Rroxscaffold_5G00374840

palmitoyl-(protein) hydrolase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0007162
Physical Location & Seq
Forward (+)
55788579 .. 55791779
3201 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00374840.1

Sequence Viewer

Length: 1407 bp
ATGTACGCTAGTGTGAGGAAGATGATCAGATTATGGATAGCCGCACTGCAATTTTCGGAGCTGTTTGTGAGCACCATGGTTCATTTGCTTTATGGGTTTTACATATTCAGCTCCGCCGTCGCCGGTGACCTCTCGCTGGCCTTGAATGAGCTCTTGTGTAAGCCCAACAGCGTCACGAAAGAGTTGGTGCCCAGTGAGACCTCGGCGGCCGCGCCCAATGGGGCTCTGCCTCCTATTGTTTTGGTCCATGGGATTTTTGGCTTTGGCAAAGGGAGGCTGGGAGGTTTGTCGTATTTCGCCGGAGCTGAGAAGAAAGACGAGAGGGTTCTGGTGCCGGATTTGGGATCTCTGACCAGCGTTTACGACAGGGCTCGTGAGTTGTTCTACTATTTGAAAGGTGGGCGGGTCGATTATGGTGAAGAACACAGCAAAGCCTGTGGGCATTCCCAGTTTGGCCGTCTTTATGAACAAGGGCACTACCCTGAATGGGATGAGGATCATCCTATTCACTTTGTGGGCCATTCCGCCGGAGCTCAGGTCGTCCGTGTTCTGCAGCAAATGCTTGCTGATAAGGCTTTCAAGGGGTATGAGCATACTAATGAGAATTGGGTATTGAGCATCACATCATTGTCCGGAGCGTTCAATGGGACTACAAGAACTTACTTGGATGGAATGCAGCCTGAGGATGGCAAAACCCTGAAGCCCATATGTCTGCTGCAACTATGTCGCATAGGAGTGATTATTTATGACTGGCTAGACATTTCCTGGCTAAAGGAATATTACAACTTTGGGTTTGATCACTATAATATCACATGGAAAAAAGTCGGTGTTTGGGGTCTCATTGATTGCCTCTTGGGAAATTCGGGACCATTCGCTTCTGGGGACTGGATCCTCCCTGACCTTACAATTCAAGGTTCCATAAGACTCAACAGCCATCTACAGTCCTTTCCAAATACATACTACTTCAGCTATGCCACAAAGCGCACTAGGAAGATATGGGGTGTCACACTTCCTTCTAGCATATTCGGAATTCACCCTCTGCTTTTCATCAGAGTACTGCAGATGAGCCAATGGCGTCATCCTCCTGATGTCTCTCCCCCATATAAGGGTTACAGAGATGAGGATTGGCAAGAAAATGATGGAGCTCTCAATACCATCTCCATGACTCGCCCTCTTCTTCCTGTTGAACACCCAGGCCGTTTTGTGAAAGATGATTCAGAGTGCCACCCCTTGCAACCAGGCATCTGGTACTACAAGATTGTGGAAGCTGATCACATATTCTTCATTGTGAATAGGGAGAGAGCAGGAGTTCAATTTGATCTGATATATGACAGCATTTTTGAAAGGTGCAGAAAGCATGTATTTAGAAAAACTCCACCAACATTACCCAACCAAACGCACCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

468

Amino Acids

53.32

Weight (kDa)

6.83

Isoelectric Point (pI)

35.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lip_C PF24708 78 - 190 3e-20 Lipase-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 187, 331
AccII CGCG 1 cut(s) 212
AccIII TCCGGA 1 cut(s) 632
AciI CCGC 6 cut(s) 42, 114, 206, 210, 403, 525
AclWI GGATC 4 cut(s) 352, 504, 883, 896
AcoI YGGCCR 2 cut(s) 207, 454
AcsI RAATTY 2 cut(s) 859, 1029
AcuI CTGAAG 2 cut(s) 719, 949
AcyI GRCGYC 1 cut(s) 1075
AdeI CACNNNGTG 1 cut(s) 514
AfaI GTAC 3 cut(s) 5, 1056, 1250
AfiI CCNNNNNNNGG 6 cut(s) 136, 341, 487, 686, 1105, 1106
AgsI TTSAA 8 cut(s) 145, 394, 580, 643, 911, 1187, 1313, 1343
AjnI CCWGG 3 cut(s) 764, 1192, 1237
AluBI AGCT 8 cut(s) 61, 111, 151, 305, 533, 969, 1145, 1268
AluI AGCT 8 cut(s) 61, 111, 151, 305, 533, 969, 1145, 1268
Alw21I GWGCWC 4 cut(s) 74, 153, 535, 1147
Alw26I GTCTC 3 cut(s) 191, 842, 1096
AlwI GGATC 4 cut(s) 352, 504, 883, 896
Aor13HI TCCGGA 1 cut(s) 632
AoxI GGCC 5 cut(s) 138, 207, 454, 517, 1195
ApeKI GCWGC 3 cut(s) 553, 676, 715
ApoI RAATTY 2 cut(s) 859, 1029
ArsI GACNNNNNNTTYG 2 cut(s) 1322, 1354
AspLEI GCGC 2 cut(s) 214, 984
AspS9I GGNCC 3 cut(s) 244, 517, 866
AsuHPI GGTGA 3 cut(s) 137, 428, 1025
AvaII GGWCC 2 cut(s) 244, 866
AxyI CCTNAGG 1 cut(s) 681
BaeGI GKGCMC 2 cut(s) 192, 477
BamHI GGATCC 1 cut(s) 888
BanI GGYRCC 2 cut(s) 187, 331
BanII GRGCYC 5 cut(s) 153, 226, 373, 535, 1147
BauI CACGAG 1 cut(s) 372
Bbv12I GWGCWC 4 cut(s) 74, 153, 535, 1147
BbvI GCAGC 3 cut(s) 565, 688, 702
BccI CCATC 5 cut(s) 662, 680, 942, 1133, 1163
BceAI ACGGC 3 cut(s) 101, 441, 1182
BcgI CGANNNNNNTGC 2 cut(s) 707, 741
BciT130I CCWGG 3 cut(s) 766, 1194, 1239
BclI TGATCA 3 cut(s) 24, 796, 1270
BcoDI GTCTC 3 cut(s) 191, 842, 1096
BfaI CTAG 4 cut(s) 9, 755, 987, 1017
BfmI CTRYAG 3 cut(s) 551, 938, 1058
BisI GCNGC 6 cut(s) 42, 207, 210, 554, 677, 716
BlsI GCNGC 6 cut(s) 43, 208, 211, 555, 678, 717
BmcAI AGTACT 1 cut(s) 1056
Bme1390I CCNGG 3 cut(s) 766, 1194, 1239
Bme18I GGWCC 2 cut(s) 244, 866
BmgT120I GGNCC 3 cut(s) 244, 517, 866
BmiI GGNNCC 5 cut(s) 189, 333, 867, 890, 916
BmrFI CCNGG 3 cut(s) 766, 1194, 1239
BmrI ACTGGG 2 cut(s) 186, 442
BmsI GCATC 2 cut(s) 627, 1251
BmuI ACTGGG 2 cut(s) 186, 442
Bpu10I CCTNAGC 1 cut(s) 534
BsaBI GATNNNNATC 1 cut(s) 495
BsaHI GRCGYC 1 cut(s) 1075
BsaI GGTCTC 2 cut(s) 191, 842
BsaJI CCNNGG 4 cut(s) 75, 201, 247, 1192
BsaWI WCCGGW 1 cut(s) 632
BsaXI ACNNNNNCTCC 2 cut(s) 522, 552
Bsc4I CCNNNNNNNGG 6 cut(s) 136, 341, 487, 686, 1105, 1106
Bse118I RCCGGY 1 cut(s) 122
Bse1I ACTGG 4 cut(s) 192, 448, 755, 890
Bse21I CCTNAGG 1 cut(s) 681
Bse8I GATNNNNATC 1 cut(s) 495
BseAI TCCGGA 1 cut(s) 632
BseBI CCWGG 3 cut(s) 766, 1194, 1239
BseDI CCNNGG 4 cut(s) 75, 201, 247, 1192
BseGI GGATG 5 cut(s) 496, 499, 673, 691, 1078
BseJI GATNNNNATC 1 cut(s) 495
BseLI CCNNNNNNNGG 6 cut(s) 136, 341, 487, 686, 1105, 1106
BseMII CTCAG 3 cut(s) 297, 548, 672
BseNI ACTGG 4 cut(s) 192, 448, 755, 890
BseSI GKGCMC 2 cut(s) 192, 477
BseX3I CGGCCG 1 cut(s) 207
BseXI GCAGC 3 cut(s) 565, 688, 702
BseYI CCCAGC 1 cut(s) 277
BsgI GTGCAG 1 cut(s) 1369
Bsh1236I CGCG 1 cut(s) 212
Bsh1285I CGRYCG 1 cut(s) 210
BshFI GGCC 5 cut(s) 140, 209, 456, 519, 1197
BshNI GGYRCC 2 cut(s) 187, 331
BsiEI CGRYCG 1 cut(s) 210
BsiHKAI GWGCWC 4 cut(s) 74, 153, 535, 1147
BsiSI CCGG 5 cut(s) 123, 300, 335, 528, 633
BslFI GGGAC 3 cut(s) 661, 879, 896
BslI CCNNNNNNNGG 6 cut(s) 136, 341, 487, 686, 1105, 1106
BsmAI GTCTC 3 cut(s) 191, 842, 1096
BsmFI GGGAC 3 cut(s) 661, 879, 896
BsmI GAATGC 2 cut(s) 442, 678
BsnI GGCC 5 cut(s) 140, 209, 456, 519, 1197
Bso31I GGTCTC 2 cut(s) 191, 842
Bsp1286I GDGCHC 8 cut(s) 74, 153, 192, 226, 373, 477, 535, 1147
Bsp13I TCCGGA 1 cut(s) 632
Bsp143I GATC 7 cut(s) 24, 344, 496, 796, 888, 1270, 1318
Bsp19I CCATGG 2 cut(s) 75, 247
BspACI CCGC 6 cut(s) 42, 114, 206, 210, 403, 525
BspANI GGCC 5 cut(s) 140, 209, 456, 519, 1197
BspCNI CTCAG 3 cut(s) 298, 547, 673
BspEI TCCGGA 1 cut(s) 632
BspFNI CGCG 1 cut(s) 212
BspLI GGNNCC 5 cut(s) 189, 333, 867, 890, 916
BspMAI CTGCAG 2 cut(s) 555, 1062
BspPI GGATC 4 cut(s) 352, 504, 883, 896
BspT107I GGYRCC 2 cut(s) 187, 331
BspTNI GGTCTC 2 cut(s) 191, 842
BsrFI RCCGGY 1 cut(s) 122
BsrI ACTGG 4 cut(s) 192, 448, 755, 890
BssAI RCCGGY 1 cut(s) 122
BssECI CCNNGG 4 cut(s) 75, 201, 247, 1192
BssMI GATC 7 cut(s) 24, 344, 496, 796, 888, 1270, 1318
BssNI GRCGYC 1 cut(s) 1075
BssSI CACGAG 1 cut(s) 372
BssT1I CCWWGG 2 cut(s) 75, 247
Bst2BI CACGAG 1 cut(s) 372
Bst2UI CCWGG 3 cut(s) 766, 1194, 1239
Bst4CI ACNGT 1 cut(s) 942
Bst6I CTCTTC 1 cut(s) 1179
BstACI GRCGYC 1 cut(s) 1075
BstAPI GCANNNNNTGC 1 cut(s) 559
BstC8I GCNNGC 2 cut(s) 138, 564
BstDEI CTNAG 3 cut(s) 306, 534, 681
BstDSI CCRYGG 2 cut(s) 75, 247
BstEII GGTNACC 1 cut(s) 125
BstF5I GGATG 5 cut(s) 496, 499, 673, 691, 1078
BstFNI CGCG 1 cut(s) 212
BstHHI GCGC 2 cut(s) 214, 984
BstKTI GATC 7 cut(s) 27, 347, 499, 799, 891, 1273, 1321
BstMAI GTCTC 3 cut(s) 191, 842, 1096
BstMBI GATC 7 cut(s) 24, 344, 496, 796, 888, 1270, 1318
BstMCI CGRYCG 1 cut(s) 210
BstMWI GCNNNNNNNGC 2 cut(s) 559, 572
BstNI CCWGG 3 cut(s) 766, 1194, 1239
BstNSI RCATGY 1 cut(s) 1361
BstPI GGTNACC 1 cut(s) 125
BstSCI CCNGG 3 cut(s) 764, 1192, 1237
BstSFI CTRYAG 3 cut(s) 551, 938, 1058
BstSLI GKGCMC 2 cut(s) 192, 477
BstUI CGCG 1 cut(s) 212
BstV1I GCAGC 3 cut(s) 565, 688, 702
BstX2I RGATCY 2 cut(s) 344, 888
BstXI CCANNNNNNTGG 1 cut(s) 1245
BstYI RGATCY 2 cut(s) 344, 888
BstZI CGGCCG 1 cut(s) 207
Bsu36I CCTNAGG 1 cut(s) 681
BsuRI GGCC 5 cut(s) 140, 209, 456, 519, 1197
BtgI CCRYGG 2 cut(s) 75, 247
BtsCI GGATG 5 cut(s) 496, 499, 673, 691, 1078
BtsI GCAGTG 1 cut(s) 44
BtsIMutI CAGTG 2 cut(s) 44, 199
Cac8I GCNNGC 2 cut(s) 138, 564
CciNI GCGGCCGC 1 cut(s) 207
CfoI GCGC 2 cut(s) 214, 984
Cfr10I RCCGGY 1 cut(s) 122
Cfr13I GGNCC 3 cut(s) 244, 517, 866
CseI GACGC 2 cut(s) 160, 1064
Csp6I GTAC 3 cut(s) 4, 1055, 1249
CspCI CAANNNNNGTGG 2 cut(s) 418, 453
CviAII CATG 5 cut(s) 76, 248, 813, 1162, 1358
CviQI GTAC 3 cut(s) 4, 1055, 1249
DdeI CTNAG 3 cut(s) 306, 534, 681
DpnI GATC 7 cut(s) 26, 346, 498, 798, 890, 1272, 1320
DpnII GATC 7 cut(s) 24, 344, 496, 796, 888, 1270, 1318
DraIII CACNNNGTG 1 cut(s) 514
EaeI YGGCCR 2 cut(s) 207, 454
EagI CGGCCG 1 cut(s) 207
Eam1104I CTCTTC 1 cut(s) 1179
EarI CTCTTC 1 cut(s) 1179
EciI GGCGGA 2 cut(s) 103, 514
Ecl136II GAGCTC 3 cut(s) 151, 533, 1145
EclXI CGGCCG 1 cut(s) 207
Eco130I CCWWGG 2 cut(s) 75, 247
Eco24I GRGCYC 5 cut(s) 153, 226, 373, 535, 1147
Eco31I GGTCTC 2 cut(s) 191, 842
Eco47I GGWCC 2 cut(s) 244, 866
Eco52I CGGCCG 1 cut(s) 207
Eco53kI GAGCTC 3 cut(s) 151, 533, 1145
Eco57I CTGAAG 2 cut(s) 719, 949
Eco81I CCTNAGG 1 cut(s) 681
Eco91I GGTNACC 1 cut(s) 125
EcoICRI GAGCTC 3 cut(s) 151, 533, 1145
EcoO65I GGTNACC 1 cut(s) 125
EcoRI GAATTC 1 cut(s) 1029
EcoRII CCWGG 3 cut(s) 764, 1192, 1237
EcoT14I CCWWGG 2 cut(s) 75, 247
EcoT38I GRGCYC 5 cut(s) 153, 226, 373, 535, 1147
ErhI CCWWGG 2 cut(s) 75, 247
FaeI CATG 5 cut(s) 79, 251, 816, 1165, 1361
FaqI GGGAC 3 cut(s) 661, 879, 896
FatI CATG 5 cut(s) 75, 247, 812, 1161, 1357
FauI CCCGC 1 cut(s) 396
FauNDI CATATG 1 cut(s) 707
FbaI TGATCA 3 cut(s) 24, 796, 1270
Fnu4HI GCNGC 6 cut(s) 42, 207, 210, 554, 677, 716
FokI GGATG 5 cut(s) 486, 503, 680, 698, 1065
FriOI GRGCYC 5 cut(s) 153, 226, 373, 535, 1147
Fsp4HI GCNGC 6 cut(s) 42, 207, 210, 554, 677, 716
FspBI CTAG 4 cut(s) 9, 755, 987, 1017
GlaI GCGC 2 cut(s) 213, 983
GluI GCNGC 6 cut(s) 42, 207, 210, 554, 677, 716
GsaI CCCAGC 1 cut(s) 281
HaeIII GGCC 5 cut(s) 140, 209, 456, 519, 1197
HapII CCGG 5 cut(s) 123, 300, 335, 528, 633
HgaI GACGC 2 cut(s) 160, 1064
HhaI GCGC 2 cut(s) 214, 984
Hin1I GRCGYC 1 cut(s) 1075
Hin1II CATG 5 cut(s) 79, 251, 816, 1165, 1361
Hin6I GCGC 2 cut(s) 212, 982
HinP1I GCGC 2 cut(s) 212, 982
HinfI GANTC 3 cut(s) 924, 1165, 1214
HpaII CCGG 5 cut(s) 123, 300, 335, 528, 633
HphI GGTGA 3 cut(s) 137, 428, 1025
Hpy166II GTNNAC 1 cut(s) 361
Hpy188I TCNGA 7 cut(s) 29, 58, 351, 1028, 1052, 1219, 1323
Hpy188III TCNNGA 5 cut(s) 175, 374, 633, 864, 1085
Hpy8I GTNNAC 1 cut(s) 361
Hpy99I CGWCG 1 cut(s) 122
HpyAV CCTTC 1 cut(s) 1023
HpyCH4III ACNGT 1 cut(s) 942
HpyCH4V TGCA 7 cut(s) 49, 553, 676, 718, 1060, 1234, 1350
HpyF10VI GCNNNNNNNGC 2 cut(s) 559, 572
HpyF3I CTNAG 3 cut(s) 306, 534, 681
Hsp92I GRCGYC 1 cut(s) 1075
Hsp92II CATG 5 cut(s) 79, 251, 816, 1165, 1361
HspAI GCGC 2 cut(s) 212, 982
Kpn2I TCCGGA 1 cut(s) 632
Ksp22I TGATCA 3 cut(s) 24, 796, 1270
Kzo9I GATC 7 cut(s) 24, 344, 496, 796, 888, 1270, 1318
LmnI GCTCC 6 cut(s) 58, 116, 302, 530, 635, 1142
Lsp1109I GCAGC 3 cut(s) 565, 688, 702
LweI GCATC 2 cut(s) 627, 1251
MaeI CTAG 4 cut(s) 9, 755, 987, 1017
MaeIII GTNAC 4 cut(s) 125, 172, 1003, 1109
MalI GATC 7 cut(s) 26, 346, 498, 798, 890, 1272, 1320
MboI GATC 7 cut(s) 24, 344, 496, 796, 888, 1270, 1318
MboII GAAGA 7 cut(s) 31, 322, 431, 1003, 1166, 1169, 1273
MflI RGATCY 2 cut(s) 344, 888
MhlI GDGCHC 8 cut(s) 74, 153, 192, 226, 373, 477, 535, 1147
MluCI AATT 6 cut(s) 50, 604, 859, 906, 1029, 1313
MlyI GAGTC 2 cut(s) 918, 1159
MroI TCCGGA 1 cut(s) 632
MslI CAYNNNNRTG 3 cut(s) 597, 734, 1160
MspI CCGG 5 cut(s) 123, 300, 335, 528, 633
MspR9I CCNGG 3 cut(s) 766, 1194, 1239
Mva1269I GAATGC 2 cut(s) 442, 678
MvaI CCWGG 3 cut(s) 766, 1194, 1239
MvnI CGCG 1 cut(s) 212
MwoI GCNNNNNNNGC 2 cut(s) 559, 572
NcoI CCATGG 2 cut(s) 75, 247
NdeI CATATG 1 cut(s) 707
NdeII GATC 7 cut(s) 24, 344, 496, 796, 888, 1270, 1318
NlaIII CATG 5 cut(s) 79, 251, 816, 1165, 1361
NlaIV GGNNCC 5 cut(s) 189, 333, 867, 890, 916
NmeAIII GCCGAG 1 cut(s) 182
NmuCI GTSAC 3 cut(s) 125, 172, 1003
NotI GCGGCCGC 1 cut(s) 207
NspI RCATGY 1 cut(s) 1361
PctI GAATGC 2 cut(s) 442, 678
PfeI GAWTC 1 cut(s) 1214
PkrI GCNGC 6 cut(s) 43, 208, 211, 555, 678, 717
PleI GAGTC 2 cut(s) 918, 1159
PpsI GAGTC 2 cut(s) 918, 1159
Psp124BI GAGCTC 3 cut(s) 153, 535, 1147
Psp6I CCWGG 3 cut(s) 764, 1192, 1237
PspEI GGTNACC 1 cut(s) 125
PspFI CCCAGC 1 cut(s) 277
PspGI CCWGG 3 cut(s) 764, 1192, 1237
PspN4I GGNNCC 5 cut(s) 189, 333, 867, 890, 916
PspPI GGNCC 3 cut(s) 244, 517, 866
PstI CTGCAG 2 cut(s) 555, 1062
PsuI RGATCY 2 cut(s) 344, 888
RsaI GTAC 3 cut(s) 5, 1056, 1250
RsaNI GTAC 3 cut(s) 4, 1055, 1249
RseI CAYNNNNRTG 3 cut(s) 597, 734, 1160
SacI GAGCTC 3 cut(s) 153, 535, 1147
SatI GCNGC 6 cut(s) 42, 207, 210, 554, 677, 716
Sau3AI GATC 7 cut(s) 24, 344, 496, 796, 888, 1270, 1318
Sau96I GGNCC 3 cut(s) 244, 517, 866
ScaI AGTACT 1 cut(s) 1056
SchI GAGTC 2 cut(s) 918, 1159
ScrFI CCNGG 3 cut(s) 766, 1194, 1239
SduI GDGCHC 8 cut(s) 74, 153, 192, 226, 373, 477, 535, 1147
SfaNI GCATC 2 cut(s) 627, 1251
SfcI CTRYAG 3 cut(s) 551, 938, 1058
SgrAI CRCCGGYG 1 cut(s) 122
SinI GGWCC 2 cut(s) 244, 866
SmiMI CAYNNNNRTG 3 cut(s) 597, 734, 1160
Sse9I AATT 6 cut(s) 50, 604, 859, 906, 1029, 1313
SsiI CCGC 6 cut(s) 42, 114, 206, 210, 403, 525
SspI AATATT 1 cut(s) 779
SspMI CTAG 4 cut(s) 9, 755, 987, 1017
SstI GAGCTC 3 cut(s) 153, 535, 1147
StyD4I CCNGG 3 cut(s) 764, 1192, 1237
StyI CCWWGG 2 cut(s) 75, 247
TaaI ACNGT 1 cut(s) 942
TaqI TCGA 1 cut(s) 408
TasI AATT 6 cut(s) 50, 604, 859, 906, 1029, 1313
TatI WGTACW 1 cut(s) 1054
TauI GCSGC 3 cut(s) 44, 209, 212
TfiI GAWTC 1 cut(s) 1214
TscAI CASTG 2 cut(s) 51, 199
TseFI GTSAC 3 cut(s) 125, 172, 1003
TseI GCWGC 3 cut(s) 553, 676, 715
Tsp45I GTSAC 3 cut(s) 125, 172, 1003
TspDTI ATGAA 4 cut(s) 71, 480, 1036, 1273
TspGWI ACGGA 1 cut(s) 533
TspRI CASTG 2 cut(s) 51, 199
VpaK11BI GGWCC 2 cut(s) 244, 866
XapI RAATTY 2 cut(s) 859, 1029
XceI RCATGY 1 cut(s) 1361
XcmI CCANNNNNNNNNTGG 1 cut(s) 254
XspI CTAG 4 cut(s) 9, 755, 987, 1017
ZrmI AGTACT 1 cut(s) 1056
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.