Rroxscaffold_5G00363750

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0008905
Physical Location & Seq
Reverse (-)
45072206 .. 45075574
3369 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00363750.1

Sequence Viewer

Length: 711 bp
ATGGTGAAGTGTCCGAGGCCAACCAATGTGGTGATCCTCCTCCACGGTCTCTATGCTGGCCTCAAGGCCTTCATCATCGAGGTCGTCGACCGCCGTGCCTCATCTATGGAATCGAGAAGTAGCCGAACAAACAACGGATCGTTGACTCGCGCCCGAACCGGAGCCCGAGCCCGACCTTTCCTCTTATCCTCCGGTGGCCGGTGGCGAGGCCCGGCGACGACAAGCGAGCCTCGTTCGAGGCAAAATCGAAAACCCCTCCTTCGTCTTAGTGATTTCATCTTCAATTTCCGAGTAATGGACTATGACTACCGGAAAACCTCATCCTATGGCGCAAAGATCCCGCCCTACCACTTGTCCTTGTCATCACGGCCGATGTACGGACCTCTGCAGCCGTTGTCGTCGTTTTACTCCAAGTTAACACGATCAAATGTTGGTCGCTCCTCCGTCTCACGGCGGTGGACGTCACCGTCCCTTTCCTCGGGATTGGGCATTCAAGTTTCTATTGAGGCGAGTATCGGATCACTCCTCCGTGATCTATTGAAGATGCAACAAGAAAAGGCGAGATTAGAGCGGAAGCAGCGTGAAGAATTCTTTCCCTTTGTGTTTGCAGTAGTAGATTCCGAAAATCATGTGAATTTGAGTACGAAGCCTCCAATGACCAAGAGATCATCCAGCCAACCAAGAAATGTTGAAATGAAGTCGATGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

236

Amino Acids

26.72

Weight (kDa)

11.23

Isoelectric Point (pI)

65.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 466
AatII GACGTC 1 cut(s) 464
AccBSI CCGCTC 1 cut(s) 571
AccI GTMKAC 1 cut(s) 87
AccII CGCG 1 cut(s) 150
AciI CCGC 4 cut(s) 91, 341, 454, 571
AclWI GGATC 4 cut(s) 28, 145, 331, 526
AcoI YGGCCR 2 cut(s) 196, 368
AcsI RAATTY 2 cut(s) 587, 634
AcyI GRCGYC 1 cut(s) 461
AfaI GTAC 2 cut(s) 377, 643
AfiI CCNNNNNNNGG 5 cut(s) 198, 295, 377, 450, 478
AgsI TTSAA 4 cut(s) 283, 494, 541, 692
AleI CACNNNNGTG 1 cut(s) 454
Alw26I GTCTC 2 cut(s) 53, 451
AlwI GGATC 4 cut(s) 28, 145, 331, 526
Ama87I CYCGRG 2 cut(s) 165, 478
AoxI GGCC 6 cut(s) 17, 58, 66, 196, 208, 368
ApeKI GCWGC 2 cut(s) 388, 577
ApoI RAATTY 2 cut(s) 587, 634
Asp700I GAANNNNTTC 1 cut(s) 591
AspLEI GCGC 2 cut(s) 152, 332
AspS9I GGNCC 2 cut(s) 209, 380
AsuC2I CCSGG 1 cut(s) 212
AsuHPI GGTGA 3 cut(s) 16, 43, 456
AvaI CYCGRG 2 cut(s) 165, 478
AvaII GGWCC 1 cut(s) 380
BanII GRGCYC 2 cut(s) 166, 172
BbvI GCAGC 2 cut(s) 400, 589
BceAI ACGGC 4 cut(s) 78, 376, 383, 467
BcgI CGANNNNNNTGC 2 cut(s) 77, 111
BcnI CCSGG 1 cut(s) 212
BcoDI GTCTC 2 cut(s) 53, 451
BfmI CTRYAG 1 cut(s) 386
BisI GCNGC 2 cut(s) 389, 578
BlsI GCNGC 2 cut(s) 390, 579
Bme1390I CCNGG 1 cut(s) 212
Bme18I GGWCC 1 cut(s) 380
BmeT110I CYCGRG 2 cut(s) 165, 478
BmgT120I GGNCC 2 cut(s) 209, 380
BmiI GGNNCC 1 cut(s) 163
BmrFI CCNGG 1 cut(s) 212
BmsI GCATC 2 cut(s) 534, 693
BpuEI CTTGAG 1 cut(s) 47
BpuMI CCSGG 1 cut(s) 212
BsaHI GRCGYC 1 cut(s) 461
BsaI GGTCTC 1 cut(s) 53
BsaJI CCNNGG 3 cut(s) 14, 43, 477
BsaWI WCCGGW 3 cut(s) 158, 191, 309
BsaXI ACNNNNNCTCC 2 cut(s) 634, 664
Bsc4I CCNNNNNNNGG 5 cut(s) 198, 295, 377, 450, 478
Bse118I RCCGGY 1 cut(s) 198
BseDI CCNNGG 3 cut(s) 14, 43, 477
BseGI GGATG 2 cut(s) 320, 668
BseLI CCNNNNNNNGG 5 cut(s) 198, 295, 377, 450, 478
BseRI GAGGAG 3 cut(s) 29, 430, 515
BseX3I CGGCCG 1 cut(s) 368
BseXI GCAGC 2 cut(s) 400, 589
Bsh1236I CGCG 1 cut(s) 150
Bsh1285I CGRYCG 2 cut(s) 91, 371
BshFI GGCC 6 cut(s) 19, 60, 68, 198, 210, 370
BsiEI CGRYCG 2 cut(s) 91, 371
BsiHKCI CYCGRG 2 cut(s) 165, 478
BsiSI CCGG 5 cut(s) 159, 192, 199, 212, 310
BslFI GGGAC 1 cut(s) 454
BslI CCNNNNNNNGG 5 cut(s) 198, 295, 377, 450, 478
BsmAI GTCTC 2 cut(s) 53, 451
BsmBI CGTCTC 1 cut(s) 451
BsmFI GGGAC 1 cut(s) 454
BsmI GAATGC 1 cut(s) 489
BsnI GGCC 6 cut(s) 19, 60, 68, 198, 210, 370
Bso31I GGTCTC 1 cut(s) 53
BsoBI CYCGRG 2 cut(s) 165, 478
Bsp1286I GDGCHC 2 cut(s) 166, 172
Bsp143I GATC 7 cut(s) 33, 137, 336, 422, 518, 532, 665
BspACI CCGC 4 cut(s) 91, 341, 454, 571
BspANI GGCC 6 cut(s) 19, 60, 68, 198, 210, 370
BspFNI CGCG 1 cut(s) 150
BspLI GGNNCC 1 cut(s) 163
BspMAI CTGCAG 1 cut(s) 390
BspPI GGATC 4 cut(s) 28, 145, 331, 526
BspTNI GGTCTC 1 cut(s) 53
BsrBI CCGCTC 1 cut(s) 571
BsrFI RCCGGY 1 cut(s) 198
BssAI RCCGGY 1 cut(s) 198
BssECI CCNNGG 3 cut(s) 14, 43, 477
BssMI GATC 7 cut(s) 33, 137, 336, 422, 518, 532, 665
BssNI GRCGYC 1 cut(s) 461
Bst4CI ACNGT 2 cut(s) 47, 468
BstACI GRCGYC 1 cut(s) 461
BstC8I GCNNGC 2 cut(s) 58, 227
BstDEI CTNAG 1 cut(s) 266
BstDSI CCRYGG 1 cut(s) 43
BstF5I GGATG 2 cut(s) 320, 668
BstFNI CGCG 1 cut(s) 150
BstHHI GCGC 2 cut(s) 152, 332
BstKTI GATC 7 cut(s) 36, 140, 339, 425, 521, 535, 668
BstMAI GTCTC 2 cut(s) 53, 451
BstMBI GATC 7 cut(s) 33, 137, 336, 422, 518, 532, 665
BstMCI CGRYCG 2 cut(s) 91, 371
BstMWI GCNNNNNNNGC 1 cut(s) 577
BstSCI CCNGG 1 cut(s) 210
BstSFI CTRYAG 1 cut(s) 386
BstUI CGCG 1 cut(s) 150
BstV1I GCAGC 2 cut(s) 400, 589
BstX2I RGATCY 1 cut(s) 336
BstYI RGATCY 1 cut(s) 336
BstZI CGGCCG 1 cut(s) 368
BsuRI GGCC 6 cut(s) 19, 60, 68, 198, 210, 370
BtgI CCRYGG 1 cut(s) 43
BtsCI GGATG 2 cut(s) 320, 668
Cac8I GCNNGC 2 cut(s) 58, 227
CfoI GCGC 2 cut(s) 152, 332
Cfr10I RCCGGY 1 cut(s) 198
Cfr13I GGNCC 2 cut(s) 209, 380
Csp6I GTAC 2 cut(s) 376, 642
CspCI CAANNNNNGTGG 2 cut(s) 9, 44
CviAII CATG 1 cut(s) 629
CviQI GTAC 2 cut(s) 376, 642
DdeI CTNAG 1 cut(s) 266
DpnI GATC 7 cut(s) 35, 139, 338, 424, 520, 534, 667
DpnII GATC 7 cut(s) 33, 137, 336, 422, 518, 532, 665
DrdI GACNNNNNNGTC 1 cut(s) 466
DseDI GACNNNNNNGTC 1 cut(s) 466
EaeI YGGCCR 2 cut(s) 196, 368
EagI CGGCCG 1 cut(s) 368
EclXI CGGCCG 1 cut(s) 368
Eco147I AGGCCT 1 cut(s) 68
Eco24I GRGCYC 2 cut(s) 166, 172
Eco31I GGTCTC 1 cut(s) 53
Eco47I GGWCC 1 cut(s) 380
Eco52I CGGCCG 1 cut(s) 368
Eco88I CYCGRG 2 cut(s) 165, 478
EcoRI GAATTC 1 cut(s) 587
EcoT38I GRGCYC 2 cut(s) 166, 172
Esp3I CGTCTC 1 cut(s) 451
FaeI CATG 1 cut(s) 632
FaiI YATR 5 cut(s) 54, 107, 303, 327, 630
FaqI GGGAC 1 cut(s) 454
FatI CATG 1 cut(s) 628
FauI CCCGC 1 cut(s) 348
FblI GTMKAC 1 cut(s) 87
Fnu4HI GCNGC 2 cut(s) 389, 578
FokI GGATG 2 cut(s) 307, 655
FriOI GRGCYC 2 cut(s) 166, 172
Fsp4HI GCNGC 2 cut(s) 389, 578
GlaI GCGC 2 cut(s) 151, 331
GluI GCNGC 2 cut(s) 389, 578
HaeIII GGCC 6 cut(s) 19, 60, 68, 198, 210, 370
HapII CCGG 5 cut(s) 159, 192, 199, 212, 310
HhaI GCGC 2 cut(s) 152, 332
Hin1I GRCGYC 1 cut(s) 461
Hin1II CATG 1 cut(s) 632
Hin6I GCGC 2 cut(s) 150, 330
HinP1I GCGC 2 cut(s) 150, 330
HincII GTYRAC 3 cut(s) 88, 144, 417
HindII GTYRAC 3 cut(s) 88, 144, 417
HinfI GANTC 3 cut(s) 110, 145, 617
HpaI GTTAAC 1 cut(s) 417
HpaII CCGG 5 cut(s) 159, 192, 199, 212, 310
HphI GGTGA 3 cut(s) 16, 43, 456
Hpy166II GTNNAC 4 cut(s) 88, 144, 417, 459
Hpy188I TCNGA 4 cut(s) 15, 290, 518, 622
Hpy188III TCNNGA 2 cut(s) 114, 480
Hpy8I GTNNAC 4 cut(s) 88, 144, 417, 459
Hpy99I CGWCG 3 cut(s) 89, 220, 403
HpyAV CCTTC 2 cut(s) 79, 269
HpyCH4III ACNGT 2 cut(s) 47, 468
HpyCH4IV ACGT 1 cut(s) 461
HpyCH4V TGCA 3 cut(s) 388, 547, 608
HpyF10VI GCNNNNNNNGC 1 cut(s) 577
HpyF3I CTNAG 1 cut(s) 266
HpySE526I ACGT 1 cut(s) 461
Hsp92I GRCGYC 1 cut(s) 461
Hsp92II CATG 1 cut(s) 632
HspAI GCGC 2 cut(s) 150, 330
KspAI GTTAAC 1 cut(s) 417
Kzo9I GATC 7 cut(s) 33, 137, 336, 422, 518, 532, 665
LmnI GCTCC 2 cut(s) 161, 443
LpnPI CCDG 7 cut(s) 42, 172, 205, 212, 225, 323, 685
Lsp1109I GCAGC 2 cut(s) 400, 589
LweI GCATC 2 cut(s) 534, 693
MaeII ACGT 1 cut(s) 461
MaeIII GTNAC 1 cut(s) 462
MalI GATC 7 cut(s) 35, 139, 338, 424, 520, 534, 667
MbiI CCGCTC 1 cut(s) 571
MboI GATC 7 cut(s) 33, 137, 336, 422, 518, 532, 665
MboII GAAGA 3 cut(s) 271, 553, 596
MflI RGATCY 1 cut(s) 336
MhlI GDGCHC 2 cut(s) 166, 172
MluCI AATT 3 cut(s) 283, 587, 634
MlyI GAGTC 1 cut(s) 139
MroXI GAANNNNTTC 1 cut(s) 591
MseI TTAA 1 cut(s) 416
MslI CAYNNNNRTG 1 cut(s) 454
MspI CCGG 5 cut(s) 159, 192, 199, 212, 310
MspR9I CCNGG 1 cut(s) 212
Mva1269I GAATGC 1 cut(s) 489
MvnI CGCG 1 cut(s) 150
MwoI GCNNNNNNNGC 1 cut(s) 577
NciI CCSGG 1 cut(s) 212
NdeII GATC 7 cut(s) 33, 137, 336, 422, 518, 532, 665
NlaIII CATG 1 cut(s) 632
NlaIV GGNNCC 1 cut(s) 163
NmuCI GTSAC 1 cut(s) 462
OliI CACNNNNGTG 1 cut(s) 454
PceI AGGCCT 1 cut(s) 68
PcsI WCGNNNNNNNCGW 1 cut(s) 84
PctI GAATGC 1 cut(s) 489
PdmI GAANNNNTTC 1 cut(s) 591
PfeI GAWTC 2 cut(s) 110, 617
PkrI GCNGC 2 cut(s) 390, 579
PleI GAGTC 1 cut(s) 139
PpsI GAGTC 1 cut(s) 139
PspN4I GGNNCC 1 cut(s) 163
PspPI GGNCC 2 cut(s) 209, 380
PstI CTGCAG 1 cut(s) 390
PsuI RGATCY 1 cut(s) 336
RsaI GTAC 2 cut(s) 377, 643
RsaNI GTAC 2 cut(s) 376, 642
RseI CAYNNNNRTG 1 cut(s) 454
SalI GTCGAC 1 cut(s) 86
SaqAI TTAA 1 cut(s) 416
SatI GCNGC 2 cut(s) 389, 578
Sau3AI GATC 7 cut(s) 33, 137, 336, 422, 518, 532, 665
Sau96I GGNCC 2 cut(s) 209, 380
SchI GAGTC 1 cut(s) 139
ScrFI CCNGG 1 cut(s) 212
SduI GDGCHC 2 cut(s) 166, 172
SetI ASST 5 cut(s) 84, 178, 320, 385, 464
SfaNI GCATC 2 cut(s) 534, 693
SfcI CTRYAG 1 cut(s) 386
SinI GGWCC 1 cut(s) 380
SmiMI CAYNNNNRTG 1 cut(s) 454
SmlI CTYRAG 1 cut(s) 62
SmoI CTYRAG 1 cut(s) 62
Sse9I AATT 3 cut(s) 283, 587, 634
SseBI AGGCCT 1 cut(s) 68
SsiI CCGC 4 cut(s) 91, 341, 454, 571
StuI AGGCCT 1 cut(s) 68
StyD4I CCNGG 1 cut(s) 210
TaaI ACNGT 2 cut(s) 47, 468
TaiI ACGT 1 cut(s) 464
TaqI TCGA 6 cut(s) 78, 87, 113, 236, 247, 701
TasI AATT 3 cut(s) 283, 587, 634
TfiI GAWTC 2 cut(s) 110, 617
Tru1I TTAA 1 cut(s) 416
Tru9I TTAA 1 cut(s) 416
TseFI GTSAC 1 cut(s) 462
TseI GCWGC 2 cut(s) 388, 577
Tsp45I GTSAC 1 cut(s) 462
TspDTI ATGAA 3 cut(s) 61, 265, 710
TspGWI ACGGA 4 cut(s) 150, 393, 433, 518
VpaK11BI GGWCC 1 cut(s) 380
XapI RAATTY 2 cut(s) 587, 634
XmiI GTMKAC 1 cut(s) 87
XmnI GAANNNNTTC 1 cut(s) 591
ZraI GACGTC 1 cut(s) 462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.