Rroxscaffold_2G00111370

Belongs to the eukaryotic ribosomal protein eS12 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002614
Physical Location & Seq
Forward (+)
35966867 .. 35968489
1623 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00111370.1

Sequence Viewer

Length: 450 bp
ATGTCTGGGGTTTTGGAGATGAATATCATTAGTGATGAAGCACCCGCACCAGTTGTAGCTGAAGCACCTGCTCCAGCCCTTGGCGAGCCTATGGATATCATGACAGCCGTGCAGCTTGTGCTGAGAAAATCGCTGGCTCATGGTGGGCTTGTTCGAGGTCTTCATGAAGCTGCAAAGGTTGTTGAGAAGCATGCTGCCCAGCTCTGTGTCCTGGCTGAGGACTGTGACCAGCAAGACTATGTTAAACTAGTCAAAGGTCTTTGTGCTGAGCACAATGTAAACATGTTGACTGTTCCCAATGCCAAGACCCTAGGAGAGTGGGCTGGTTTGTGCAAGATCGACTCCGAGGGCAAGGCTAGGAAGGTTGTTGGTTGCTCATGTGTTGTTGTGAAGGATTTTGGAGAGGATCATGAAGCTCTCCATGTTGTTCAGCAGCATGTCAAGGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L7Ae

Protein Analysis

149

Amino Acids

15.85

Weight (kDa)

5.59

Isoelectric Point (pI)

32.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L7Ae PF01248 36 - 129 1.4e-26 Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 76
Acc36I ACCTGC 1 cut(s) 76
AccB7I CCANNNNNTGG 1 cut(s) 80
AciI CCGC 1 cut(s) 45
AclWI GGATC 1 cut(s) 414
AcuI CTGAAG 1 cut(s) 81
AfiI CCNNNNNNNGG 2 cut(s) 80, 217
AflIII ACRYGT 1 cut(s) 282
AhlI ACTAGT 1 cut(s) 247
AjnI CCWGG 1 cut(s) 210
AluBI AGCT 5 cut(s) 59, 115, 170, 202, 416
AluI AGCT 5 cut(s) 59, 115, 170, 202, 416
Alw21I GWGCWC 1 cut(s) 273
AlwI GGATC 1 cut(s) 414
AoxI GGCC 1 cut(s) 444
ApeKI GCWGC 4 cut(s) 112, 170, 194, 433
AspA2I CCTAGG 1 cut(s) 310
AvrII CCTAGG 1 cut(s) 310
BbsI GAAGAC 1 cut(s) 152
Bbv12I GWGCWC 1 cut(s) 273
BbvCI CCTCAGC 1 cut(s) 216
BbvI GCAGC 4 cut(s) 124, 157, 181, 445
BceAI ACGGC 1 cut(s) 92
BciT130I CCWGG 1 cut(s) 212
BcuI ACTAGT 1 cut(s) 247
BfaI CTAG 3 cut(s) 248, 311, 357
BfuAI ACCTGC 1 cut(s) 76
BisI GCNGC 4 cut(s) 113, 171, 195, 434
BlnI CCTAGG 1 cut(s) 310
BlpI GCTNAGC 1 cut(s) 267
BlsI GCNGC 4 cut(s) 114, 172, 196, 435
Bme1390I CCNGG 1 cut(s) 212
BmrFI CCNGG 1 cut(s) 212
BpiI GAAGAC 1 cut(s) 152
BpmI CTGGAG 1 cut(s) 57
Bpu10I CCTNAGC 1 cut(s) 216
Bpu1102I GCTNAGC 1 cut(s) 267
BsaBI GATNNNNATC 1 cut(s) 23
BsaJI CCNNGG 3 cut(s) 79, 310, 345
Bsc4I CCNNNNNNNGG 2 cut(s) 80, 217
Bse1I ACTGG 1 cut(s) 50
Bse8I GATNNNNATC 1 cut(s) 23
BseBI CCWGG 1 cut(s) 212
BseDI CCNNGG 3 cut(s) 79, 310, 345
BseJI GATNNNNATC 1 cut(s) 23
BseLI CCNNNNNNNGG 2 cut(s) 80, 217
BseMII CTCAG 3 cut(s) 113, 207, 258
BseNI ACTGG 1 cut(s) 50
BseXI GCAGC 4 cut(s) 124, 157, 181, 445
BseYI CCCAGC 1 cut(s) 198
BsgI GTGCAG 1 cut(s) 131
BshFI GGCC 1 cut(s) 446
BsiHKAI GWGCWC 1 cut(s) 273
BslI CCNNNNNNNGG 2 cut(s) 80, 217
BsnI GGCC 1 cut(s) 446
Bsp1286I GDGCHC 1 cut(s) 273
Bsp143I GATC 2 cut(s) 336, 406
Bsp1720I GCTNAGC 1 cut(s) 267
BspACI CCGC 1 cut(s) 45
BspANI GGCC 1 cut(s) 446
BspCNI CTCAG 3 cut(s) 114, 208, 259
BspHI TCATGA 3 cut(s) 99, 163, 409
BspMI ACCTGC 1 cut(s) 76
BspPI GGATC 1 cut(s) 414
BsrI ACTGG 1 cut(s) 50
BssECI CCNNGG 3 cut(s) 79, 310, 345
BssMI GATC 2 cut(s) 336, 406
BssT1I CCWWGG 2 cut(s) 79, 310
Bst2UI CCWGG 1 cut(s) 212
Bst4CI ACNGT 2 cut(s) 224, 292
BstAPI GCANNNNNTGC 1 cut(s) 118
BstC8I GCNNGC 3 cut(s) 86, 135, 192
BstDEI CTNAG 3 cut(s) 122, 216, 267
BstENI CCTNNNNNAGG 1 cut(s) 215
BstKTI GATC 2 cut(s) 339, 409
BstMBI GATC 2 cut(s) 336, 406
BstMWI GCNNNNNNNGC 1 cut(s) 118
BstNI CCWGG 1 cut(s) 212
BstNSI RCATGY 3 cut(s) 194, 286, 440
BstSCI CCNGG 1 cut(s) 210
BstV1I GCAGC 4 cut(s) 124, 157, 181, 445
BstV2I GAAGAC 1 cut(s) 152
BsuRI GGCC 1 cut(s) 446
BveI ACCTGC 1 cut(s) 76
Cac8I GCNNGC 3 cut(s) 86, 135, 192
CciI TCATGA 3 cut(s) 99, 163, 409
CviAII CATG 9 cut(s) 100, 140, 164, 191, 283, 378, 410, 422, 437
DdeI CTNAG 3 cut(s) 122, 216, 267
DpnI GATC 2 cut(s) 338, 408
DpnII GATC 2 cut(s) 336, 406
Eco130I CCWWGG 2 cut(s) 79, 310
Eco147I AGGCCT 1 cut(s) 446
Eco32I GATATC 1 cut(s) 97
Eco57I CTGAAG 1 cut(s) 81
EcoNI CCTNNNNNAGG 1 cut(s) 215
EcoRII CCWGG 1 cut(s) 210
EcoRV GATATC 1 cut(s) 97
EcoT14I CCWWGG 2 cut(s) 79, 310
ErhI CCWWGG 2 cut(s) 79, 310
FaeI CATG 9 cut(s) 103, 143, 167, 194, 286, 381, 413, 425, 440
FatI CATG 9 cut(s) 99, 139, 163, 190, 282, 377, 409, 421, 436
FauI CCCGC 1 cut(s) 52
Fnu4HI GCNGC 4 cut(s) 113, 171, 195, 434
Fsp4HI GCNGC 4 cut(s) 113, 171, 195, 434
FspBI CTAG 3 cut(s) 248, 311, 357
GluI GCNGC 4 cut(s) 113, 171, 195, 434
GsaI CCCAGC 1 cut(s) 202
GsuI CTGGAG 1 cut(s) 57
HaeIII GGCC 1 cut(s) 446
Hin1II CATG 9 cut(s) 103, 143, 167, 194, 286, 381, 413, 425, 440
HincII GTYRAC 1 cut(s) 288
HindII GTYRAC 1 cut(s) 288
HinfI GANTC 1 cut(s) 341
Hpy166II GTNNAC 2 cut(s) 280, 288
Hpy188I TCNGA 1 cut(s) 346
Hpy188III TCNNGA 3 cut(s) 100, 164, 410
Hpy8I GTNNAC 2 cut(s) 280, 288
HpyAV CCTTC 2 cut(s) 355, 385
HpyCH4III ACNGT 2 cut(s) 224, 292
HpyCH4V TGCA 3 cut(s) 112, 173, 333
HpyF10VI GCNNNNNNNGC 1 cut(s) 118
HpyF3I CTNAG 3 cut(s) 122, 216, 267
Hsp92II CATG 9 cut(s) 103, 143, 167, 194, 286, 381, 413, 425, 440
Kzo9I GATC 2 cut(s) 336, 406
LmnI GCTCC 1 cut(s) 76
LpnPI CCDG 9 cut(s) 63, 81, 87, 119, 197, 212, 224, 242, 309
Lsp1109I GCAGC 4 cut(s) 124, 157, 181, 445
MaeI CTAG 3 cut(s) 248, 311, 357
MaeIII GTNAC 1 cut(s) 224
MalI GATC 2 cut(s) 338, 408
MboI GATC 2 cut(s) 336, 406
MboII GAAGA 1 cut(s) 152
MhlI GDGCHC 1 cut(s) 273
MlyI GAGTC 1 cut(s) 335
MnlI CCTC 4 cut(s) 149, 211, 340, 397
MseI TTAA 1 cut(s) 243
MspR9I CCNGG 1 cut(s) 212
MvaI CCWGG 1 cut(s) 212
MwoI GCNNNNNNNGC 1 cut(s) 118
NdeII GATC 2 cut(s) 336, 406
NlaIII CATG 9 cut(s) 103, 143, 167, 194, 286, 381, 413, 425, 440
NmuCI GTSAC 1 cut(s) 224
NspI RCATGY 3 cut(s) 194, 286, 440
PaeI GCATGC 1 cut(s) 194
PagI TCATGA 3 cut(s) 99, 163, 409
PaqCI CACCTGC 1 cut(s) 76
PceI AGGCCT 1 cut(s) 446
PciI ACATGT 1 cut(s) 282
PflMI CCANNNNNTGG 1 cut(s) 80
PkrI GCNGC 4 cut(s) 114, 172, 196, 435
PleI GAGTC 1 cut(s) 335
PpsI GAGTC 1 cut(s) 335
PscI ACATGT 1 cut(s) 282
Psp6I CCWGG 1 cut(s) 210
PspFI CCCAGC 1 cut(s) 198
PspGI CCWGG 1 cut(s) 210
SaqAI TTAA 1 cut(s) 243
SatI GCNGC 4 cut(s) 113, 171, 195, 434
Sau3AI GATC 2 cut(s) 336, 406
SchI GAGTC 1 cut(s) 335
ScrFI CCNGG 1 cut(s) 212
SduI GDGCHC 1 cut(s) 273
SpeI ACTAGT 1 cut(s) 247
SphI GCATGC 1 cut(s) 194
SseBI AGGCCT 1 cut(s) 446
SsiI CCGC 1 cut(s) 45
SspMI CTAG 3 cut(s) 248, 311, 357
StuI AGGCCT 1 cut(s) 446
StyD4I CCNGG 1 cut(s) 210
StyI CCWWGG 2 cut(s) 79, 310
TaaI ACNGT 2 cut(s) 224, 292
TaqI TCGA 2 cut(s) 154, 339
Tru1I TTAA 1 cut(s) 243
Tru9I TTAA 1 cut(s) 243
TseFI GTSAC 1 cut(s) 224
TseI GCWGC 4 cut(s) 112, 170, 194, 433
Tsp45I GTSAC 1 cut(s) 224
TspDTI ATGAA 5 cut(s) 35, 51, 152, 180, 426
Van91I CCANNNNNTGG 1 cut(s) 80
XagI CCTNNNNNAGG 1 cut(s) 215
XceI RCATGY 3 cut(s) 194, 286, 440
XmaJI CCTAGG 1 cut(s) 310
XspI CTAG 3 cut(s) 248, 311, 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.