Rorug04G0017900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0008905
Physical Location & Seq
Reverse (-)
2662911 .. 2663894
984 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0017900.1

Sequence Viewer

Length: 579 bp
ATGGCTGCTTCAGCTTCCATGCAATCGATATTCCTAACAAACTCCCTCGCCTATGGAGCTGGAAAGAAGAGCTCAGTCAGAGTAAACCAACTTGTACCAACTCTTCACAGGTACCCTAACATGAGGGTGCGCTCCATGGCTGAGGATGGTCTACCTGAGGAGAAACCATCAAAGATTCCACCGCAAGCACCTACACCTACACCTTTCCCACCACCACCTAGGACTCCCAAGGTTAGCACTAAGTTCGGGGACGTTTTCGCCTTCAGCGGCCCAGCACCTGAAAGAATCAACGGCAGACTCGCAATGGTGGGCTTTGTTTCAGCTCTGGCAGTGGAAGTGTTAAAGGGCCAGGATGTGTTTTCTCAGATCTCCAACGGTGGAGTCTCGCTGTTTCTCGGCACTAGCATTTTGCTATCAGTTGCATCTGTGATTCCTCTATTCCAAGGAGTCAGTGTGGAGTCAAAATCAAAGGGGCCCCTTTGGACCTCAGATGCTGAATTGTGGAATGGAAGACTGGCCATGCTAGGTTTGGTAGCTTTGGCCTTCACTGAGTATGTCAAGGGTGGAACCCTTATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

192

Amino Acids

20.36

Weight (kDa)

9.79

Isoelectric Point (pI)

32.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chloroa_b-bind PF00504 92 - 184 1.1e-09 Chlorophyll A-B binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 111
AccB1I GGYRCC 1 cut(s) 111
AccI GTMKAC 1 cut(s) 151
AciI CCGC 2 cut(s) 182, 267
AcoI YGGCCR 1 cut(s) 516
AcuI CTGAAG 1 cut(s) 247
AfaI GTAC 2 cut(s) 96, 113
AjnI CCWGG 1 cut(s) 348
AluBI AGCT 5 cut(s) 14, 59, 72, 323, 536
AluI AGCT 5 cut(s) 14, 59, 72, 323, 536
Alw21I GWGCWC 1 cut(s) 74
Alw26I GTCTC 1 cut(s) 388
AlwNI CAGNNNCTG 2 cut(s) 278, 494
AoxI GGCC 5 cut(s) 268, 346, 473, 516, 540
ApaI GGGCCC 1 cut(s) 477
ApeKI GCWGC 1 cut(s) 5
Asp718I GGTACC 1 cut(s) 111
AspA2I CCTAGG 1 cut(s) 218
AspLEI GCGC 1 cut(s) 132
AspS9I GGNCC 5 cut(s) 269, 346, 473, 474, 483
AvaII GGWCC 1 cut(s) 483
AvrII CCTAGG 1 cut(s) 218
AxyI CCTNAGG 1 cut(s) 156
BaeGI GKGCMC 1 cut(s) 477
BalI TGGCCA 1 cut(s) 518
BanI GGYRCC 1 cut(s) 111
BanII GRGCYC 2 cut(s) 74, 477
BarI GAAGNNNNNNTAC 2 cut(s) 87, 119
BbsI GAAGAC 1 cut(s) 517
Bbv12I GWGCWC 1 cut(s) 74
BbvCI CCTCAGC 1 cut(s) 141
BccI CCATC 2 cut(s) 140, 175
BceAI ACGGC 1 cut(s) 307
BcgI CGANNNNNNTGC 2 cut(s) 226, 260
BciT130I CCWGG 1 cut(s) 350
BcoDI GTCTC 1 cut(s) 388
BfaI CTAG 3 cut(s) 219, 402, 524
BglII AGATCT 1 cut(s) 366
BisI GCNGC 2 cut(s) 6, 268
BlnI CCTAGG 1 cut(s) 218
BlsI GCNGC 2 cut(s) 7, 269
Bme1390I CCNGG 1 cut(s) 350
Bme18I GGWCC 1 cut(s) 483
BmgT120I GGNCC 5 cut(s) 269, 346, 473, 474, 483
BmiI GGNNCC 5 cut(s) 113, 474, 475, 476, 568
BmrFI CCNGG 1 cut(s) 350
BmsI GCATC 2 cut(s) 431, 481
BpiI GAAGAC 1 cut(s) 517
Bpu10I CCTNAGC 1 cut(s) 141
Bsa29I ATCGAT 1 cut(s) 26
BsaJI CCNNGG 4 cut(s) 135, 218, 228, 442
BsaXI ACNNNNNCTCC 2 cut(s) 438, 468
Bse1I ACTGG 1 cut(s) 519
Bse21I CCTNAGG 1 cut(s) 156
Bse3DI GCAATG 1 cut(s) 309
BseBI CCWGG 1 cut(s) 350
BseCI ATCGAT 1 cut(s) 26
BseDI CCNNGG 4 cut(s) 135, 218, 228, 442
BseGI GGATG 2 cut(s) 151, 358
BseMI GCAATG 1 cut(s) 309
BseMII CTCAG 6 cut(s) 87, 132, 147, 377, 501, 540
BseNI ACTGG 1 cut(s) 519
BseRI GAGGAG 1 cut(s) 173
BseSI GKGCMC 1 cut(s) 477
BseYI CCCAGC 1 cut(s) 271
BshFI GGCC 5 cut(s) 270, 348, 475, 518, 542
BshNI GGYRCC 1 cut(s) 111
BshVI ATCGAT 1 cut(s) 26
BsiHKAI GWGCWC 1 cut(s) 74
BslFI GGGAC 1 cut(s) 263
BsmAI GTCTC 1 cut(s) 388
BsmFI GGGAC 1 cut(s) 263
BsnI GGCC 5 cut(s) 270, 348, 475, 518, 542
Bsp120I GGGCCC 1 cut(s) 473
Bsp1286I GDGCHC 2 cut(s) 74, 477
Bsp143I GATC 1 cut(s) 366
Bsp19I CCATGG 1 cut(s) 135
BspACI CCGC 2 cut(s) 182, 267
BspANI GGCC 5 cut(s) 270, 348, 475, 518, 542
BspCNI CTCAG 6 cut(s) 86, 133, 148, 376, 500, 541
BspDI ATCGAT 1 cut(s) 26
BspLI GGNNCC 5 cut(s) 113, 474, 475, 476, 568
BspQI GCTCTTC 1 cut(s) 62
BspT107I GGYRCC 1 cut(s) 111
BsrDI GCAATG 1 cut(s) 309
BsrI ACTGG 1 cut(s) 519
BssECI CCNNGG 4 cut(s) 135, 218, 228, 442
BssMI GATC 1 cut(s) 366
BssT1I CCWWGG 4 cut(s) 135, 218, 228, 442
Bst2UI CCWGG 1 cut(s) 350
Bst4CI ACNGT 1 cut(s) 377
Bst6I CTCTTC 2 cut(s) 62, 108
BstC8I GCNNGC 1 cut(s) 186
BstDEI CTNAG 7 cut(s) 73, 141, 156, 240, 363, 487, 549
BstDSI CCRYGG 1 cut(s) 135
BstF5I GGATG 2 cut(s) 151, 358
BstHHI GCGC 1 cut(s) 132
BstKTI GATC 1 cut(s) 369
BstMAI GTCTC 1 cut(s) 388
BstMBI GATC 1 cut(s) 366
BstMWI GCNNNNNNNGC 2 cut(s) 11, 56
BstNI CCWGG 1 cut(s) 350
BstSCI CCNGG 1 cut(s) 348
BstSLI GKGCMC 1 cut(s) 477
BstV2I GAAGAC 1 cut(s) 517
BstX2I RGATCY 1 cut(s) 366
BstYI RGATCY 1 cut(s) 366
Bsu15I ATCGAT 1 cut(s) 26
Bsu36I CCTNAGG 1 cut(s) 156
BsuRI GGCC 5 cut(s) 270, 348, 475, 518, 542
BsuTUI ATCGAT 1 cut(s) 26
BtgI CCRYGG 1 cut(s) 135
BtsCI GGATG 2 cut(s) 151, 358
BtsI GCAGTG 1 cut(s) 336
BtsIMutI CAGTG 3 cut(s) 336, 457, 546
Cac8I GCNNGC 1 cut(s) 186
CaiI CAGNNNCTG 2 cut(s) 278, 494
CfoI GCGC 1 cut(s) 132
Cfr13I GGNCC 5 cut(s) 269, 346, 473, 474, 483
ClaI ATCGAT 1 cut(s) 26
Csp6I GTAC 2 cut(s) 95, 112
CviAII CATG 4 cut(s) 19, 121, 136, 520
CviQI GTAC 2 cut(s) 95, 112
DdeI CTNAG 7 cut(s) 73, 141, 156, 240, 363, 487, 549
DpnI GATC 1 cut(s) 368
DpnII GATC 1 cut(s) 366
EaeI YGGCCR 1 cut(s) 516
Eam1104I CTCTTC 2 cut(s) 62, 108
EarI CTCTTC 2 cut(s) 62, 108
Ecl136II GAGCTC 1 cut(s) 72
Eco130I CCWWGG 4 cut(s) 135, 218, 228, 442
Eco24I GRGCYC 2 cut(s) 74, 477
Eco47I GGWCC 1 cut(s) 483
Eco53kI GAGCTC 1 cut(s) 72
Eco57I CTGAAG 1 cut(s) 247
Eco81I CCTNAGG 1 cut(s) 156
EcoICRI GAGCTC 1 cut(s) 72
EcoO109I RGGNCCY 2 cut(s) 473, 474
EcoRII CCWGG 1 cut(s) 348
EcoT14I CCWWGG 4 cut(s) 135, 218, 228, 442
EcoT38I GRGCYC 2 cut(s) 74, 477
ErhI CCWWGG 4 cut(s) 135, 218, 228, 442
FaeI CATG 4 cut(s) 22, 124, 139, 523
FaiI YATR 6 cut(s) 20, 54, 122, 137, 521, 555
FaqI GGGAC 1 cut(s) 263
FatI CATG 4 cut(s) 18, 120, 135, 519
FblI GTMKAC 1 cut(s) 151
Fnu4HI GCNGC 2 cut(s) 6, 268
FokI GGATG 2 cut(s) 158, 365
FriOI GRGCYC 2 cut(s) 74, 477
Fsp4HI GCNGC 2 cut(s) 6, 268
FspBI CTAG 3 cut(s) 219, 402, 524
GlaI GCGC 1 cut(s) 131
GluI GCNGC 2 cut(s) 6, 268
GsaI CCCAGC 1 cut(s) 275
HaeIII GGCC 5 cut(s) 270, 348, 475, 518, 542
HhaI GCGC 1 cut(s) 132
Hin1II CATG 4 cut(s) 22, 124, 139, 523
Hin6I GCGC 1 cut(s) 130
HinP1I GCGC 1 cut(s) 130
HinfI GANTC 8 cut(s) 175, 223, 285, 297, 381, 430, 447, 458
Hpy166II GTNNAC 2 cut(s) 85, 152
Hpy188I TCNGA 3 cut(s) 80, 366, 490
Hpy8I GTNNAC 2 cut(s) 85, 152
HpyAV CCTTC 2 cut(s) 271, 553
HpyCH4III ACNGT 1 cut(s) 377
HpyCH4IV ACGT 1 cut(s) 252
HpyCH4V TGCA 2 cut(s) 22, 422
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 56
HpyF3I CTNAG 7 cut(s) 73, 141, 156, 240, 363, 487, 549
HpySE526I ACGT 1 cut(s) 252
Hsp92II CATG 4 cut(s) 22, 124, 139, 523
HspAI GCGC 1 cut(s) 130
KpnI GGTACC 1 cut(s) 115
Kzo9I GATC 1 cut(s) 366
LguI GCTCTTC 1 cut(s) 62
LmnI GCTCC 2 cut(s) 56, 137
LpnPI CCDG 9 cut(s) 45, 94, 168, 285, 291, 311, 335, 362, 500
LweI GCATC 2 cut(s) 431, 481
MaeI CTAG 3 cut(s) 219, 402, 524
MaeII ACGT 1 cut(s) 252
MalI GATC 1 cut(s) 368
MboI GATC 1 cut(s) 366
MboII GAAGA 3 cut(s) 79, 95, 522
MflI RGATCY 1 cut(s) 366
MhlI GDGCHC 2 cut(s) 74, 477
MlsI TGGCCA 1 cut(s) 518
MluCI AATT 1 cut(s) 497
MluNI TGGCCA 1 cut(s) 518
MlyI GAGTC 5 cut(s) 217, 291, 390, 456, 467
MmeI TCCRAC 1 cut(s) 396
MnlI CCTC 6 cut(s) 56, 117, 136, 151, 444, 496
Mox20I TGGCCA 1 cut(s) 518
MscI TGGCCA 1 cut(s) 518
MseI TTAA 2 cut(s) 341, 577
MslI CAYNNNNRTG 1 cut(s) 125
Msp20I TGGCCA 1 cut(s) 518
MspA1I CMGCKG 1 cut(s) 267
MspR9I CCNGG 1 cut(s) 350
MvaI CCWGG 1 cut(s) 350
MwoI GCNNNNNNNGC 2 cut(s) 11, 56
NcoI CCATGG 1 cut(s) 135
NdeII GATC 1 cut(s) 366
NlaIII CATG 4 cut(s) 22, 124, 139, 523
NlaIV GGNNCC 5 cut(s) 113, 474, 475, 476, 568
NmeAIII GCCGAG 1 cut(s) 375
PciSI GCTCTTC 1 cut(s) 62
PfeI GAWTC 3 cut(s) 175, 285, 430
PkrI GCNGC 2 cut(s) 7, 269
PleI GAGTC 5 cut(s) 217, 291, 389, 455, 466
PpsI GAGTC 5 cut(s) 217, 291, 389, 455, 466
Psp124BI GAGCTC 1 cut(s) 74
Psp6I CCWGG 1 cut(s) 348
PspFI CCCAGC 1 cut(s) 271
PspGI CCWGG 1 cut(s) 348
PspN4I GGNNCC 5 cut(s) 113, 474, 475, 476, 568
PspOMI GGGCCC 1 cut(s) 473
PspPI GGNCC 5 cut(s) 269, 346, 473, 474, 483
PstNI CAGNNNCTG 2 cut(s) 278, 494
PsuI RGATCY 1 cut(s) 366
RsaI GTAC 2 cut(s) 96, 113
RsaNI GTAC 2 cut(s) 95, 112
RseI CAYNNNNRTG 1 cut(s) 125
SacI GAGCTC 1 cut(s) 74
SapI GCTCTTC 1 cut(s) 62
SaqAI TTAA 2 cut(s) 341, 577
SatI GCNGC 2 cut(s) 6, 268
Sau3AI GATC 1 cut(s) 366
Sau96I GGNCC 5 cut(s) 269, 346, 473, 474, 483
SchI GAGTC 5 cut(s) 217, 291, 390, 456, 467
ScrFI CCNGG 1 cut(s) 350
SduI GDGCHC 2 cut(s) 74, 477
SfaNI GCATC 2 cut(s) 431, 481
SinI GGWCC 1 cut(s) 483
SmiMI CAYNNNNRTG 1 cut(s) 125
Sse9I AATT 1 cut(s) 497
SsiI CCGC 2 cut(s) 182, 267
SspMI CTAG 3 cut(s) 219, 402, 524
SstI GAGCTC 1 cut(s) 74
StyD4I CCNGG 1 cut(s) 348
StyI CCWWGG 4 cut(s) 135, 218, 228, 442
TaaI ACNGT 1 cut(s) 377
TaiI ACGT 1 cut(s) 255
TaqI TCGA 1 cut(s) 26
TasI AATT 1 cut(s) 497
TauI GCSGC 1 cut(s) 270
TfiI GAWTC 3 cut(s) 175, 285, 430
Tru1I TTAA 2 cut(s) 341, 577
Tru9I TTAA 2 cut(s) 341, 577
TscAI CASTG 3 cut(s) 336, 457, 553
TseI GCWGC 1 cut(s) 5
TspRI CASTG 3 cut(s) 336, 457, 553
VpaK11BI GGWCC 1 cut(s) 483
XcmI CCANNNNNNNNNTGG 1 cut(s) 526
XmaJI CCTAGG 1 cut(s) 218
XmiI GTMKAC 1 cut(s) 151
XspI CTAG 3 cut(s) 219, 402, 524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.