Rroxscaffold_5G00366680

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0006965
Physical Location & Seq
Reverse (-)
48660789 .. 48661909
1121 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00366680.1

Sequence Viewer

Length: 414 bp
ATGAAAAGGGAGAAGTTCGCTAGCGGCGATTCTCGACGATTGGTGATCTTCCTTTTCGATGGTTACCTTGTAGAGATGCTTCGTGTGACGGCGTTTGACACGTGGCCAACATGTACTTGGCTTCGCTATGAGATCCGAGAGGAAGATTGCCATGATTTCGTGAAGTTATCGGTCTTTGAGGGACGAAGATCTCCTGCAGCGAAGACAAGTGCCCTCGATCACACCGAAGTTTTCATCTTTGAGATCAAGCCGCTACGGACCATACCCCGGGAAGTAACAATGGGTCGTGCTCCGCGTCCCGAGAGTTTTAGGGCGGGGGAGGATGCCGCTGCCCGCAATAGGCGTGAGGAGAGGTTAGCAAGTCATAGCACTACTAGCGGCTCCGCCGATGGGGGAGAGATCGCGGGGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

137

Amino Acids

15.61

Weight (kDa)

6.33

Isoelectric Point (pI)

54.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 295, 404
AciI CCGC 9 cut(s) 24, 251, 293, 314, 327, 334, 378, 384, 404
AclWI GGATC 1 cut(s) 127
AcoI YGGCCR 1 cut(s) 104
AcvI CACGTG 1 cut(s) 102
AfaI GTAC 1 cut(s) 115
AfiI CCNNNNNNNGG 3 cut(s) 267, 339, 390
AflIII ACRYGT 2 cut(s) 99, 110
Alw21I GWGCWC 1 cut(s) 292
AlwI GGATC 1 cut(s) 127
Ama87I CYCGRG 2 cut(s) 267, 299
AoxI GGCC 1 cut(s) 104
ApeKI GCWGC 2 cut(s) 197, 329
AspS9I GGNCC 1 cut(s) 258
AsuC2I CCSGG 2 cut(s) 268, 269
AsuHPI GGTGA 1 cut(s) 55
AsuNHI GCTAGC 1 cut(s) 20
AvaI CYCGRG 2 cut(s) 267, 299
AvaII GGWCC 1 cut(s) 258
BaeGI GKGCMC 1 cut(s) 214
BalI TGGCCA 1 cut(s) 106
BarI GAAGNNNNNNTAC 2 cut(s) 63, 95
BbrPI CACGTG 1 cut(s) 102
BbsI GAAGAC 1 cut(s) 209
Bbv12I GWGCWC 1 cut(s) 292
BbvI GCAGC 2 cut(s) 209, 316
BccI CCATC 2 cut(s) 53, 383
BceAI ACGGC 1 cut(s) 105
BcnI CCSGG 2 cut(s) 268, 269
BfaI CTAG 2 cut(s) 21, 375
BfmI CTRYAG 1 cut(s) 195
BglII AGATCT 1 cut(s) 188
BisI GCNGC 6 cut(s) 25, 198, 251, 327, 330, 379
BlsI GCNGC 6 cut(s) 26, 199, 252, 328, 331, 380
Bme1390I CCNGG 2 cut(s) 268, 269
Bme18I GGWCC 1 cut(s) 258
BmeT110I CYCGRG 2 cut(s) 267, 299
BmgT120I GGNCC 1 cut(s) 258
BmiI GGNNCC 1 cut(s) 382
BmrFI CCNGG 2 cut(s) 268, 269
BmsI GCATC 2 cut(s) 66, 313
BmtI GCTAGC 1 cut(s) 24
BpiI GAAGAC 1 cut(s) 209
BpuMI CCSGG 2 cut(s) 268, 269
BsaAI YACGTR 1 cut(s) 102
BsaJI CCNNGG 2 cut(s) 266, 267
Bsc4I CCNNNNNNNGG 3 cut(s) 267, 339, 390
BseDI CCNNGG 2 cut(s) 266, 267
BseGI GGATG 1 cut(s) 328
BseLI CCNNNNNNNGG 3 cut(s) 267, 339, 390
BseRI GAGGAG 1 cut(s) 362
BseSI GKGCMC 1 cut(s) 214
BseXI GCAGC 2 cut(s) 209, 316
Bsh1236I CGCG 2 cut(s) 295, 404
BshFI GGCC 1 cut(s) 106
BsiHKAI GWGCWC 1 cut(s) 292
BsiHKCI CYCGRG 2 cut(s) 267, 299
BsiSI CCGG 1 cut(s) 268
BslFI GGGAC 2 cut(s) 195, 282
BslI CCNNNNNNNGG 3 cut(s) 267, 339, 390
BsmFI GGGAC 2 cut(s) 195, 282
BsnI GGCC 1 cut(s) 106
BsoBI CYCGRG 2 cut(s) 267, 299
Bsp1286I GDGCHC 2 cut(s) 214, 292
Bsp143I GATC 6 cut(s) 45, 132, 188, 217, 243, 399
BspACI CCGC 9 cut(s) 24, 251, 293, 314, 327, 334, 378, 384, 404
BspANI GGCC 1 cut(s) 106
BspFNI CGCG 2 cut(s) 295, 404
BspLI GGNNCC 1 cut(s) 382
BspMAI CTGCAG 1 cut(s) 199
BspOI GCTAGC 1 cut(s) 24
BspPI GGATC 1 cut(s) 127
BssECI CCNNGG 2 cut(s) 266, 267
BssMI GATC 6 cut(s) 45, 132, 188, 217, 243, 399
BstBAI YACGTR 1 cut(s) 102
BstC8I GCNNGC 2 cut(s) 22, 334
BstEII GGTNACC 1 cut(s) 62
BstF5I GGATG 1 cut(s) 328
BstFNI CGCG 2 cut(s) 295, 404
BstKTI GATC 6 cut(s) 48, 135, 191, 220, 246, 402
BstMBI GATC 6 cut(s) 45, 132, 188, 217, 243, 399
BstMWI GCNNNNNNNGC 1 cut(s) 375
BstNSI RCATGY 1 cut(s) 114
BstPI GGTNACC 1 cut(s) 62
BstSCI CCNGG 2 cut(s) 266, 267
BstSFI CTRYAG 1 cut(s) 195
BstSLI GKGCMC 1 cut(s) 214
BstUI CGCG 2 cut(s) 295, 404
BstV1I GCAGC 2 cut(s) 209, 316
BstV2I GAAGAC 1 cut(s) 209
BstX2I RGATCY 2 cut(s) 132, 188
BstYI RGATCY 2 cut(s) 132, 188
BsuRI GGCC 1 cut(s) 106
BtsCI GGATG 1 cut(s) 328
Cac8I GCNNGC 2 cut(s) 22, 334
Cfr13I GGNCC 1 cut(s) 258
Cfr9I CCCGGG 1 cut(s) 267
CseI GACGC 1 cut(s) 284
Csp6I GTAC 1 cut(s) 114
CviAII CATG 2 cut(s) 111, 152
CviJI RGCY 4 cut(s) 106, 121, 250, 381
CviKI_1 RGCY 4 cut(s) 106, 121, 250, 381
CviQI GTAC 1 cut(s) 114
DpnI GATC 6 cut(s) 47, 134, 190, 219, 245, 401
DpnII GATC 6 cut(s) 45, 132, 188, 217, 243, 399
EaeI YGGCCR 1 cut(s) 104
EciI GGCGGA 1 cut(s) 373
Eco47I GGWCC 1 cut(s) 258
Eco72I CACGTG 1 cut(s) 102
Eco88I CYCGRG 2 cut(s) 267, 299
Eco91I GGTNACC 1 cut(s) 62
EcoO65I GGTNACC 1 cut(s) 62
FaeI CATG 2 cut(s) 114, 155
FaiI YATR 5 cut(s) 112, 129, 153, 263, 366
FaqI GGGAC 2 cut(s) 195, 282
FatI CATG 2 cut(s) 110, 151
FauI CCCGC 3 cut(s) 307, 341, 397
Fnu4HI GCNGC 6 cut(s) 25, 198, 251, 327, 330, 379
FokI GGATG 1 cut(s) 335
Fsp4HI GCNGC 6 cut(s) 25, 198, 251, 327, 330, 379
FspBI CTAG 2 cut(s) 21, 375
GluI GCNGC 6 cut(s) 25, 198, 251, 327, 330, 379
HaeIII GGCC 1 cut(s) 106
HapII CCGG 1 cut(s) 268
HgaI GACGC 1 cut(s) 284
Hin1II CATG 2 cut(s) 114, 155
HinfI GANTC 1 cut(s) 29
HpaII CCGG 1 cut(s) 268
HphI GGTGA 1 cut(s) 55
Hpy188I TCNGA 1 cut(s) 137
Hpy188III TCNNGA 3 cut(s) 33, 160, 299
Hpy99I CGWCG 1 cut(s) 39
HpyCH4IV ACGT 1 cut(s) 101
HpyCH4V TGCA 1 cut(s) 197
HpyF10VI GCNNNNNNNGC 1 cut(s) 375
HpySE526I ACGT 1 cut(s) 101
Hsp92II CATG 2 cut(s) 114, 155
Kzo9I GATC 6 cut(s) 45, 132, 188, 217, 243, 399
LmnI GCTCC 2 cut(s) 295, 386
LpnPI CCDG 2 cut(s) 207, 281
Lsp1109I GCAGC 2 cut(s) 209, 316
LweI GCATC 2 cut(s) 66, 313
MaeI CTAG 2 cut(s) 21, 375
MaeII ACGT 1 cut(s) 101
MaeIII GTNAC 3 cut(s) 62, 85, 274
MalI GATC 6 cut(s) 47, 134, 190, 219, 245, 401
MboI GATC 6 cut(s) 45, 132, 188, 217, 243, 399
MboII GAAGA 4 cut(s) 40, 155, 198, 214
MflI RGATCY 2 cut(s) 132, 188
MhlI GDGCHC 2 cut(s) 214, 292
MlsI TGGCCA 1 cut(s) 106
MluNI TGGCCA 1 cut(s) 106
MnlI CCTC 6 cut(s) 133, 172, 224, 313, 340, 345
Mox20I TGGCCA 1 cut(s) 106
MscI TGGCCA 1 cut(s) 106
Msp20I TGGCCA 1 cut(s) 106
MspA1I CMGCKG 1 cut(s) 329
MspI CCGG 1 cut(s) 268
MspR9I CCNGG 2 cut(s) 268, 269
MvnI CGCG 2 cut(s) 295, 404
MwoI GCNNNNNNNGC 1 cut(s) 375
NciI CCSGG 2 cut(s) 268, 269
NdeII GATC 6 cut(s) 45, 132, 188, 217, 243, 399
NheI GCTAGC 1 cut(s) 20
NlaIII CATG 2 cut(s) 114, 155
NlaIV GGNNCC 1 cut(s) 382
NmuCI GTSAC 1 cut(s) 85
NspI RCATGY 1 cut(s) 114
PciI ACATGT 1 cut(s) 110
PcsI WCGNNNNNNNCGW 3 cut(s) 24, 222, 292
PfeI GAWTC 1 cut(s) 29
PkrI GCNGC 6 cut(s) 26, 199, 252, 328, 331, 380
PmaCI CACGTG 1 cut(s) 102
PmlI CACGTG 1 cut(s) 102
Ppu21I YACGTR 1 cut(s) 102
PscI ACATGT 1 cut(s) 110
PspCI CACGTG 1 cut(s) 102
PspEI GGTNACC 1 cut(s) 62
PspN4I GGNNCC 1 cut(s) 382
PspPI GGNCC 1 cut(s) 258
PstI CTGCAG 1 cut(s) 199
PsuI RGATCY 2 cut(s) 132, 188
RsaI GTAC 1 cut(s) 115
RsaNI GTAC 1 cut(s) 114
SatI GCNGC 6 cut(s) 25, 198, 251, 327, 330, 379
Sau3AI GATC 6 cut(s) 45, 132, 188, 217, 243, 399
Sau96I GGNCC 1 cut(s) 258
ScrFI CCNGG 2 cut(s) 268, 269
SduI GDGCHC 2 cut(s) 214, 292
SetI ASST 3 cut(s) 69, 104, 356
SfaNI GCATC 2 cut(s) 66, 313
SfcI CTRYAG 1 cut(s) 195
SinI GGWCC 1 cut(s) 258
SmaI CCCGGG 1 cut(s) 269
SsiI CCGC 9 cut(s) 24, 251, 293, 314, 327, 334, 378, 384, 404
SspMI CTAG 2 cut(s) 21, 375
StyD4I CCNGG 2 cut(s) 266, 267
TaiI ACGT 1 cut(s) 104
TaqI TCGA 3 cut(s) 34, 57, 216
TaqII GACCGA 1 cut(s) 160
TatI WGTACW 1 cut(s) 113
TauI GCSGC 4 cut(s) 27, 253, 329, 381
TfiI GAWTC 1 cut(s) 29
TseFI GTSAC 1 cut(s) 85
TseI GCWGC 2 cut(s) 197, 329
Tsp45I GTSAC 1 cut(s) 85
TspDTI ATGAA 2 cut(s) 17, 223
TspGWI ACGGA 1 cut(s) 271
TspMI CCCGGG 1 cut(s) 267
VpaK11BI GGWCC 1 cut(s) 258
XceI RCATGY 1 cut(s) 114
XcmI CCANNNNNNNNNTGG 1 cut(s) 114
XmaI CCCGGG 1 cut(s) 267
XspI CTAG 2 cut(s) 21, 375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.