Rroxscaffold_2G00085420

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0007546
Physical Location & Seq
Forward (+)
7748535 .. 7753682
5148 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00085420.1

Sequence Viewer

Length: 717 bp
ATGAATATCTTCGTAGAAAGGTACTCTCTTTTTCTATTTATCAAATTATTTAAAGCTGTGTTTCACATCGACCGATATTTCTATTTGTTGGTGATGATGTATACTATTCGGACAAGCGTATTAAGGAGTAACTTGAATCCGGTCTTGAATGAGGAGCTCATGTTTTCTGTTCCTTGGCGATATGGAGAATTGAAGGCATGGAGCTTTACGGTTGAGTTACCGCAGCAAATAAACAAACACGTGGCATCGCGCGGGATGCTCGTTAGTGTCTTATTAAACTGCCTTTCTGTTTCAATGTGCATAGGCCTGCACTGGGTCGAGTTACATCATATGATGCTTGAAGATTTGCCGAATGGTATTATGGATGAGGACCAAAAGATTCACCAAAAGATATATCTAGGAAAGATAGCTAGAAGTCTCGTCGATCTGAGCTACCAGCCACAAGTGATGAGTTCATGTTACTGTGGCGTTCTCAGATTCATAGATACATCACAATTAGTTGTACGGCCAAACACATTTGTTAAGCTACATGATCTCACTTCTAAAGCCCTATGGTTTTTAAATAGTGTCATGATCATTTTGCCCCTTCTCATGTTTTGGCTTTGTTTCACGCTTCTTCCTTTTTGGGTTTTGGTTGTTAATTTGCTCGACAACGAGAGTGAAGAGAGCGAGAGGAAGAAGGGAGAGAAGAAGAGGGAGACGGGAAGCTCTCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

238

Amino Acids

27.95

Weight (kDa)

8.55

Isoelectric Point (pI)

40.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 101
AccII CGCG 2 cut(s) 250, 252
AciI CCGC 2 cut(s) 221, 252
AcoI YGGCCR 1 cut(s) 506
AcvI CACGTG 1 cut(s) 241
AfaI GTAC 2 cut(s) 23, 504
AfiI CCNNNNNNNGG 1 cut(s) 313
AflIII ACRYGT 1 cut(s) 238
AgsI TTSAA 5 cut(s) 136, 148, 193, 294, 341
AluBI AGCT 7 cut(s) 56, 157, 204, 410, 432, 526, 708
AluI AGCT 7 cut(s) 56, 157, 204, 410, 432, 526, 708
Alw21I GWGCWC 1 cut(s) 159
Alw26I GTCTC 2 cut(s) 422, 692
AoxI GGCC 2 cut(s) 304, 506
ApeKI GCWGC 1 cut(s) 223
Asp700I GAANNNNTTC 1 cut(s) 8
AspLEI GCGC 1 cut(s) 252
AspS9I GGNCC 1 cut(s) 370
AsuHPI GGTGA 2 cut(s) 103, 374
AvaII GGWCC 1 cut(s) 370
BanII GRGCYC 1 cut(s) 159
BbrPI CACGTG 1 cut(s) 241
Bbv12I GWGCWC 1 cut(s) 159
BbvI GCAGC 1 cut(s) 235
BceAI ACGGC 1 cut(s) 521
BclI TGATCA 1 cut(s) 573
BcoDI GTCTC 2 cut(s) 422, 692
BfaI CTAG 2 cut(s) 398, 411
BisI GCNGC 1 cut(s) 224
BlsI GCNGC 1 cut(s) 225
Bme18I GGWCC 1 cut(s) 370
BmgT120I GGNCC 1 cut(s) 370
BmrI ACTGGG 1 cut(s) 322
BmsI GCATC 3 cut(s) 246, 254, 324
BmuI ACTGGG 1 cut(s) 322
BsaAI YACGTR 1 cut(s) 241
BsaJI CCNNGG 1 cut(s) 173
BsaWI WCCGGW 1 cut(s) 139
BsaXI ACNNNNNCTCC 2 cut(s) 146, 176
Bsc4I CCNNNNNNNGG 1 cut(s) 313
Bse1I ACTGG 1 cut(s) 317
BseDI CCNNGG 1 cut(s) 173
BseGI GGATG 2 cut(s) 261, 370
BseLI CCNNNNNNNGG 1 cut(s) 313
BseMII CTCAG 2 cut(s) 419, 487
BseNI ACTGG 1 cut(s) 317
BseRI GAGGAG 1 cut(s) 167
BseXI GCAGC 1 cut(s) 235
BsgI GTGCAG 1 cut(s) 293
Bsh1236I CGCG 2 cut(s) 250, 252
Bsh1285I CGRYCG 1 cut(s) 73
BshFI GGCC 2 cut(s) 306, 508
BsiEI CGRYCG 1 cut(s) 73
BsiHKAI GWGCWC 1 cut(s) 159
BsiSI CCGG 1 cut(s) 140
BslI CCNNNNNNNGG 1 cut(s) 313
BsmAI GTCTC 2 cut(s) 422, 692
BsmBI CGTCTC 1 cut(s) 692
BsnI GGCC 2 cut(s) 306, 508
Bsp1286I GDGCHC 1 cut(s) 159
Bsp143I GATC 3 cut(s) 424, 532, 573
BspACI CCGC 2 cut(s) 221, 252
BspANI GGCC 2 cut(s) 306, 508
BspCNI CTCAG 2 cut(s) 420, 486
BspFNI CGCG 2 cut(s) 250, 252
BspHI TCATGA 1 cut(s) 570
BsrI ACTGG 1 cut(s) 317
BssECI CCNNGG 1 cut(s) 173
BssMI GATC 3 cut(s) 424, 532, 573
BssNAI GTATAC 1 cut(s) 102
BssT1I CCWWGG 1 cut(s) 173
Bst1107I GTATAC 1 cut(s) 102
Bst4CI ACNGT 2 cut(s) 211, 464
Bst6I CTCTTC 2 cut(s) 657, 686
BstBAI YACGTR 1 cut(s) 241
BstC8I GCNNGC 1 cut(s) 308
BstDEI CTNAG 2 cut(s) 428, 473
BstF5I GGATG 2 cut(s) 261, 370
BstFNI CGCG 2 cut(s) 250, 252
BstHHI GCGC 1 cut(s) 252
BstKTI GATC 3 cut(s) 427, 535, 576
BstMAI GTCTC 2 cut(s) 422, 692
BstMBI GATC 3 cut(s) 424, 532, 573
BstMCI CGRYCG 1 cut(s) 73
BstMWI GCNNNNNNNGC 1 cut(s) 256
BstUI CGCG 2 cut(s) 250, 252
BstV1I GCAGC 1 cut(s) 235
BstZ17I GTATAC 1 cut(s) 102
BsuRI GGCC 2 cut(s) 306, 508
BtgZI GCGATG 1 cut(s) 231
BtsCI GGATG 2 cut(s) 261, 370
BtsIMutI CAGTG 1 cut(s) 310
Cac8I GCNNGC 1 cut(s) 308
CciI TCATGA 1 cut(s) 570
CfoI GCGC 1 cut(s) 252
Cfr13I GGNCC 1 cut(s) 370
Csp6I GTAC 2 cut(s) 22, 503
CviAII CATG 6 cut(s) 160, 198, 456, 530, 571, 592
CviQI GTAC 2 cut(s) 22, 503
DdeI CTNAG 2 cut(s) 428, 473
DpnI GATC 3 cut(s) 426, 534, 575
DpnII GATC 3 cut(s) 424, 532, 573
DraI TTTAAA 2 cut(s) 52, 561
EaeI YGGCCR 1 cut(s) 506
Eam1104I CTCTTC 2 cut(s) 657, 686
EarI CTCTTC 2 cut(s) 657, 686
Ecl136II GAGCTC 1 cut(s) 157
Eco130I CCWWGG 1 cut(s) 173
Eco147I AGGCCT 1 cut(s) 306
Eco24I GRGCYC 1 cut(s) 159
Eco47I GGWCC 1 cut(s) 370
Eco53kI GAGCTC 1 cut(s) 157
Eco72I CACGTG 1 cut(s) 241
EcoICRI GAGCTC 1 cut(s) 157
EcoT14I CCWWGG 1 cut(s) 173
EcoT38I GRGCYC 1 cut(s) 159
ErhI CCWWGG 1 cut(s) 173
Esp3I CGTCTC 1 cut(s) 692
FaeI CATG 6 cut(s) 163, 201, 459, 533, 574, 595
FatI CATG 6 cut(s) 159, 197, 455, 529, 570, 591
FauI CCCGC 1 cut(s) 245
FauNDI CATATG 1 cut(s) 330
FbaI TGATCA 1 cut(s) 573
FblI GTMKAC 1 cut(s) 101
Fnu4HI GCNGC 1 cut(s) 224
FokI GGATG 2 cut(s) 268, 377
FriOI GRGCYC 1 cut(s) 159
Fsp4HI GCNGC 1 cut(s) 224
FspBI CTAG 2 cut(s) 398, 411
GlaI GCGC 1 cut(s) 251
GluI GCNGC 1 cut(s) 224
HaeIII GGCC 2 cut(s) 306, 508
HapII CCGG 1 cut(s) 140
HhaI GCGC 1 cut(s) 252
Hin1II CATG 6 cut(s) 163, 201, 459, 533, 574, 595
Hin6I GCGC 1 cut(s) 250
HinP1I GCGC 1 cut(s) 250
HinfI GANTC 3 cut(s) 136, 379, 477
HpaII CCGG 1 cut(s) 140
HphI GGTGA 2 cut(s) 103, 374
Hpy166II GTNNAC 1 cut(s) 102
Hpy188I TCNGA 3 cut(s) 111, 429, 476
Hpy188III TCNNGA 2 cut(s) 145, 571
Hpy8I GTNNAC 1 cut(s) 102
Hpy99I CGWCG 1 cut(s) 425
HpyAV CCTTC 3 cut(s) 187, 596, 673
HpyCH4III ACNGT 2 cut(s) 211, 464
HpyCH4IV ACGT 1 cut(s) 240
HpyCH4V TGCA 2 cut(s) 300, 310
HpyF10VI GCNNNNNNNGC 1 cut(s) 256
HpyF3I CTNAG 2 cut(s) 428, 473
HpySE526I ACGT 1 cut(s) 240
Hsp92II CATG 6 cut(s) 163, 201, 459, 533, 574, 595
HspAI GCGC 1 cut(s) 250
Ksp22I TGATCA 1 cut(s) 573
Kzo9I GATC 3 cut(s) 424, 532, 573
LmnI GCTCC 2 cut(s) 154, 201
LpnPI CCDG 4 cut(s) 153, 298, 320, 449
Lsp1109I GCAGC 1 cut(s) 235
LweI GCATC 3 cut(s) 246, 254, 324
MaeI CTAG 2 cut(s) 398, 411
MaeII ACGT 1 cut(s) 240
MaeIII GTNAC 4 cut(s) 128, 216, 321, 458
MalI GATC 3 cut(s) 426, 534, 575
MboI GATC 3 cut(s) 424, 532, 573
MboII GAAGA 6 cut(s) 353, 608, 674, 688, 700, 703
MhlI GDGCHC 1 cut(s) 159
MluCI AATT 4 cut(s) 44, 188, 494, 640
MnlI CCTC 4 cut(s) 145, 361, 666, 687
MroXI GAANNNNTTC 1 cut(s) 8
MseI TTAA 6 cut(s) 51, 122, 275, 522, 560, 639
MspI CCGG 1 cut(s) 140
MvnI CGCG 2 cut(s) 250, 252
MwoI GCNNNNNNNGC 1 cut(s) 256
NdeI CATATG 1 cut(s) 330
NdeII GATC 3 cut(s) 424, 532, 573
NlaIII CATG 6 cut(s) 163, 201, 459, 533, 574, 595
PagI TCATGA 1 cut(s) 570
PceI AGGCCT 1 cut(s) 306
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 3 cut(s) 136, 379, 477
PkrI GCNGC 1 cut(s) 225
PmaCI CACGTG 1 cut(s) 241
PmlI CACGTG 1 cut(s) 241
Ppu21I YACGTR 1 cut(s) 241
Psp124BI GAGCTC 1 cut(s) 159
PspCI CACGTG 1 cut(s) 241
PspPI GGNCC 1 cut(s) 370
RsaI GTAC 2 cut(s) 23, 504
RsaNI GTAC 2 cut(s) 22, 503
SacI GAGCTC 1 cut(s) 159
SaqAI TTAA 6 cut(s) 51, 122, 275, 522, 560, 639
SatI GCNGC 1 cut(s) 224
Sau3AI GATC 3 cut(s) 424, 532, 573
Sau96I GGNCC 1 cut(s) 370
SduI GDGCHC 1 cut(s) 159
SetI ASST 9 cut(s) 23, 58, 159, 206, 243, 412, 434, 528, 710
SfaNI GCATC 3 cut(s) 246, 254, 324
SinI GGWCC 1 cut(s) 370
Sse9I AATT 4 cut(s) 44, 188, 494, 640
SseBI AGGCCT 1 cut(s) 306
SsiI CCGC 2 cut(s) 221, 252
SspMI CTAG 2 cut(s) 398, 411
SstI GAGCTC 1 cut(s) 159
StuI AGGCCT 1 cut(s) 306
StyI CCWWGG 1 cut(s) 173
TaaI ACNGT 2 cut(s) 211, 464
TaiI ACGT 1 cut(s) 243
TaqI TCGA 4 cut(s) 69, 318, 423, 648
TaqII GACCGA 1 cut(s) 87
TasI AATT 4 cut(s) 44, 188, 494, 640
TfiI GAWTC 3 cut(s) 136, 379, 477
Tru1I TTAA 6 cut(s) 51, 122, 275, 522, 560, 639
Tru9I TTAA 6 cut(s) 51, 122, 275, 522, 560, 639
TscAI CASTG 1 cut(s) 317
TseI GCWGC 1 cut(s) 223
TspDTI ATGAA 3 cut(s) 17, 444, 469
TspRI CASTG 1 cut(s) 317
VpaK11BI GGWCC 1 cut(s) 370
XmiI GTMKAC 1 cut(s) 101
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 2 cut(s) 398, 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.