Rmu_ssc0000451.1_g000001

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000451.1
OG0004388
Physical Location & Seq
Reverse (-)
593 .. 1743
1151 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 903 bp
atgctattcaacaatggtgtaggagggaatagtactaatcagcgcaagagaggaagagaaacttcagttggcactgatatcacaccttcaatgattcccttctctttgcaacagtcccaacctcctcaactcatcgacctctctcagctccacaaccacaaccaccatcccaatgtcgtctccaccggcctccgattatcttttggagaccaacaacaacaattgcaacagcatcatcacaatcaacaacaacaacaggattgtcactcttcttcatctctgttgtctgttttggcagtggactttgcctcccaaatcaaacaacagagggacgaattagaccagttccttcaagcccagggggaacaactacagcgcacattagcagagaagcggcagacacactaccgtgcgctactgggcgcagctgaggagtctatttcccgtaaattgcgagagaaggaggcagaggtccaaaaggccacgcgcagaaacgccgagttagaggcacgcgccgcccagctcagcgttgaggctcagatatggcaggccaaggcccgtgcgcaggaggccaccgccgcgtccctccaggtccaagtccaacaggccaaggccatgatgagcgcggggttccaagtggcgggtgcgcaggatagcaggagagccgacgatgggctaacatgcgccgaaaaccaggcggaggatgccgagtcagggtacattgacccggaacgcgccaccgggtcgggtccgagttgtaaagcgtgtcggaaaggagtggcgtcagtggtgctgttgccgtgtcggcacctatgcctctgtacagagtgtgaccaaatggttcagtcctgcccattgtgtctcaccttgagaaattcgagtgtggaagtcttcctttgctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prok-RING_4 zf-C3HC4_3

Protein Analysis

300

Amino Acids

33.06

Weight (kDa)

6.55

Isoelectric Point (pI)

60.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 252 - 288 2.3e-09 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 564, 648
AccB1I GGYRCC 1 cut(s) 807
AccII CGCG 5 cut(s) 487, 513, 581, 626, 735
AciI CCGC 7 cut(s) 394, 516, 576, 579, 626, 641, 698
AcsI RAATTY 1 cut(s) 874
AcuI CTGAAG 1 cut(s) 48
AcyI GRCGYC 1 cut(s) 782
AfaI GTAC 3 cut(s) 34, 719, 823
AfiI CCNNNNNNNGG 5 cut(s) 565, 640, 672, 700, 714
AgsI TTSAA 3 cut(s) 10, 90, 353
AjnI CCWGG 3 cut(s) 357, 588, 693
AjuI GAANNNNNNNTTGG 2 cut(s) 51, 83
AleI CACNNNNGTG 1 cut(s) 408
AluBI AGCT 3 cut(s) 148, 428, 523
AluI AGCT 3 cut(s) 148, 428, 523
Alw26I GTCTC 3 cut(s) 184, 201, 866
AoxI GGCC 7 cut(s) 187, 480, 549, 555, 570, 606, 612
ApeKI GCWGC 1 cut(s) 425
ApoI RAATTY 1 cut(s) 874
AspS9I GGNCC 4 cut(s) 472, 556, 592, 749
AsuC2I CCSGG 2 cut(s) 728, 742
AsuHPI GGTGA 1 cut(s) 856
AvaII GGWCC 3 cut(s) 472, 592, 749
BanI GGYRCC 1 cut(s) 807
BbsI GAAGAC 1 cut(s) 883
BbvCI CCTCAGC 1 cut(s) 429
BbvI GCAGC 1 cut(s) 437
BccI CCATC 2 cut(s) 174, 665
BceAI ACGGC 1 cut(s) 784
BciT130I CCWGG 3 cut(s) 359, 590, 695
BcnI CCSGG 2 cut(s) 728, 742
BcoDI GTCTC 3 cut(s) 184, 201, 866
BfaI CTAG 1 cut(s) 901
BfmI CTRYAG 1 cut(s) 371
BglI GCCNNNNNGGC 1 cut(s) 805
BisI GCNGC 4 cut(s) 395, 426, 516, 579
BlpI GCTNAGC 1 cut(s) 524
BlsI GCNGC 4 cut(s) 396, 427, 517, 580
BmcAI AGTACT 1 cut(s) 34
Bme1390I CCNGG 5 cut(s) 359, 590, 695, 728, 742
Bme18I GGWCC 3 cut(s) 472, 592, 749
BmgT120I GGNCC 4 cut(s) 472, 556, 592, 749
BmiI GGNNCC 3 cut(s) 632, 750, 809
BmrFI CCNGG 5 cut(s) 359, 590, 695, 728, 742
BmrI ACTGGG 1 cut(s) 428
BmsI GCATC 2 cut(s) 241, 694
BmuI ACTGGG 1 cut(s) 428
BpiI GAAGAC 1 cut(s) 883
BpmI CTGGAG 1 cut(s) 572
Bpu10I CCTNAGC 1 cut(s) 429
Bpu1102I GCTNAGC 1 cut(s) 524
BpuEI CTTGAG 1 cut(s) 889
BpuMI CCSGG 2 cut(s) 728, 742
BsaHI GRCGYC 1 cut(s) 782
BsaI GGTCTC 1 cut(s) 201
BsaJI CCNNGG 4 cut(s) 357, 358, 552, 609
BsaXI ACNNNNNCTCC 2 cut(s) 293, 323
Bsc4I CCNNNNNNNGG 5 cut(s) 565, 640, 672, 700, 714
Bse118I RCCGGY 1 cut(s) 185
Bse1I ACTGG 2 cut(s) 343, 423
BseBI CCWGG 3 cut(s) 359, 590, 695
BseDI CCNNGG 4 cut(s) 357, 358, 552, 609
BseGI GGATG 2 cut(s) 166, 709
BseLI CCNNNNNNNGG 5 cut(s) 565, 640, 672, 700, 714
BseMII CTCAG 4 cut(s) 158, 420, 538, 551
BseNI ACTGG 2 cut(s) 343, 423
BseRI GAGGAG 2 cut(s) 114, 446
BseXI GCAGC 1 cut(s) 437
BseYI CCCAGC 1 cut(s) 519
Bsh1236I CGCG 5 cut(s) 487, 513, 581, 626, 735
BshFI GGCC 7 cut(s) 189, 482, 551, 557, 572, 608, 614
BshNI GGYRCC 1 cut(s) 807
BsiSI CCGG 3 cut(s) 186, 728, 741
BslFI GGGAC 3 cut(s) 100, 344, 568
BslI CCNNNNNNNGG 5 cut(s) 565, 640, 672, 700, 714
BsmAI GTCTC 3 cut(s) 184, 201, 866
BsmBI CGTCTC 1 cut(s) 184
BsmFI GGGAC 3 cut(s) 100, 344, 568
BsnI GGCC 7 cut(s) 189, 482, 551, 557, 572, 608, 614
Bso31I GGTCTC 1 cut(s) 201
Bsp1407I TGTACA 1 cut(s) 821
Bsp1720I GCTNAGC 1 cut(s) 524
BspACI CCGC 7 cut(s) 394, 516, 576, 579, 626, 641, 698
BspANI GGCC 7 cut(s) 189, 482, 551, 557, 572, 608, 614
BspCNI CTCAG 4 cut(s) 157, 421, 537, 550
BspFNI CGCG 5 cut(s) 487, 513, 581, 626, 735
BspLI GGNNCC 3 cut(s) 632, 750, 809
BspT107I GGYRCC 1 cut(s) 807
BspTNI GGTCTC 1 cut(s) 201
BsrFI RCCGGY 1 cut(s) 185
BsrGI TGTACA 1 cut(s) 821
BsrI ACTGG 2 cut(s) 343, 423
BssAI RCCGGY 1 cut(s) 185
BssECI CCNNGG 4 cut(s) 357, 358, 552, 609
BssNI GRCGYC 1 cut(s) 782
BssT1I CCWWGG 2 cut(s) 552, 609
Bst2UI CCWGG 3 cut(s) 359, 590, 695
Bst4CI ACNGT 2 cut(s) 114, 410
Bst6I CTCTTC 2 cut(s) 49, 274
BstACI GRCGYC 1 cut(s) 782
BstAUI TGTACA 1 cut(s) 821
BstC8I GCNNGC 2 cut(s) 511, 549
BstDEI CTNAG 4 cut(s) 144, 429, 524, 537
BstF5I GGATG 2 cut(s) 166, 709
BstFNI CGCG 5 cut(s) 487, 513, 581, 626, 735
BstMAI GTCTC 3 cut(s) 184, 201, 866
BstMWI GCNNNNNNNGC 5 cut(s) 515, 569, 578, 704, 805
BstNI CCWGG 3 cut(s) 359, 590, 695
BstNSI RCATGY 1 cut(s) 684
BstSCI CCNGG 5 cut(s) 357, 588, 693, 726, 740
BstSFI CTRYAG 1 cut(s) 371
BstUI CGCG 5 cut(s) 487, 513, 581, 626, 735
BstV1I GCAGC 1 cut(s) 437
BstV2I GAAGAC 1 cut(s) 883
BsuRI GGCC 7 cut(s) 189, 482, 551, 557, 572, 608, 614
BtsCI GGATG 2 cut(s) 166, 709
BtsI GCAGTG 1 cut(s) 303
BtsIMutI CAGTG 3 cut(s) 72, 303, 792
Cac8I GCNNGC 2 cut(s) 511, 549
Cfr10I RCCGGY 1 cut(s) 185
Cfr13I GGNCC 4 cut(s) 472, 556, 592, 749
CseI GACGC 2 cut(s) 570, 771
Csp6I GTAC 3 cut(s) 33, 718, 822
CviAII CATG 2 cut(s) 616, 681
CviQI GTAC 3 cut(s) 33, 718, 822
DdeI CTNAG 4 cut(s) 144, 429, 524, 537
Eam1104I CTCTTC 2 cut(s) 49, 274
EarI CTCTTC 2 cut(s) 49, 274
EciI GGCGGA 1 cut(s) 713
Eco130I CCWWGG 2 cut(s) 552, 609
Eco31I GGTCTC 1 cut(s) 201
Eco32I GATATC 1 cut(s) 79
Eco47I GGWCC 3 cut(s) 472, 592, 749
Eco57I CTGAAG 1 cut(s) 48
EcoRII CCWGG 3 cut(s) 357, 588, 693
EcoRV GATATC 1 cut(s) 79
EcoT14I CCWWGG 2 cut(s) 552, 609
ErhI CCWWGG 2 cut(s) 552, 609
Esp3I CGTCTC 1 cut(s) 184
FaeI CATG 2 cut(s) 619, 684
FaiI YATR 4 cut(s) 544, 617, 682, 814
FalI AAGNNNNNCTT 3 cut(s) 46, 78, 879
FaqI GGGAC 3 cut(s) 100, 344, 568
FatI CATG 2 cut(s) 615, 680
FauI CCCGC 2 cut(s) 619, 634
Fnu4HI GCNGC 4 cut(s) 395, 426, 516, 579
FokI GGATG 2 cut(s) 153, 716
Fsp4HI GCNGC 4 cut(s) 395, 426, 516, 579
FspBI CTAG 1 cut(s) 901
FspI TGCGCA 2 cut(s) 564, 648
GluI GCNGC 4 cut(s) 395, 426, 516, 579
GsaI CCCAGC 1 cut(s) 523
GsuI CTGGAG 1 cut(s) 572
HaeIII GGCC 7 cut(s) 189, 482, 551, 557, 572, 608, 614
HapII CCGG 3 cut(s) 186, 728, 741
HgaI GACGC 2 cut(s) 570, 771
Hin1I GRCGYC 1 cut(s) 782
Hin1II CATG 2 cut(s) 619, 684
HinfI GANTC 3 cut(s) 94, 434, 710
HpaII CCGG 3 cut(s) 186, 728, 741
HphI GGTGA 1 cut(s) 856
Hpy166II GTNNAC 1 cut(s) 301
Hpy188I TCNGA 4 cut(s) 194, 540, 753, 771
Hpy8I GTNNAC 1 cut(s) 301
Hpy99I CGWCG 1 cut(s) 671
HpyAV CCTTC 4 cut(s) 96, 109, 359, 454
HpyCH4III ACNGT 2 cut(s) 114, 410
HpyCH4V TGCA 2 cut(s) 109, 226
HpyF10VI GCNNNNNNNGC 5 cut(s) 515, 569, 578, 704, 805
HpyF3I CTNAG 4 cut(s) 144, 429, 524, 537
Hsp92I GRCGYC 1 cut(s) 782
Hsp92II CATG 2 cut(s) 619, 684
LmnI GCTCC 1 cut(s) 153
Lsp1109I GCAGC 1 cut(s) 437
LweI GCATC 2 cut(s) 241, 694
MaeI CTAG 1 cut(s) 901
MaeIII GTNAC 2 cut(s) 263, 830
MboII GAAGA 4 cut(s) 66, 261, 264, 883
MfeI CAATTG 1 cut(s) 221
MluCI AATT 4 cut(s) 221, 335, 449, 874
MlyI GAGTC 2 cut(s) 443, 719
MmeI TCCRAC 2 cut(s) 625, 749
MslI CAYNNNNRTG 2 cut(s) 171, 408
MspA1I CMGCKG 1 cut(s) 428
MspI CCGG 3 cut(s) 186, 728, 741
MspR9I CCNGG 5 cut(s) 359, 590, 695, 728, 742
MunI CAATTG 1 cut(s) 221
MvaI CCWGG 3 cut(s) 359, 590, 695
MvnI CGCG 5 cut(s) 487, 513, 581, 626, 735
MwoI GCNNNNNNNGC 5 cut(s) 515, 569, 578, 704, 805
NciI CCSGG 2 cut(s) 728, 742
NlaIII CATG 2 cut(s) 619, 684
NlaIV GGNNCC 3 cut(s) 632, 750, 809
NmeAIII GCCGAG 2 cut(s) 523, 733
NmuCI GTSAC 2 cut(s) 263, 830
NsbI TGCGCA 2 cut(s) 564, 648
NspI RCATGY 1 cut(s) 684
OliI CACNNNNGTG 1 cut(s) 408
PasI CCCWGGG 1 cut(s) 358
PfeI GAWTC 1 cut(s) 94
PkrI GCNGC 4 cut(s) 396, 427, 517, 580
PleI GAGTC 2 cut(s) 442, 718
PpsI GAGTC 2 cut(s) 442, 718
Psp6I CCWGG 3 cut(s) 357, 588, 693
PspFI CCCAGC 1 cut(s) 519
PspGI CCWGG 3 cut(s) 357, 588, 693
PspN4I GGNNCC 3 cut(s) 632, 750, 809
PspPI GGNCC 4 cut(s) 472, 556, 592, 749
PvuII CAGCTG 1 cut(s) 428
RsaI GTAC 3 cut(s) 34, 719, 823
RsaNI GTAC 3 cut(s) 33, 718, 822
RseI CAYNNNNRTG 2 cut(s) 171, 408
SatI GCNGC 4 cut(s) 395, 426, 516, 579
Sau96I GGNCC 4 cut(s) 472, 556, 592, 749
ScaI AGTACT 1 cut(s) 34
SchI GAGTC 2 cut(s) 443, 719
ScrFI CCNGG 5 cut(s) 359, 590, 695, 728, 742
SfaNI GCATC 2 cut(s) 241, 694
SfcI CTRYAG 1 cut(s) 371
SinI GGWCC 3 cut(s) 472, 592, 749
SmiMI CAYNNNNRTG 2 cut(s) 171, 408
SmlI CTYRAG 1 cut(s) 868
SmoI CTYRAG 1 cut(s) 868
Sse9I AATT 4 cut(s) 221, 335, 449, 874
SsiI CCGC 7 cut(s) 394, 516, 576, 579, 626, 641, 698
SspMI CTAG 1 cut(s) 901
StyD4I CCNGG 5 cut(s) 357, 588, 693, 726, 740
StyI CCWWGG 2 cut(s) 552, 609
TaaI ACNGT 2 cut(s) 114, 410
TaqI TCGA 2 cut(s) 135, 878
TasI AATT 4 cut(s) 221, 335, 449, 874
TatI WGTACW 2 cut(s) 32, 821
TauI GCSGC 3 cut(s) 397, 518, 581
TfiI GAWTC 1 cut(s) 94
TscAI CASTG 3 cut(s) 79, 303, 792
TseFI GTSAC 2 cut(s) 263, 830
TseI GCWGC 1 cut(s) 425
Tsp45I GTSAC 2 cut(s) 263, 830
TspDTI ATGAA 1 cut(s) 264
TspRI CASTG 3 cut(s) 79, 303, 792
VpaK11BI GGWCC 3 cut(s) 472, 592, 749
XapI RAATTY 1 cut(s) 874
XceI RCATGY 1 cut(s) 684
XspI CTAG 1 cut(s) 901
ZrmI AGTACT 1 cut(s) 34
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.