Rorug01G0433400
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0006965
Physical Location & Seq
Forward (+)
53204075 .. 53213325
9251 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0433400.1

Sequence Viewer

Length: 4854 bp
ATGGCCGCTTCTTCTTCCTCTGCATCTGTCGGTCGTTGGAACTACGATGTCTTCATCAGTTTTAGAGGCGATGACACTCGCAAGAACTTTGTCGGCCATCTCTACAAAGCTCTCCGTCAGAAAGCAATCAACACCTTCATCGATTCCGACGAGCTTCGAACAGGAAACGACCCTTCAGAGCTTCTGGAAGCCATCGCCGACTCGAGGCTTTCCGTTGTGGTGTTCTCCAAACACTATGCTTCTTCCAAGTGGTGCCTCAAAGAGCTGGTCAAGATCGTGGAGTGCATGAAAACGATGAAGCAAATTGTGGTGCCCGTTTTCTACGAACTTGATGCTTCTGATATTCGGAAACCCAAAGCGAGTTTTGAGCAAGCTTTTGCTGAACATACGCATGTGGAAGAGGTGCAGAGGTGGAAGTCTGCTCTAGTTGAGGCCTCCAATTTATCTGGCTGGGATTCAATAAATTATGAGGATGACGCCAAGCTTATTGAGAAAATAGTCGGTGATATTTCTAAAAGATTGATCCGCGTCTCATCCGGTAAAGACAATGTTTTGGTTGGAATGGATAAACACATTGCAGAAATAGATGTACTATTGTGTCTTGGGGTGGATGATGTTCGTGGTATTGGAATATTCGGAATGTCTGGTATAGGTAAAACAACCATAGCTCGAGCTGTTTATGATGAAATCACTTGTCAGTTTGAACACTATTGCTTTCTTGAAAATGTCAAAGATGGTTTCAAGAATAAAGGTGCGGTACGTATGCAGGAAGAACTTCTATCTAGAATCCTGAAGAAAAAGGTGTGTACCTTAGGTAATTTGAATACAGGCTTCAAGATGGTTATGGAAAGGCTGAATAAGAAAAGAGTTTTGCTTGTGCTTGATGATGTGGAAAATTTTGCCCAAATTGAAGCCTTACTCGGAAAGCAATGTTCATTTGGTGGTGGAAGTAGAATAATTATAACAACTAGAGATATGCAATCACTAAGTGGAGTGCAGGAGAGATATAGTCCCAAATTTTTAAGTGATGATGAAGATCTTGAGCTTTTTATGCAGTATGCCTTCAGATCACACCAACCTGCAGGAGAGTACGATTCTCTCTCAAGGCGTGCCATAGAATATGCTCAAGGTCTGCCTTTAGCACTTAAAGTCTTGGGGGCTTTTCTTGATAGCAAAAGTGAATGCGAGTGGGAAGATGAGCTAGAAAAATTAAAGAAAATCCCACACATAGAAATTCAGGGTGTGCTTAGAACAAGCTTTGATGGACTAGATCCTTTACAAAAAGATATATTTCTAGATATTGCATGCTTCTTCAGAGGAATGGACAAAGGATATGTAACAAAAGTTCTGGAAAGTTGTGGCTTTTACCCCCATAGTGGATTACGAGTACTACTCGATAGAGCTCTCATCACGATCTCAGATGACAATAGGCTCGAAATGCATGATTTACTACAAGAAATAGGTTGGGAAATCGTCCGACGACAATCAATTAAAGAACCTGGGAAGCGCACTAGGTTGTGGGTTTTTGAAGATGTCAATCATGTGTTCACTCAAAATACGGCTACAGATGCAGTTGAGGGCATAATGCTTGACTTGTCAAGATCTAAAGAGGTATACTTAAATTCTGAAGCTTTTGTTAAGATGACGAGGCTGAGACTGCTAAGAATTATCAGTGATAACCATTCACCTCAGGATTTATGTCATCCAAGTGATGTCTGTCAACAACATCTGAGTGGGAACTTCAGGTTTCTTTCTAATGAGTTGAGGATTCTCCACTGGCATGGATTCCCCTCAAAGTCTTTGCCGTCCAATTTTGAACCGACGAATCTTGTTGAACTTGACATGCATTCTAGTCACATTGAACAACTGTGGGAAGGAATCAAGCCTCTGAAAAAGTTGACAATTATTAATCTAAGTCACTCTCAATACCTTAAGAAAATCCCCGACTTCACTGAGGCGACAAATATGAAGAAACTAATTCTTGATGGTTGTTCAAGTTTATTGGAGGTTCATCCGTCAATTTCAGCTCTTAAAAACCTTGTTGTCATGAGTCTAAAAGGGTGCAAAGCAGTTAAGAATCTTCCGGATAGCATTTACATGAAATCTCTTAAAACCCTTGATCTTTCTGGCTGCTCAAATCTTAAGATGTTTCCAGAGATCTCAGGAATTATGGATGACCTATCGGAGCTTTATTTAAATGAGACTGCAATTGAAGAACTACCGTCATCGATTGAACAGCTTCAGGGGCTAGTGTTATTGAATATGGCAGACTGCAGAAGCCTTGTCTGTCTTCCAGACAATATCTGTAATTTGGCATACCTTACAAATCTCGCTCTCTCTGGGTGCTCAAAACTTAATCGTTTGCCTGAGAACTTGGGGAATTTAGAATCCTTGATGGTCCTTGAAATAGAAAGAAGTGGTATAAAAGAACTCCCTTTTTCGATCTTACATGTGAATAAGCTCAAAAGATTATCATGTGATGGATGTAAAGAAATGATGGCACCCCTTTCATCATGGTCATCATCAATCAAAGGATATTATACTTACTCTGGTATGCTACATCTTGATTTAAGTGACTGCAATCTGATGGTATTATCAGATGGTATTGCTCATTTGTCCTCATTAAGAACGTTAGAGCTGCGCAGAAACAACTTGGAGAGCTTACCTGCAGCAATGAATCGACTTCGTTGTTTATCACATCTTGAATTAGAAGCGTGCAAGAAACTGAAATCAATACCTGAGCTTTCATCAAGTATTAGTTACATAGATGCACATGATTGCACAGCTTTGGAAACAGTTTCAACACCAAAGCCTCGCTCTTCTACAAATCTCTGCTTCACATTTTCAAATTGTCTCCAGCTGATACAAACAAATCTATTCAGGGATATTGTGGAAACTCATTCACATTATCAGGGTAGCTATCTACGACCTCTTTCCTTTAGTATGTCACTTCCTGGAAGTGAAATTCCTCATTGGTTCAACCATCAGAGTAAGGGATTCTCAGTGACTGTGCAGCTACCACAGAATTGGTTCGATGATAAGTTTTTGGGATTTGCTATATGCGGTGTTAGTAACTTCAAGGGTGCTCATAATGATGCATCCGATCTATCTGCTGTTTGTTTTTGTACCTTCAAAGGAAATCATGGTGAGTACAAGTTCAGTTTTCATTTGCTTGATTGGGGTTTCAGAACAAACAGATACCTCCAGTCGGATCACATGTTCATGGGATATGTGCCATGGTCACAATATCGCTTCAGTGATGAAGGGAAGCAGGGCAATGAACGCTACTACACTGAAGTCACATTTGAGATTGTGGTAGAAAATGGAGTCTATGCACATGATGATTACAGGACAGTAATACGACGACATTGCATCACAAGTTGCGGAGTGCGTTTGTTTCATGGTAATTATATGGGACTGCAAAATCTAAGCATCACTCAGCCTAATTCTCATCATGATGGCTGTGAAATCCCATTGGACACTGTGAGGAAGAGAAAGAGAGATATAGCAGGGGTGACATTGCAGTGGGAGTGGCCTCCCGCAGAAGAGGTGTTCACTGAGCAAGAGCAAGAAGGATCCCCATCAAGGGAAGGGTCCCTGGCGGGGGAAGGGTCCTCCGCGGGCAAGAGGTCCTCGGCGGGGGAAGGGGTTCCTTCTATTCTTACTGCAGTAGCAACAGTAGAAAGTGGTGCCAATGAACGTGATGGTCCAACTATCCCTAATCGTACTAACAGAAGAAGGCGCACAAATTTGCGAAATTCGGCAACGTGGATCCGAGATACAAATCAACTTATTGATGACGGCCATGCTTGGAGAAAGTATGGCCAAAAGGAGATACTTGGATCCAGATATCCCAGAAACTATTACAGATGCACTTATCGAATGGATCAAGGCTGTATGGCCACTAAGCACGTGCAACGCATCAGTGACGAGCCAGAAGAGAAACTGTTAAAATATTGGTATGTCAATGGCATTGAACACTACAAACTCACTTGCGCTGTTATTGACACTCGCCATTTAACCGTCATAAGAGGAAAGCAGGATACAAAAAGGGAGTCCTTGCAACATATGGATGGTGGAATGTTTTCTCATATTTTTAATGCCTTGAAACCAGTGATAACATTACAACCATCTCTTATTTCCATCATAGGAAGAGAAGCAAACCAAGATGAGTGCTTGATGCTAGCTGAAGGTGGAATTTGGGATGCCCTATCCAGTGGCATGGTGGCTATAACGTGCTTCTTTCATCCGAGCAATGAAATGTTTTTCTCTTCTAACAGCAACCTCGCAGCTCTGCAACATTCTGCTGCCTCGCATTGGGGCAAGGAAGTGCCATTTAACCCACCACTTGCACCTACGGTCCAGGCCCATCACATACGCCTGCACAGTCACAAACTCCAATACATACATTCTGCTCTGTTCAAAACTTCAAAATGTCGACAGTCATCGGAATCTGGGACTCCGGTCCAACCAGCTCTGCAACAGAAAGCAATCAATACTTTCATTGATTCTGAAGAGCTGAGTAAAGGCAACAAGATTTCGGAGCTACTCACAGCTATTGAGGACTCGAGGATTTCGATTGTAGTTTTATCTCAGAACTATGCCACTTCCACTTGGTGCTTAAAAGAGCTCGTCAAAATCCTGGATTGCATGGTAACAAAGAAGCATATAGTGGTGCCCATCTTCTACGAAGTAGATCCCTCTCATCTACGGAAAATCAATGGAAAATTTGCAGAAGCTTTTGCCAAACATGGAGAAAATCCTAACCTCGACAAGGAAGAGTTGAAGAGCTGGACATCCGCTCTAACTGCAATTGCGGATCTATCTGGCTGGGATTCGAGCAAATATGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC2_membrane ABC_tran ABC_trans_N PDR_assoc

Protein Analysis

1617

Amino Acids

182.81

Weight (kDa)

6.45

Isoelectric Point (pI)

47.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 14 - 182 1.6e-51 TIR domain
TIR_2 PF13676 17 - 118 2e-15 TIR domain
NB-ARC PF00931 204 - 357 2.3e-16 NB-ARC domain
WHD_ROQ1 PF23282 427 - 496 6.3e-22 Disease resistance protein Roq1-like, winged-helix domain
LRR_3 PF07725 609 - 628 1.8e-06 Leucine Rich Repeat
LRR_14 PF23598 669 - 780 8.4e-09 Leucine-rich repeat region
LRR_13 PF23286 700 - 786 8.1e-12 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 767 - 832 3.2e-09 Leucine-rich repeat region
LRR_14 PF23598 854 - 915 3.5e-06 Leucine-rich repeat region
LRR_8 PF13855 854 - 906 2.4e-06 Leucine rich repeat
C-JID PF20160 984 - 1134 6.9e-21 C-JID domain
WRKY PF03106 1266 - 1321 1e-18 WRKY DNA -binding domain
TIR_2 PF13676 1488 - 1573 9.8e-11 TIR domain
TIR PF01582 1491 - 1615 8.7e-37 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 962
Acc16I TGCGCA 1 cut(s) 2642
Acc36I ACCTGC 2 cut(s) 1087, 2674
AccB1I GGYRCC 5 cut(s) 252, 310, 2500, 3689, 4675
AccBSI CCGCTC 1 cut(s) 4802
AccI GTMKAC 2 cut(s) 1614, 4438
AccII CGCG 2 cut(s) 528, 3620
AccIII TCCGGA 1 cut(s) 2083
AclI AACGTT 1 cut(s) 2630
AcoI YGGCCR 5 cut(s) 3, 94, 3802, 3823, 3900
AcvI CACGTG 1 cut(s) 3913
AcyI GRCGYC 1 cut(s) 477
AdeI CACNNNGTG 2 cut(s) 989, 4617
AfaI GTAC 8 cut(s) 591, 759, 808, 1091, 1389, 3127, 3152, 3727
AflII CTTAAG 2 cut(s) 1931, 2141
AflIII ACRYGT 2 cut(s) 2449, 3216
AjnI CCWGG 5 cut(s) 1498, 2953, 3597, 4362, 4641
AloI GAACNNNNNNTCC 2 cut(s) 3203, 3235
Alw21I GWGCWC 4 cut(s) 1405, 2348, 3088, 4632
Alw26I GTCTC 4 cut(s) 535, 1648, 2195, 2858
AlwNI CAGNNNCTG 1 cut(s) 3008
Ama87I CYCGRG 3 cut(s) 202, 669, 4567
Aor13HI TCCGGA 1 cut(s) 2083
AoxI GGCC 8 cut(s) 3, 94, 432, 3533, 3802, 3823, 3900, 4365
ApeKI GCWGC 6 cut(s) 2130, 2638, 2669, 3013, 4289, 4307
ArsI GACNNNNNNTTYG 4 cut(s) 252, 284, 4040, 4072
AseI ATTAAT 1 cut(s) 1908
Asp700I GAANNNNTTC 5 cut(s) 774, 2238, 3029, 3648, 4084
AspLEI GCGC 4 cut(s) 1509, 2643, 3744, 3998
AspS9I GGNCC 8 cut(s) 2398, 3594, 3612, 3630, 3707, 4360, 4366, 4465
AsuHPI GGTGA 4 cut(s) 515, 1677, 3158, 3526
AsuII TTCGAA 1 cut(s) 157
AsuNHI GCTAGC 1 cut(s) 4183
AvaI CYCGRG 3 cut(s) 202, 669, 4567
AvaII GGWCC 7 cut(s) 2398, 3594, 3612, 3630, 3707, 4360, 4465
AxyI CCTNAGG 2 cut(s) 811, 1689
BaeGI GKGCMC 2 cut(s) 315, 4680
BalI TGGCCA 2 cut(s) 3825, 3902
BamHI GGATCC 3 cut(s) 3575, 3771, 3842
BanI GGYRCC 5 cut(s) 252, 310, 2500, 3689, 4675
BanII GRGCYC 2 cut(s) 1405, 4632
BbrPI CACGTG 1 cut(s) 3913
BbsI GAAGAC 2 cut(s) 43, 2282
Bbv12I GWGCWC 4 cut(s) 1405, 2348, 3088, 4632
BbvI GCAGC 6 cut(s) 2117, 2625, 2681, 3025, 4294, 4301
BceAI ACGGC 3 cut(s) 1575, 1789, 3817
BcgI CGANNNNNNTGC 6 cut(s) 314, 348, 3092, 3126, 3351, 3385
BciT130I CCWGG 5 cut(s) 1500, 2955, 3599, 4364, 4643
BciVI GTATCC 1 cut(s) 4036
BcoDI GTCTC 4 cut(s) 535, 1648, 2195, 2858
BfmI CTRYAG 5 cut(s) 1080, 1563, 2272, 2667, 3666
BfrI CTTAAG 2 cut(s) 1931, 2141
BfuAI ACCTGC 2 cut(s) 1087, 2674
BfuI GTATCC 1 cut(s) 4036
BglII AGATCT 3 cut(s) 1036, 1601, 2157
BisI GCNGC 7 cut(s) 6, 2131, 2639, 2670, 3014, 4290, 4308
BlsI GCNGC 7 cut(s) 7, 2132, 2640, 2671, 3015, 4291, 4309
BmcAI AGTACT 1 cut(s) 1389
Bme1390I CCNGG 5 cut(s) 1500, 2955, 3599, 4364, 4643
Bme18I GGWCC 7 cut(s) 2398, 3594, 3612, 3630, 3707, 4360, 4465
BmeT110I CYCGRG 3 cut(s) 202, 669, 4567
BmgT120I GGNCC 8 cut(s) 2398, 3594, 3612, 3630, 3707, 4360, 4366, 4465
BmrFI CCNGG 5 cut(s) 1500, 2955, 3599, 4364, 4643
BmtI GCTAGC 1 cut(s) 4187
BoxI GACNNNNGTC 1 cut(s) 4463
BpiI GAAGAC 2 cut(s) 43, 2282
BplI GAGNNNNNCTC 2 cut(s) 1377, 1409
BpmI CTGGAG 2 cut(s) 2840, 3188
Bpu10I CCTNAGC 1 cut(s) 2739
Bpu14I TTCGAA 1 cut(s) 157
BpuEI CTTGAG 3 cut(s) 1061, 1087, 1110
Bsa29I ATCGAT 2 cut(s) 141, 2228
BsaAI YACGTR 2 cut(s) 761, 3913
BsaBI GATNNNNATC 4 cut(s) 1035, 1536, 3580, 3783
BsaHI GRCGYC 1 cut(s) 477
BsaJI CCNNGG 5 cut(s) 1499, 3236, 3597, 3618, 3633
BsaWI WCCGGW 3 cut(s) 536, 2083, 4462
Bse1I ACTGG 4 cut(s) 1781, 3205, 4112, 4215
Bse21I CCTNAGG 2 cut(s) 811, 1689
Bse3DI GCAATG 7 cut(s) 573, 935, 2679, 3283, 3367, 3518, 4261
Bse8I GATNNNNATC 4 cut(s) 1035, 1536, 3580, 3783
BseAI TCCGGA 1 cut(s) 2083
BseBI CCWGG 5 cut(s) 1500, 2955, 3599, 4364, 4643
BseCI ATCGAT 2 cut(s) 141, 2228
BseDI CCNNGG 5 cut(s) 1499, 3236, 3597, 3618, 3633
BseJI GATNNNNATC 4 cut(s) 1035, 1536, 3580, 3783
BseMI GCAATG 7 cut(s) 573, 935, 2679, 3283, 3367, 3518, 4261
BseNI ACTGG 4 cut(s) 1781, 3205, 4112, 4215
BseSI GKGCMC 2 cut(s) 315, 4680
BseXI GCAGC 6 cut(s) 2117, 2625, 2681, 3025, 4294, 4301
BseYI CCCAGC 2 cut(s) 450, 4830
BsgI GTGCAG 4 cut(s) 425, 1016, 3032, 4367
Bsh1236I CGCG 2 cut(s) 528, 3620
Bsh1285I CGRYCG 1 cut(s) 34
BshFI GGCC 8 cut(s) 5, 96, 434, 3535, 3804, 3825, 3902, 4367
BshNI GGYRCC 5 cut(s) 252, 310, 2500, 3689, 4675
BshVI ATCGAT 2 cut(s) 141, 2228
BsiEI CGRYCG 1 cut(s) 34
BsiHKAI GWGCWC 4 cut(s) 1405, 2348, 3088, 4632
BsiHKCI CYCGRG 3 cut(s) 202, 669, 4567
BsiSI CCGG 3 cut(s) 537, 2084, 4463
BslFI GGGAC 4 cut(s) 996, 3429, 3580, 4471
BsmAI GTCTC 4 cut(s) 535, 1648, 2195, 2858
BsmBI CGTCTC 1 cut(s) 535
BsmFI GGGAC 4 cut(s) 996, 3429, 3580, 4471
BsmI GAATGC 2 cut(s) 1187, 1846
BsnI GGCC 8 cut(s) 5, 96, 434, 3535, 3804, 3825, 3902, 4367
BsoBI CYCGRG 3 cut(s) 202, 669, 4567
Bsp119I TTCGAA 1 cut(s) 157
Bsp1286I GDGCHC 6 cut(s) 315, 1405, 2348, 3088, 4632, 4680
Bsp13I TCCGGA 1 cut(s) 2083
Bsp19I CCATGG 1 cut(s) 3236
BspANI GGCC 8 cut(s) 5, 96, 434, 3535, 3804, 3825, 3902, 4367
BspDI ATCGAT 2 cut(s) 141, 2228
BspEI TCCGGA 1 cut(s) 2083
BspFNI CGCG 2 cut(s) 528, 3620
BspHI TCATGA 2 cut(s) 2046, 3454
BspMAI CTGCAG 4 cut(s) 1084, 2276, 2671, 3670
BspMI ACCTGC 2 cut(s) 1087, 2674
BspOI GCTAGC 1 cut(s) 4187
BspQI GCTCTTC 3 cut(s) 2824, 4509, 4781
BspT104I TTCGAA 1 cut(s) 157
BspT107I GGYRCC 5 cut(s) 252, 310, 2500, 3689, 4675
BspTI CTTAAG 2 cut(s) 1931, 2141
BsrBI CCGCTC 1 cut(s) 4802
BsrDI GCAATG 7 cut(s) 573, 935, 2679, 3283, 3367, 3518, 4261
BsrI ACTGG 4 cut(s) 1781, 3205, 4112, 4215
BssECI CCNNGG 5 cut(s) 1499, 3236, 3597, 3618, 3633
BssNAI GTATAC 1 cut(s) 1615
BssNI GRCGYC 1 cut(s) 477
BssT1I CCWWGG 1 cut(s) 3236
Bst1107I GTATAC 1 cut(s) 1615
Bst2UI CCWGG 5 cut(s) 1500, 2955, 3599, 4364, 4643
BstACI GRCGYC 1 cut(s) 477
BstAFI CTTAAG 2 cut(s) 1931, 2141
BstBAI YACGTR 2 cut(s) 761, 3913
BstBI TTCGAA 1 cut(s) 157
BstC8I GCNNGC 7 cut(s) 372, 1110, 1306, 2716, 3622, 4185, 4382
BstDSI CCRYGG 2 cut(s) 3236, 3618
BstENI CCTNNNNNAGG 1 cut(s) 4772
BstFNI CGCG 2 cut(s) 528, 3620
BstHHI GCGC 4 cut(s) 1509, 2643, 3744, 3998
BstMAI GTCTC 4 cut(s) 535, 1648, 2195, 2858
BstMCI CGRYCG 1 cut(s) 34
BstMWI GCNNNNNNNGC 7 cut(s) 1051, 1438, 1568, 1657, 2245, 3282, 4808
BstNI CCWGG 5 cut(s) 1500, 2955, 3599, 4364, 4643
BstNSI RCATGY 5 cut(s) 395, 1308, 1846, 2453, 3220
BstPAI GACNNNNGTC 1 cut(s) 4463
BstSCI CCNGG 5 cut(s) 1498, 2953, 3597, 4362, 4641
BstSFI CTRYAG 5 cut(s) 1080, 1563, 2272, 2667, 3666
BstSLI GKGCMC 2 cut(s) 315, 4680
BstSNI TACGTA 1 cut(s) 761
BstUI CGCG 2 cut(s) 528, 3620
BstV1I GCAGC 6 cut(s) 2117, 2625, 2681, 3025, 4294, 4301
BstV2I GAAGAC 2 cut(s) 43, 2282
BstX2I RGATCY 9 cut(s) 1036, 1270, 1601, 2157, 3575, 3771, 3842, 4696, 4819
BstXI CCANNNNNNTGG 3 cut(s) 1781, 3027, 4222
BstYI RGATCY 9 cut(s) 1036, 1270, 1601, 2157, 3575, 3771, 3842, 4696, 4819
BstZ17I GTATAC 1 cut(s) 1615
Bsu15I ATCGAT 2 cut(s) 141, 2228
Bsu36I CCTNAGG 2 cut(s) 811, 1689
BsuI GTATCC 1 cut(s) 4036
BsuRI GGCC 8 cut(s) 5, 96, 434, 3535, 3804, 3825, 3902, 4367
BsuTUI ATCGAT 2 cut(s) 141, 2228
BtgI CCRYGG 2 cut(s) 3236, 3618
BtgZI GCGATG 2 cut(s) 84, 178
BtsI GCAGTG 1 cut(s) 3530
BveI ACCTGC 2 cut(s) 1087, 2674
Cac8I GCNNGC 7 cut(s) 372, 1110, 1306, 2716, 3622, 4185, 4382
CaiI CAGNNNCTG 1 cut(s) 3008
CciI TCATGA 2 cut(s) 2046, 3454
CfoI GCGC 4 cut(s) 1509, 2643, 3744, 3998
Cfr13I GGNCC 8 cut(s) 2398, 3594, 3612, 3630, 3707, 4360, 4366, 4465
Cfr42I CCGCGG 1 cut(s) 3621
ClaI ATCGAT 2 cut(s) 141, 2228
CseI GACGC 2 cut(s) 485, 517
Csp6I GTAC 8 cut(s) 590, 758, 807, 1090, 1388, 3126, 3151, 3726
CviQI GTAC 8 cut(s) 590, 758, 807, 1090, 1388, 3126, 3151, 3726
DraI TTTAAA 1 cut(s) 2196
DraIII CACNNNGTG 2 cut(s) 989, 4617
EaeI YGGCCR 5 cut(s) 3, 94, 3802, 3823, 3900
Ecl136II GAGCTC 2 cut(s) 1403, 4630
Eco105I TACGTA 1 cut(s) 761
Eco130I CCWWGG 1 cut(s) 3236
Eco147I AGGCCT 1 cut(s) 434
Eco24I GRGCYC 2 cut(s) 1405, 4632
Eco32I GATATC 1 cut(s) 3851
Eco47I GGWCC 7 cut(s) 2398, 3594, 3612, 3630, 3707, 4360, 4465
Eco53kI GAGCTC 2 cut(s) 1403, 4630
Eco72I CACGTG 1 cut(s) 3913
Eco81I CCTNAGG 2 cut(s) 811, 1689
Eco88I CYCGRG 3 cut(s) 202, 669, 4567
EcoICRI GAGCTC 2 cut(s) 1403, 4630
EcoNI CCTNNNNNAGG 1 cut(s) 4772
EcoO109I RGGNCCY 3 cut(s) 3594, 3612, 3630
EcoRII CCWGG 5 cut(s) 1498, 2953, 3597, 4362, 4641
EcoRV GATATC 1 cut(s) 3851
EcoT14I CCWWGG 1 cut(s) 3236
EcoT22I ATGCAT 3 cut(s) 1443, 1848, 3100
EcoT38I GRGCYC 2 cut(s) 1405, 4632
ErhI CCWWGG 1 cut(s) 3236
Esp3I CGTCTC 1 cut(s) 535
FaqI GGGAC 4 cut(s) 996, 3429, 3580, 4471
FauI CCCGC 4 cut(s) 3547, 3595, 3613, 3631
FauNDI CATATG 1 cut(s) 4068
FblI GTMKAC 2 cut(s) 1614, 4438
Fnu4HI GCNGC 7 cut(s) 6, 2131, 2639, 2670, 3014, 4290, 4308
FriOI GRGCYC 2 cut(s) 1405, 4632
Fsp4HI GCNGC 7 cut(s) 6, 2131, 2639, 2670, 3014, 4290, 4308
FspI TGCGCA 1 cut(s) 2642
GlaI GCGC 4 cut(s) 1508, 2642, 3743, 3997
GluI GCNGC 7 cut(s) 6, 2131, 2639, 2670, 3014, 4290, 4308
GsaI CCCAGC 2 cut(s) 454, 4834
GsuI CTGGAG 2 cut(s) 2840, 3188
HaeIII GGCC 8 cut(s) 5, 96, 434, 3535, 3804, 3825, 3902, 4367
HapII CCGG 3 cut(s) 537, 2084, 4463
HgaI GACGC 2 cut(s) 485, 517
HhaI GCGC 4 cut(s) 1509, 2643, 3744, 3998
Hin1I GRCGYC 1 cut(s) 477
Hin6I GCGC 4 cut(s) 1507, 2641, 3742, 3996
HinP1I GCGC 4 cut(s) 1507, 2641, 3742, 3996
HincII GTYRAC 3 cut(s) 1721, 1899, 4439
HindII GTYRAC 3 cut(s) 1721, 1899, 4439
HindIII AAGCTT 5 cut(s) 372, 482, 1255, 1629, 4737
HpaII CCGG 3 cut(s) 537, 2084, 4463
HphI GGTGA 4 cut(s) 515, 1677, 3158, 3526
Hpy166II GTNNAC 7 cut(s) 807, 1548, 1615, 1721, 1899, 3555, 4439
Hpy8I GTNNAC 7 cut(s) 807, 1548, 1615, 1721, 1899, 3555, 4439
Hpy99I CGWCG 4 cut(s) 152, 1482, 1825, 3366
HpyCH4IV ACGT 6 cut(s) 760, 2630, 3700, 3767, 3912, 4235
HpyF10VI GCNNNNNNNGC 7 cut(s) 1051, 1438, 1568, 1657, 2245, 3282, 4808
HpySE526I ACGT 6 cut(s) 760, 2630, 3700, 3767, 3912, 4235
Hsp92I GRCGYC 1 cut(s) 477
HspAI GCGC 4 cut(s) 1507, 2641, 3742, 3996
KflI GGGWCCC 1 cut(s) 3594
Kpn2I TCCGGA 1 cut(s) 2083
KspI CCGCGG 1 cut(s) 3621
LguI GCTCTTC 3 cut(s) 2824, 4509, 4781
LmnI GCTCC 2 cut(s) 2185, 4543
Lsp1109I GCAGC 6 cut(s) 2117, 2625, 2681, 3025, 4294, 4301
MaeII ACGT 6 cut(s) 760, 2630, 3700, 3767, 3912, 4235
MbiI CCGCTC 1 cut(s) 4802
MfeI CAATTG 2 cut(s) 2208, 4812
MflI RGATCY 9 cut(s) 1036, 1270, 1601, 2157, 3575, 3771, 3842, 4696, 4819
MhlI GDGCHC 6 cut(s) 315, 1405, 2348, 3088, 4632, 4680
MlsI TGGCCA 2 cut(s) 3825, 3902
MluNI TGGCCA 2 cut(s) 3825, 3902
MlyI GAGTC 6 cut(s) 194, 2059, 3336, 4064, 4453, 4559
MmeI TCCRAC 7 cut(s) 17, 171, 538, 1501, 3189, 3734, 4492
Mox20I TGGCCA 2 cut(s) 3825, 3902
Mph1103I ATGCAT 3 cut(s) 1443, 1848, 3100
MroI TCCGGA 1 cut(s) 2083
MroXI GAANNNNTTC 5 cut(s) 774, 2238, 3029, 3648, 4084
MscI TGGCCA 2 cut(s) 3825, 3902
Msp20I TGGCCA 2 cut(s) 3825, 3902
MspA1I CMGCKG 2 cut(s) 2860, 3620
MspCI CTTAAG 2 cut(s) 1931, 2141
MspI CCGG 3 cut(s) 537, 2084, 4463
MspR9I CCNGG 5 cut(s) 1500, 2955, 3599, 4364, 4643
MunI CAATTG 2 cut(s) 2208, 4812
Mva1269I GAATGC 2 cut(s) 1187, 1846
MvaI CCWGG 5 cut(s) 1500, 2955, 3599, 4364, 4643
MvnI CGCG 2 cut(s) 528, 3620
MwoI GCNNNNNNNGC 7 cut(s) 1051, 1438, 1568, 1657, 2245, 3282, 4808
NcoI CCATGG 1 cut(s) 3236
NdeI CATATG 1 cut(s) 4068
NheI GCTAGC 1 cut(s) 4183
NmeAIII GCCGAG 1 cut(s) 3614
NsbI TGCGCA 1 cut(s) 2642
NsiI ATGCAT 3 cut(s) 1443, 1848, 3100
NspI RCATGY 5 cut(s) 395, 1308, 1846, 2453, 3220
NspV TTCGAA 1 cut(s) 157
PaeI GCATGC 1 cut(s) 1308
PaeR7I CTCGAG 3 cut(s) 202, 669, 4567
PagI TCATGA 2 cut(s) 2046, 3454
PceI AGGCCT 1 cut(s) 434
PciI ACATGT 2 cut(s) 2449, 3216
PciSI GCTCTTC 3 cut(s) 2824, 4509, 4781
PcsI WCGNNNNNNNCGW 2 cut(s) 147, 4574
PctI GAATGC 2 cut(s) 1187, 1846
PdmI GAANNNNTTC 5 cut(s) 774, 2238, 3029, 3648, 4084
PfoI TCCNGGA 2 cut(s) 2953, 4641
PkrI GCNGC 7 cut(s) 7, 2132, 2640, 2671, 3015, 4291, 4309
PleI GAGTC 6 cut(s) 194, 2058, 3335, 4063, 4453, 4559
PmaCI CACGTG 1 cut(s) 3913
PmlI CACGTG 1 cut(s) 3913
PpsI GAGTC 6 cut(s) 194, 2058, 3335, 4063, 4453, 4559
Ppu21I YACGTR 2 cut(s) 761, 3913
PpuMI RGGWCCY 3 cut(s) 3594, 3612, 3630
PscI ACATGT 2 cut(s) 2449, 3216
PshAI GACNNNNGTC 1 cut(s) 4463
PshBI ATTAAT 1 cut(s) 1908
PsiI TTATAA 1 cut(s) 962
Psp124BI GAGCTC 2 cut(s) 1405, 4632
Psp1406I AACGTT 1 cut(s) 2630
Psp5II RGGWCCY 3 cut(s) 3594, 3612, 3630
Psp6I CCWGG 5 cut(s) 1498, 2953, 3597, 4362, 4641
PspCI CACGTG 1 cut(s) 3913
PspFI CCCAGC 2 cut(s) 450, 4830
PspGI CCWGG 5 cut(s) 1498, 2953, 3597, 4362, 4641
PspPI GGNCC 8 cut(s) 2398, 3594, 3612, 3630, 3707, 4360, 4366, 4465
PspPPI RGGWCCY 3 cut(s) 3594, 3612, 3630
PspXI VCTCGAGB 3 cut(s) 202, 669, 4567
PsrI GAACNNNNNNTAC 2 cut(s) 1329, 1361
PstI CTGCAG 4 cut(s) 1084, 2276, 2671, 3670
PstNI CAGNNNCTG 1 cut(s) 3008
PsuI RGATCY 9 cut(s) 1036, 1270, 1601, 2157, 3575, 3771, 3842, 4696, 4819
PvuII CAGCTG 1 cut(s) 2860
RsaI GTAC 8 cut(s) 591, 759, 808, 1091, 1389, 3127, 3152, 3727
RsaNI GTAC 8 cut(s) 590, 758, 807, 1090, 1388, 3126, 3151, 3726
SacI GAGCTC 2 cut(s) 1405, 4632
SacII CCGCGG 1 cut(s) 3621
SalI GTCGAC 1 cut(s) 4437
SapI GCTCTTC 3 cut(s) 2824, 4509, 4781
SatI GCNGC 7 cut(s) 6, 2131, 2639, 2670, 3014, 4290, 4308
Sau96I GGNCC 8 cut(s) 2398, 3594, 3612, 3630, 3707, 4360, 4366, 4465
SbfI CCTGCAGG 1 cut(s) 1084
ScaI AGTACT 1 cut(s) 1389
SchI GAGTC 6 cut(s) 194, 2059, 3336, 4064, 4453, 4559
ScrFI CCNGG 5 cut(s) 1500, 2955, 3599, 4364, 4643
SdaI CCTGCAGG 1 cut(s) 1084
SduI GDGCHC 6 cut(s) 315, 1405, 2348, 3088, 4632, 4680
SfcI CTRYAG 5 cut(s) 1080, 1563, 2272, 2667, 3666
Sfr274I CTCGAG 3 cut(s) 202, 669, 4567
Sfr303I CCGCGG 1 cut(s) 3621
SfuI TTCGAA 1 cut(s) 157
SgrBI CCGCGG 1 cut(s) 3621
SinI GGWCC 7 cut(s) 2398, 3594, 3612, 3630, 3707, 4360, 4465
SlaI CTCGAG 3 cut(s) 202, 669, 4567
SmiI ATTTAAAT 1 cut(s) 2196
SmlI CTYRAG 8 cut(s) 202, 669, 1040, 1102, 1125, 1931, 2141, 4567
SmoI CTYRAG 8 cut(s) 202, 669, 1040, 1102, 1125, 1931, 2141, 4567
SnaBI TACGTA 1 cut(s) 761
SphI GCATGC 1 cut(s) 1308
Sse8387I CCTGCAGG 1 cut(s) 1084
SseBI AGGCCT 1 cut(s) 434
SspI AATATT 2 cut(s) 633, 3956
SstI GAGCTC 2 cut(s) 1405, 4632
StuI AGGCCT 1 cut(s) 434
StyD4I CCNGG 5 cut(s) 1498, 2953, 3597, 4362, 4641
StyI CCWWGG 1 cut(s) 3236
SwaI ATTTAAAT 1 cut(s) 2196
TaiI ACGT 6 cut(s) 763, 2633, 3703, 3770, 3915, 4238
TaqII GACCGA 1 cut(s) 20
TatI WGTACW 3 cut(s) 589, 1387, 3150
TauI GCSGC 1 cut(s) 8
TseI GCWGC 6 cut(s) 2130, 2638, 2669, 3013, 4289, 4307
TspGWI ACGGA 4 cut(s) 104, 202, 2004, 4726
Vha464I CTTAAG 2 cut(s) 1931, 2141
VpaK11BI GGWCC 7 cut(s) 2398, 3594, 3612, 3630, 3707, 4360, 4465
VspI ATTAAT 1 cut(s) 1908
XagI CCTNNNNNAGG 1 cut(s) 4772
XbaI TCTAGA 2 cut(s) 782, 1294
XceI RCATGY 5 cut(s) 395, 1308, 1846, 2453, 3220
XcmI CCANNNNNNNNNTGG 1 cut(s) 4222
XhoI CTCGAG 3 cut(s) 202, 669, 4567
XmiI GTMKAC 2 cut(s) 1614, 4438
XmnI GAANNNNTTC 5 cut(s) 774, 2238, 3029, 3648, 4084
ZrmI AGTACT 1 cut(s) 1389
Zsp2I ATGCAT 3 cut(s) 1443, 1848, 3100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.