Rroxscaffold_5G00337660

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0006965
Physical Location & Seq
Forward (+)
5827289 .. 5827879
591 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00337660.1

Sequence Viewer

Length: 486 bp
ATGGACTTTCGAACAAACGCTTTATACCTTTTCGGGTGGGCCCTCGCTAGGCCCCCCAAGGAGTCCCCCATTCTCTCGGCTAATATAGACCTCCGTTTGGCCGGTGTATTGTTTGTTGAGGGGAGCCGCACCGAGCGAGAGGGATCATGTTCGATTGTAACGCCGCGTGGCGGTCATTGTCGAAGTGAGGCATTGCCTTGGGAATCGCCGTTGGCGAAGTCCCTCCGCCGATATCAAGTGATAGGCGTTGTCGCCGGAAAATGCATGTCCGCTTCACCGGTGCATGGTGTAACGGTTAGCGTGTTGGGCTCCGTTGCGAGGTATAGGGAGAAGTTCCGTAGTGGAGTTGTGCTTAGTGGAGACCGTCTCACCGCAAGATGGAAGGGGAGAAGTTCGCTAGCGGAGTTGTGCCTAGTGGAGACTAACACACTTGCAAGATGGAAAGACGGATGTTCCGCTAGTGGAGTTGCGCTTAGCGGAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

161

Amino Acids

17.37

Weight (kDa)

9.66

Isoelectric Point (pI)

53.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 166
AciI CCGC 9 cut(s) 127, 164, 171, 226, 270, 372, 401, 456, 477
AclWI GGATC 1 cut(s) 151
AcoI YGGCCR 1 cut(s) 99
AfiI CCNNNNNNNGG 6 cut(s) 48, 97, 170, 284, 318, 378
AgeI ACCGGT 1 cut(s) 277
Alw26I GTCTC 4 cut(s) 354, 371, 413, 474
AlwI GGATC 1 cut(s) 151
AoxI GGCC 3 cut(s) 39, 50, 99
ApaI GGGCCC 1 cut(s) 43
AsiGI ACCGGT 1 cut(s) 277
AspLEI GCGC 1 cut(s) 472
AspS9I GGNCC 3 cut(s) 39, 40, 51
AsuHPI GGTGA 2 cut(s) 267, 361
AsuII TTCGAA 1 cut(s) 10
AsuNHI GCTAGC 1 cut(s) 397
BaeGI GKGCMC 1 cut(s) 43
BanII GRGCYC 2 cut(s) 43, 311
BccI CCATC 2 cut(s) 372, 432
BceAI ACGGC 1 cut(s) 193
BcoDI GTCTC 4 cut(s) 354, 371, 413, 474
BfaI CTAG 4 cut(s) 48, 398, 413, 459
BisI GCNGC 2 cut(s) 127, 164
BlpI GCTNAGC 1 cut(s) 473
BlsI GCNGC 2 cut(s) 128, 165
BmgT120I GGNCC 3 cut(s) 39, 40, 51
BmiI GGNNCC 4 cut(s) 41, 53, 125, 310
BmtI GCTAGC 1 cut(s) 401
BplI GAGNNNNNCTC 2 cut(s) 351, 383
Bpu1102I GCTNAGC 1 cut(s) 473
Bpu14I TTCGAA 1 cut(s) 10
BsaI GGTCTC 1 cut(s) 354
BsaJI CCNNGG 2 cut(s) 57, 197
BsaWI WCCGGW 1 cut(s) 277
Bsc4I CCNNNNNNNGG 6 cut(s) 48, 97, 170, 284, 318, 378
Bse118I RCCGGY 2 cut(s) 101, 277
Bse3DI GCAATG 1 cut(s) 191
BseDI CCNNGG 2 cut(s) 57, 197
BseGI GGATG 1 cut(s) 455
BseLI CCNNNNNNNGG 6 cut(s) 48, 97, 170, 284, 318, 378
BseMI GCAATG 1 cut(s) 191
BseSI GKGCMC 1 cut(s) 43
Bsh1236I CGCG 1 cut(s) 166
BshFI GGCC 3 cut(s) 41, 52, 101
BshTI ACCGGT 1 cut(s) 277
BsiSI CCGG 3 cut(s) 102, 255, 278
BslFI GGGAC 2 cut(s) 49, 205
BslI CCNNNNNNNGG 6 cut(s) 48, 97, 170, 284, 318, 378
BsmAI GTCTC 4 cut(s) 354, 371, 413, 474
BsmBI CGTCTC 1 cut(s) 371
BsmFI GGGAC 2 cut(s) 49, 205
BsnI GGCC 3 cut(s) 41, 52, 101
Bso31I GGTCTC 1 cut(s) 354
Bsp119I TTCGAA 1 cut(s) 10
Bsp120I GGGCCC 1 cut(s) 39
Bsp1286I GDGCHC 2 cut(s) 43, 311
Bsp143I GATC 1 cut(s) 143
Bsp1720I GCTNAGC 1 cut(s) 473
BspACI CCGC 9 cut(s) 127, 164, 171, 226, 270, 372, 401, 456, 477
BspANI GGCC 3 cut(s) 41, 52, 101
BspFNI CGCG 1 cut(s) 166
BspLI GGNNCC 4 cut(s) 41, 53, 125, 310
BspOI GCTAGC 1 cut(s) 401
BspPI GGATC 1 cut(s) 151
BspT104I TTCGAA 1 cut(s) 10
BspTNI GGTCTC 1 cut(s) 354
BsrDI GCAATG 1 cut(s) 191
BsrFI RCCGGY 2 cut(s) 101, 277
BssAI RCCGGY 2 cut(s) 101, 277
BssECI CCNNGG 2 cut(s) 57, 197
BssMI GATC 1 cut(s) 143
BssT1I CCWWGG 2 cut(s) 57, 197
Bst4CI ACNGT 2 cut(s) 295, 365
BstBI TTCGAA 1 cut(s) 10
BstC8I GCNNGC 1 cut(s) 399
BstDEI CTNAG 2 cut(s) 353, 473
BstF5I GGATG 1 cut(s) 455
BstFNI CGCG 1 cut(s) 166
BstHHI GCGC 1 cut(s) 472
BstKTI GATC 1 cut(s) 146
BstMAI GTCTC 4 cut(s) 354, 371, 413, 474
BstMBI GATC 1 cut(s) 143
BstMWI GCNNNNNNNGC 1 cut(s) 306
BstNSI RCATGY 1 cut(s) 268
BstSLI GKGCMC 1 cut(s) 43
BstUI CGCG 1 cut(s) 166
BsuRI GGCC 3 cut(s) 41, 52, 101
BtsCI GGATG 1 cut(s) 455
Cac8I GCNNGC 1 cut(s) 399
CfoI GCGC 1 cut(s) 472
Cfr10I RCCGGY 2 cut(s) 101, 277
Cfr13I GGNCC 3 cut(s) 39, 40, 51
CspAI ACCGGT 1 cut(s) 277
CviAII CATG 3 cut(s) 147, 265, 284
CviJI RGCY 6 cut(s) 41, 52, 80, 101, 126, 309
CviKI_1 RGCY 6 cut(s) 41, 52, 80, 101, 126, 309
DdeI CTNAG 2 cut(s) 353, 473
DpnI GATC 1 cut(s) 145
DpnII GATC 1 cut(s) 143
EaeI YGGCCR 1 cut(s) 99
EciI GGCGGA 1 cut(s) 215
Eco130I CCWWGG 2 cut(s) 57, 197
Eco24I GRGCYC 2 cut(s) 43, 311
Eco31I GGTCTC 1 cut(s) 354
Eco32I GATATC 1 cut(s) 233
EcoO109I RGGNCCY 2 cut(s) 40, 51
EcoRV GATATC 1 cut(s) 233
EcoT14I CCWWGG 2 cut(s) 57, 197
EcoT22I ATGCAT 1 cut(s) 266
EcoT38I GRGCYC 2 cut(s) 43, 311
ErhI CCWWGG 2 cut(s) 57, 197
Esp3I CGTCTC 1 cut(s) 371
FaeI CATG 3 cut(s) 150, 268, 287
FaiI YATR 6 cut(s) 25, 86, 148, 266, 285, 324
FaqI GGGAC 2 cut(s) 49, 205
FatI CATG 3 cut(s) 146, 264, 283
Fnu4HI GCNGC 2 cut(s) 127, 164
FokI GGATG 1 cut(s) 462
FriOI GRGCYC 2 cut(s) 43, 311
Fsp4HI GCNGC 2 cut(s) 127, 164
FspBI CTAG 4 cut(s) 48, 398, 413, 459
GlaI GCGC 1 cut(s) 471
GluI GCNGC 2 cut(s) 127, 164
HaeIII GGCC 3 cut(s) 41, 52, 101
HapII CCGG 3 cut(s) 102, 255, 278
HhaI GCGC 1 cut(s) 472
Hin1II CATG 3 cut(s) 150, 268, 287
Hin6I GCGC 1 cut(s) 470
HinP1I GCGC 1 cut(s) 470
HinfI GANTC 2 cut(s) 62, 203
HpaII CCGG 3 cut(s) 102, 255, 278
HphI GGTGA 2 cut(s) 267, 361
HpyAV CCTTC 1 cut(s) 376
HpyCH4III ACNGT 2 cut(s) 295, 365
HpyCH4V TGCA 3 cut(s) 264, 283, 434
HpyF10VI GCNNNNNNNGC 1 cut(s) 306
HpyF3I CTNAG 2 cut(s) 353, 473
Hsp92II CATG 3 cut(s) 150, 268, 287
HspAI GCGC 1 cut(s) 470
Kzo9I GATC 1 cut(s) 143
LmnI GCTCC 2 cut(s) 123, 314
LpnPI CCDG 3 cut(s) 115, 268, 291
MaeI CTAG 4 cut(s) 48, 398, 413, 459
MaeIII GTNAC 2 cut(s) 157, 289
MalI GATC 1 cut(s) 145
MboI GATC 1 cut(s) 143
MhlI GDGCHC 2 cut(s) 43, 311
MlyI GAGTC 1 cut(s) 71
MnlI CCTC 7 cut(s) 53, 101, 112, 133, 181, 233, 312
Mph1103I ATGCAT 1 cut(s) 266
MspI CCGG 3 cut(s) 102, 255, 278
MvnI CGCG 1 cut(s) 166
MwoI GCNNNNNNNGC 1 cut(s) 306
NdeII GATC 1 cut(s) 143
NheI GCTAGC 1 cut(s) 397
NlaIII CATG 3 cut(s) 150, 268, 287
NlaIV GGNNCC 4 cut(s) 41, 53, 125, 310
NmeAIII GCCGAG 1 cut(s) 56
NsiI ATGCAT 1 cut(s) 266
NspI RCATGY 1 cut(s) 268
NspV TTCGAA 1 cut(s) 10
PcsI WCGNNNNNNNCGW 1 cut(s) 212
PfeI GAWTC 1 cut(s) 203
PinAI ACCGGT 1 cut(s) 277
PkrI GCNGC 2 cut(s) 128, 165
PleI GAGTC 1 cut(s) 70
PpsI GAGTC 1 cut(s) 70
PspN4I GGNNCC 4 cut(s) 41, 53, 125, 310
PspOMI GGGCCC 1 cut(s) 39
PspPI GGNCC 3 cut(s) 39, 40, 51
SatI GCNGC 2 cut(s) 127, 164
Sau3AI GATC 1 cut(s) 143
Sau96I GGNCC 3 cut(s) 39, 40, 51
SchI GAGTC 1 cut(s) 71
SduI GDGCHC 2 cut(s) 43, 311
SetI ASST 3 cut(s) 30, 93, 323
SfuI TTCGAA 1 cut(s) 10
SgrAI CRCCGGYG 1 cut(s) 277
SsiI CCGC 9 cut(s) 127, 164, 171, 226, 270, 372, 401, 456, 477
SspMI CTAG 4 cut(s) 48, 398, 413, 459
StyI CCWWGG 2 cut(s) 57, 197
TaaI ACNGT 2 cut(s) 295, 365
TaqI TCGA 3 cut(s) 10, 152, 181
TauI GCSGC 2 cut(s) 129, 166
TfiI GAWTC 1 cut(s) 203
TspGWI ACGGA 4 cut(s) 83, 301, 326, 462
XceI RCATGY 1 cut(s) 268
XspI CTAG 4 cut(s) 48, 398, 413, 459
Zsp2I ATGCAT 1 cut(s) 266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.