221
BsiSI CCGG 1 cut(s) 293
BslI CCNNNNNNNGG 1 cut(s) 292
BsmAI GTCTC 1 cut(s) 509
BsnI GGCC 2 cut(s) 100, 326
BsoBI CYCGRG 1 cut(s) 221
Bsp119I TTCGAA 1 cut(s) 210
Bsp120I GGGCCC 1 cut(s) 98
Bsp1286I GDGCHC 1 cut(s) 102
Bsp143I GATC 2 cut(s) 123, 255
BspACI CCGC 9 cut(s) 6, 110, 146, 266, 272, 278, 281, 300, 323
BspANI GGCC 2 cut(s) 100, 326
BspCNI CTCAG 2 cut(s) 182, 394
BspLI GGNNCC 1 cut(s) 100
BspPI GGATC 1 cut(s) 263
BspT104I TTCGAA 1 cut(s) 210
BssECI CCNNGG 1 cut(s) 133
BssMI GATC 2 cut(s) 123, 255
Bst4CI ACNGT 1 cut(s) 251
Bst6I CTCTTC 1 cut(s) 221
BstBI TTCGAA 1 cut(s) 210
BstC8I GCNNGC 1 cut(s) 117
BstDEI CTNAG 2 cut(s) 169, 381
BstF5I GGATG 1 cut(s) 129
BstKTI GATC 2 cut(s) 126, 258
BstMAI GTCTC 1 cut(s) 509
BstMBI GATC 2 cut(s) 123, 255
BstMCI CGRYCG 2 cut(s) 96, 327
BstMWI GCNNNNNNNGC 6 cut(s) 116, 240, 280, 454, 463, 472
BstSLI GKGCMC 1 cut(s) 102
BstZI CGGCCG 1 cut(s) 324
BsuRI GGCC 2 cut(s) 100, 326
BtsCI GGATG 1 cut(s) 129
BtsI GCAGTG 1 cut(s) 126
BtsIMutI CAGTG 1 cut(s) 126
Cac8I GCNNGC 1 cut(s) 117
Cfr13I GGNCC 3 cut(s) 19, 98, 99
Csp6I GTAC 1 cut(s) 374
CviAII CATG 3 cut(s) 184, 191, 216
CviJI RGCY 9 cut(s) 25, 65, 90, 100, 163, 220, 296, 326, 331
CviKI_1 RGCY 9 cut(s) 25, 65, 90, 100, 163, 220, 296, 326, 331
CviQI GTAC 1 cut(s) 374
DdeI CTNAG 2 cut(s) 169, 381
DpnI GATC 2 cut(s) 125, 257
DpnII GATC 2 cut(s) 123, 255
EaeI YGGCCR 1 cut(s) 324
EagI CGGCCG 1 cut(s) 324
Eam1104I CTCTTC 1 cut(s) 221
EarI CTCTTC 1 cut(s) 221
EciI GGCGGA 3 cut(s) 255, 261, 267
EclXI CGGCCG 1 cut(s) 324
Eco24I GRGCYC 1 cut(s) 102
Eco47I GGWCC 1 cut(s) 19
Eco52I CGGCCG 1 cut(s) 324
Eco88I CYCGRG 1 cut(s) 221
EcoRI GAATTC 1 cut(s) 493
EcoT38I GRGCYC 1 cut(s) 102
FaeI CATG 3 cut(s) 187, 194, 219
FaiI YATR 7 cut(s) 17, 51, 143, 185, 192, 217, 288
FatI CATG 3 cut(s) 183, 190, 215
FauI CCCGC 1 cut(s) 139
FbaI TGATCA 1 cut(s) 123
FokI GGATG 1 cut(s) 116
FriOI GRGCYC 1 cut(s) 102
HaeIII GGCC 2 cut(s) 100, 326
HapII CCGG 1 cut(s) 293
Hin1II CATG 3 cut(s) 187, 194, 219
HinfI GANTC 1 cut(s) 138
HpaII CCGG 1 cut(s) 293
HphI GGTGA 1 cut(s) 118
Hpy188I TCNGA 1 cut(s) 137
Hpy188III TCNNGA 1 cut(s) 223
Hpy99I CGWCG 2 cut(s) 180, 311
HpyAV CCTTC 1 cut(s) 352
HpyCH4III ACNGT 1 cut(s) 251
HpyCH4IV ACGT 1 cut(s) 400
HpyCH4V TGCA 2 cut(s) 28, 243
HpyF10VI GCNNNNNNNGC 6 cut(s) 116, 240, 280, 454, 463, 472
HpyF3I CTNAG 2 cut(s) 169, 381
HpySE526I ACGT 1 cut(s) 400
Hsp92II CATG 3 cut(s) 187, 194, 219
Ksp22I TGATCA 1 cut(s) 123
Kzo9I GATC 2 cut(s) 123, 255
LpnPI CCDG 7 cut(s) 35, 101, 156, 305, 306, 317, 485
LweI GCATC 1 cut(s) 221
MaeII ACGT 1 cut(s) 400
MaeIII GTNAC 2 cut(s) 52, 83
MalI GATC 2 cut(s) 125, 257
MboI GATC 2 cut(s) 123, 255
MboII GAAGA 3 cut(s) 238, 374, 377
MhlI GDGCHC 1 cut(s) 102
MluCI AATT 1 cut(s) 493
MlyI GAGTC 1 cut(s) 147
MnlI CCTC 8 cut(s) 143, 273, 279, 285, 304, 307, 313, 390
MspA1I CMGCKG 1 cut(s) 25
MspI CCGG 1 cut(s) 293
MwoI GCNNNNNNNGC 6 cut(s) 116, 240, 280, 454, 463, 472
NdeII GATC 2 cut(s) 123, 255
NlaIII CATG 3 cut(s) 187, 194, 219
NlaIV GGNNCC 1 cut(s) 100
NmeAIII GCCGAG 1 cut(s) 352
NmuCI GTSAC 2 cut(s) 52, 83
NspV TTCGAA 1 cut(s) 210
PaeR7I CTCGAG 1 cut(s) 221
PcsI WCGNNNNNNNCGW 1 cut(s) 255
PleI GAGTC 1 cut(s) 146
PpsI GAGTC 1 cut(s) 146
PspN4I GGNNCC 1 cut(s) 100
PspOMI GGGCCC 1 cut(s) 98
PspPI GGNCC 3 cut(s) 19, 98, 99
PvuII CAGCTG 1 cut(s) 25
RsaI GTAC 1 cut(s) 375
RsaNI GTAC 1 cut(s) 374
Sau3AI GATC 2 cut(s) 123, 255
Sau96I GGNCC 3 cut(s) 19, 98, 99
SchI GAGTC 1 cut(s) 147
SduI GDGCHC 1 cut(s) 102
SetI ASST 9 cut(s) 16, 27, 160, 175, 265, 318, 333, 382, 403
SfaNI GCATC 1 cut(s) 221
Sfr274I CTCGAG 1 cut(s) 221
SfuI TTCGAA 1 cut(s) 210
SinI GGWCC 1 cut(s) 19
SlaI CTCGAG 1 cut(s) 221
SmlI CTYRAG 1 cut(s) 221
SmoI CTYRAG 1 cut(s) 221
Sse9I AATT 1 cut(s) 493
SsiI CCGC 9 cut(s) 6, 110, 146, 266, 272, 278, 281, 300, 323
TaaI ACNGT 1 cut(s) 251
TaiI ACGT 1 cut(s) 403
TaqI TCGA 4 cut(s) 210, 222, 258, 309
TaqII GACCGA 1 cut(s) 82
TasI AATT 1 cut(s) 493
TatI WGTACW 1 cut(s) 373
TauI GCSGC 2 cut(s) 283, 326
TscAI CASTG 1 cut(s) 126
TseFI GTSAC 2 cut(s) 52, 83
Tsp45I GTSAC 2 cut(s) 52, 83
TspDTI ATGAA 1 cut(s) 371
TspGWI ACGGA 1 cut(s) 295
TspRI CASTG 1 cut(s) 126
VpaK11BI GGWCC 1 cut(s) 19
XapI RAATTY 1 cut(s) 493
XhoI CTCGAG 1 cut(s) 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.