Rroxscaffold_4G00307350

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0006965
Physical Location & Seq
Reverse (-)
28601127 .. 28610320
9194 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00307350.1

Sequence Viewer

Length: 990 bp
ATGAGAGACTTGAGATTGGAGATCGGGTATCTCAACCGTGAAATGGTGTTCGGCAGGGGGAAATCGTGTCGATTGGCATTATTCGGAATGATGGCATCGACGTCCAGTTCCGGTCGAAGTCAAACCATCATATCTACGTGGAAGTATGAGAGGGTGATGACTCGGGATTGCATGAAGGATGATTGGTTTCTACGGCACCCTATGACGAACCCGAACCGATTTCACCCCGAAACCGAATCGAGCTGTGGGGCCCACCTTAGGAAAAGTTTTACAAAAAATTTGGTAGAACTTCCCCTAAAATTGACTACCCAAATTATACAAAACCTGGATATGAAACTTAAACTAATCTCGAACCATATGTACATTCCGGGTATAATTTACATTCCGAGAGTCCACTCACCGGTAAGCCTCGGAGCTAAGCCTCGCGCCTACGGGATCCTTTTCCCTCGAGGGCTCAATATGTCCTGTACAAATTTGGCAGTGCAATTAGATTTGCCTTTTAAGGGAGTAAATTTTGAAAGAGGCGAGAAGGAAAATGTGAAATCCTTTAGACAAAAGGGAAATGTTGTAACTAAAGGGGAAAAAAATAGAGAGAAGTTAATGGGAAAGAGAGGCAAGAATTGCATTCGGGGGAGTCGAACCAAGGGAGCCGCGCCGAGCAGCAGGTGTTGGGTAGCGAACCCAATCTTTGATACCCAAGTGGTATCAATATGCTCGATCGTGGTCGTCGAAGACCGTGTTGACGTGTCCCCCTACCGTCCCGGAGGATCATTTTTTACCGTAACGCCACATGGCGGTCGTTGTCAAAGTGAGGCGTTGCCTTGGGAATCACCGTTGGCGGAAGTCCCTTTGCCGATATCAAGTGATCAACATTGTCGTCGGAACACGCTCGTCGGCCTTGACCGGTGTATGGGTTCCTTTGCGATGTATAGGGAGAAGTTCCGCCAGCGAGTTGTGTTTAGCGGAAACTGTCTCACTTGCAAGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

329

Amino Acids

37.26

Weight (kDa)

9.89

Isoelectric Point (pI)

33.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 654
AatII GACGTC 1 cut(s) 104
AbsI CCTCGAGG 1 cut(s) 447
Acc36I ACCTGC 1 cut(s) 654
AccB1I GGYRCC 1 cut(s) 195
AccII CGCG 2 cut(s) 426, 653
AciI CCGC 5 cut(s) 651, 795, 839, 943, 963
AclWI GGATC 3 cut(s) 430, 443, 775
AcsI RAATTY 3 cut(s) 277, 472, 511
AcyI GRCGYC 1 cut(s) 101
AfaI GTAC 2 cut(s) 362, 469
AfiI CCNNNNNNNGG 6 cut(s) 43, 258, 400, 503, 794, 910
AflIII ACRYGT 1 cut(s) 744
AgeI ACCGGT 2 cut(s) 400, 903
AgsI TTSAA 1 cut(s) 518
AjiI CACGTC 1 cut(s) 745
AjnI CCWGG 1 cut(s) 324
AluBI AGCT 2 cut(s) 243, 416
AluI AGCT 2 cut(s) 243, 416
Alw26I GTCTC 1 cut(s) 977
AlwI GGATC 3 cut(s) 430, 443, 775
Ama87I CYCGRG 2 cut(s) 162, 447
AoxI GGCC 2 cut(s) 249, 895
ApaI GGGCCC 1 cut(s) 253
ApeKI GCWGC 1 cut(s) 660
ApoI RAATTY 3 cut(s) 277, 472, 511
AsiGI ACCGGT 2 cut(s) 400, 903
AspLEI GCGC 2 cut(s) 428, 655
AspS9I GGNCC 2 cut(s) 249, 250
AsuC2I CCSGG 2 cut(s) 369, 762
AsuHPI GGTGA 4 cut(s) 166, 215, 390, 822
AvaI CYCGRG 2 cut(s) 162, 447
AxyI CCTNAGG 1 cut(s) 257
BaeGI GKGCMC 1 cut(s) 253
BaeI ACNNNNGTAYC 2 cut(s) 684, 717
BamHI GGATCC 1 cut(s) 435
BanI GGYRCC 1 cut(s) 195
BanII GRGCYC 2 cut(s) 253, 456
BbsI GAAGAC 1 cut(s) 738
BbvI GCAGC 1 cut(s) 672
BccI CCATC 2 cut(s) 85, 134
BceAI ACGGC 1 cut(s) 209
BciT130I CCWGG 1 cut(s) 326
BclI TGATCA 1 cut(s) 865
BcnI CCSGG 2 cut(s) 369, 762
BcoDI GTCTC 1 cut(s) 977
BfuAI ACCTGC 1 cut(s) 654
BisI GCNGC 2 cut(s) 651, 661
BlpI GCTNAGC 1 cut(s) 417
BlsI GCNGC 2 cut(s) 652, 662
Bme1390I CCNGG 3 cut(s) 326, 369, 762
BmeT110I CYCGRG 2 cut(s) 162, 447
BmgBI CACGTC 1 cut(s) 745
BmgT120I GGNCC 2 cut(s) 249, 250
BmiI GGNNCC 6 cut(s) 197, 250, 251, 437, 649, 916
BmrFI CCNGG 3 cut(s) 326, 369, 762
BmsI GCATC 1 cut(s) 104
BpiI GAAGAC 1 cut(s) 738
Bpu1102I GCTNAGC 1 cut(s) 417
BpuEI CTTGAG 1 cut(s) 31
BpuMI CCSGG 2 cut(s) 369, 762
BsaAI YACGTR 1 cut(s) 138
BsaHI GRCGYC 1 cut(s) 101
BsaJI CCNNGG 3 cut(s) 409, 642, 821
BsaWI WCCGGW 3 cut(s) 110, 400, 903
BsaXI ACNNNNNCTCC 2 cut(s) 11, 41
Bsc4I CCNNNNNNNGG 6 cut(s) 43, 258, 400, 503, 794, 910
Bse118I RCCGGY 2 cut(s) 400, 903
Bse1I ACTGG 1 cut(s) 105
Bse21I CCTNAGG 1 cut(s) 257
BseBI CCWGG 1 cut(s) 326
BseDI CCNNGG 3 cut(s) 409, 642, 821
BseGI GGATG 1 cut(s) 184
BseLI CCNNNNNNNGG 6 cut(s) 43, 258, 400, 503, 794, 910
BseNI ACTGG 1 cut(s) 105
BseSI GKGCMC 1 cut(s) 253
BseXI GCAGC 1 cut(s) 672
Bsh1236I CGCG 2 cut(s) 426, 653
Bsh1285I CGRYCG 3 cut(s) 115, 720, 799
BshFI GGCC 2 cut(s) 251, 897
BshNI GGYRCC 1 cut(s) 195
BshTI ACCGGT 2 cut(s) 400, 903
BsiEI CGRYCG 3 cut(s) 115, 720, 799
BsiHKCI CYCGRG 2 cut(s) 162, 447
BsiSI CCGG 5 cut(s) 111, 368, 401, 762, 904
BslFI GGGAC 3 cut(s) 733, 744, 830
BslI CCNNNNNNNGG 6 cut(s) 43, 258, 400, 503, 794, 910
BsmAI GTCTC 1 cut(s) 977
BsmFI GGGAC 3 cut(s) 733, 744, 830
BsmI GAATGC 1 cut(s) 624
BsnI GGCC 2 cut(s) 251, 897
BsoBI CYCGRG 2 cut(s) 162, 447
Bsp120I GGGCCC 1 cut(s) 249
Bsp1286I GDGCHC 2 cut(s) 253, 456
Bsp1407I TGTACA 2 cut(s) 360, 467
Bsp143I GATC 5 cut(s) 21, 435, 717, 767, 865
Bsp1720I GCTNAGC 1 cut(s) 417
BspACI CCGC 5 cut(s) 651, 795, 839, 943, 963
BspANI GGCC 2 cut(s) 251, 897
BspFNI CGCG 2 cut(s) 426, 653
BspLI GGNNCC 6 cut(s) 197, 250, 251, 437, 649, 916
BspMI ACCTGC 1 cut(s) 654
BspPI GGATC 3 cut(s) 430, 443, 775
BspT107I GGYRCC 1 cut(s) 195
BsrFI RCCGGY 2 cut(s) 400, 903
BsrGI TGTACA 2 cut(s) 360, 467
BsrI ACTGG 1 cut(s) 105
BssAI RCCGGY 2 cut(s) 400, 903
BssECI CCNNGG 3 cut(s) 409, 642, 821
BssMI GATC 5 cut(s) 21, 435, 717, 767, 865
BssNI GRCGYC 1 cut(s) 101
BssT1I CCWWGG 2 cut(s) 642, 821
Bst2UI CCWGG 1 cut(s) 326
Bst4CI ACNGT 6 cut(s) 38, 737, 758, 781, 834, 971
BstACI GRCGYC 1 cut(s) 101
BstAPI GCANNNNNTGC 1 cut(s) 621
BstAUI TGTACA 2 cut(s) 360, 467
BstBAI YACGTR 1 cut(s) 138
BstC8I GCNNGC 1 cut(s) 947
BstDEI CTNAG 2 cut(s) 257, 417
BstF5I GGATG 1 cut(s) 184
BstFNI CGCG 2 cut(s) 426, 653
BstHHI GCGC 2 cut(s) 428, 655
BstKTI GATC 5 cut(s) 24, 438, 720, 770, 868
BstMAI GTCTC 1 cut(s) 977
BstMBI GATC 5 cut(s) 21, 435, 717, 767, 865
BstMCI CGRYCG 3 cut(s) 115, 720, 799
BstMWI GCNNNNNNNGC 1 cut(s) 621
BstNI CCWGG 1 cut(s) 326
BstSCI CCNGG 3 cut(s) 324, 367, 760
BstSLI GKGCMC 1 cut(s) 253
BstUI CGCG 2 cut(s) 426, 653
BstV1I GCAGC 1 cut(s) 672
BstV2I GAAGAC 1 cut(s) 738
BstX2I RGATCY 1 cut(s) 435
BstYI RGATCY 1 cut(s) 435
Bsu36I CCTNAGG 1 cut(s) 257
BsuRI GGCC 2 cut(s) 251, 897
BtgZI GCGATG 1 cut(s) 938
BtrI CACGTC 1 cut(s) 745
BtsCI GGATG 1 cut(s) 184
BtsI GCAGTG 1 cut(s) 486
BtsIMutI CAGTG 1 cut(s) 486
BveI ACCTGC 1 cut(s) 654
Cac8I GCNNGC 1 cut(s) 947
CfoI GCGC 2 cut(s) 428, 655
Cfr10I RCCGGY 2 cut(s) 400, 903
Cfr13I GGNCC 2 cut(s) 249, 250
Csp6I GTAC 2 cut(s) 361, 468
CspAI ACCGGT 2 cut(s) 400, 903
CviAII CATG 2 cut(s) 172, 791
CviJI RGCY 8 cut(s) 243, 251, 408, 416, 421, 454, 650, 897
CviKI_1 RGCY 8 cut(s) 243, 251, 408, 416, 421, 454, 650, 897
CviQI GTAC 2 cut(s) 361, 468
DdeI CTNAG 2 cut(s) 257, 417
DpnI GATC 5 cut(s) 23, 437, 719, 769, 867
DpnII GATC 5 cut(s) 21, 435, 717, 767, 865
EciI GGCGGA 2 cut(s) 854, 932
Eco130I CCWWGG 2 cut(s) 642, 821
Eco24I GRGCYC 2 cut(s) 253, 456
Eco32I GATATC 1 cut(s) 858
Eco81I CCTNAGG 1 cut(s) 257
Eco88I CYCGRG 2 cut(s) 162, 447
EcoO109I RGGNCCY 1 cut(s) 249
EcoRII CCWGG 1 cut(s) 324
EcoRV GATATC 1 cut(s) 858
EcoT14I CCWWGG 2 cut(s) 642, 821
EcoT38I GRGCYC 2 cut(s) 253, 456
ErhI CCWWGG 2 cut(s) 642, 821
FaeI CATG 2 cut(s) 175, 794
FaqI GGGAC 3 cut(s) 733, 744, 830
FatI CATG 2 cut(s) 171, 790
FauNDI CATATG 1 cut(s) 357
FbaI TGATCA 1 cut(s) 865
Fnu4HI GCNGC 2 cut(s) 651, 661
FokI GGATG 1 cut(s) 191
FriOI GRGCYC 2 cut(s) 253, 456
Fsp4HI GCNGC 2 cut(s) 651, 661
GlaI GCGC 2 cut(s) 427, 654
GluI GCNGC 2 cut(s) 651, 661
HaeIII GGCC 2 cut(s) 251, 897
HapII CCGG 5 cut(s) 111, 368, 401, 762, 904
HhaI GCGC 2 cut(s) 428, 655
Hin1I GRCGYC 1 cut(s) 101
Hin1II CATG 2 cut(s) 175, 794
Hin6I GCGC 2 cut(s) 426, 653
HinP1I GCGC 2 cut(s) 426, 653
HincII GTYRAC 1 cut(s) 742
HindII GTYRAC 1 cut(s) 742
HinfI GANTC 5 cut(s) 160, 236, 390, 634, 827
HpaII CCGG 5 cut(s) 111, 368, 401, 762, 904
HphI GGTGA 4 cut(s) 166, 215, 390, 822
Hpy166II GTNNAC 2 cut(s) 394, 742
Hpy188I TCNGA 4 cut(s) 86, 387, 413, 882
Hpy188III TCNNGA 2 cut(s) 164, 349
Hpy8I GTNNAC 2 cut(s) 394, 742
Hpy99I CGWCG 4 cut(s) 103, 731, 882, 896
HpyAV CCTTC 2 cut(s) 169, 523
HpyCH4III ACNGT 6 cut(s) 38, 737, 758, 781, 834, 971
HpyCH4IV ACGT 3 cut(s) 101, 137, 744
HpyCH4V TGCA 4 cut(s) 171, 484, 624, 981
HpyF10VI GCNNNNNNNGC 1 cut(s) 621
HpyF3I CTNAG 2 cut(s) 257, 417
HpySE526I ACGT 3 cut(s) 101, 137, 744
Hsp92I GRCGYC 1 cut(s) 101
Hsp92II CATG 2 cut(s) 175, 794
HspAI GCGC 2 cut(s) 426, 653
Ksp22I TGATCA 1 cut(s) 865
Kzo9I GATC 5 cut(s) 21, 435, 717, 767, 865
LmnI GCTCC 2 cut(s) 413, 647
Lsp1109I GCAGC 1 cut(s) 672
LweI GCATC 1 cut(s) 104
MaeII ACGT 3 cut(s) 101, 137, 744
MaeIII GTNAC 2 cut(s) 568, 781
MalI GATC 5 cut(s) 23, 437, 719, 769, 867
MboI GATC 5 cut(s) 21, 435, 717, 767, 865
MboII GAAGA 1 cut(s) 743
MflI RGATCY 1 cut(s) 435
MhlI GDGCHC 2 cut(s) 253, 456
MluCI AATT 8 cut(s) 277, 299, 312, 375, 472, 485, 511, 619
MlyI GAGTC 3 cut(s) 154, 399, 643
MmeI TCCRAC 1 cut(s) 860
MnlI CCTC 9 cut(s) 144, 419, 432, 443, 456, 515, 605, 758, 805
MseI TTAA 3 cut(s) 339, 501, 599
MspI CCGG 5 cut(s) 111, 368, 401, 762, 904
MspR9I CCNGG 3 cut(s) 326, 369, 762
Mva1269I GAATGC 1 cut(s) 624
MvaI CCWGG 1 cut(s) 326
MvnI CGCG 2 cut(s) 426, 653
MwoI GCNNNNNNNGC 1 cut(s) 621
NciI CCSGG 2 cut(s) 369, 762
NdeI CATATG 1 cut(s) 357
NdeII GATC 5 cut(s) 21, 435, 717, 767, 865
NlaIII CATG 2 cut(s) 175, 794
NlaIV GGNNCC 6 cut(s) 197, 250, 251, 437, 649, 916
NmeAIII GCCGAG 1 cut(s) 681
PaeR7I CTCGAG 1 cut(s) 447
PaqCI CACCTGC 1 cut(s) 654
PcsI WCGNNNNNNNCGW 2 cut(s) 634, 726
PctI GAATGC 1 cut(s) 624
PfeI GAWTC 2 cut(s) 236, 827
PfoI TCCNGGA 1 cut(s) 760
PinAI ACCGGT 2 cut(s) 400, 903
PkrI GCNGC 2 cut(s) 652, 662
Ple19I CGATCG 1 cut(s) 720
PleI GAGTC 3 cut(s) 154, 398, 642
PpsI GAGTC 3 cut(s) 154, 398, 642
Ppu21I YACGTR 1 cut(s) 138
Psp6I CCWGG 1 cut(s) 324
PspGI CCWGG 1 cut(s) 324
PspN4I GGNNCC 6 cut(s) 197, 250, 251, 437, 649, 916
PspOMI GGGCCC 1 cut(s) 249
PspPI GGNCC 2 cut(s) 249, 250
PspXI VCTCGAGB 1 cut(s) 447
PsrI GAACNNNNNNTAC 2 cut(s) 344, 376
PsuI RGATCY 1 cut(s) 435
PvuI CGATCG 1 cut(s) 720
RsaI GTAC 2 cut(s) 362, 469
RsaNI GTAC 2 cut(s) 361, 468
SaqAI TTAA 3 cut(s) 339, 501, 599
SatI GCNGC 2 cut(s) 651, 661
Sau3AI GATC 5 cut(s) 21, 435, 717, 767, 865
Sau96I GGNCC 2 cut(s) 249, 250
SchI GAGTC 3 cut(s) 154, 399, 643
ScrFI CCNGG 3 cut(s) 326, 369, 762
SduI GDGCHC 2 cut(s) 253, 456
SetI ASST 8 cut(s) 104, 140, 245, 258, 327, 418, 668, 747
SfaNI GCATC 1 cut(s) 104
Sfr274I CTCGAG 1 cut(s) 447
SlaI CTCGAG 1 cut(s) 447
SmlI CTYRAG 2 cut(s) 10, 447
SmoI CTYRAG 2 cut(s) 10, 447
Sse9I AATT 8 cut(s) 277, 299, 312, 375, 472, 485, 511, 619
SsiI CCGC 5 cut(s) 651, 795, 839, 943, 963
StyD4I CCNGG 3 cut(s) 324, 367, 760
StyI CCWWGG 2 cut(s) 642, 821
TaaI ACNGT 6 cut(s) 38, 737, 758, 781, 834, 971
TaiI ACGT 3 cut(s) 104, 140, 747
TaqI TCGA 9 cut(s) 70, 98, 115, 239, 350, 448, 637, 716, 729
TasI AATT 8 cut(s) 277, 299, 312, 375, 472, 485, 511, 619
TatI WGTACW 2 cut(s) 360, 467
TauI GCSGC 1 cut(s) 653
TfiI GAWTC 2 cut(s) 236, 827
Tru1I TTAA 3 cut(s) 339, 501, 599
Tru9I TTAA 3 cut(s) 339, 501, 599
TscAI CASTG 1 cut(s) 486
TseI GCWGC 1 cut(s) 660
TspDTI ATGAA 2 cut(s) 188, 347
TspRI CASTG 1 cut(s) 486
XapI RAATTY 3 cut(s) 277, 472, 511
XhoI CTCGAG 1 cut(s) 447
ZraI GACGTC 1 cut(s) 102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.